LOCUS       sequence-375961-        7413 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  srh-79 fluorescent neural reporter driving nuclear CYOFP expression 
            (refer to NeuroPAL paper for expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7413)
  AUTHORS   Yemini E, Lin A, Nejatbakhsh A, Varol E, Sun R, Mena GE, Samuel ADT,
            Paninski L, Venkatachalam V, Hobert O
  TITLE     NeuroPAL: A Multicolor Atlas for Whole-Brain Neuronal Identification
            in C. elegans.
  JOURNAL   Cell. 2021 Jan 7;184(1):272-288.e11. doi: 
            10.1016/j.cell.2020.12.012. Epub 2020 Dec 29.
  PUBMED    33378642
REFERENCE   2  (bases 1 to 7413)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.012"; journalName: "Cell"; date: "2021-01-7-
            7"; volume: "184"; issue: "1"; pages: "272-288.e11"
COMMENT     Sequence Label: pEY35
FEATURES             Location/Qualifiers
     source          1..7413
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6..22
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2772..2792
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2814..2834
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(5004..5026)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5126..5145
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(5223..5241)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5309..5413
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5414..5482,5483..6274)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5632..5651)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6445..7033
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6934..6953
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7187..7204
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7321..7342
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7357..7362,7363..7380,7381..7387)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7395..7411
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(7400..7413,1..9)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcctgcaga ttcccagatt
      181 ggtttgaaga aacttgagta gacatttctt aaaaatgaag tttaaaaata gtaattataa
      241 cgatcaaaat atattttcgc aacatcagca gccaattgaa ataatgtttt aaacaatgta
      301 ttgtagctta tctctagtac aacttaacga cggattaatg gtaaaaaatt gttcaaaacc
      361 atgtttttaa tgctaaaatg atcaaatatc acataaaaac ttttaaaaat agattgaaaa
      421 tttattatct agagtcaaag tttttatcgc tcataagtat gtcgactaaa caataaaagt
      481 tccatcaaat taagttttca tttcgtaaca aacattattg gcgtttagcc ctgcgaagat
      541 gtgcttaatt tttcaaaatg gacaaatttc ccgaaaatga tcactgacta aactaaaaaa
      601 acagatatct aaaagtggct atttatgtta tgatatttgg taatttttga ttaatgtaag
      661 tgcatctaga tacattcccc acagacaccc atgattcatt aatacatgta atttgaaagc
      721 tttgtttaga tgaagtaatt atcaaatgat tagcgacaga agtcaacaag gaagtggtga
      781 tactgaaatc taacagtttt ttctgagctc gatgcccata aatttgcttt agaaatgatt
      841 gaagaaataa ataaagtttt ttttcatgtt tctgacgccg cacaaacagg taggttacct
      901 aattttttaa aacctaaaca tcccaagcga aaaactgttc ctaactaaaa agcatagagt
      961 atgatttcgt ttctagattt aaaggatata atggataaac gtttacagtc tgggaactgc
     1021 taaaggtcgt aaagaattta aagttttttt ccgtgtgaaa ataatttcaa aatgcgaggg
     1081 agtaaaaatt aaaaggggtc tgagaaaaat tttaagatcg tattttttgc ttttcaaagt
     1141 tgattaccgg cactaaagtt ttgtagtgga aaaatgtaat ggaaaatatg caataaaaaa
     1201 ccgtaataca aagttttttg ttctttttca tcaaatatta tcattaaaaa aactttgaaa
     1261 agcaaacttt aaagttacta acattatgag gaacctgtta gcgaaactat ttaaatttcg
     1321 aaacagttcc aaatattaca acttttgaag tttgaggtat attcgaaaga atcaggataa
     1381 aagaatgaaa aaacagaagc aaagagcttg ttgatgatta atatcacgag attgttaatt
     1441 ttgggtatta cctagtcatc ataaatatac gtgctttgtc tgataacatc gcacatcgag
     1501 tttgatacag actagataat tgactttttg attcgcctgg tgtatgcaga ttgatctttc
     1561 aaaatcatgt tcatccgagc aattgtgttg tgaaaaaaat tcgctgaaca ttttttgaaa
     1621 cgaatggggc aaaatcaatt gattctttta tgatcgatga taaatacatg ttttgacgcg
     1681 cacggaatcg gtacggtttc tgaaaaaaaa ttaactaggt gacaaatcga ttttctgatt
     1741 tagaaaaatt aatatagaat taggaaaatt ttttcatctg tttcgttctt atagaaattt
     1801 ggtattattt tcatttaaat tttcccagaa acgctcgccg aaaactttcg gtaaggaccg
     1861 gaaaacaatt tcatttgatt agaagaacgt ggagaaaact atacgttcaa gaaatttaga
     1921 aatagttcca ggaaacttta aattttaaag tttaaattgc ttaaaactta gttatttaat
     1981 atttatgttg ctcgaacact tccagaaaaa agggaaaaaa cgttctctta gagaagggtt
     2041 agaaaattta gaatttaatt ataattttga gtatgccagt cggagcacgc gcttcagcgc
     2101 gtgctccgac tggttcagat ataaaaaatt atatgtataa aattattaat tttaggtaaa
     2161 attttatgtg tcagaagtcg ggttatcaag ttttctttat aaaaatagat ttgaccttat
     2221 atttatttga gctggaattc aaatttttac cgtcattttt ttggcccatt ctctatcatt
     2281 cacaaaaaaa aaccccctag aactatgttt cacccttcac agaaagtcag agacacatta
     2341 ataaatcgaa tttctgattt attttatgta gtcaatttac attcgtttta tgtgtatagt
     2401 ttttatctag gtatatatca gagatctatt aatttactct tgtaatctca gaaatccacg
     2461 ttttcttcaa atttttgaaa catatcacca gttttgctaa acatttgatc gtcgctaatg
     2521 ttttcaaaat ttgcatctgt cgcgttaaac gttgatgtct ggcagccata aatcaagtta
     2581 cacgtgcaac agtaattagt tttcaaactt tttcatttga aacgaaataa aaaaacgttt
     2641 ttctcgttgt tcgagtgccc gggattggcc aaaggaccca aaggtatgtt tcgaatgata
     2701 ctaacataac atagaacatt ttcaggagga cccttgcttg gagggtaccg agctcagaaa
