LOCUS       sequence-375981-        9132 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  E. coli - Eggerthella lenta shuttle plasmid (TetR), cadR 
            complementation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9132)
  AUTHORS   Dong X, Guthrie BGH, Alexander M, Noecker C, Ramirez L, Glasser NR, 
            Turnbaugh PJ, Balskus EP
  TITLE     Genetic manipulation of the human gut bacterium Eggerthella lenta 
            reveals a widespread family of transcriptional regulators.
  JOURNAL   Nat Commun. 2022 Dec 9;13(1):7624. doi: 10.1038/s41467-022-33576-3.
  PUBMED    36494336
REFERENCE   2  (bases 1 to 9132)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-12-9"; volume: "13(1):7624. doi"; pages: " 
            10.1038/s41467-022-33576-3"
COMMENT     Sequence Label: pXD70Tet(CadR)-CadR
FEATURES             Location/Qualifiers
     source          1..9132
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(4967..4994)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     misc_feature    6834..6853
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     primer_bind     complement(7010..7028)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7096..7200
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7201..7269,7270..8061)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7419..7438)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8232..8820
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8721..8740
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(9001..9072)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gcatggcggc aaacgctggc gagacgaagg tgaagctctg ggagggcatc tgctacccag
       61 agaacatgcg cgatgactgg caggacgaga tagacgacat tcttcaagtg cctttcgctt
      121 atgcggttca cgacatagac catgaccaga agagcaagca gcgcaagacg catgcgcacg
      181 tcattgttgt ctggggcggc aacacgacac gcaaggcaat catcaacgtc ttgaaccgat
      241 tgagcgcgga cggcaagaag tgctgttcgt cagccgagcc aatcaacaac atcaggcatg
      301 cgtatgacta tctgattcac gacacggctt cctgtcgcaa gaagggcaag gagctgtatc
      361 cggtcgaagc acgcatcgag ggaaacaact tcgacattgg tcagctcgaa cagctctcga
      421 ctaaggacaa gcaggacatg ctcttcgagt tggtcggctt catcatgtgc gagaggttcg
      481 agaccatcaa cgatttcacg gctgcggcgc tgagggagtt tcccgagcag taccgcgaaa
      541 tcatcgtggg gtacaactcg attctcgagc gctattgtcg cgggaactat ctgaacgccg
      601 aacgtaagcg caagtgcgtg gaacagtcag gtgccaaaga gtagtcgggt caatgcagca
      661 atgcgtgact acggggcggc gtggtctgcg ctcgcgcaga tctaagccga ctcgttcacg
      721 gattgcggaa ttgacgcgac ggccaaccga atagaccaga cttgattaag ggtcgatgga
      781 cgctctgctc catcgaccct ttccttgccc gtgtctcgac tggatgagac ctgcagaatc
      841 gggcggctca ttatttttcg ctaactacgt ccgaaaaata atgtgtccga aaaataatgt
      901 gacaaatgac aaatgacaaa tgaccacagg ggggccacta cgacggcccc cctaatagtg
      961 ccgtgtgccg tgtgccgaac cgcaggtaga ttgaggggtg gttcggaggg gcgaagtccc
     1021 tccgttcggc gctcgcgccg atggcccgag ggccgtgagc gaagccgcga acgggcaagg
     1081 gctggggggt ctcgggggga acgcccgagc gggtgcaggg ctggccctgc cacgtcctta
     1141 gcgtagctaa ggtctaacgt gctagacttt agtcgattaa gtctgtacgg ttcggaggtt
     1201 tccatggctc acatcgcgaa atacaaggct ccatcggtcg ggcacatgct tgcccactac
