LOCUS       sequence-375966-       13257 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  E. coli - Eggerthella lenta shuttle plasmid (KanR), native 
            promoter-dopamine dehydroxylase from dopamine-metabolizing E. lenta 
            A2 strain (R506S mutation) with transcriptional regulators 
            DadR/DadS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13257)
  AUTHORS   Dong X, Guthrie BGH, Alexander M, Noecker C, Ramirez L, Glasser NR, 
            Turnbaugh PJ, Balskus EP
  TITLE     Genetic manipulation of the human gut bacterium Eggerthella lenta 
            reveals a widespread family of transcriptional regulators.
  JOURNAL   Nat Commun. 2022 Dec 9;13(1):7624. doi: 10.1038/s41467-022-33576-3.
  PUBMED    36494336
REFERENCE   2  (bases 1 to 13257)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-12-9"; volume: "13(1):7624. doi"; pages: " 
            10.1038/s41467-022-33576-3"
COMMENT     Sequence Label: pXD70DAmt1-DadR/DadS-Pdadh-DadhABC(R506S, A2)
FEATURES             Location/Qualifiers
     source          1..13257
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    264..283
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     primer_bind     complement(440..458)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        526..630
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(631..699,700..1491)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(849..868)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1662..2250
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2151..2170
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2431..2502)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             5555..6349
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     terminator      complement(6472..6499)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
ORIGIN
        1 gccgtcgacg gctcgcctgc ggccgcgtgc gacacggcgc ttcgctgcaa gtgccggctc
       61 gcggccgagc gctgcgggct cgaccggcgc gagctgggcg cgctcatcct gtacgtggag
      121 gagcgcggct tcgaggcgat ccagggcgag ctggcgctga cgcgcgacga ggtggccgac
      181 gtgctgggca gcgtgtaccg caagctgggc gtgggcgaca agcaggaggc gctcgatttc
      241 atccattccg tttcggaatg aatctagaag cggccgcgaa ttcagaatag taatacagga
      301 tccgaatcgt ttcagttgcc gccggattaa taatcaaata accgttccta aaaaatgccc
      361 tcttgggtta tcaagagggt cattatattt cgcaaaaaac cccgcttcgg cggggttttt
      421 tcgccttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
      481 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
      541 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
      601 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
      661 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
      721 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
      781 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
      841 aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt
      901 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
