LOCUS       sequence-375965-        7679 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ceh-1 fluorescent neural reporter driving nuclear CYOFP expression 
            (refer to NeuroPAL paper for expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7679)
  AUTHORS   Yemini E, Lin A, Nejatbakhsh A, Varol E, Sun R, Mena GE, Samuel ADT,
            Paninski L, Venkatachalam V, Hobert O
  TITLE     NeuroPAL: A Multicolor Atlas for Whole-Brain Neuronal Identification
            in C. elegans.
  JOURNAL   Cell. 2021 Jan 7;184(1):272-288.e11. doi: 
            10.1016/j.cell.2020.12.012. Epub 2020 Dec 29.
  PUBMED    33378642
REFERENCE   2  (bases 1 to 7679)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.012"; journalName: "Cell"; date: "2021-01-7-
            7"; volume: "184"; issue: "1"; pages: "272-288.e11"
COMMENT     Sequence Label: pEY67
FEATURES             Location/Qualifiers
     source          1..7679
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6..22
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     RBS             528..536
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             3038..3058
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3080..3100
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(5270..5292)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5392..5411
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(5489..5507)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5575..5679
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5680..5748,5749..6540)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5898..5917)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6711..7299
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7200..7219
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7453..7470
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7587..7608
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7623..7628,7629..7646,7647..7653)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7661..7677
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(7666..7679,1..9)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcctgcagt cttcagcgat
      181 ctcaccgatt tcccagtctg atctttttct tgttgtccat agttttattc actgactttc
      241 agtgtctcaa ttaggttgac aagtgctctc gtaactttaa atctccgatg attttgaaat
      301 atctaaaatc taaatccagt tatcaccagt cattcaattt atgtgtatta taaagtgcct
      361 aaaaatgtga atcttcattt ttcggcacgt cgtcacctct gacaaaactc atctttgtag
      421 tataggaaac aaaaatgtac ttgatttcat ttaaaaatcc caaggtcttt atctacagcc
      481 cctccaaaat tttcaatttt tttcactatc tgtttttccc ttgctccacc tccttaaacg
      541 tatcccgtaa tcaaaatcta taatctatca tattgtattc tccagcaaca aatgcaacca
      601 aatgtattaa aaatgtttaa aaatttataa agattattgt cacacttttc acatccaatt
      661 accagtctga ctctcacgtt tccagtgaat aacagcgtgt ttcaaaatgt catgtgctaa
      721 taatattgaa catattagca aaaacctcat tgaaattcta caaaactgca tcgagtacgt
      781 tttaatgaat acaggtaaaa tttttgaaaa ttcccaaaaa atgttttcat tattaatcat
      841 tttttgattg aattttaaat gttttcccag aatatgaaca acgaatatat atatataaag
      901 cagtcaattt tttaatgatt tttaatgact gcacagttac aatattaaaa tcattaaaaa
      961 ttgtataagt caatacaaat tgttaaacat attttctcta attttatctt ttttattgtc
     1021 aaatttttaa tagcactaca ttttactgtt gtatttttga ctacggttta aaatgagtcg
     1081 aaaaatgagc attctcatga aaataattta tataagcttt tctttgccgg ttgccaaaaa
     1141 ttttaaagtt atgttgttcg atttgcttat tttcgaagaa ttgaggtgtt ttaaattgct
     1201 ttatcaaaat ccgacccgca agttgttagc tcctccaatt gtgaaaatta aaaaacttta
     1261 ccagaaactg cgtgtctttg atttatgctt ataggattaa atgtaagagt tatacacaat
     1321 taaaattctt ttgaccgaat gaattttata caggtttaat gaattttcat atcactttta
     1381 aacgtatgtc caaaaaacta acatctaggt gttctaaaag tctaattatc tctacgtcca