     1261 cgccgcgacc gcagcagcct ggagcgcgac aacatcgacc ccgagcgcat caagaacgac
     1321 ttggtggtcg gccactacac gaacaaggac ggcaagctcg ttgtggggcg cgtggagccg
     1381 cgcgatggcg agccgaactg gggcacggtg gagagccgca tcgagcgcgt gaacgaggcc
     1441 cagaaggccg cagggaagcg tgccacgcgc aaggatgcgg tcgttatggc tgacgttgtt
     1501 gtgacgcttc ccgacaatgt gcgcaagggc gatgaggaca ggttcttccg tctcacgtac
     1561 tggtacctgt cgaagaagtt cggcatcgag aacatgatgg gcgggtacgt ccacaaggac
     1621 gaggtgttga aggatggcac gcccgcccgc gaccacatgc acgtgccgtt cacgcccatc
     1681 ctagatggac ggttcaacta caagcagatg tgcccccgca gcttctaccg gagcatgcac
     1741 aaggagcttg gggattacct agaggggcgc atgggctacc gccctgcaat cgagcttgac
     1801 gaggagacgc gggcgcagcg tgtatacacg gacaagtccg tggatatcga caaggtacgc
     1861 tgtgcggtcg ataaggccgt ggttgaacct gcgaaggacg aagcggcgcg aatcgtcgcg
     1921 gcggctaggg aagaagccgc cgctttactg aatgatgccg aaacgcgtaa ggcagagctt
     1981 gttaccgaga ttgccgacaa ggagggtgac cttgccgagc ttgacagcca gctcgaggac
     2041 gtgaagctcg atatcgaaga tgagcaagac cgattggagt gtcttcggca acgagcggac
     2101 ggagttgcgc gagacattgg agagcttcaa cccatcgcta ccgaggttcg gggctgggaa
     2161 gctgcgggaa aagctgagcg cggcgcaatc ctcgataaca tcgttgttaa gtgcgatgga
     2221 ctcgcgagcc gcatcagagc aggagttgcg ggaatgcgaa tcaaagtgga agagcttaga
     2281 agtcgaatat cgcgcccgct cgaatatctg atgcgcaggg aacagccaag gcaacagagc
     2341 cttaatgacg tgctgcgcga tgcgcagcgg gcggcggatg cgtggaaccg cgaccacgct
     2401 gcaccgcaga ggacgtatcg cgggcgggct agatgatgtg ttatagtagt acctgtaata
     2461 cacgacaagg aggtgcgacg cgaaaaaacc ccgctgaagc ggggtttttt gcgcaccaca
     2521 gcagacagca gaaaacattc tgaacgctag gaagccggta tgattgttac atataagggg
     2581 aagaaaaatt tcttttaggt acttgctttc ctaaaactga tgtgatacaa tgatttaatc
     2641 cagaaaagga gtaaaaaata tgcggcaagg tattcttaaa taaaactata atcaaatagt
     2701 gggaacaaag gattatgata gctccttttg tagggactta gttttttgta cccaatttaa
     2761 gaatactttt gccttatcaa ttttgacata tccccaaaaa cagcaatcac aaacaggtgt
     2821 atgctgtata tgtgtatgtc cgcaacttat aatccccagt ggtaaaagta ttttactgct
     2881 ggggattttt atgccctttg gggctgtaaa gggaggacaa tcacatgaaa ataatcaata
     2941 ttggaattct tgcccatgta gacgctggaa agacgacctt gacggagagc ctgctatatg
     3001 ccagcggagc catttcagaa ccggggagcg tcgaaaaagg gacaacgagg acggacacca
     3061 tgtttttgga gcggcagcgt gggattacca ttcaagcggc agtcacttcc ttccagtggc
     3121 acagatgtaa agttaacatt gtggatacgc ccggccacat ggattttttg gcggaggtgt
     3181 accgctcttt ggctgtttta gatggggcca tcttggtgat ctccgctaaa gatggcgtgc
     3241 aggcccagac ccgtattctg ttccatgccc tgcggaaaat gaacattccc accgttatct
     3301 ttatcaacaa gatcgaccag gctggcgttg atttgcagag cgtggttcag tctgttcggg
     3361 ataagctctc cgccgatatt atcatcaagc agacggtgtc gctgtccccg gaaatagtcc
     3421 tggaggaaaa taccgacata gaagcatggg atgcggtcat cgaaaataac gatgaattat
     3481 tggaaaagta tatcgcagga gaaccaatca gccgggaaaa acttgcgcgg gaggaacagc
     3541 agcgggttca agacgcctcc ctgttcccag tctatcatgg cagcgccaaa aatggccttg
     3601 gcattcaacc gttgatggat gcggtgacag ggctgttcca accgattggg gaacaggggg
     3661 gcgccgccct atgcggcagc gttttcaagg ttgagtacac cgattgcggc cagcggcgtg
     3721 tctatctacg gttatacagc ggaacgctgc gcctgcggga tacggtggcc ctggccggga
