LOCUS       sequence-375970-       13257 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  E. coli - Eggerthella lenta shuttle plasmid (KanR), native 
            promoter-dopamine dehydroxylase from dopamine-metabolizing E. lenta 
            A2 strain with transcriptional regulators DadR/DadS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13257)
  AUTHORS   Dong X, Guthrie BGH, Alexander M, Noecker C, Ramirez L, Glasser NR, 
            Turnbaugh PJ, Balskus EP
  TITLE     Genetic manipulation of the human gut bacterium Eggerthella lenta 
            reveals a widespread family of transcriptional regulators.
  JOURNAL   Nat Commun. 2022 Dec 9;13(1):7624. doi: 10.1038/s41467-022-33576-3.
  PUBMED    36494336
REFERENCE   2  (bases 1 to 13257)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-12-9"; volume: "13(1):7624. doi"; pages: " 
            10.1038/s41467-022-33576-3"
COMMENT     Sequence Label: pXD70DA7-DadR/DadS-Pdadh-DadhABC(A2)
FEATURES             Location/Qualifiers
     source          1..13257
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2998..3792
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     terminator      complement(3915..3942)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     misc_feature    10964..10983
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     primer_bind     complement(11140..11158)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        11226..11330
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11331..11399,11400..12191)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11549..11568)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12362..12950
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12851..12870
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(13131..13202)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttatagcatg gcggcaaacg ctggcgagac gaaggtgaag ctctgggagg gcatctgcta
       61 cccagagaac atgcgcgatg actggcagga cgagatagac gacattcttc aagtgccttt
      121 cgcttatgcg gttcacgaca tagaccatga ccagaagagc aagcagcgca agacgcatgc
      181 gcacgtcatt gttgtctggg gcggcaacac gacacgcaag gcaatcatca acgtcttgaa
      241 ccgattgagc gcggacggca agaagtgctg ttcgtcagcc gagccaatca acaacatcag
      301 gcatgcgtat gactatctga ttcacgacac ggcttcctgt cgcaagaagg gcaaggagct
      361 gtatccggtc gaagcacgca tcgagggaaa caacttcgac attggtcagc tcgaacagct
      421 ctcgactaag gacaagcagg acatgctctt cgagttggtc ggcttcatca tgtgcgagag
      481 gttcgagacc atcaacgatt tcacggctgc ggcgctgagg gagtttcccg agcagtaccg
      541 cgaaatcatc gtggggtaca actcgattct cgagcgctat tgtcgcggga actatctgaa
      601 cgccgaacgt aagcgcaagt gcgtggaaca gtcaggtgcc aaagagtagt cgggtcaatg
      661 cagcaatgcg tgactacggg gcggcgtggt ctgcgctcgc gcagatctaa gccgactcgt
      721 tcacggattg cggaattgac gcgacggcca accgaataga ccagacttga ttaagggtcg
      781 atggacgctc tgctccatcg accctttcct tgcccgtgtc tcgactggat gagacctgca
      841 gaatcgggcg gctcattatt tttcgctaac tacgtccgaa aaataatgtg tccgaaaaat
      901 aatgtgacaa atgacaaatg acaaatgacc acaggggggc cactacgacg gcccccctaa