     1441 aaattattgt ttgaatacta tccataaacc tgtcatattt taagaaattt acgatttctt
     1501 ggcaaataat ttgatttgct ttttttttta cttcagctca catgtaactc tataaagcga
     1561 tatataaata aaactccata ctcagacgca gcctaaattg ttctcttcaa aagcatacac
     1621 tgtacttttt catttcgttt cctcccgtta acaatataaa aagtgctcca tcattcctca
     1681 actattaaaa attgtctcct cttgtcaata gttcttgttc ttccgtcttc aactcctccg
     1741 caactttatt tattcccatt ttctcttttt tagttctaaa aaatttatgt ttctgtcttt
     1801 atttctcatc atgtcacaat caaatagaag aagctccggt aggcggtaaa cttctgtgtg
     1861 cttggagtac aatagaagcc gaaaccaata caataaaatg acacagaaga gacaaaaaga
     1921 atagttcatc tcattagctc agttgctctg atttgctatt atgaataatc caaagggtct
     1981 gaagaacccg atagcttttg tgtttgtagt tgttagcgaa gagaaagagg cgtaacatat
     2041 tttcatatag taagcaatta ttacgcaagt gttgtttgga agattgtaaa tgagatgagc
     2101 aacgaggcga agaatggggt ctccccggca gtcgccgcag atttcgttgc ccaatcaaaa
     2161 ctaccccacc gactccaccc catctgctgt cagtttgatt ttgagccccg atccaatagg
     2221 ttctcgtgag agttttttgt cgcgtttcat ttcttgatga tttttcttga ttgaaaaaac
     2281 tattgtggca gtggttttaa ctatgcccat aactgttgga atttcatcag gtatgaaaat
     2341 tccatcaagt tttatcatat gttactgttc ccaggatcgt tttttgagat ttcaaaacag
     2401 caaagtgatt taaattttac atttttagag ttaggagtaa tcatatctat gccccatttt
     2461 ttaaaattca acagatcata tatcagttct taatttacat atccacaaat cttcaattga
     2521 caaagtacat atttgtattt tcttcatata ttatttattt tatgtttagt agagattatc
     2581 aattgacatc ttcaaaatga accgagatat aacctcacaa attgaagtca cctattacgg
     2641 gtcccgcaag tatcattcct gttaacaaac aaaaccagct gttgtaaacg ccagcgtgct
     2701 actttttaat aaaaccaaac acactcacat tttcaatttt tactgcctta accaaaatgt
     2761 cccagaaaaa ccactaattt ctattactga gacattttgt aaaatatcgt gaacattctc
     2821 tctttctcac tctcgttcgt tttttttgtt gaattccaat tttggagttc aaggccaaat
     2881 tgcacttttt tgtttgtaat acaagctttt catcttgaca gctcccggga ttggccaaag
     2941 gacccaaagg tatgtttcga atgatactaa cataacatag aacattttca ggaggaccct
     3001 tgcttggagg gtaccgagct cagaaaaaat gaccgctcca aagaagaagc gcaaggttcc
     3061 agtcgagaag atgactgccc caaaaaagaa acgtaaggtc ccgcgggtaa gtttaaacag
     3121 ttcggtacta actaaccata catatttaaa ttttcagcgc gctgtgtcaa aaggagaaga
     3181 gcttattaag gagaatatgc gttccaagct ctacctcgag ggatccgtca acggacacca
     3241 attcaagtgc acccacgagg gagagggaaa gccatacgag ggaaagcaaa ccaaccgtat
     3301 caaggtcgtc gagggaggac cactcccatt cgccttcgac atcctcgcca cccacttcat
     3361 gtacggatcc aaggtaagtt taaacatata tatactaact aaccctgatt atttaaattt
     3421 tcaggtcttc atcaagtacc cagccgacct cccagactac ttcaagcaat ccttcccaga
     3481 gggattcacc tgggagcgtg tcatggtctt cgaggacgga ggagtcctca ccgccaccca
     3541 agacacctcc ctccaagacg gagagctcat ctacaacgtc aaggtccgtg gagtcaactt
     3601 cccagccaac ggaccagtca tgcaaaagaa gaccctcgga tgggagccat ccaccgagac
     3661 catgtaccca gccgacggag gactcgaggg acgttgcgac aaggtaagtt taaacatgat
     3721 tttactaact aactaatctg atttaaattt tcaggccctc aagctcgtcg gaggaggaca
     3781 cctccacgtc aacttcaaga ccacctacaa gtccaagaag ccagtcaaga tgccaggagt
     3841 ccactacgtc gaccgtcgtc tcgagcgtat caaggaggcc gacaacgaga cctacgtcga
     3901 gcaatacgag cacgccgtcg cccgttactc caacctcgga ggaggaatgg acgagctcta
     3961 caagcctagg ataccggtgg gtgaagaccc accaaagcca tctgccaagg gagccaagaa
     4021 ggccgccaag accgttacga agccaaagga cggaaagaag agacgtcatg cccgtaagga
     4081 atcatactcc gtctacatct accgtgtcct caagcaagtt catccagaca ctggagtttc
     4141 ctccaaagcc atgtctatca tgaactcttt tgtcaacgat gtcttcgagc gtattgctgc
     4201 tgaagcatcc cgtcttgctc actacaacaa gcgttccaca atctcatccc gcgaaattca
     4261 gaccgctgtc cgtctgatcc ttccaggaga gcttgccaag cacgccgtgt ctgagggaac
     4321 caaggccgtt accaagtaca cttccagcaa gtaggaattc caactgagcg ccggtcgcta