     3781 gagaaaagct gaaaatcaca gagatgcgta ttccatccaa aggggaaatt gttcggacag
     3841 acaccgctta tcagggtgaa attgttatcc ttcccagcga cagcgtgagg ttaaacgatg
     3901 tattagggga ccaaacccgg ctccctcgta aaaggtggcg cgaggacccc ctccccatgc
     3961 tgcggacggc gattgcgccg aaaacggcag cgcaaagaga acggctgctg gacgctctta
     4021 cgcaacttgc ggatactgac ccgcttttgc gttgcgaagt ggattccatc acccatgaga
     4081 tcattctttc ttttttgggc cgggtgcagt tggaggttgt ttccgctttg ctgtcggaaa
     4141 aatacaagct tgaaacagtg gtaaaggaac cctccgtcat ttatatggag cggccgctca
     4201 aagcagccag ccacaccatc catatcgagg tgccgcccaa cccgttttgg gcatccatag
     4261 gactgtctgt tacaccactc tcgcttggct ccggtgtaca atacgagagc cgggtttcgc
     4321 tgggatactt gaaccagagt tttcaaaacg ctgtcaggga tggtatccgt tacgggctgg
     4381 agcagggctt gttcggctgg aacgtaacgg actgtaagat ttgctttgaa tacgggcttt
     4441 attacagtcc ggtcagcacg ccggcggact tccgctcatt ggccccgatt gtattggaac
     4501 aggcattgaa ggaatcgggg acgcagctgc tggaacctta tctctccttc atcctctatg
     4561 cgccccagga atacctttcc agggcttatc atgatgcacc gaaatactgt gccaccatcg
     4621 aaacggccca ggtaaaaaag gatgaagttg tctttactgg cgagattccc gcccgctgta
     4681 tacaggcata ccgtactgat ctggcctttt acaccaacgg gcggagcgta tgccttacag
     4741 agctgaaagg atatcaggcc gctgtcggtc agccggtcat ccagccccgc cgtccaaaca
     4801 gccgcctgga caaggtgcgc catatgtttc agaaggtaat gtaaatcgtt aagggatcaa
     4861 ctttgggaga gagttcaaaa ttgatccttt ttttataaca ggaattaaat aataaaaaag
     4921 ccggattaat aatctggctt tttatattct ctctctagta tataaacgca gaaaggccca
     4981 cccgaaggtg agccagtgtg agtcgactag ttcatgcctt cttctgctcg tcgatggtct
     5041 gctcgaccag attgaggatc tcctgctggt tgtggacgtc catcttcgca tagatgttgc
     5101 gaaggtgggt cttcgcggtg gactgcgtga tgacgagcgt gtccgcgata tgctgcgcgg
     5161 tgcggccgcg tgccagcaat acgaggatct cctcctcgcg ccccgtcagc tggtactggt
     5221 cggtgagcgc atggcacgcg ttctgcagct tgttcgcgtg gggaagcgca tcgtgaacgt
     5281 cgggtgcgtc agggccgggc cattgaccgc ggattggcgt gtggtagtcg gtgatgcttc
     5341 ccgaaagcga cgtgatggct ccgcaggcga acaatacgac aagcgcgatg accgagctgg
     5401 acacgaatac gtcgggcgac atatgctcca atagaagaga gctcgccgct ttgcccgcgg
     5461 tgaacgagag gaacgcgacg caccagggca tcaggtaggg tcgtttgctt tcgatgagga
     5521 agggggccag catgataaag aggaacacca gcttctgcgt gatgttcagc aagacgaggt
     5581 agaggaatat ccacgtctcg ttcacgaagg acgacagcca caaggcaaga agcacggtgg
     5641 gtatgaggaa caccttgtac agctcgaggc tccgcctgtt ccagaagtac tggacgagca
     5701 cgaggatggc cgctccgccg catgcggtgc ccacggccgt gcccagctgg atgatgagcg
     5761 ataccgtccc gccgtcttgc aggggaatcc attggtagtg gatatgtccg aacaccacgg
     5821 catagcaagc cagcgacagc atcatcgtca cgacgaatac tcgtcgaccg cgaggagcct
     5881 ggtccgaggg gacgatgagg gatcgatcgg ggcagccgat tcccgccacc atgctccgct
     5941 ccgggctcgc gcaggcatcc tcgctcggga tgtcgtcgcg atccgcacgc ggcgcgcgcg
     6001 aacgggcgta ttccctgaaa tagcagagca gcaccatcga cacgatgggg agcagcgata
     6061 ccgtgccgta agcggcatcc accttcagaa acgcgatgag gctgttcatt gccgcaacga
     6121 cgatcgttcc caggcccagc acggcggcgc aggcgcgcgc cccgtaggga aagagcgctt
     6181 tcatccagag aaacaggatg acgccctctc caagcccgaa tacgatgcgc gcgatggagg
     6241 atacgatggg gttgtcctcc catacgcttt gctgcaagac gacgaacatg gaagccgaca
