LOCUS       sequence-375987-       11634 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  E. coli - Eggerthella lenta shuttle plasmid (KanR), Phcdh 
            hydrocaffeic acid dehydroxylase promoter-lacZ with transcriptional 
            regulator HcdR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11634)
  AUTHORS   Dong X, Guthrie BGH, Alexander M, Noecker C, Ramirez L, Glasser NR, 
            Turnbaugh PJ, Balskus EP
  TITLE     Genetic manipulation of the human gut bacterium Eggerthella lenta 
            reveals a widespread family of transcriptional regulators.
  JOURNAL   Nat Commun. 2022 Dec 9;13(1):7624. doi: 10.1038/s41467-022-33576-3.
  PUBMED    36494336
REFERENCE   2  (bases 1 to 11634)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-12-9"; volume: "13(1):7624. doi"; pages: " 
            10.1038/s41467-022-33576-3"
COMMENT     Sequence Label: pXD70LacZ9.1-HcdR-Phcdh-LacZ
FEATURES             Location/Qualifiers
     source          1..11634
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(157..261)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2364..2382
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    2539..2558
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     CDS             2830..5904
                     /codon_start=1
                     /gene="lacZ"
                     /product="beta-galactosidase"
                     /label=lacZ
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART
                     DRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNV
                     TYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYG
                     QDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQ
                     ISDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIID
                     ERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIEN
                     GLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLW
                     YTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWS
                     LGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVP
                     KWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSL
                     IKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQ
                     TIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQ
                     LWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELG
                     NKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWK
                     AAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDV
                     EVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPY
                     VFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDG
                     FHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
     primer_bind     complement(2850..2867)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2850..2866)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2859..2881)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      5922..5949
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(6072..6866)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     terminator      9919..9990
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      complement(10171..10759)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10251..10270)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10930..11634,1..87,88..156))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11553..11572
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