     4381 ccattaccaa cttgtctggt gtcaaaaata ataggggccg ctgtcatcag agtaagttta
     4441 aactgagttc tactaactaa cgagtaatat ttaaattttc agcatctcgc gcccgtgcct
     4501 ctgacttcta agtccaatta ctcttcaaca tccctacatg ctctttctcc ctgtgctccc
     4561 accccctatt tttgttatta tcaaaaaact tctcttaatt tctttgtttt ttagcttctt
     4621 ttaagtcacc tctaacaatg aaattgtgta gattcaaaaa tagaattaat tcgtaataaa
     4681 aagtcgaaaa aaattgtgct ccctcccccc attaataata attctatccc aaaatctaca
     4741 caatgttctg tgtacacttc ttatgttttt tacttctgat aaattttttt gaaacatcat
     4801 agaaaaaacc gcacacaaaa taccttatca tatgttacgt ttcagtttat gaccgcaatt
     4861 tttatttctt cgcacgtctg ggcctctcat gacgtcaaat catgctcatc gtgaaaaagt
     4921 tttggagtat ttttggaatt tttcaatcaa gtgaaagttt atgaaattaa ttttcctgct
     4981 tttgcttttt ggggtttccc ctattgtttg tcaagatttc gaggacggcg tttttcttgc
     5041 taaaatcaca agtattgatg agcacgatgc aagaaagatc ggaagaaggt ttgggtttga
     5101 ggctcagtgg aaggtgagta gaagttgata atttgaaagt ggagtagtgt ctatggggtt
     5161 tttgccttaa atgacagaat acattcccaa tataccaaac ataactgttt cctactagtc
     5221 ggccgtacgg gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca
     5281 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc
     5341 gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag
     5401 agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga
     5461 gaaaataccg catcaggcgg ccttaagggc ctcgtgatac gcctattttt ataggttaat
     5521 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
     5581 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     5641 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     5701 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     5761 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     5821 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     5881 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     5941 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     6001 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     6061 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     6121 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     6181 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     6241 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     6301 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     6361 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     6421 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     6481 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     6541 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     6601 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     6661 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     6721 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     6781 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     6841 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     6901 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     6961 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     7021 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     7081 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     7141 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     7201 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     7261 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     7321 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     7381 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     7441 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
     7501 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     7561 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
     7621 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaatt
//
