LOCUS       sequence-375975-        9112 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  eat-4(prom6-1) fluorescent neural reporter driving nuclear 
            mNeptune2.5 expression (refer to NeuroPAL paper for expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9112)
  AUTHORS   Yemini E, Lin A, Nejatbakhsh A, Varol E, Sun R, Mena GE, Samuel ADT,
            Paninski L, Venkatachalam V, Hobert O
  TITLE     NeuroPAL: A Multicolor Atlas for Whole-Brain Neuronal Identification
            in C. elegans.
  JOURNAL   Cell. 2021 Jan 7;184(1):272-288.e11. doi: 
            10.1016/j.cell.2020.12.012. Epub 2020 Dec 29.
  PUBMED    33378642
REFERENCE   2  (bases 1 to 9112)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.012"; journalName: "Cell"; date: "2021-01-7-
            7"; volume: "184"; issue: "1"; pages: "272-288.e11"
COMMENT     Sequence Label: pEY43
FEATURES             Location/Qualifiers
     source          1..9112
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6..22
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             4619..4636
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             4787..4807
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4832..4852
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4874..4894
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(6703..6725)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6825..6844
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6922..6940)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7008..7112
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7113..7181,7182..7973)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7331..7350)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8144..8732
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8633..8652
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    9020..9041
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9056..9061,9062..9079,9080..9086)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9094..9110
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(9099..9112,1..9)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcacactgg ttgatgatga
      181 tgatgatatg agggatatct agtaaaagag gatgcgctaa tccgatcgtt gtgcttaaat
      241 ctgagcaaag tggaggaaag aagagagata gaaaaagagc catcaacact cattttcact
      301 ttttttgaca ttcatcataa tccctgatac tggtggactt ctttcggata ttgtttttta
      361 catttatttc gaatttgtca ttttttataa gcccaggttt gcattttttt ttaaagttat
      421 tacagctgga attttgggat tttcctgttt tcaactgaag taatgtactg aaaatagcaa
      481 aattatttct tcaactaact gtaacttttt gaaactctgc ataaaaacag tagcttttga
      541 aatatagact ttaaaacttt cgaataaatt tttctaattt tccactcaca atactcaaac
      601 tataatactc aaacttataa tactcaaact aacaaacctc aaaaaattaa tgaagtactt
      661 aaacgaaacc tgtaacttta agaagttcat gcaactggct ttctgagatc acagaaattc
      721 tgccaatttg gattctaaat ctgaaaattt tgcatctgaa aacttggcac caatcattta
      781 cgattccata tctcccttga aaaagctata tgaaaacttg atctgggttc agtacacaat
      841 cttcagactc ttcagtccag gcctaaagac caaacgggca aattttttgg ttttgagaag
      901 ctgaagcgct gaagaaagtg tactattttc gtcaaggcta caatccgtct agtggttcat
      961 tgccaaacaa agttttaaaa atctaaaaac tatcaatcct aatgttcttt ttctaacatt
     1021 cagccagaga aacgcttcac actctgtatt ttccaaaatt tattgcagaa ttgaatgttt
     1081 attacatgtt tagttgatat cacttgtcta ggagaatata ttctagctat tcaattagtt
     1141 tggatatatt atgactgaaa actcgggcca agttttacaa actcatagat caatataaaa
     1201 tatccggatg caacctgacc attaatagaa aacaatatta gagataactg tgtggtgttc
     1261 atttgttgat atgcttttcg tacatatgta cttttcatat acctcttgca aattgttatg
     1321 catctaatca cctttatcca tgaagaaagc gatattcttg gcaagaaaag ttttgtgctt
     1381 cctcccgact gcgtgctaaa agccaaataa attatcgcgc cgaggactcg agactgactg
     1441 aataatttta tttatgtgtt tcccttctcc tgacgtgtcg caattacttt ttccctgtgt
     1501 gtgtgtgtgt gtgaggtgaa aggcgtcgcc gtcgagtgtg tgtgtgtgtg tgctcggaga
     1561 gccgtaattc aaaacttgtt gaaaaagctg tgtgtggggg cacaagtcac tagaaaaatt
     1621 gagaggttga aaggtttttg agagaacaac cgaaaaaggt gacgatttat gagttggaaa
     1681 ggtctggggt gccatccgtc tatttccaga atgaagtaga tctgacaaag ggataaactt
     1741 gaagtttgtc attttgacaa aatatcaacc atatttgata aattagaatt caaggttttg
     1801 aaaatttggt taaagcgttc gattctaaac caaaggtcat cagtaaaaat tattttttat
     1861 ccaatctata ttctatggtt gaggtgaaaa gttcgtcagt aggaatcttg tataaaaccg