      961 tagtgccgtg tgccgtgtgc cgaaccgcag gtagattgag gggtggttcg gaggggcgaa
     1021 gtccctccgt tcggcgctcg cgccgatggc ccgagggccg tgagcgaagc cgcgaacggg
     1081 caagggctgg ggggtctcgg ggggaacgcc cgagcgggtg cagggctggc cctgccacgt
     1141 ccttagcgta gctaaggtct aacgtgctag actttagtcg attaagtctg tacggttcgg
     1201 aggtttccat ggctcacatc gcgaaataca aggctccatc ggtcgggcac atgcttgccc
     1261 actaccgccg cgaccgcagc agcctggagc gcgacaacat cgaccccgag cgcatcaaga
     1321 acgacttggt ggtcggccac tacacgaaca aggacggcaa gctcgttgtg gggcgcgtgg
     1381 agccgcgcga tggcgagccg aactggggca cggtggagag ccgcatcgag cgcgtgaacg
     1441 aggcccagaa ggccgcaggg aagcgtgcca cgcgcaagga tgcggtcgtt atggctgacg
     1501 ttgttgtgac gcttcccgac aatgtgcgca agggcgatga ggacaggttc ttccgtctca
     1561 cgtactggta cctgtcgaag aagttcggca tcgagaacat gatgggcggg tacgtccaca
     1621 aggacgaggt gttgaaggat ggcacgcccg cccgcgacca catgcacgtg ccgttcacgc
     1681 ccatcctaga tggacggttc aactacaagc agatgtgccc ccgcagcttc taccggagca
     1741 tgcacaagga gcttggggat tacctagagg ggcgcatggg ctaccgccct gcaatcgagc
     1801 ttgacgagga gacgcgggcg cagcgtgtat acacggacaa gtccgtggat atcgacaagg
     1861 tacgctgtgc ggtcgataag gccgtggttg aacctgcgaa ggacgaagcg gcgcgaatcg
     1921 tcgcggcggc tagggaagaa gccgccgctt tactgaatga tgccgaaacg cgtaaggcag
     1981 agcttgttac cgagattgcc gacaaggagg gtgaccttgc cgagcttgac agccagctcg
     2041 aggacgtgaa gctcgatatc gaagatgagc aagaccgatt ggagtgtctt cggcaacgag
     2101 cggacggagt tgcgcgagac attggagagc ttcaacccat cgctaccgag gttcggggct
     2161 gggaagctgc gggaaaagct gagcgcggcg caatcctcga taacatcgtt gttaagtgcg
     2221 atggactcgc gagccgcatc agagcaggag ttgcgggaat gcgaatcaaa gtggaagagc
     2281 ttagaagtcg aatatcgcgc ccgctcgaat atctgatgcg cagggaacag ccaaggcaac
     2341 agagccttaa tgacgtgctg cgcgatgcgc agcgggcggc ggatgcgtgg aaccgcgacc
     2401 acgctgcacc gcagaggacg tatcgcgggc gggctagatg atgtgttata gtagtacctg
     2461 taatacacga caaggaggtg cgacgcgaaa aaaccccgct gaagcggggt tttttgcgga
     2521 gcttcgcttc cacgatgcgc atgattcgga ggccccggcg atcgccgggg cctccgtgcg
     2581 ttgtgtgtcg atttgggatg cagccagatg ggcttcctcg gcgcgaaccg gccgagattt
     2641 ccgaatgcag acggtctcgc ttggacgatg cgctttcgaa taatcccggc ggagcgggca
     2701 ttccagtctt cttatcaata tatctataga atgggcaaag cataaaaact tgcatggact
     2761 aatgcttgaa acccaggaca ataaccttat agcttgtaaa ttctatcata attgtggttt
     2821 caaaatcggc tccgtcgata ctatgttata cgccaacttt gaaaacaact ttgaaaaagc
     2881 tgttttctgg tatttaaggt tttagaatgc aaggaacagt gaattggagt tcgtcttgtt
     2941 ataattagct tcttggggta tctttaaata ctgtagaaaa gaggaaggaa ataataaatg
     3001 gctaaaatga gaatatcacc ggaattgaaa aaactgatcg aaaaataccg ctgcgtaaaa
     3061 gatacggaag gaatgtctcc tgctaaggta tataagctgg tgggagaaaa tgaaaaccta
     3121 tatttaaaaa tgacggacag ccggtataaa gggaccacct atgatgtgga acgggaaaag
     3181 gacatgatgc tatggctgga aggaaagctg cctgttccaa aggtcctgca ctttgaacgg
     3241 catgatggct ggagcaatct gctcatgagt gaggccgatg gcgtcctttg ctcggaagag
     3301 tatgaagatg aacaaagccc tgaaaagatt atcgagctgt atgcggagtg catcaggctc