     6301 tcaacgctcc tgcgccgatg acgatcgcgg cgggaacgtg ggtgggcgcg tccagcttgc
     6361 gatacgcaag agccagcatg atcaggagaa ggaacgatac gagcgacgcg acggcgtggt
     6421 agtcgccgct gatattgacg gttctgatcg tcgagaggcg gtcgccttcg atggcgagca
     6481 gcgcgcatcc gaagccgatc gaagcgatcg cgattcccgg cacctcgcga atcgccagcg
     6541 caagcggcga cgatcctcgt tcgtctgtca tgcggacccc cttttactca catccccatg
     6601 gcgataaggc tcgttcgagc attgtcgtca gtatagcaat tcgccgatac cgcggcggcg
     6661 ggcggatggc gaaataccac tctcggtggt aggtctttca tgagctcgat agtagggttt
     6721 ctgacctggt tgaacagagt tcgcatcagc ggtggggtgc tcaactcgcg ctcgatctgc
     6781 atcatggttc gcacggacgc ttggtccggc ccgctcgttg gagtgcgggt gatctagaag
     6841 cggccgcgaa ttcagaatag taatacagga tccgaatcgt ttcagttgcc gccggattaa
     6901 taatcaaata accgttccta aaaaatgccc tcttgggtta tcaagagggt cattatattt
     6961 cgcaaaaaac cccgcttcgg cggggttttt tcgccttgaa gacgaaaggg cctcgtgata
     7021 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
     7081 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     7141 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     7201 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     7261 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     7321 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     7381 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     7441 cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     7501 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     7561 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     7621 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     7681 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     7741 cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     7801 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     7861 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     7921 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     7981 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     8041 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     8101 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     8161 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     8221 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     8281 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     8341 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
     8401 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     8461 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     8521 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     8581 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     8641 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     8701 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     8761 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     8821 aacgccagca acgcggcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat
     8881 ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc
     8941 tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcatgagc tcctctagta
     9001 gagagcgttc accgacaaac aacagataaa acgaaaggcc cagtctttcg actgagcctt
     9061 tcgttttatt tgaaaaagaa aagccccagc aagcttggcg gcagcagggg cattcgagac
     9121 aaggagatta ta
//