      961 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     1021 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     1081 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     1141 ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa
     1201 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     1261 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     1321 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     1381 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     1441 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     1501 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     1561 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     1621 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     1681 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     1741 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     1801 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     1861 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     1921 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     1981 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     2041 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     2101 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     2161 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     2221 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcccg agatgcgccg
     2281 cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc
     2341 attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc
     2401 gaggtgccgc cggcatgagc tcctctagta gagagcgttc accgacaaac aacagataaa
     2461 acgaaaggcc cagtctttcg actgagcctt tcgttttatt tgaaaaagaa aagccccagc
     2521 aagcttggcg gcagcagggg cattcgagac aaggagatta tagcatggcg gcaaacgctg
     2581 gcgagacgaa ggtgaagctc tgggagggca tctgctaccc agagaacatg cgcgatgact
     2641 ggcaggacga gatagacgac attcttcaag tgcctttcgc ttatgcggtt cacgacatag
     2701 accatgacca gaagagcaag cagcgcaaga cgcatgcgca cgtcattgtt gtctggggcg
     2761 gcaacacgac acgcaaggca atcatcaacg tcttgaaccg attgagcgcg gacggcaaga
     2821 agtgctgttc gtcagccgag ccaatcaaca acatcaggca tgcgtatgac tatctgattc
     2881 acgacacggc ttcctgtcgc aagaagggca aggagctgta tccggtcgaa gcacgcatcg
     2941 agggaaacaa cttcgacatt ggtcagctcg aacagctctc gactaaggac aagcaggaca
     3001 tgctcttcga gttggtcggc ttcatcatgt gcgagaggtt cgagaccatc aacgatttca
     3061 cggctgcggc gctgagggag tttcccgagc agtaccgcga aatcatcgtg gggtacaact
     3121 cgattctcga gcgctattgt cgcgggaact atctgaacgc cgaacgtaag cgcaagtgcg
     3181 tggaacagtc aggtgccaaa gagtagtcgg gtcaatgcag caatgcgtga ctacggggcg
     3241 gcgtggtctg cgctcgcgca gatctaagcc gactcgttca cggattgcgg aattgacgcg
     3301 acggccaacc gaatagacca gacttgatta agggtcgatg gacgctctgc tccatcgacc
     3361 ctttccttgc ccgtgtctcg actggatgag acctgcagaa tcgggcggct cattattttt
     3421 cgctaactac gtccgaaaaa taatgtgtcc gaaaaataat gtgacaaatg acaaatgaca
     3481 aatgaccaca ggggggccac tacgacggcc cccctaatag tgccgtgtgc cgtgtgccga
     3541 accgcaggta gattgagggg tggttcggag gggcgaagtc cctccgttcg gcgctcgcgc
     3601 cgatggcccg agggccgtga gcgaagccgc gaacgggcaa gggctggggg gtctcggggg