       61 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
      121 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
      181 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
      241 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac
      301 cattattaaa aaaaaacccc gccgaagcgg ggtttttttt tatctattcg gcgggatccg
      361 caagctcgac gaggcgcatg agctcctgct ggctgtggac gttcgtcttc ttgtagacgt
      421 ggcggatgtg ggttttcgcg gtgccttccg agatgtagag cttttcctgg atgtacgcgg
      481 cgttgtgccc cttcgccaga aagaacatga tctccgtctc gcgccgggaa agcaggtagg
      541 tgttggcaac cgtctcgcac ttcgcgcgga agcggccgcc tccctttcgc tcttcgccct
      601 cgtctgctcg gcgtgcgctg ctgagttcca gcagcgaagg ggcgcgccgc gcggtcggtt
      661 cgggttcggg cgcgatctcg gcgtcaggtg caggcgcgtt ggtcgctgcc gtcgcgttgt
      721 ttccagattg cgcggccgcg gcggctccct cgccggccga tcccgcttgc cctagcggcc
      781 gcacgcggtc gctcaattcc aacgacacgg ctttcaccag cggacacggt gcgacgatgg
      841 cctcgatctc gcgctcgcgc ggcagcagcg cgtatgccac caccataatc agcagcacga
      901 tgacgatgac gccctgttcg ccgaacggaa gcagatgcac ccagttcgcc gccacgatgg
      961 gaaacagcga acccgccagc cccgccagcg ccaacatgcc gcgtcccagg ccgaacacgt
     1021 agatgggcga caagcggaag cgttgcgcca gctcgccgaa gaatatccac atcagcgcct
     1081 cgaagcacag gtagcccatc accaagaaca tggtggcgaa cgtgttgtcg ctcatttcgg
     1141 aaagcggcag gaagaacagc gtgacggcga tgaaggggac gagcgggcgg aagtaccggc
     1201 tccatttgtc gccgccgcca agggccacga tggtcacgag gatgactacg gaggcgaatc
     1261 cggcggccag cagcatggcg atctggtcgc ctacgttcga ggcgggcacg atgtactgga
     1321 tggacgtttg ccgcagcgtg cccaacgcca cgccgaacac gaacacggga atgccgaagc
     1381 gcaggaagaa cttggcatgg ttcaccggaa gcggtttgaa gatgggaacc tcgttgcgct
     1441 cggaatacgg cgccggcgtt ttgttccaga ggatggcgag ctccagcagc gggatgatgc
     1501 ccgcgaggat gccggcgatg gggaacggcc cgtaatgcag gccgatggcg tacaccccga
     1561 tggcgatgga gatggcgatg gacgagttca gcacgatgga agcgctgtcg cagcgcgcga
     1621 acgcgacgcc ccagaacaga atcaggatgg cggagccgat gcccgtcatg acgcccgaca
     1681 ccgcttccag cgccgggccg ctgatcacgg gcgtcgcaag cagcagcaac gttccggccg
     1741 acatgagcgc cgcgcccgcc gcaagcgtct tcttcgagat gtacgtcctc aacagccgct
     1801 ggtcggtgat gccggcgaac aacaggcaca gcccgaacac gacgatggag acgaggaacg
     1861 gcagcgacgc gtggctgccg tacatgccgg tgacgtaggt ttgcgtgccg aagatggtgg
     1921 gcgttccgaa catgacggcg tacacccaag cctggtgcag tccgtagccc accgagagca
     1981 agctcaggtc gccgaacgcg gtgtcgtcgc cgaagtcggc gttgacggtg atgcgaggaa
     2041 cgcgaatccc catgaagccc cctccttgat tccctgggca tcatcatacc gtgcagaggt
     2101 tgttttttct agcgaggtga gcgagtcgat cagggttttg cgctcgatgc gcaaacgttt
     2161 gatctggggt aatagaaaaa taatcctctg aggatgatgt tcggaagggc gcccgtctgc
     2221 tttcgagggc gtgctgaggc gtcgcggtgc tgcatcgggc cggcttgtga agaataacgg
     2281 ggcgcttgcc gagccggaac gggaggtgtg cgggggcccg gctggtcgtc cgaacccccg
     2341 cgcggcggac attaacctat aaaaataggc gtatcacgag gccctttcgt cttcaaggcg
     2401 aaaaaacccc gccgaagcgg ggttttttgc gaaatataat gaccctcttg ataacccaag
     2461 agggcatttt ttaggaacgg ttatttgatt attaatccgg cggcaactga aacgattcgg
     2521 atcctgtatt actattctga attcgcggcc gcttctagat ggcagtctcc ttcgttcaca
     2581 ggtttcttcc cgttgccgca cgcccggatg acgggcgcga acggcaacct cttcgctgcc
     2641 atcaagtata ggttgggcgc gaaaagcaaa ataatccttt ggggatgata tcggcgcgtt
     2701 tcatccatcc accccttatg atttgggctt ttgctccgtt cctagaatgg ggaaaccggt
     2761 tccggcgtat ggtcgcgccg ggttccggca gagacgaacg aagcgagaaa aggaagagag
     2821 gaggggcaca tgaccatgat tacggattca ctggccgtcg ttttacaacg tcgtgactgg
     2881 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