     1921 ctcctatata gtgctaaaat acccaatcaa agaaacactt tttggaaacg tttttttgaa
     1981 agtttttttt aagtgaaagt cgaccaaaaa tttttttcat gatctctttt ttctatttaa
     2041 cctaggagtt cctaggagtt cgaaactgca gcaatgatcg aactttctca aaattccaga
     2101 tttttgaaaa acttgccagt ttaccaaatc gttgactgga agttttgaga aatccaaaat
     2161 tttgagctgt tttttttagt gatatttagt gatgaccgta tttaaaaact gaaactttaa
     2221 aaaaacatca attttctaag ttcatccctc actaaagaga cgcagatatg gtgattcgaa
     2281 gagcctacaa aactactacc aacaacacat atttcccgtt taatcaacta atgagatttt
     2341 catgttcttt tcattgtcgc gcattgactg cttacacctt tttcttatgt ttgtttatgt
     2401 tcatgtaagc aaaaagaatg aacaagtcgg tgacttcaga tgactacgtt cttccgaatc
     2461 ctcggagctc agtttgaaat tgctgctcta tgcttacaac cttgctacat atattacaga
     2521 attttagatt caaaacttaa atcttaaaag ttgaatagtt aattgagcaa tatatttttt
     2581 ttggaatttt gagaacactt aaaactagaa cccaaaattt ctcaacaatt ttttatggcg
     2641 attcaaaatt tctaataaaa cggtctacca ttttgagtct attatagccg aaaatctcca
     2701 atgtgactgt gacttcttaa aactactaaa acattatttg tccatttaca tcttcctaaa
     2761 accgtatatc atcaaaaaca ttcacaaaat ccgaaaaatg agacaaaaat ttttttttga
     2821 ttgttattgc aataaatcta ataaaaatat tcatatattg cctggcgccc cccatatctc
     2881 catttccggt cccatcaccc ccacacctcc aagattgata ggtggctata agcatttttt
     2941 gcatttgaat gtgttgcacc agtagtcatc atcatcatta tctaaactga cgtgatagta
     3001 gggggctttc tagaagtcga ttttctatta atgtcaactt cattcgttgt cccttccttt
     3061 ccccgtcttc cctcacttcc ttttttctat tttttccagt ggtccgtagt gggcggcacc
     3121 cgattttgac ttgaaatcag acccgtttcc ggttcttttg gtagttgtta agttctgatt
     3181 ctatgacgtg gagtgaaaca aagaacgacc atatttcatg tgttgtgttt tctaggcagt
     3241 ctaggcaggc agtaggcaga tactgtcaaa attggaatat ttccatcttc taaataccct
     3301 caactatttg ttagcgcgtt tgaaacaata attgcaaaac atttttttcg cattgattgg
     3361 ggcattttga aatttagagt aaaatcgact atcaactgtc attccataat aattggcaga
     3421 attattttgg ttatgccacc aaataatcaa taaagaaaga tttctgtcct tattaaacta
     3481 aaaattgaag caacggtaga gttgctgaac acagtcgccg gcaaaatttt ttaattttct
     3541 gcaaatttga aattcttttg cgggttttta gttatggttc taatagtggt taaaagtcat
     3601 tataaaacac ttcaattttt tgtaatgctt tcattcgcag tcgtgaagtc gtgaaaactc
     3661 agttttcacc tatcctaact ttggaagtcg tccaaaaaat tattttaatt ctacaatttt
     3721 atatttcttt ttaaacatac gatgtgatca atcctacatc aattctgcaa atttctcaca
     3781 ttcttggaag cttggtaatt tatcagactt tgactgaaaa tttgaaaaaa aattcagtaa
     3841 tttttggaga tccctttaaa tattattcta gcattgccat atagaataat tgcaaaattc
     3901 aattggtttc ctagaaagaa aattagataa tcttatgaaa gagaacctaa ccacaacagg
     3961 tgttaaatat tgatttaaac actaaaaaaa gtctctcctt ctacctctct tctcatagct
     4021 ttcatgtctt gaagcttttc ggttaattcg agcggaagac ttatcaaggt acgtcatttt
     4081 caattacttg tatacatctc tcagcttctt ctaattttcc atgtacatct tagatattct
     4141 tttttcagcg acttgatatt aggaagtttt gtgttttcaa ttttaaagtt ggattaatat
     4201 agaataccag tctttaaaca caaaccaaca agggttcaat atcaaaatag aaaccgaaaa
     4261 aaaatatcga aaactgaaaa aatcaaatac atatctaaag caagctattc gagaaatatt
     4321 tatgcattat aacaactatg cagcgcttat atctttattt ttcaacaagt gttccagcaa
     4381 cgagagtctc ttcaccaaaa agccatctat caaaaaccag gcagtgagtc ctagaaccaa
     4441 gcttgtcaga agacaagtgc atgtataaat aagtagtaaa agacgggtgg gacccagcgc
     4501 ggtgagtagt agatcataaa aaagtgactg aaaagaaggg gcgtttcctt ttctttatta
     4561 cctcttcctc tacttcttct catctcaact tgctttttcc ttcctcttac aacaactcca
     4621 tcatcatcat catcattttc agaaaccacc cgggattggc caaaggaccc aaaggtatgt
     4681 ttcgaatgat actaacataa catagaacat tttcaggagg acccttggag ggtaccggtt
     4741 ttcccagtca cgacgcgtgg taccgagctc agaaaaaatg accgctccaa agaagaaacg
     4801 caaagtaccg agctcagaaa aaatgacggc acctaaaaag aaacgcaaag taccggtcgc
     4861 cgagaagaca gccccaaaaa agaagcgtaa agttccacgc gtcatgggag taagtttaaa
     4921 cagttcggta ctaactaacc atacatattt aaattttcag cgcgctgtct ccaagggaga
     4981 ggagctcatc aaggagaaca tgcacaccaa gctctacatg gagggaaccg tcaacaacca
     5041 ccacttcaag tgcacccacg aaggggaggg caagccctac gagggcaccc agaccaacag
     5101 gattaaggtg gtggagggag gccccctgcc gttcgcattc gacatcctgg ccacctgctt
     5161 tatgtacggg agcaagacct tcatcaacca cacccaagga atcccagact tcttcaagca
     5221 atccttcgta agtttaaaca tgattttact aactaactaa tctgatttaa attttcagcc
     5281 tgagggcttc acatgggaga gagtcaccac atacgaagac gggggcgtgc ttaccgttac