     3661 gaacgcccga gcgggtgcag ggctggccct gccacgtcct tagcgtagct aaggtctaac
     3721 gtgctagact ttagtcgatt aagtctgtac ggttcggagg tttccatggc tcacatcgcg
     3781 aaatacaagg ctccatcggt cgggcacatg cttgcccact accgccgcga ccgcagcagc
     3841 ctggagcgcg acaacatcga ccccgagcgc atcaagaacg acttggtggt cggccactac
     3901 acgaacaagg acggcaagct cgttgtgggg cgcgtggagc cgcgcgatgg cgagccgaac
     3961 tggggcacgg tggagagccg catcgagcgc gtgaacgagg cccagaaggc cgcagggaag
     4021 cgtgccacgc gcaaggatgc ggtcgttatg gctgacgttg ttgtgacgct tcccgacaat
     4081 gtgcgcaagg gcgatgagga caggttcttc cgtctcacgt actggtacct gtcgaagaag
     4141 ttcggcatcg agaacatgat gggcgggtac gtccacaagg acgaggtgtt gaaggatggc
     4201 acgcccgccc gcgaccacat gcacgtgccg ttcacgccca tcctagatgg acggttcaac
     4261 tacaagcaga tgtgcccccg cagcttctac cggagcatgc acaaggagct tggggattac
     4321 ctagaggggc gcatgggcta ccgccctgca atcgagcttg acgaggagac gcgggcgcag
     4381 cgtgtataca cggacaagtc cgtggatatc gacaaggtac gctgtgcggt cgataaggcc
     4441 gtggttgaac ctgcgaagga cgaagcggcg cgaatcgtcg cggcggctag ggaagaagcc
     4501 gccgctttac tgaatgatgc cgaaacgcgt aaggcagagc ttgttaccga gattgccgac
     4561 aaggagggtg accttgccga gcttgacagc cagctcgagg acgtgaagct cgatatcgaa
     4621 gatgagcaag accgattgga gtgtcttcgg caacgagcgg acggagttgc gcgagacatt
     4681 ggagagcttc aacccatcgc taccgaggtt cggggctggg aagctgcggg aaaagctgag
     4741 cgcggcgcaa tcctcgataa catcgttgtt aagtgcgatg gactcgcgag ccgcatcaga
     4801 gcaggagttg cgggaatgcg aatcaaagtg gaagagctta gaagtcgaat atcgcgcccg
     4861 ctcgaatatc tgatgcgcag ggaacagcca aggcaacaga gccttaatga cgtgctgcgc
     4921 gatgcgcagc gggcggcgga tgcgtggaac cgcgaccacg ctgcaccgca gaggacgtat
     4981 cgcgggcggg ctagatgatg tgttatagta gtacctgtaa tacacgacaa ggaggtgcga
     5041 cgcgaaaaaa ccccgctgaa gcggggtttt ttgcggagct tcgcttccac gatgcgcatg
     5101 attcggaggc cccggcgatc gccggggcct ccgtgcgttg tgtgtcgatt tgggatgcag
     5161 ccagatgggc ttcctcggcg cgaaccggcc gagatttccg aatgcagacg gtctcgcttg
     5221 gacgatgcgc tttcgaataa tcccggcgga gcgggcattc cagtcttctt atcaatatat
     5281 ctatagaatg ggcaaagcat aaaaacttgc atggactaat gcttgaaacc caggacaata
     5341 accttatagc ttgtaaattc tatcataatt gtggtttcaa aatcggctcc gtcgatacta
     5401 tgttatacgc caactttgaa aacaactttg aaaaagctgt tttctggtat ttaaggtttt
     5461 agaatgcaag gaacagtgaa ttggagttcg tcttgttata attagcttct tggggtatct
     5521 ttaaatactg tagaaaagag gaaggaaata ataaatggct aaaatgagaa tatcaccgga
     5581 attgaaaaaa ctgatcgaaa aataccgctg cgtaaaagat acggaaggaa tgtctcctgc
     5641 taaggtatat aagctggtgg gagaaaatga aaacctatat ttaaaaatga cggacagccg
     5701 gtataaaggg accacctatg atgtggaacg ggaaaaggac atgatgctat ggctggaagg
     5761 aaagctgcct gttccaaagg tcctgcactt tgaacggcat gatggctgga gcaatctgct
     5821 catgagtgag gccgatggcg tcctttgctc ggaagagtat gaagatgaac aaagccctga
     5881 aaagattatc gagctgtatg cggagtgcat caggctcttt cactccatcg acatatcgga
     5941 ttgtccctat acgaatagct tagacagccg cttagccgaa ttggattact tactgaataa
     6001 cgatctggcc gatgtggatt gcgaaaactg ggaagaagac actccattta aagatccgcg