     2941 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     3001 gaatggcgct ttgcctggtt tccggcacca gaagcggtgc cggaaagctg gctggagtgc
     3061 gatcttcctg aggccgatac tgtcgtcgtc ccctcaaact ggcagatgca cggttacgat
     3121 gcgcccatct acaccaacgt gacctatccc attacggtca atccgccgtt tgttcccacg
     3181 gagaatccga cgggttgtta ctcgctcaca tttaatgttg atgaaagctg gctacaggaa
     3241 ggccagacgc gaattatttt tgatggcgtt aactcggcgt ttcatctgtg gtgcaacggg
     3301 cgctgggtcg gttacggcca ggacagtcgt ttgccgtctg aatttgacct gagcgcattt
     3361 ttacgcgccg gagaaaaccg cctcgcggtg atggtgctgc gctggagtga cggcagttat
     3421 ctggaagatc aggatatgtg gcggatgagc ggcattttcc gtgacgtctc gttgctgcat
     3481 aaaccgacta cacaaatcag cgatttccat gttgccactc gctttaatga tgatttcagc
     3541 cgcgctgtac tggaggctga agttcagatg tgcggcgagt tgcgtgacta cctacgggta
     3601 acagtttctt tatggcaggg tgaaacgcag gtcgccagcg gcaccgcgcc tttcggcggt
     3661 gaaattatcg atgagcgtgg tggttatgcc gatcgcgtca cactacgtct gaacgtcgaa
     3721 aacccgaaac tgtggagcgc cgaaatcccg aatctctatc gtgcggtggt tgaactgcac
     3781 accgccgacg gcacgctgat tgaagcagaa gcctgcgatg tcggtttccg cgaggtgcgg
     3841 attgaaaatg gtctgctgct gctgaacggc aagccgttgc tgattcgagg cgttaaccgt
     3901 cacgagcatc atcctctgca tggtcaggtc atggatgagc agacgatggt gcaggatatc
     3961 ctgctgatga agcagaacaa ctttaacgcc gtgcgctgtt cgcattatcc gaaccatccg
     4021 ctgtggtaca cgctgtgcga ccgctacggc ctgtatgtgg tggatgaagc caatattgaa
     4081 acccacggca tggtgccaat gaatcgtctg accgatgatc cgcgctggct accggcgatg
     4141 agcgaacgcg taacgcgaat ggtgcagcgc gatcgtaatc acccgagtgt gatcatctgg
     4201 tcgctgggga atgaatcagg ccacggcgct aatcacgacg cgctgtatcg ctggatcaaa
     4261 tctgtcgatc cttcccgccc ggtgcagtat gaaggcggcg gagccgacac cacggccacc
     4321 gatattattt gcccgatgta cgcgcgcgtg gatgaagacc agcccttccc ggctgtgccg
     4381 aaatggtcca tcaaaaaatg gctttcgcta cctggagaga cgcgcccgct gatcctttgc
     4441 gaatacgccc acgcgatggg taacagtctt ggcggtttcg ctaaatactg gcaggcgttt
     4501 cgtcagtatc cccgtttaca gggcggcttc gtctgggact gggtggatca gtcgctgatt
     4561 aaatatgatg aaaacggcaa cccgtggtcg gcttacggcg gtgattttgg cgatacgccg
     4621 aacgatcgcc agttctgtat gaacggtctg gtctttgccg accgcacgcc gcatccagcg
     4681 ctgacggaag caaaacacca gcagcagttt ttccagttcc gtttatccgg gcaaaccatc
     4741 gaagtgacca gcgaatacct gttccgtcat agcgataacg agctcctgca ctggatggtg
     4801 gcgctggatg gtaagccgct ggcaagcggt gaagtgcctc tggatgtcgc tccacaaggt
     4861 aaacagttga ttgaactgcc tgaactaccg cagccggaga gcgccgggca actctggctc
     4921 acagtacgcg tagtgcaacc gaacgcgacc gcatggtcag aagccgggca catcagcgcc
     4981 tggcagcagt ggcgtctggc ggaaaacctc agtgtgacgc tccccgccgc gtcccacgcc
     5041 atcccgcatc tgaccaccag cgaaatggat ttttgcatcg agctgggtaa taagcgttgg
     5101 caatttaacc gccagtcagg ctttctttca cagatgtgga ttggcgataa aaaacaactg
     5161 ctgacgccgc tgcgcgatca gttcacccgt gcaccgctgg ataacgacat tggcgtaagt
     5221 gaagcgaccc gcattgaccc taacgcctgg gtcgaacgct ggaaggcggc gggccattac
     5281 caggccgaag cagcgttgtt gcagtgcacg gcagatacac ttgctgatgc ggtgctgatt
     5341 acgaccgctc acgcgtggca gcatcagggg aaaaccttat ttatcagccg gaaaacctac
     5401 cggattgatg gtagtggtca aatggcgatt accgttgatg ttgaagtggc gagcgataca
     5461 ccgcatccgg cgcggattgg cctgaactgc cagctggcgc aggtagcaga gcgggtaaac
     5521 tggctcggat tagggccgca agaaaactat cccgaccgcc ttactgccgc ctgttttgac
     5581 cgctgggatc tgccattgtc agacatgtat accccgtacg tcttcccgag cgaaaacggt
     5641 ctgcgctgcg ggacgcgcga attgaattat ggcccacacc agtggcgcgg cgacttccag
     5701 ttcaacatca gccgctacag tcaacagcaa ctgatggaaa ccagccatcg ccatctgctg
