LOCUS       sequence-375985-        6031 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  nmr-1 fluorescent neural reporter driving nuclear CYOFP expression 
            (refer to NeuroPAL paper for expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6031)
  AUTHORS   Yemini E, Lin A, Nejatbakhsh A, Varol E, Sun R, Mena GE, Samuel ADT,
            Paninski L, Venkatachalam V, Hobert O
  TITLE     NeuroPAL: A Multicolor Atlas for Whole-Brain Neuronal Identification
            in C. elegans.
  JOURNAL   Cell. 2021 Jan 7;184(1):272-288.e11. doi: 
            10.1016/j.cell.2020.12.012. Epub 2020 Dec 29.
  PUBMED    33378642
REFERENCE   2  (bases 1 to 6031)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.012"; journalName: "Cell"; date: "2021-01-7-
            7"; volume: "184"; issue: "1"; pages: "272-288.e11"
COMMENT     Sequence Label: pEY32
FEATURES             Location/Qualifiers
     source          1..6031
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6..22
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1390..1410
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1432..1452
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(3622..3644)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3744..3763
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(3841..3859)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3927..4031
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4032..4100,4101..4892)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4250..4269)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5063..5651
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5552..5571
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5805..5822
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5939..5960
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5975..5980,5981..5998,5999..6005)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6013..6029
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(6018..6031,1..9)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcctgcagc tgctgctgta
      181 ggctttgcaa caaccatttc tgaaagtata agatttgact gaaaattata aaattatgaa
      241 cttgagaaag tctagaaatc aagaaaaaat atctagaaat gaacattcca tgggttgtga
      301 agatgctttt atctcacttt agaatgtctg actccgcccc tctgtgtacg aattgaagtg
      361 ctcttttgtg atgatcatag ctcctccttg cggcgacgcc cgcacacagc acgagatatt
      421 acacggaata cttatgtgat tcataggaaa caaggaaatc gaaaaaataa taggattttg
      481 aggaggtatt tttagatgaa tgcttatgat gttatgatga acccaagaaa ttctgaatag
      541 acatatgtca gaacggggta ggagctccag tgtcgctttt gaaataaaaa atcaagtggt
      601 atacttctcg tatataccct tctgaactaa taaaataatt tatcttttga aaaagtttga
      661 aaaagaaaaa tgcccaaaac ttgaatttta atgttttgta ttttgagcat atcgttcaaa
      721 cgtcgtgatc tgaaagtaga aagatctaaa tattcgtgac acagtttggt ttgaaggtct
      781 tagtggtaat tttttattac agaagcaaca aaaagttgac atattttcag aatttccaaa
      841 aaaacgttta aaatttcaat ttcttcgata gagttctaaa aattttttgg aacggatttt
      901 gtaatatccg ctgataaccg ttcaagaaca atttgaattt tgaaataatt taatcaacta
      961 gtatccagtg ctctttctca ctgctccacc catttttcaa cccatttcct ttactcaatt
     1021 tcgcccgttt tccttttgct caaatgtgcc caccaaatca tctttcttcg tcttcttttc
     1081 tcttattcgg aaggagaaga ggtgaaagta gaatacgttt ttttgttgaa aagagaagag
     1141 cactgccact tttctcttat ttccatcctt ctactacctc gttttacttt ccatttaaaa
     1201 acccgtttaa agttccaaaa tttcaattat cttgcttctg tttggaacac gacaaacttt
     1261 agttttgtta cagatcccgg gattggccaa aggacccaaa ggtatgtttc gaatgatact
     1321 aacataacat agaacatttt caggaggacc cttgcttgga gggtaccgag ctcagaaaaa
     1381 atgaccgctc caaagaagaa gcgcaaggtt ccagtcgaga agatgactgc cccaaaaaag
     1441 aaacgtaagg tcccgcgggt aagtttaaac agttcggtac taactaacca tacatattta
     1501 aattttcagc gcgctgtgtc aaaaggagaa gagcttatta aggagaatat gcgttccaag
     1561 ctctacctcg agggatccgt caacggacac caattcaagt gcacccacga gggagaggga
     1621 aagccatacg agggaaagca aaccaaccgt atcaaggtcg tcgagggagg accactccca
     1681 ttcgccttcg acatcctcgc cacccacttc atgtacggat ccaaggtaag tttaaacata
     1741 tatatactaa ctaaccctga ttatttaaat tttcaggtct tcatcaagta cccagccgac
     1801 ctcccagact acttcaagca atccttccca gagggattca cctgggagcg tgtcatggtc
     1861 ttcgaggacg gaggagtcct caccgccacc caagacacct ccctccaaga cggagagctc
     1921 atctacaacg tcaaggtccg tggagtcaac ttcccagcca acggaccagt catgcaaaag
     1981 aagaccctcg gatgggagcc atccaccgag accatgtacc cagccgacgg aggactcgag
     2041 ggacgttgcg acaaggtaag tttaaacatg attttactaa ctaactaatc tgatttaaat