     6061 cgagctgtat gattttttaa agacggaaaa gcccgaagag gaacttgtct tttcccacgg
     6121 cgacctggga gacagcaaca tctttgtgaa agatggcaaa gtaagtggct ttattgatct
     6181 tgggagaagc ggcagggcgg acaagtggta tgacattgcc ttctgcgtcc ggtcgatcag
     6241 ggaggatatc ggggaagaac agtatgtcga gctatttttt gacttactgg ggatcaagcc
     6301 tgattgggag aaaataaaat attatatttt actggatgaa ttgttttaga tcgttaaggg
     6361 atcaactttg ggagagagtt caaaattgat ccttttttta taacaggaat taaataataa
     6421 aaaagccgga ttaataatct ggctttttat attctctctc tagtatataa acgcagaaag
     6481 gcccacccga aggtgagcca gtgtgagtcg actagtttag tacatcagga ccactgggta
     6541 gcccagctgg tagatcagca cgcgaaacat cacggaggtg gccagcgcgc cgatggcggc
     6601 gacgaccatg agcgtcttgg aggcgcccac cttcttcttc gccacgaagg cgcaggccgc
     6661 agccacgatc acggagacga tggagcccca gaacatgggc gctcccgcgc cggacaccat
     6721 gacggtcatc aggctgtcga cgtgcgtggg ggccgtggtc atcgatgtcg ggtcggcgta
     6781 gtagccgaat tgcgcgatct tcgccatggc gcaggcgatg acgaacacca cgtcggccac
     6841 gatctgcacg agggccgcga tgaacgtcag ctggatgccc gtcgcctcga cggcctcgtc
     6901 cttcttcagg atggagatca gccagacggc cgcggcgccc aggaggcacg cgttgcccag
     6961 atagaacgcc acgagcgcca ggccccacgc gggacgggcc ggcatgaggt aggagtgcgc
     7021 ggtggcgacc atcatgagca cggcaaccgc gagggtcgcc caggccagcg ccttgggaac
     7081 gggcttgccg cctcgaagga ccacgaacca tgcgacgatg acgatggcca gcgccacgca
     7141 tccgatgagc tcctgggtga tgcccgacgt gatgtgcccg aagccgttga agatgcgctc
     7201 ccagtgctcg aggtgcagga aggctccgat gccgcctacc accagcgaga cggcggacgt
     7261 gatgagcgcg gtcatctgca ggttcctgcc ctggcccttg agcgccagga tggacatgcc
     7321 gcccagcgtg ccgcaggtca agcatacgaa cagcgtgaag atgacgagag gccactgcgt
     7381 ttccattact catcacctcc ttcccagtga tggtcgcgta ggatgtagga gtacgacggc
     7441 ttgttgccca cgtcgggcag cttgtgcacg tcggcgtcgg tgaacggctc caggaactcc
     7501 accatcttgc ggcgcttgcc cagcgggtcc tcggagccct cgaacgactc gtagccggcc
     7561 tcggtgtcgc cgatccagcg ggcgttgccg ccgcactggc tgacgcactg gggcagctcg
     7621 ccctgcgaca gcttctgctc gcacagcgtg cacttctcga cgaccttctc ctcctcgttg
     7681 aggtagcgga cgccgtaggg gcaggccatg acgcagaact ggcagccgat gcacttctcc
     7741 ttgtcgatct ggatggagcc gtcctcggcc acgtgcgacg cctcggtggg gcacaccttc
     7801 acgcactcgg ggttctcgca gtgctggcag ctgatgggca ggaagtacat gtacacgtcg
     7861 gggtactgcc ctgatccgcc cgggatgggg ttcggcccca cgcgcaccac tttgttccag
     7921 tagctgccga tgggcacgtt gttgaccgcc ttgcaggcga ccgagcacgc caggcagccc
     7981 acgcaccggt tgaggtcggt gatgatagcc ttcgtcatgg tttactccct cccttcgtag
     8041 gtaggcagcc attccttcag acgggggtcg tcggaggtat ggatgatggg cgtgccggtg
     8101 gagtcgcacg gcacggggtt gttgaacggc gagttctcgg gcgtggcctt gtagatcttc
     8161 acctggtagg cgcgcaggtt ggacgtgccg gagtgcgggt cctgggcgta gtggtcgatc
     8221 agctggttca cctgggagaa ctgccagccg tggccggcgt ccttcacctc ggggtaccac
     8281 cacgtgtgct cgaggttgat gacgtcttcc ttcacgccgt agtacaggtc agccacttcg
     8341 cggatcttgc cccactcggt ttcgatccac acccagtcgc cctgctcgat gccgtactcg
     8401 gcggccgtct tcgggttgat ctccacgcgc ggcacgggcc acagctcgcg gcaccaggga
