LOCUS       sequence-375959-        8315 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  acr-2(3.7k) fluorescent neural reporter driving nuclear mNeptune2.5 
            expression (refer to NeuroPAL paper for expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8315)
  AUTHORS   Yemini E, Lin A, Nejatbakhsh A, Varol E, Sun R, Mena GE, Samuel ADT,
            Paninski L, Venkatachalam V, Hobert O
  TITLE     NeuroPAL: A Multicolor Atlas for Whole-Brain Neuronal Identification
            in C. elegans.
  JOURNAL   Cell. 2021 Jan 7;184(1):272-288.e11. doi: 
            10.1016/j.cell.2020.12.012. Epub 2020 Dec 29.
  PUBMED    33378642
REFERENCE   2  (bases 1 to 8315)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.012"; journalName: "Cell"; date: "2021-01-7-
            7"; volume: "184"; issue: "1"; pages: "272-288.e11"
COMMENT     Sequence Label: pEY61
FEATURES             Location/Qualifiers
     source          1..8315
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6..22
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3990..4010
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4035..4055
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4077..4097
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(5906..5928)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6028..6047
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6125..6143)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6211..6315
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6316..6384,6385..7176)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6534..6553)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7347..7935
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7836..7855
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    8223..8244
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8259..8264,8265..8282,8283..8289)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8297..8313
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(8302..8315,1..9)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcctgcagt cccaaatcaa
      181 gccaagtaag tggtttctaa agccaattat tatttttttt gaagaataaa atttgaaaga
      241 aaaactatcg tccccccatt tctatactta ccaacacata taatactgta aaattccgtt
      301 aacaaactgt atttttattg tacttggagt gacatttttt aaaactttat tatattttcc
      361 gtttgattat aaacgtgaaa ataaatagag gttatcaaca aattatgaaa tcaagcaaat
      421 aaagttaaat tctcacaatt tcaaccactt ttgtcgattt tggtgtcgac attcttggaa
      481 tttcgcgttt tccattttcc gttgtttttt cattttataa aagtttcagc acctacattt
      541 ttcaatattt cttcaatgga gaataaatca gttgaatcag ttgaaaaaca aagtcagagc
      601 tgtggaagtc gaaatttaag agcttttttt ttaatttttt atagttgttg taaagcttat
      661 tagtataccg cacctactgt taatatgaac gatcaaatgt tactcttcct ttttcattaa
      721 aaaaaacttt cagtttgccc cgccggtgcg attccttata agatgccaca agttttgtta
      781 tgcgacccga caattgttaa tatctgtcca tacgactata aggtttggtt ttattcgcat
      841 tttattgttt tgtttttgga tcaactctat caaaaaaaca atattcgttc atgatactaa
      901 tgactaatga gttttcagtg cgttgaagca gcaaacggac aatatcttcc tgccgatggc
      961 cgatctcttt gctgcaagac caccactctg tactcgtttg cctcggtttt tggagaggct
     1021 aaaataagcc ctcggctagt gccaaatccc cctctttcag cgatcgaata tgtgcgtcgg
     1081 tattttttgt tcctcttatt ttttgtctta gacatcaacg ccgccgccgt aaaacagaag
     1141 acatgtttcc gtacttccag gtcactttga acgttcacac atccgccaaa gagcactcac
     1201 cggaaattag aactggagat cattttgtgc tgacaccata caaactctac gagccggcat
     1261 atttgaaaaa aatcaaactg ttcacagact ttggtcatgg ctcatttgtg catgttatac
     1321 tttttggtaa atctattgaa catgttcgga gtgttcatgc gagcgtaact aagttttgag
     1381 aaaaaaagaa tgagagaata actagggctt ttgtcttttt ttgtgaatat ctggttatgt
     1441 ttgaagatcc aatgtcaaca actgaaacaa tgcaacttta ttatgaccga ccaacatcca
     1501 atgggaaaac tatcaattta gatgagccaa ttccagacgg aggtttcaat aattaaaaaa
     1561 aaattaagaa taatcaaata acatgcgctt ttcaggattc atttctaaga aaatattcaa
     1621 tgcggctcca attacacaaa tagaaaaccc atcacgtccg ggtccaataa aagaatgtga
