LOCUS       sequence-375978-        7788 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  odr-1 fluorescent neural reporter driving nuclear mNeptune2.5 and 
            mTagBFP2 expression (refer to NeuroPAL paper for expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7788)
  AUTHORS   Yemini E, Lin A, Nejatbakhsh A, Varol E, Sun R, Mena GE, Samuel ADT,
            Paninski L, Venkatachalam V, Hobert O
  TITLE     NeuroPAL: A Multicolor Atlas for Whole-Brain Neuronal Identification
            in C. elegans.
  JOURNAL   Cell. 2021 Jan 7;184(1):272-288.e11. doi: 
            10.1016/j.cell.2020.12.012. Epub 2020 Dec 29.
  PUBMED    33378642
REFERENCE   2  (bases 1 to 7788)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.012"; journalName: "Cell"; date: "2021-01-7-
            7"; volume: "184"; issue: "1"; pages: "272-288.e11"
COMMENT     Sequence Label: pEY52
FEATURES             Location/Qualifiers
     source          1..7788
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6..22
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2079..2099
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2124..2144
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2166..2186
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3090..3143
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3153..3173
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3195..3215
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(5379..5401)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5501..5520
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(5598..5616)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5684..5788
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5789..5857,5858..6649)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6007..6026)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6820..7408
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7309..7328
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7562..7579
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7696..7717
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7732..7737,7738..7755,7756..7762)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7770..7786
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(7775..7788,1..9)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcctgcagc aagcagcaca
      181 accagacgcc cgatcttcga ataatttata aaacatataa ttatgggtac aacaatttct
      241 cattcaaatt tttacaataa ccaactttct ttcttccttt ccgtttccct tgtttttttt
      301 cttattttct cctggaaaac atactgttgt tcaattcata ttttgctatt tctcgcttca
      361 aacttgtgaa accgtattcc acacttgcca tcaggctata ttttgcaatg aacattttat
      421 ttggtggctc gagtgctatt tgtaaaatag aaaacagaac ggaaattgaa gcaattcttc
      481 catcaatctt tttttttata tattggagat gaatcgaaaa tttttgaatg atatatggaa
      541 agtctgatac atagggaaaa aaagaaatta aattgaaccc aactagagag atcaaacgat
      601 ccatctataa aaaacggaaa ccgtgagaaa tacgctattg gaataaaaag ggcttgtgtg
      661 ctctttatga atgcgtgaat aaggtaactc aatgactttc aaaacaaata aataaaacct
      721 tgaaaaattg cgtcaaaaat gtgcagaaaa acgatgaaag gggtataaga aatatagggg
      781 aaaatgtaac taataatgat tattgtatta cctcaaatag tattgaatgc aaaaataatg
      841 gttttcatgt ttactttgcg ctaaaaatga cgaatttgct aaagtctcga ctaaatattt
      901 ctcgttaggc gagaagtcag tgctgaccta atagaggctg caagactaat tttcggttga
      961 cttgcaaggg gggcaaaact aatattgaaa gacggtagtt agactttcaa ttgaatgaat
     1021 ttcacaccgt gtgcagaaat gataaaacag tatcaacata tataagtata atttgtttac
     1081 tttttgctaa acaatattac atccagtaat atgaaaaaat gatcaacgcg taaaacttcc
     1141 gtactttaca agaaacaggg caagtgaaaa aaaaacattt ctgttaccac atgtttccaa
     1201 caattgaagc aattcttgta atagataaca atacaaaatg taatcagaaa ttcagtggga
     1261 gtatccgctc cggaagtagt tgattggcca gcattatacc caaaatttat gagctaaatg
     1321 tattgtgtat tcgtaacatc atatttcttg cgagttttaa tttgattatc agaataacgg