     5761 cacgcggaag aaggcacatg gctgaatatc gacggtttcc atatggggat tggtggcgac
     5821 gactcctgga gcccgtcagt atcggcggaa ttccagctga gcgccggtcg ctaccattac
     5881 cagttggtct ggtgtcaaaa ataaactagt cgactcacac tggctcacct tcgggtgggc
     5941 ctttctgcgt ttatatacta gagagagaat ataaaaagcc agattattaa tccggctttt
     6001 ttattattta attcctgtta taaaaaaagg atcaattttg aactctctcc caaagttgat
     6061 cccttaacga tctaaaacaa ttcatccagt aaaatataat attttatttt ctcccaatca
     6121 ggcttgatcc ccagtaagtc aaaaaatagc tcgacatact gttcttcccc gatatcctcc
     6181 ctgatcgacc ggacgcagaa ggcaatgtca taccacttgt ccgccctgcc gcttctccca
     6241 agatcaataa agccacttac tttgccatct ttcacaaaga tgttgctgtc tcccaggtcg
     6301 ccgtgggaaa agacaagttc ctcttcgggc ttttccgtct ttaaaaaatc atacagctcg
     6361 cgcggatctt taaatggagt gtcttcttcc cagttttcgc aatccacatc ggccagatcg
     6421 ttattcagta agtaatccaa ttcggctaag cggctgtcta agctattcgt atagggacaa
     6481 tccgatatgt cgatggagtg aaagagcctg atgcactccg catacagctc gataatcttt
     6541 tcagggcttt gttcatcttc atactcttcc gagcaaagga cgccatcggc ctcactcatg
     6601 agcagattgc tccagccatc atgccgttca aagtgcagga cctttggaac aggcagcttt
     6661 ccttccagcc atagcatcat gtccttttcc cgttccacat cataggtggt ccctttatac
     6721 cggctgtccg tcatttttaa atataggttt tcattttctc ccaccagctt atatacctta
     6781 gcaggagaca ttccttccgt atcttttacg cagcggtatt tttcgatcag ttttttcaat
     6841 tccggtgata ttctcatttt agccatttat tatttccttc ctcttttcta cagtatttaa
     6901 agatacccca agaagctaat tataacaaga cgaactccaa ttcactgttc cttgcattct
     6961 aaaaccttaa ataccagaaa acagcttttt caaagttgtt ttcaaagttg gcgtataaca
     7021 tagtatcgac ggagccgatt ttgaaaccac aattatgata gaatttacaa gctataaggt
     7081 tattgtcctg ggtttcaagc attagtccat gcaagttttt atgctttgcc cattctatag
     7141 atatattgat aagaagactg gaatgcccgc tccgccggga ttattcgaaa gcgcatcgtc
     7201 caagcgagac cgtctgcatt cggaaatctc ggccggttcg cgccgaggaa gcccatctgg
     7261 ctgcatccca aatcgacaca caacgcacgg aggccccggc gatcgccggg gcctccgaat
     7321 catgcgcatc gtggaagcga agctccgcaa aaaaccccgc ttcagcgggg ttttttcgcg
     7381 tcgcacctcc ttgtcgtgta ttacaggtac tactataaca catcatctag cccgcccgcg
     7441 atacgtcctc tgcggtgcag cgtggtcgcg gttccacgca tccgccgccc gctgcgcatc
     7501 gcgcagcacg tcattaaggc tctgttgcct tggctgttcc ctgcgcatca gatattcgag
     7561 cgggcgcgat attcgacttc taagctcttc cactttgatt cgcattcccg caactcctgc
     7621 tctgatgcgg ctcgcgagtc catcgcactt aacaacgatg ttatcgagga ttgcgccgcg
     7681 ctcagctttt cccgcagctt cccagccccg aacctcggta gcgatgggtt gaagctctcc
     7741 aatgtctcgc gcaactccgt ccgctcgttg ccgaagacac tccaatcggt cttgctcatc
     7801 ttcgatatcg agcttcacgt cctcgagctg gctgtcaagc tcggcaaggt caccctcctt
     7861 gtcggcaatc tcggtaacaa gctctgcctt acgcgtttcg gcatcattca gtaaagcggc
     7921 ggcttcttcc ctagccgccg cgacgattcg cgccgcttcg tccttcgcag gttcaaccac
     7981 ggccttatcg accgcacagc gtaccttgtc gatatccacg gacttgtccg tgtatacacg
     8041 ctgcgcccgc gtctcctcgt caagctcgat tgcagggcgg tagcccatgc gcccctctag
     8101 gtaatcccca agctccttgt gcatgctccg gtagaagctg cgggggcaca tctgcttgta
     8161 gttgaaccgt ccatctagga tgggcgtgaa cggcacgtgc atgtggtcgc gggcgggcgt
     8221 gccatccttc aacacctcgt ccttgtggac gtacccgccc atcatgttct cgatgccgaa
     8281 cttcttcgac aggtaccagt acgtgagacg gaagaacctg tcctcatcgc ccttgcgcac
     8341 attgtcggga agcgtcacaa caacgtcagc cataacgacc gcatccttgc gcgtggcacg
     8401 cttccctgcg gccttctggg cctcgttcac gcgctcgatg cggctctcca ccgtgcccca
     8461 gttcggctcg ccatcgcgcg gctccacgcg ccccacaacg agcttgccgt ccttgttcgt
     8521 gtagtggccg accaccaagt cgttcttgat gcgctcgggg tcgatgttgt cgcgctccag
     8581 gctgctgcgg tcgcggcggt agtgggcaag catgtgcccg accgatggag ccttgtattt