     2101 tttcaggccc tcaagctcgt cggaggagga cacctccacg tcaacttcaa gaccacctac
     2161 aagtccaaga agccagtcaa gatgccagga gtccactacg tcgaccgtcg tctcgagcgt
     2221 atcaaggagg ccgacaacga gacctacgtc gagcaatacg agcacgccgt cgcccgttac
     2281 tccaacctcg gaggaggaat ggacgagctc tacaagccta ggataccggt gggtgaagac
     2341 ccaccaaagc catctgccaa gggagccaag aaggccgcca agaccgttac gaagccaaag
     2401 gacggaaaga agagacgtca tgcccgtaag gaatcatact ccgtctacat ctaccgtgtc
     2461 ctcaagcaag ttcatccaga cactggagtt tcctccaaag ccatgtctat catgaactct
     2521 tttgtcaacg atgtcttcga gcgtattgct gctgaagcat cccgtcttgc tcactacaac
     2581 aagcgttcca caatctcatc ccgcgaaatt cagaccgctg tccgtctgat ccttccagga
     2641 gagcttgcca agcacgccgt gtctgaggga accaaggccg ttaccaagta cacttccagc
     2701 aagtaggaat tccaactgag cgccggtcgc taccattacc aacttgtctg gtgtcaaaaa
     2761 taataggggc cgctgtcatc agagtaagtt taaactgagt tctactaact aacgagtaat
     2821 atttaaattt tcagcatctc gcgcccgtgc ctctgacttc taagtccaat tactcttcaa
     2881 catccctaca tgctctttct ccctgtgctc ccacccccta tttttgttat tatcaaaaaa
     2941 cttctcttaa tttctttgtt ttttagcttc ttttaagtca cctctaacaa tgaaattgtg
     3001 tagattcaaa aatagaatta attcgtaata aaaagtcgaa aaaaattgtg ctccctcccc
     3061 ccattaataa taattctatc ccaaaatcta cacaatgttc tgtgtacact tcttatgttt
     3121 tttacttctg ataaattttt ttgaaacatc atagaaaaaa ccgcacacaa aataccttat
     3181 catatgttac gtttcagttt atgaccgcaa tttttatttc ttcgcacgtc tgggcctctc
     3241 atgacgtcaa atcatgctca tcgtgaaaaa gttttggagt atttttggaa tttttcaatc
     3301 aagtgaaagt ttatgaaatt aattttcctg cttttgcttt ttggggtttc ccctattgtt
     3361 tgtcaagatt tcgaggacgg cgtttttctt gctaaaatca caagtattga tgagcacgat
     3421 gcaagaaaga tcggaagaag gtttgggttt gaggctcagt ggaaggtgag tagaagttga
     3481 taatttgaaa gtggagtagt gtctatgggg tttttgcctt aaatgacaga atacattccc
     3541 aatataccaa acataactgt ttcctactag tcggccgtac gggccctttc gtctcgcgcg
     3601 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg
     3661 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg
     3721 gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat
     3781 gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc ggccttaagg
     3841 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
     3901 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     3961 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     4021 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     4081 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     4141 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     4201 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     4261 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     4321 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     4381 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     4441 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     4501 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     4561 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     4621 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     4681 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     4741 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     4801 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     4861 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     4921 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     4981 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     5041 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     5101 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     5161 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     5221 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     5281 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     5341 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     5401 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     5461 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     5521 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     5581 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     5641 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     5701 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     5761 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     5821 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     5881 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     5941 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     6001 tgttgtgtgg aattgtgagc ggataacaat t
//