     1381 taagtaaaat atgcttcatt gagctgaaaa ttaaattcaa aaaacacatg cgctcaaaca
     1441 actaattcta tctgaataca ttaagcagtt tcctgatcaa ttgttttgca tttatcaaca
     1501 tactttattt caaaaaaagt tagattcaaa ttttttttca gataacaatt tcgagaacct
     1561 gccttcatac agtaactatt caataaaatt ttatagtttg tcatattaat ttgtaacaag
     1621 tacagttatc taatttactt tgaactactt gattttatag ttaaggaagg taaaaaatcc
     1681 tactatacta atccatgtat tcattgcaaa gcccactaga ttcgtaatcc gttttgagtc
     1741 agctattcaa ctggaacaat atcatatcta ttctatgatt aaatacctat tcatttcaaa
     1801 atcttctccc ccacctattc actcttgcca tatttgatat atactttatc gatcttttca
     1861 tatcccttgt gagcaccctt gttttcatgc ttgaactcac aagttagctc attactgatt
     1921 attatcacct gaaaccctca cccgggattg gccaaaggac ccaaaggtat gtttcgaatg
     1981 atactaacat aacatagaac attttcagga ggacccttgg agggtaccgg ttttcccagt
     2041 cacgacgcgt ggtaccgagc tcagaaaaaa tgaccgctcc aaagaagaaa cgcaaagtac
     2101 cgagctcaga aaaaatgacg gcacctaaaa agaaacgcaa agtaccggtc gccgagaaga
     2161 cagccccaaa aaagaagcgt aaagttccac gcgtcatggg agtaagttta aacagttcgg
     2221 tactaactaa ccatacatat ttaaattttc agcgcgctgt ctccaaggga gaggagctca
     2281 tcaaggagaa catgcacacc aagctctaca tggagggaac cgtcaacaac caccacttca
     2341 agtgcaccca cgaaggggag ggcaagccct acgagggcac ccagaccaac aggattaagg
     2401 tggtggaggg aggccccctg ccgttcgcat tcgacatcct ggccacctgc tttatgtacg
     2461 ggagcaagac cttcatcaac cacacccaag gaatcccaga cttcttcaag caatccttcg
     2521 taagtttaaa catgatttta ctaactaact aatctgattt aaattttcag cctgagggct
     2581 tcacatggga gagagtcacc acatacgaag acgggggcgt gcttaccgtt acccaggaca
     2641 ccagcctcca ggacggctgc ttgatctaca acgtcaagct cagaggggtg aacttcccat
     2701 ccaacggccc tgtgatgcag aagaaaacac tcggctggga ggccagtacc gagaccctgt
     2761 accccgctga cggcggcctg gaaggcagat gcgacatggc cctgaagctc gtgggcgggg
     2821 gccacctgca ctgcaacctg aagaccacat acagatccaa gaaacccgct aagaacctca
     2881 agatgccagg agtaagttta aacatatata tactaactaa ccctgattat ttaaattttc
     2941 aggtctactt tgtggaccgc agactggaaa gaatcaagga ggccgacaat gagacctacg
     3001 tcgagcagca cgaggtggct gtggccagat actgcgacct ccctagcaaa ctggggcaca
     3061 aacttaatgg catggacgag ctgtacaagg agggacgtgg atccctcctc acctgcggag
     3121 acgtcgagga gaacccagga ccaatgaccg ctccaaagaa gaagcgcaag gttccagtcg
     3181 agaagatgac tgccccaaaa aagaaacgta aggtcccgcg gatgtcagag cttattaagg
     3241 agaatatgca tatgaaactg tacatggaag gaaccgtgga caaccatcac tttaagtgca
     3301 cttcggaggg ggaaggcaaa ccgtacgagg gcacccagac catgcgaatc aaggtggtcg
     3361 agggaggccc actgcccttc gccttcgaca ttctggtaag tttaaacata tatatactaa
     3421 ctaaccctga ttatttaaat tttcaggcta ctagcttcct gtacggcagc aagactttta
     3481 ttaatcacac ccagggcatc ccggacttct tcaaacagtc gttccctgag ggcttcacat
     3541 gggagcgtgt gaccacatac gaagacggtg gcgttctcac cgctacccag gacaccagcc
     3601 tccaggacgg ttgcctcata tataatgtgg taagtttaaa cagttcggta ctaactaacc
     3661 atacatattt aaattttcag aagatccggg gagtaaactt cacatccaat ggtccggtta
     3721 tgcagaagaa aacactcggc tgggaggcct tcaccgagac gctgtatccg gctgacggag
     3781 gcctggaagg tcgaaacgac atggccctga agctcgtcgg cggtagccat ctcatcgcaa
     3841 acgctaagac cacatataga tccaagaaac cggtaagttt aaacatgatt ttactaacta
     3901 actaatctga tttaaatttt caggctaaga accttaaaat gccgggagtc tactatgttg
     3961 actacagact ggaacggatc aaggaggcca acaacgagac ctacgtcgag cagcacgagg
     4021 ttgcagttgc cagatactgc gacctcccga gcaaactggg tcacaagctt aatcctagga
     4081 taccggtggg tgaagaccca ccaaagccat ctgccaaggg agccaagaag gccgccaaga
     4141 ccgttacgaa gccaaaggac ggaaagaaga gacgtcatgc ccgtaaggaa tcatactccg
     4201 tctacatcta ccgtgtcctc aagcaagttc atccagacac tggagtttcc tccaaagcca
     4261 tgtctatcat gaactctttt gtcaacgatg tcttcgagcg tattgctgct gaagcatccc
     4321 gtcttgctca ctacaacaag cgttccacaa tctcatcccg cgaaattcag accgctgtcc