     5341 ccaggacacc agcctccagg acggctgctt gatctacaac gtcaagctca gaggggtgaa
     5401 cttcccatcc aacggccctg tgatgcagaa gaaaacactc ggctgggagg ccagtaccga
     5461 gaccctgtac cccgctgacg gcggcctgga aggcagatgc gacatggccc tgaagctcgt
     5521 gggcgggggc cacctgcact gcaacctgaa gaccacatac agatccaaga aacccgctaa
     5581 gaacctcaag atgccaggag taagtttaaa catatatata ctaactaacc ctgattattt
     5641 aaattttcag gtctactttg tggaccgcag actggaaaga atcaaggagg ccgacaatga
     5701 gacctacgtc gagcagcacg aggtggctgt ggccagatac tgcgacctcc ctagcaaact
     5761 ggggcacaaa cttaatggca tggacgagct gtacaagtaa gcgccggtcg ctaccattac
     5821 caacttgtct ggtgtcaaaa ataatagggg ccgctgtcat cagagtaagt ttaaactgag
     5881 ttctactaac taacgagtaa tatttaaatt ttcagcatct cgcgcccgtg cctctgactt
     5941 ctaagtccaa ttactcttca acatccctac atgctctttc tccctgtgct cccaccccct
     6001 atttttgtta ttatcaaaaa acttctctta atttctttgt tttttagctt cttttaagtc
     6061 acctctaaca atgaaattgt gtagattcaa aaatagaatt aattcgtaat aaaaagtcga
     6121 aaaaaattgt gctccctccc cccattaata ataattctat cccaaaatct acacaatgtt
     6181 ctgtgtacac ttcttatgtt ttttacttct gataaatttt tttgaaacat catagaaaaa
     6241 accgcacaca aaatacctta tcatatgtta cgtttcagtt tatgaccgca atttttattt
     6301 cttcgcacgt ctgggcctct catgacgtca aatcatgctc atcgtgaaaa agttttggag
     6361 tatttttgga atttttcaat caagtgaaag tttatgaaat taattttcct gcttttgctt
     6421 tttggggttt cccctattgt ttgtcaagat ttcgaggacg gcgtttttct tgctaaaatc
     6481 acaagtattg atgagcacga tgcaagaaag atcggaagaa ggtttgggtt tgaggctcag
     6541 tggaaggtga gtagaagttg ataatttgaa agtggagtag tgtctatggg gtttttgcct
     6601 taaatgacag aatacattcc caatatacca aacataactg tttcctacta gtcggccgta
     6661 cgggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
     6721 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
     6781 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat
     6841 tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata
     6901 ccgcatcagg cggccttaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
     6961 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     7021 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     7081 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     7141 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     7201 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     7261 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     7321 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     7381 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     7441 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     7501 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     7561 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     7621 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     7681 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     7741 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     7801 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     7861 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     7921 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     7981 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     8041 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     8101 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     8161 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     8221 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     8281 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     8341 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     8401 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     8461 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     8521 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     8581 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     8641 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     8701 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     8761 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     8821 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     8881 agcgcagcga gtcagtgagc gatgaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     8941 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
     9001 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
     9061 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tt
//