     3361 tttcactcca tcgacatatc ggattgtccc tatacgaata gcttagacag ccgcttagcc
     3421 gaattggatt acttactgaa taacgatctg gccgatgtgg attgcgaaaa ctgggaagaa
     3481 gacactccat ttaaagatcc gcgcgagctg tatgattttt taaagacgga aaagcccgaa
     3541 gaggaacttg tcttttccca cggcgacctg ggagacagca acatctttgt gaaagatggc
     3601 aaagtaagtg gctttattga tcttgggaga agcggcaggg cggacaagtg gtatgacatt
     3661 gccttctgcg tccggtcgat cagggaggat atcggggaag aacagtatgt cgagctattt
     3721 tttgacttac tggggatcaa gcctgattgg gagaaaataa aatattatat tttactggat
     3781 gaattgtttt agatcgttaa gggatcaact ttgggagaga gttcaaaatt gatccttttt
     3841 ttataacagg aattaaataa taaaaaagcc ggattaataa tctggctttt tatattctct
     3901 ctctagtata taaacgcaga aaggcccacc cgaaggtgag ccagtgtgag tcgactagtt
     3961 tagtacatca ggaccactgg gtagcccagc tggtagatca gcacgcgaaa catcacggag
     4021 gtggccagcg cgccgatggc ggcgacgacc atgagcgtct tggaggcgcc caccttcttc
     4081 ttcgccacga aggcgcaggc cgcagccacg atcacggaga cgatggagcc ccagaacatg
     4141 ggcgctcccg cgccggacac catgacggtc atcaggctgt cgacgtgcgt gggggccgtg
     4201 gtcatcgatg tcgggtcggc gtagtagccg aattgcgcga tcttcgccat ggcgcaggcg
     4261 atgacgaaca ccacgtcggc cacgatctgc acgagggccg cgatgaacgt cagctggatg
     4321 cccgtcgcct cgacggcctc gtccttcttc aggatggaga tcagccagac ggccgcggcg
     4381 cccaggaggc acgcgttgcc cagatagaac gccacgagcg ccaggcccca cgcgggacgg
     4441 gccggcatga ggtaggagtg cgcggtggcg accatcatga gcacggcaac cgcgagggtc
     4501 gcccaggcca gcgccttggg aacgggcttg ccgcctcgaa ggaccacgaa ccatgcgacg
     4561 atgacgatgg ccagcgccac gcatccgatg agctcctggg tgatgcccga cgtgatgtgc
     4621 ccgaagccgt tgaagatgcg ctcccagtgc tcgaggtgca ggaaggctcc gatgccgcct
     4681 accaccagcg agacggcgga cgtgatgagc gcggtcatct gcaggttcct gccctggccc
     4741 ttgagcgcca ggatggacat gccgcccagc gtgccgcagg tcaagcatac gaacagcgtg
     4801 aagatgacga gaggccactg cgtttccatt actcatcacc tccttcccag tgatggtcgc
     4861 gtaggatgta ggagtacgac ggcttgttgc ccacgtcggg cagcttgtgc acgtcggcgt
     4921 cggtgaacgg ctccaggaac tccaccatct tgcggcgctt gcccagcggg tcctcggagc
     4981 cctcgaacga ctcgtagccg gcctcggtgt cgccgatcca gcgggcgttg ccgccgcact
     5041 ggctgacgca ctggggcagc tcgccctgcg acagcttctg ctcgcacagc gtgcacttct
     5101 cgacgacctt ctcctcctcg ttgaggtagc ggacgccgta ggggcaggcc atgacgcaga
     5161 actggcagcc gatgcacttc tccttgtcga tctggatgga gccgtcctcg gccacgtgcg
     5221 acgcctcggt ggggcacacc ttcacgcact cggggttctc gcagtgctgg cagctgatgg
     5281 gcaggaagta catgtacacg tcggggtact gccctgatcc gcccgggatg gggttcggcc
     5341 ccacgcgcac cactttgttc cagtagctgc cgatgggcac gttgttgacc gccttgcagg
     5401 cgaccgagca cgccaggcag cccacgcacc ggttgaggtc ggtgatgata gccttcgtca
     5461 tggtttactc cctcccttcg taggtaggca gccattcctt cagacggggg tcgtcggagg
     5521 tatggatgat gggcgtgccg gtggagtcgc acggcacggg gttgttgaac ggcgagttct
     5581 cgggcgtggc cttgtagatc ttcacctggt aggcgcgcag gttggacgtg ccggagtgcg
     5641 ggtcctgggc gtagtggtcg atcagctggt tcacctggga gaactgccag ccgtggccgg
     5701 cgtccttcac ctcggggtac caccacgtgt gctcgaggtt gatgacgtct tccttcacgc