     4381 gtctgatcct tccaggagag cttgccaagc acgccgtgtc tgagggaacc aaggccgtta
     4441 ccaagtacac ttccagcaag taggaattcc aactgagcgc cggtcgctac cattaccaac
     4501 ttgtctggtg tcaaaaataa taggggccgc tgtcatcaga gtaagtttaa actgagttct
     4561 actaactaac gagtaatatt taaattttca gcatctcgcg cccgtgcctc tgacttctaa
     4621 gtccaattac tcttcaacat ccctacatgc tctttctccc tgtgctccca ccccctattt
     4681 ttgttattat caaaaaactt ctcttaattt ctttgttttt tagcttcttt taagtcacct
     4741 ctaacaatga aattgtgtag attcaaaaat agaattaatt cgtaataaaa agtcgaaaaa
     4801 aattgtgctc cctcccccca ttaataataa ttctatccca aaatctacac aatgttctgt
     4861 gtacacttct tatgtttttt acttctgata aatttttttg aaacatcata gaaaaaaccg
     4921 cacacaaaat accttatcat atgttacgtt tcagtttatg accgcaattt ttatttcttc
     4981 gcacgtctgg gcctctcatg acgtcaaatc atgctcatcg tgaaaaagtt ttggagtatt
     5041 tttggaattt ttcaatcaag tgaaagttta tgaaattaat tttcctgctt ttgctttttg
     5101 gggtttcccc tattgtttgt caagatttcg aggacggcgt ttttcttgct aaaatcacaa
     5161 gtattgatga gcacgatgca agaaagatcg gaagaaggtt tgggtttgag gctcagtgga
     5221 aggtgagtag aagttgataa tttgaaagtg gagtagtgtc tatggggttt ttgccttaaa
     5281 tgacagaata cattcccaat ataccaaaca taactgtttc ctactagtcg gccgtacggg
     5341 ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg
     5401 gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg
     5461 tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta
     5521 ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc
     5581 atcaggcggc cttaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
     5641 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     5701 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     5761 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     5821 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     5881 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     5941 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     6001 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     6061 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     6121 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     6181 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     6241 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     6301 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     6361 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     6421 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     6481 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     6541 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     6601 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     6661 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     6721 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     6781 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     6841 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     6901 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     6961 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     7021 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     7081 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     7141 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     7201 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     7261 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     7321 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     7381 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     7441 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     7501 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     7561 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc
     7621 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
     7681 tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt
     7741 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatt
//