     1681 gaactttctt cttgaaaaat gaacatattg aattgcacca aacgcaaaca agaaactcta
     1741 aatttcagat cgaaaattgt ggattgtgct cattttcaag accacaagcc cgtttggtcg
     1801 agtttatatt ctttccacca aggatttgca cagcaagtat agatcagtca ccgatttttt
     1861 gaaatccgac acaggaaaca tgctaggaac tcctgtggct ggaacttatt tttatgtgag
     1921 ttttgttttg aaccctcaac tataaaattg tcggcccgtt ttgttttcag ctcaccaccg
     1981 actgattttc atttcaaaat taatgcagat ttctacgtgt tctttttcaa ataaatttat
     2041 atattttttg ttctctcaca aaataaataa tttcgagagt taagtgatca ttctcagaca
     2101 tattctataa ttgtccgtca ggaaacagat agaggaacct ttgataaata aattataaaa
     2161 ggccgacagg gaaatgcttc tccctcgact ctgttagaag cgaagaggca atttttaaaa
     2221 atttcccatt tttatgcgtt tttctgattt ccaaagtcac gcatcattta ccaaccgcaa
     2281 cgattcataa ggacgtaatt ttgattttct ggacgatttt tatatagttt tgatgtgaaa
     2341 ttcgctttat ttgtctgaaa gtggaataga tttagtgtgg atttaaccga aaaatgcaga
     2401 tcttttaaaa atttaaacca gtgttttgtt gccttatttg cgaaaaaaaa ttgtgatatt
     2461 tcgagtattg ctgcaatttg attagtaaaa tgttaggata taagtattta aaaagtataa
     2521 ttggttgaat tttctttttc atcaccgact tccaaaaata tagagatact gagttttcac
     2581 cattttttga atgtaaataa aaacattttg aacacttgtg ctgggattca atccaaatac
     2641 attcactgtc atttaatatt attatatatc aaaataaaac gcataacttt ttctgttttt
     2701 ttttaaattt tttcaaaatg taggtttggt aagttaaggt aaggttaggc aatgtaggta
     2761 aacacgagat gatactccac gttgaaaatt gttttcataa atacagttta gattgaactg
     2821 aaggatttta tcgtctaata gaatataatt ttttagttca agatttagga tttgttttca
     2881 ctttttttta gattcaagac atatatctcg caaaatgagt tttgtctttg agccatcttg
     2941 tcatcatagc acattgaatt ttgttgttcg caagacggaa tatatgttcc cagctgcaaa
     3001 ttttgtgtcg gatatttctt ctttagcgaa aggcgttaaa aaccaaaaat agtcgaagcg
     3061 tttgatatgt gtcccgttgg tttttcgtgt ccatctattc tgtttcgagt acacatgttg
     3121 cgacgcggca ctgatgaaaa agtgaaagag ctagtgtcac acacaaacac aaagtatgct
     3181 catgtgagtg tgtagatatg tgtagatgta tgtcctgaag gaataaaaag actttttgaa
     3241 gctacatttt taaacaaaaa aagacagaaa catgaagcac aactctctat tctgtcgaac
     3301 ggcgtgtgca actcacgcac acccaaaaat atgaaatata acattcgcac caacaaagcg
     3361 cgaatatcaa tgaatctcga gggaacgatg gctgcctgcc ggcgcgtgtt gctcttctgt
     3421 cattgggata actggcgggt ctgttgggtc cgtcctttag accatttgac tacactgccc
     3481 tgcatatgtg tgaaacgtcg tcgttgctgt tgggagctgt ctgtgtgtat gtgtgtgtgt
     3541 gtgagtatgg agcagtaaaa agctgccgat acgagaagcg ccgccccgga cggacggcga
     3601 tggcgcatcg ccaaatgtgt cattttagtt gtcttcacga accagagggc ccacgacgga
     3661 cgggcggcca cgcccacctc aaccagaata tccttccttc tcccacccac ccttgcggac
     3721 gcgcggatgc gctgaacaaa aagacgacga agaagaagaa gaagaagctg aacgaagaaa
     3781 aaagcatcaa aagaagtgaa gcggctctac caccccgacc accttccttc acaggaagga
     3841 cgccgttttc acccgggatt ggccaaagga cccaaaggta tgtttcgaat gatactaaca
     3901 taacatagaa cattttcagg aggacccttg gagggtaccg gttttcccag tcacgacgcg
     3961 tggtaccgag ctcagaaaaa atgaccgctc caaagaagaa acgcaaagta ccgagctcag
     4021 aaaaaatgac ggcacctaaa aagaaacgca aagtaccggt cgccgagaag acagccccaa
     4081 aaaagaagcg taaagttcca cgcgtcatgg gagtaagttt aaacagttcg gtactaacta
     4141 accatacata tttaaatttt cagcgcgctg tctccaaggg agaggagctc atcaaggaga
     4201 acatgcacac caagctctac atggagggaa ccgtcaacaa ccaccacttc aagtgcaccc
     4261 acgaagggga gggcaagccc tacgagggca cccagaccaa caggattaag gtggtggagg
     4321 gaggccccct gccgttcgca ttcgacatcc tggccacctg ctttatgtac gggagcaaga
     4381 ccttcatcaa ccacacccaa ggaatcccag acttcttcaa gcaatccttc gtaagtttaa
     4441 acatgatttt actaactaac taatctgatt taaattttca gcctgagggc ttcacatggg
     4501 agagagtcac cacatacgaa gacgggggcg tgcttaccgt tacccaggac accagcctcc
     4561 aggacggctg cttgatctac aacgtcaagc tcagaggggt gaacttccca tccaacggcc
     4621 ctgtgatgca gaagaaaaca ctcggctggg aggccagtac cgagaccctg taccccgctg
     4681 acggcggcct ggaaggcaga tgcgacatgg ccctgaagct cgtgggcggg ggccacctgc
     4741 actgcaacct gaagaccaca tacagatcca agaaacccgc taagaacctc aagatgccag