     2761 aaatgaccgc tccaaagaag aagcgcaagg ttccagtcga gaagatgact gccccaaaaa
     2821 agaaacgtaa ggtcccgcgg gtaagtttaa acagttcggt actaactaac catacatatt
     2881 taaattttca gcgcgctgtg tcaaaaggag aagagcttat taaggagaat atgcgttcca
     2941 agctctacct cgagggatcc gtcaacggac accaattcaa gtgcacccac gagggagagg
     3001 gaaagccata cgagggaaag caaaccaacc gtatcaaggt cgtcgaggga ggaccactcc
     3061 cattcgcctt cgacatcctc gccacccact tcatgtacgg atccaaggta agtttaaaca
     3121 tatatatact aactaaccct gattatttaa attttcaggt cttcatcaag tacccagccg
     3181 acctcccaga ctacttcaag caatccttcc cagagggatt cacctgggag cgtgtcatgg
     3241 tcttcgagga cggaggagtc ctcaccgcca cccaagacac ctccctccaa gacggagagc
     3301 tcatctacaa cgtcaaggtc cgtggagtca acttcccagc caacggacca gtcatgcaaa
     3361 agaagaccct cggatgggag ccatccaccg agaccatgta cccagccgac ggaggactcg
     3421 agggacgttg cgacaaggta agtttaaaca tgattttact aactaactaa tctgatttaa
     3481 attttcaggc cctcaagctc gtcggaggag gacacctcca cgtcaacttc aagaccacct
     3541 acaagtccaa gaagccagtc aagatgccag gagtccacta cgtcgaccgt cgtctcgagc
     3601 gtatcaagga ggccgacaac gagacctacg tcgagcaata cgagcacgcc gtcgcccgtt
     3661 actccaacct cggaggagga atggacgagc tctacaagcc taggataccg gtgggtgaag
     3721 acccaccaaa gccatctgcc aagggagcca agaaggccgc caagaccgtt acgaagccaa
     3781 aggacggaaa gaagagacgt catgcccgta aggaatcata ctccgtctac atctaccgtg
     3841 tcctcaagca agttcatcca gacactggag tttcctccaa agccatgtct atcatgaact
     3901 cttttgtcaa cgatgtcttc gagcgtattg ctgctgaagc atcccgtctt gctcactaca
     3961 acaagcgttc cacaatctca tcccgcgaaa ttcagaccgc tgtccgtctg atccttccag
     4021 gagagcttgc caagcacgcc gtgtctgagg gaaccaaggc cgttaccaag tacacttcca
     4081 gcaagtagga attccaactg agcgccggtc gctaccatta ccaacttgtc tggtgtcaaa
     4141 aataataggg gccgctgtca tcagagtaag tttaaactga gttctactaa ctaacgagta
     4201 atatttaaat tttcagcatc tcgcgcccgt gcctctgact tctaagtcca attactcttc
     4261 aacatcccta catgctcttt ctccctgtgc tcccaccccc tatttttgtt attatcaaaa
     4321 aacttctctt aatttctttg ttttttagct tcttttaagt cacctctaac aatgaaattg
     4381 tgtagattca aaaatagaat taattcgtaa taaaaagtcg aaaaaaattg tgctccctcc
     4441 ccccattaat aataattcta tcccaaaatc tacacaatgt tctgtgtaca cttcttatgt
     4501 tttttacttc tgataaattt ttttgaaaca tcatagaaaa aaccgcacac aaaatacctt
     4561 atcatatgtt acgtttcagt ttatgaccgc aatttttatt tcttcgcacg tctgggcctc
     4621 tcatgacgtc aaatcatgct catcgtgaaa aagttttgga gtatttttgg aatttttcaa
     4681 tcaagtgaaa gtttatgaaa ttaattttcc tgcttttgct ttttggggtt tcccctattg
     4741 tttgtcaaga tttcgaggac ggcgtttttc ttgctaaaat cacaagtatt gatgagcacg
     4801 atgcaagaaa gatcggaaga aggtttgggt ttgaggctca gtggaaggtg agtagaagtt
     4861 gataatttga aagtggagta gtgtctatgg ggtttttgcc ttaaatgaca gaatacattc
     4921 ccaatatacc aaacataact gtttcctact agtcggccgt acgggccctt tcgtctcgcg
     4981 cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct
     5041 tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc
     5101 gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat
     5161 atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gcggccttaa
     5221 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
     5281 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     5341 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     5401 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     5461 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     5521 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     5581 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     5641 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     5701 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     5761 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     5821 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     5881 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     5941 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     6001 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     6061 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     6121 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     6181 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     6241 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     6301 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     6361 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     6421 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     6481 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     6541 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     6601 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     6661 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     6721 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     6781 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     6841 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     6901 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     6961 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     7021 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     7081 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     7141 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     7201 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
     7261 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
     7321 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
     7381 tatgttgtgt ggaattgtga gcggataaca att
//