     8641 cgcgatgtga gccatggaaa cctccgaacc gtacagactt aatcgactaa agtctagcac
     8701 gttagacctt agctacgcta aggacgtggc agggccagcc ctgcacccgc tcgggcgttc
     8761 cccccgagac cccccagccc ttgcccgttc gcggcttcgc tcacggccct cgggccatcg
     8821 gcgcgagcgc cgaacggagg gacttcgccc ctccgaacca cccctcaatc tacctgcggt
     8881 tcggcacacg gcacacggca ctattagggg ggccgtcgta gtggcccccc tgtggtcatt
     8941 tgtcatttgt catttgtcac attatttttc ggacacatta tttttcggac gtagttagcg
     9001 aaaaataatg agccgcccga ttctgcaggt ctcatccagt cgagacacgg gcaaggaaag
     9061 ggtcgatgga gcagagcgtc catcgaccct taatcaagtc tggtctattc ggttggccgt
     9121 cgcgtcaatt ccgcaatccg tgaacgagtc ggcttagatc tgcgcgagcg cagaccacgc
     9181 cgccccgtag tcacgcattg ctgcattgac ccgactactc tttggcacct gactgttcca
     9241 cgcacttgcg cttacgttcg gcgttcagat agttcccgcg acaatagcgc tcgagaatcg
     9301 agttgtaccc cacgatgatt tcgcggtact gctcgggaaa ctccctcagc gccgcagccg
     9361 tgaaatcgtt gatggtctcg aacctctcgc acatgatgaa gccgaccaac tcgaagagca
     9421 tgtcctgctt gtccttagtc gagagctgtt cgagctgacc aatgtcgaag ttgtttccct
     9481 cgatgcgtgc ttcgaccgga tacagctcct tgcccttctt gcgacaggaa gccgtgtcgt
     9541 gaatcagata gtcatacgca tgcctgatgt tgttgattgg ctcggctgac gaacagcact
     9601 tcttgccgtc cgcgctcaat cggttcaaga cgttgatgat tgccttgcgt gtcgtgttgc
     9661 cgccccagac aacaatgacg tgcgcatgcg tcttgcgctg cttgctcttc tggtcatggt
     9721 ctatgtcgtg aaccgcataa gcgaaaggca cttgaagaat gtcgtctatc tcgtcctgcc
     9781 agtcatcgcg catgttctct gggtagcaga tgccctccca gagcttcacc ttcgtctcgc
     9841 cagcgtttgc cgccatgcta taatctcctt gtctcgaatg cccctgctgc cgccaagctt
     9901 gctggggctt ttctttttca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
     9961 tttatctgtt gtttgtcggt gaacgctctc tactagagga gctcatgccg gcggcacctc
    10021 gctaacggat tcaccactcc aagaattgga gccaatcaat tcttgcggag aactgtgaat
    10081 gcgcaaacca acccttggca gaacatatcc atcgcgtccg ccatctccag cagccgcacg
    10141 cggcgcatct cgggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
    10201 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
    10261 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
    10321 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
    10381 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
    10441 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
    10501 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
    10561 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
    10621 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
    10681 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
    10741 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
    10801 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
    10861 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
    10921 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
    10981 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
    11041 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
    11101 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
    11161 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
    11221 tttgcgcaac gttgttgcca ttgctgcagg catcgtggtg tcacgctcgt cgtttggtat
    11281 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
    11341 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
    11401 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
    11461 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
    11521 accgagttgc tcttgcccgg cgtcaacacg ggataatacc gcgccacata gcagaacttt
    11581 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tctt
//