     4801 gagtaagttt aaacatatat atactaacta accctgatta tttaaatttt caggtctact
     4861 ttgtggaccg cagactggaa agaatcaagg aggccgacaa tgagacctac gtcgagcagc
     4921 acgaggtggc tgtggccaga tactgcgacc tccctagcaa actggggcac aaacttaatg
     4981 gcatggacga gctgtacaag taagcgccgg tcgctaccat taccaacttg tctggtgtca
     5041 aaaataatag gggccgctgt catcagagta agtttaaact gagttctact aactaacgag
     5101 taatatttaa attttcagca tctcgcgccc gtgcctctga cttctaagtc caattactct
     5161 tcaacatccc tacatgctct ttctccctgt gctcccaccc cctatttttg ttattatcaa
     5221 aaaacttctc ttaatttctt tgttttttag cttcttttaa gtcacctcta acaatgaaat
     5281 tgtgtagatt caaaaataga attaattcgt aataaaaagt cgaaaaaaat tgtgctccct
     5341 ccccccatta ataataattc tatcccaaaa tctacacaat gttctgtgta cacttcttat
     5401 gttttttact tctgataaat ttttttgaaa catcatagaa aaaaccgcac acaaaatacc
     5461 ttatcatatg ttacgtttca gtttatgacc gcaattttta tttcttcgca cgtctgggcc
     5521 tctcatgacg tcaaatcatg ctcatcgtga aaaagttttg gagtattttt ggaatttttc
     5581 aatcaagtga aagtttatga aattaatttt cctgcttttg ctttttgggg tttcccctat
     5641 tgtttgtcaa gatttcgagg acggcgtttt tcttgctaaa atcacaagta ttgatgagca
     5701 cgatgcaaga aagatcggaa gaaggtttgg gtttgaggct cagtggaagg tgagtagaag
     5761 ttgataattt gaaagtggag tagtgtctat ggggtttttg ccttaaatga cagaatacat
     5821 tcccaatata ccaaacataa ctgtttccta ctagtcggcc gtacgggccc tttcgtctcg
     5881 cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag
     5941 cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg
     6001 gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc
     6061 atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcggcctt
     6121 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
     6181 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     6241 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     6301 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     6361 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     6421 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     6481 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     6541 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     6601 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     6661 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     6721 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     6781 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     6841 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     6901 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     6961 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     7021 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     7081 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     7141 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     7201 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     7261 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     7321 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     7381 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     7441 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     7501 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     7561 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     7621 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     7681 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     7741 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     7801 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     7861 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     7921 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     7981 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     8041 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     8101 agcgatgaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     8161 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
     8221 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
     8281 cgtatgttgt gtggaattgt gagcggataa caatt
//