     5761 cgtagtacag gtcagccact tcgcggatct tgccccactc ggtttcgatc cacacccagt
     5821 cgccctgctc gatgccgtac tcggcggccg tcttcgggtt gatctccacg cgcggcacgg
     5881 gccacagctc gcggcaccag ggaagctggc gatgctcgga gtggaagtac accgggatgc
     5941 gacggccggt ggtggcggtc agcgggtact ccttgatgcg gtcgccgtcc ttcgggccgt
     6001 gaggcggctc ggtgtaggtg ggaagcctcc actcgggatg gtcgggatgg tgcgattcca
     6061 tgacggtgga ccagatctcc tgcttcatcg tcggggtgaa ccagcccggc ttccagtcgc
     6121 cgatgccctt gccggccgtg tagtcgaggc ggccccacac gcggtcgcgt gcgcgcatcg
     6181 cgccggtctg gtagcggcgg tacgtgcccc agttgcgcgg ctcgatgtcc ttcgcctgcc
     6241 accagccgtg ctcctggaag gccttgacgt actcgtccca cgtcttgtac ttgggcgtga
     6301 cctcggccat cggaacgccg tggcgctcga acgtgggctt gccgttctcg acgtgccacg
     6361 tggtgtaggt cagctcgtcg tcggtgagca gcttgatgga gtcgtccagc tgccagttga
     6421 tgtcgggcca ctcgttgccc ggctcgtcgg tccacttcca gttcatgcgg cgggcgaggt
     6481 ccatgacgat ctcgggatcg tacttggctt ccgcgggcgg ctgcacgcac ttgaccgtgg
     6541 cgcccatggc gccggcggaa ccttggctgg cgcgcggcga gttgagctcg atccaatggg
     6601 ccaccggcag gatgatgtcg gcggtgtcgt tctgcggcgt gtgccacagg ttgaggtcca
     6661 cgtagaagtc aagcgagcac agggcctccc aggcgcgcgt ggtgttcgtc tggctcatga
     6721 agttgccgga ctcgttccag agggcgcgca ccgggtaggg gtcgcccgtg atgacggcgt
     6781 cccacagcga atggctgtcg caccagtact gccaccagcc cagaagcggg aacttgtcga
     6841 tgccgatctg cttgcggttg acttcctccg gcggggtggt ggcgccgggg gcccaggcgc
     6901 tgaagccctg gaggtcgccg tcgatgggga cgatggtcgg gccgcggttg ccgcccgggg
     6961 tgtccatgtt gccggtgatg cccacgaggt tgtcgaaggc gcggcagttc tggatggcgc
     7021 tgcagaagtg ctcgaccgcc agcatgtact ggatgccgcc gttgccgtag ccggtggacg
     7081 ggtcgatgcg cgtgccgtag gccgtggccg ccgcctcgat ctcggaggcg gggatgccgg
     7141 tgatctcggc cgccacctcg ggcttgtact cggcggcgcg ggccttgtag tgctcccaca
     7201 ccggcttgac cttcacggtc ttgccgtcct tcagcgtgat ctcgaactcg ccttccagcg
     7261 cggggtcgat ggcggggtcg aacggcatgg gatcgggcag ccagccctgc gtctggcccg
     7321 gcagcaggtt gtcctgcgcg gcctcgcggc cttggcgcgc cttctcggag tcgtagttct
     7381 cgccctccca gaagccgccg gtctcgtcga tgacgccttc ctggtcggcg ttgaaccagg
     7441 tgaacgcgcc gtactcgtgc tcgacgccct cggccttcag cttctcccag ttgttgtcgt
     7501 acacgaggaa cttatagggg ctgccgccct ccttgatgtc gctttccttg agcagcgcgg
     7561 ttttcacgtc ccagtaggaa ccgtcggtgc gcacggtggg gaagccggag ggctccatgt
     7621 cctcgcacac gaggaacggg gcgttcgtcc acttcttcac gtacagctcg tcgtagagct
     7681 tcttctcgat gatgacgttc gtccaggcca gggccagggc gccgtcggtg ccggggcgca
     7741 ggtgctgcca gtagtcggct tccttgccca tgttggccat gcgggggtcg acgctgatgt
     7801 gcacgtcggc gcgcgatgcc acgtccaccg tggtgcggca ggagtcgtcg tagttggaca
     7861 gctcggacgc gccgccccac tgcacgtaca ctttcgggcg cgtgatggtt tccatccacg
     7921 acatggcgaa ctgcgacacc atggtggtgg cgaagtggcg cgggcccttg cagatctgcc
     7981 aggcctcaac gttgttcggc gtttccagca tgttcttcag gatggactcc gagtgcatgc
     8041 accagatgcg cgacgtgccc acctggcagg cgatggcctc gccgccgtgc ttcgccttga
     8101 tgtccatgaa gttgtccacg atggactgca tggcctcgtc ccacgagatg cgctgccagc