     8461 agctggcgat gctcggagtg gaagtacacc gggatgcgac ggccggtggt ggcggtcagc
     8521 gggtactcct tgatgcggtc gccgtccttc gggccgtgag gcggctcggt gtaggtggga
     8581 agcctccact cgggatggtc gggatggtgc gattccatga cggtggacca gatctcctgc
     8641 ttcatcgtcg gggtgaacca gcccggcttc cagtcgccga tgcccttgcc ggccgtgtag
     8701 tcgaggcggc cccacacgcg gtcgcgtgcg cgcatcgcgc cggtctggta gcggcggtac
     8761 gtgccccagt tgcgcggctc gatgtccttc gcctgccacc agccgtgctc ctggaaggcc
     8821 ttgacgtact cgtcccacgt cttgtacttg ggcgtgacct cggccatcgg aacgccgtgg
     8881 cgctcgaacg tgggcttgcc gttctcgacg tgccacgtgg tgtaggtcag ctcgtcgtcg
     8941 gtgagcagct tgatggagtc gtccagctgc cagttgatgt cgggccactc gttgcccggc
     9001 tcgtcggtcc acttccagtt catgcggcgg gcgaggtcca tgacgatctc gggatcgtac
     9061 ttggcttccg cgggcggctg cacgcacttg accgtggcgc ccatggcgcc ggcggaacct
     9121 tggctggcgc gcggcgagtt gagctcgatc caatgggcca ccggcaggat gatgtcggcg
     9181 gtgtcgttct gcggcgtgtg ccacaggttg aggtccacgt agaagtcaag cgagcacagg
     9241 gcctcccagg cgcgcgtggt gttcgtctgg ctcatgaagt tgccggactc gttccagagg
     9301 gcgcgcaccg ggtaggggtc gcccgtgatg acggcgtccc acagcgaatg gctgtcgcac
     9361 cagtactgcc accagcccag aagcgggaac ttgtcgatgc cgatctgctt gcggttgact
     9421 tcctccggcg gggtggtggc gccgggggcc caggcgctga agccctggag gtcgccgtcg
     9481 atggggacga tggtcgggcc gcggttgccg cccggggtgt ccatgttgcc ggtgatgccc
     9541 acgaggttgt cgaaggcgct gcagttctgg atggcgctgc agaagtgctc gaccgccagc
     9601 atgtactgga tgccgccgtt gccgtagccg gtggacgggt cgatgcgcgt gccgtaggcc
     9661 gtggccgccg cctcgatctc ggaggcgggg atgccggtga tctcggccgc cacctcgggc
     9721 ttgtactcgg cggcgcgggc cttgtagtgc tcccacaccg gcttgacctt cacggtcttg
     9781 ccgtccttca gcgtgatctc gaactcgcct tccagcgcgg ggtcgatggc ggggtcgaac
     9841 ggcatgggat cgggcagcca gccctgcgtc tggcccggca gcaggttgtc ctgcgcggcc
     9901 tcgcggcctt ggcgcgcctt ctcggagtcg tagttctcgc cctcccagaa gccgccggtc
     9961 tcgtcgatga cgccttcctg gtcggcgttg aaccaggtga acgcgccgta ctcgtgctcg
    10021 acgccctcgg ccttcagctt ctcccagttg ttgtcgtaca cgaggaactt ataggggctg
    10081 ccgccctcct tgatgtcgct ttccttgagc agcgcggttt tcacgtccca gtaggaaccg
    10141 tcggtgcgca cggtggggaa gccggagggc tccatgtcct cgcacacgag gaacggggcg
    10201 ttcgtccact tcttcacgta cagctcgtcg tagagcttct tctcgatgat gacgttcgtc
    10261 caggccaggg ccagggcgcc gtcggtgccg gggcgcaggt gctgccagta gtcggcttcc
    10321 ttgcccatgt tggccatgcg ggggtcgacg ctgatgtgca cgtcggcgcg cgatgccacg
    10381 tccaccgtgg tgcggcagga gtcgtcgtag ttggacagct cggacgcgcc gccccactgc
    10441 acgtacactt tcgggcgcgt gatggtttcc atccacgaca tggcgaactg cgacaccatg
    10501 gtggtggcga agtggcgcgg gcccttgcag atctgccagg cctcaacgtt gttcggcgtt
    10561 tccagcatgt tcttcaggat ggactccgag tgcatgcacc agatgcgcga cgtgcccacc
    10621 tggcaggcga tggcctcgcc gccgtgcttc gccttgatgt ccatgaagtt gtccacgatg
    10681 gactgcatgg cctcgtccca cgagatgcgc tgccagccgg gctcctcgcc cttcgggttc
    10741 gtgcgcttca tgggatagtg cagacggtcg gggtggtacg ccgcctggat ggacgactgc
    10801 gacttcgtgc agcagttgcc catggactgg aacgctccct cgtcgccctc gacgcgtatg