     8161 cgggctcctc gcccttcggg ttcgtgcgct tcatgggata gtgcagacgg tcggggtggt
     8221 acgccgcctg gatggacgac tgcgacttcg tgcagcagtt gcccatggac tggaacgctc
     8281 cctcgtcgcc ctcgacgcgt atggcccgcc cgttctggac gatgaccttg acgccgcact
     8341 ccatcttgcc gcagccgcgg cagcacgttt tcacggccac ggtgtcgttg ccggacacgc
     8401 tgctgtacgt ctcttcggcc agcgcggtgt gcgtcgaagc gccgaacgcc gcggctgccg
     8461 caccggtgat ggcggcggtc ttgacgaacg tgcgtcgtga catggtcagg ttacccatac
     8521 tcctcctcct tctttctcct cgttctcgtc ttgctcgtct tcgttcgtgc gatcggggag
     8581 gggggctgcc gggtgaagcc tcgggctccg aaccggtgca aagtccccgt gtcgcttggg
     8641 ccgaccatac ggatgcgagg gggtgcagag catcgtccaa aggttggaaa gcaggttcga
     8701 ccgcatcata aattcttatg atcttgtgat catgaaaaat tcgatagtgc tttgaactgg
     8761 ggaaacttta accgtaggtc ataaattatt ctgaaactag aatagtttcc tcaaagaggg
     8821 ccgctcatct tttccgtata atcgaacccg tcgacagagg ggtcggcgcg agcgacgtta
     8881 tgtacgggct cacagggggt ggggcatgga aaagcttcgg agtgcatggg ataccgcgtt
     8941 ggggacgagc ctttccctca agcgcatcgc gggcgcggcc ttgcaatggc tttggatgct
     9001 catgatgttc tacagcatcg tgccgtacgg cttctcgtcg gacgtgcgcg gctcgctgta
     9061 cgtcagcctg atcatatcgc tggtggccat ggtggtgacc atgctgttcg tctccgtcat
     9121 cgtgcggcgc gagcgggtga gcggcaaccg ctccatcgtg ctgggatcgg ccatcatgat
     9181 gagcgcgggc agcgtgctca cgccgttctc cgatcctacc acgcccgtcg gcatgctggt
     9241 gctgggcctg agcgcggtca tgaccggcac gggctcggcc gtgctgttct tgtgctggat
     9301 cgagctcgag tcggggctgg gaggccgctt ggcgcttgtg gagcttgcct cgtcgctgtg
     9361 catcgcgttc gtcgtggggt tcttgctgat cgtagggccg gcgatacccg tcatcgtgct
     9421 catcgttgtc atgcccgtgc tttcggcggc agctctcagg cgcagcgcgc ccgatacgcc
     9481 gcagctgccg cgcgagcccg agcagccttt gtcgcgccat acggcgaccc tgttcgcgaa
     9541 ggcgcttctg ggcgcggtgc tcatcggcat gttgatgggg ttcttcgacg tggtgtcggg
     9601 gttcaaaacc tacgaggtgc aggacatctt cggcacgtac ctgtttctgg cgggcttcat
     9661 cggagcgttg gccatctgcc tgatagccgt gttcctgcag cgcgacagcg tgttcttctc
     9721 gtaccgcgtc tccatgctga tgctgtgctt gggatgcttg tccacgccgt tcatggccga
     9781 caacaacacg tattcgagcg cgcttatctt cggcggctac cactgcttcg tgctggtgct
     9841 gtgcgtggtg tgcatcgacg tggcgtccag cttccgcgtg agcccggcgc ggacgatcgg
     9901 cctggggttc gtggcgctgt acggcggcga gaccgcaggc cagctgttcg cccattcgct
     9961 ggaggcgacc ggggtcagca tgttcgacct cacgctggtc acgctggtgg ccgtgtcgct
    10021 gctgttcatc gcgcatttgt tcctgttcac ggagaccgac ctcatcaaaa tcggcatcgg
    10081 cgaggtcagc ctgatgggca cggtgcccga agaggccgcc gacgacccgg ccgacccggt
    10141 ggatccgtgc gcgctgatcg cggagcgctt cgccctgtcg ccgcgcgaga ccgacgtgct
    10201 tccgctgctg ctggagggcc gcaccatctc gcgcatccag gagacgctgt tcatctcggc
    10261 gggcacggtg agcacgcata tccgccacat ctaccagaag acgggcgtgg acaatcgcca
    10321 ggagctcatc gacctggccc acgagacggc cgcgcaggac gaggcggtct gaggcgatgg
    10381 agacggcgta tctgcacgag ttcgcgaagg tggccgattg cggcagcttc acggccgccg
    10441 cgcgcgagct gcatctgacg cagtcgacgc tgagcaagca cgtggccttg ttggagcgcg
    10501 aattcggggc cgacctgttc gtgcgcgacc gcagcggcgt gagcctgacc gaggcgggcg
    10561 gcgtgctgta cgcccaggcg cggcagatgg agaagctgct gcgcgccacc gagctgctgg
    10621 tgcgcgcggc gcgcgacggg cagggttcgg cgggcgcggg ggttgcgggc gcgacggcgg
    10681 gcgcagcggc gggcgggggt gccgtcgacg gctcgcctgc ggccgcgtgc gacacggcgc
    10741 ttcgctgcaa gtgccggctc gcggccgagc gctgcgggct cgaccggcgc gagctgggcg
    10801 cgctcatcct gtacgtggag gagcgcggct tcgaggcgat ccagggcgag ctggcgctga
    10861 cgcgcgacga ggtggccgac gtgctgggca gcgtgtaccg caagctgggc gtgggcgaca
    10921 agcaggaggc gctcgatttc atccattccg tttcggaatg aatctagaag cggccgcgaa
    10981 ttcagaatag taatacagga tccgaatcgt ttcagttgcc gccggattaa taatcaaata
    11041 accgttccta aaaaatgccc tcttgggtta tcaagagggt cattatattt cgcaaaaaac
    11101 cccgcttcgg cggggttttt tcgccttgaa gacgaaaggg cctcgtgata cgcctatttt
    11161 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
    11221 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
    11281 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
    11341 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
    11401 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
    11461 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
    11521 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg
    11581 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
    11641 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
    11701 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
    11761 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
    11821 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa
    11881 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
    11941 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
    12001 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
    12061 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
    12121 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
    12181 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
    12241 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
    12301 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
    12361 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
    12421 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
    12481 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
    12541 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
    12601 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
    12661 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
    12721 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
    12781 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
    12841 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
    12901 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
    12961 acgcggcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc
    13021 tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt
    13081 ggagtggtga atccgttagc gaggtgccgc cggcatgagc tcctctagta gagagcgttc
    13141 accgacaaac aacagataaa acgaaaggcc cagtctttcg actgagcctt tcgttttatt
    13201 tgaaaaagaa aagccccagc aagcttggcg gcagcagggg cattcgagac aaggaga
//