    10861 gcccgcccgt tctggacgat gaccttgacg ccgcactcca tcttgccgca gccgcggcag
    10921 cacgttttca cggccacggt gtcgttgccg gacacgctgc tgtacgtctc ttcggccagc
    10981 gcggtgtgcg tcgaagcgcc gaacgccgcg gctgccgcac cggtgatggc ggcggtcttg
    11041 acgaacgtgc gtcgtgacat ggtcaggtta cccatactcc tcctccttct ttctcctcgt
    11101 tctcgtcttg ctcgtcttcg ttcgtgcgat cggggagggg ggctgccggg tgaagcctcg
    11161 ggctccgaac cggtgcaaag tccccgtgtc gcttgggccg accatacgga tgcgaggggg
    11221 tgcagagcat cgtccaaagg ttggaaagca ggttcgaccg catcataaat tcttatgatc
    11281 ttgtgatcat gaaaaattcg atagtgcttt gaactgggga aactttaacc gtaggtcata
    11341 aattattctg aaactagaat agtttcctca aagagggccg ctcatctttt ccgtataatc
    11401 gaacccgtcg acagaggggt cggcgcgagc gacgttatgt acgggctcac agggggtggg
    11461 gcatggaaaa gcttcggagt gcatgggata ccgcgttggg gacgagcctt tccctcaagc
    11521 gcatcgcggg cgcggccttg caatggcttt ggatgctcat gatgttctac agcatcgtgc
    11581 cgtacggctt ctcgtcggac gtgcgcggct cgctgtacgt cagcctgatc atatcgctgg
    11641 tggccatggt ggtgaccatg ctgttcgtct ccgtcatcgt gcggcgcgag cgggtgagcg
    11701 gcaaccgctc catcgtgctg ggatcggcca tcatgatgag cgcgggcagc gtgctcacgc
    11761 cgttctccga tcctaccacg cccgtcggca tgctggtgct gggcctgagc gcggtcatga
    11821 ccggcacggg ctcggccgtg ctgttcttgt gctggatcga gctcgagtcg gggctgggag
    11881 gccgcttggc gcttgtggag cttgcctcgt cgctgtgcat cgcgttcgtc gtggggttct
    11941 tgctgatcgt agggccggcg atacccgtca tcgtgctcat cgttgtcatg cccgtgcttt
    12001 cggcggcagc tctcaggcgc agcgcgcccg atacgccgca gctgccgcgc gagcccgagc
    12061 agcctttgtc gcgccatacg gcgaccctgt tcgcgaaggc gcttctgggc gcggtgctca
    12121 tcggcatgtt gatggggttc ttcgacgtgg tgtcggggtt caaaacctac gaggtgcagg
    12181 acatcttcgg cacgtacctg tttctggcgg gcttcatcgg agcgttggcc atctgcctga
    12241 tagccgtgtt cctgcagcgc gacagcgtgt tcttctcgta ccgcgtctcc atgctgatgc
    12301 tgtgcttggg atgcttgtcc acgccgttca tggccgacaa caacacgtat tcgagcgcgc
    12361 ttatcttcgg cggctaccac tgcttcgtgc tggtgctgtg cgtggtgtgc atcgacgtgg
    12421 cgtccagctt ccgcgtgagc ccggcgcgga cgatcggcct ggggttcgtg gcgctgtacg
    12481 gcggcgagac cgcaggccag ctgttcgccc attcgctgga ggcgaccggg gtcagcatgt
    12541 tcgacctcac gctggtcacg ctggtggccg tgtcgctgct gttcatcgcg catttgttcc
    12601 tgttcacgga gaccgacctc atcaaaatcg gcatcggcga ggtcagcctg atgggcacgg
    12661 tgcccgaaga ggccgccgac gacccggccg acccggtgga tccgtgcgcg ctgatcgcgg
    12721 agcgcttcgc cctgtcgccg cgcgagaccg acgtgcttcc gctgctgctg gagggccgca
    12781 ccatctcgcg catccaggag acgctgttca tctcggcggg cacggtgagc acgcatatcc
    12841 gccacatcta ccagaagacg ggcgtggaca atcgccagga gctcatcgac ctggcccacg
    12901 agacggccgc gcaggacgag gcggtctgag gcgatggaga cggcgtatct gcacgagttc
    12961 gcgaaggtgg ccgattgcgg cagcttcacg gccgccgcgc gcgagctgca tctgacgcag
    13021 tcgacgctga gcaagcacgt ggccttgttg gagcgcgaat tcggggccga cctgttcgtg
    13081 cgcgaccgca gcggcgtgag cctgaccgag gcgggcggcg tgctgtacgc ccaggcgcgg
    13141 cagatggaga agctgctgcg cgccaccgag ctgctggtgc gcgcggcgcg cgacgggcag
    13201 ggttcggcgg gcgcgggggt tgcgggcgcg acggcgggcg cagcggcggg cgggggt
//
