LOCUS       sequence-375994-        6943 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  bas-1 fluorescent neural reporter driving nuclear CYOFP expression 
            (refer to NeuroPAL paper for expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6943)
  AUTHORS   Yemini E, Lin A, Nejatbakhsh A, Varol E, Sun R, Mena GE, Samuel ADT,
            Paninski L, Venkatachalam V, Hobert O
  TITLE     NeuroPAL: A Multicolor Atlas for Whole-Brain Neuronal Identification
            in C. elegans.
  JOURNAL   Cell. 2021 Jan 7;184(1):272-288.e11. doi: 
            10.1016/j.cell.2020.12.012. Epub 2020 Dec 29.
  PUBMED    33378642
REFERENCE   2  (bases 1 to 6943)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.012"; journalName: "Cell"; date: "2021-01-7-
            7"; volume: "184"; issue: "1"; pages: "272-288.e11"
COMMENT     Sequence Label: pEY65
FEATURES             Location/Qualifiers
     source          1..6943
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6..22
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2302..2322
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2344..2364
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(4534..4556)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4656..4675
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4753..4771)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4839..4943
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4944..5012,5013..5804)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5162..5181)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5975..6563
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6464..6483
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6717..6734
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6851..6872
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6887..6892,6893..6910,6911..6917)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6925..6941
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(6930..6943,1..9)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcctgcagt tcacagggtc
      181 tggtttcaaa ataaactgca aaatcaaata aatcgcccca ccggcgatga agagccaaaa
      241 cattactgaa attgtaaaaa atcaagttta ataaaaaact ggtagggaaa aggaaaaaat
      301 ggtaaaacca taaaatatgt gaaataaatg attgccaacg catattataa ccagaaaaaa
      361 tgtttgttat cactattgat aagtgtttat cagagtgtgc tctctgctcc aagagggcaa
      421 gctgtttttg tgtttcaaat ggtcgaaata caattggaaa gctatatttg gagttccgaa
      481 ttgtgaattg tcatgatctg gaattaaacg acaatttttt ggcatttccg atatctattg
      541 aaaaaattgg gtggtttgag aaacatttgg caactcgaat aatttttgaa ttatgctata
      601 actttttgga gaagatcaac tttttttaaa ataccaactt cgaagtcacc tgcaactttt
      661 tgaagaaaaa ttgttttgga aatggcaaca tcttagactt ttttgcaaac attcggcaac
      721 ttttgaacat atgtatttag caacttttgg agcatattgg catttgccag ttttaatcga
      781 tcgcaaacgt tttggagaat atcgacaact ttaggaagtc atcggcagtt ccgaaaattt
      841 tcggcaccta atcagcttca tagattcgtt tcgatcacta tcctatcccc ggctttctgc
      901 cagaattcca gtaacacatt gatattcttc cccaacacca cattattcat gtatttcctc
      961 caaaccactg aaccatctca ttctcaaacc agtttctatc cgtttgtttg cattcaatta
     1021 aatttttttt ttcagcgtat tcttctttgc actgggatga atggaaaagt aggtggagag
     1081 gcaatgacaa aaattattta ttattcctgt cttatttctt tcaaagtaat ttatttgcac
     1141 tctcgactga agtttttcca actttacgtg tcagctttgt acctgcctgc ctacctaaaa
     1201 aaagcggcat gtacattggc agcaggctcg tgtgtcagac atgggtaaaa ttattccata
     1261 aaagatattt caattcatat ttgttggcta gtttttagcc tgcctgcttt tttgagccta
     1321 acacgtggat tgtggaagga ccaattagaa agtgtacatg cgcctttgaa ggttgagtag
     1381 tgtcaattgg gaatatgtta aaaaaaaatt tttattttat attctaatag aaagtagatt
     1441 tcaaatactt gttccttaac gtcgtaggtg tgaaaacatc cgacatcgtt tgaaaattct
     1501 cacgaaatca atcaaaactt ttcttaaatt tcacaattta ccaaaacatt gactgaaaac
     1561 ctaaaatttt tggagtgttt catcgtgcat tttggacatt ttgggacatt ataagtgaat
     1621 ttagacatag ttctcagaca gaaaattgtg tgaaaatcaa taattagacc ctcccgttgc
     1681 cgacgctcat catcactgac caaattgata agatgataag gatgagtgaa atagacgact
     1741 tttggtcaaa aaatcattgt catctcggag attgaatttt gttttattta tcattctgca
     1801 tattatgttg agtttgagtg tctgaagttt ggcaccttcg ccaatatttc cgttttcaga
     1861 acttttgaca atttctgcag ccaattcttt ttctccagaa gcttcatcac atcatcattt
     1921 tcttattctt ccattcacct aaatccatca ttctcctccc ccgtcctttt ctctttgcga
     1981 cgcccatttc cggataagag gcctcgcgca tagatggata ggtatcggaa atgtgctcgg
     2041 ttcccatagc gaaattatat ttcttatttt cttattttcc cctcatttct cttattttcc
     2101 tacctccttc gatctctgtc aatcgtggga aatctttttg ctccagcttt acaatttttg
     2161 tcgaactttc agtagtagtt cggtataccc gggattggcc aaaggaccca aaggtatgtt
     2221 tcgaatgata ctaacataac atagaacatt ttcaggagga cccttgcttg gagggtaccg
     2281 agctcagaaa aaatgaccgc tccaaagaag aagcgcaagg ttccagtcga gaagatgact
     2341 gccccaaaaa agaaacgtaa ggtcccgcgg gtaagtttaa acagttcggt actaactaac
     2401 catacatatt taaattttca gcgcgctgtg tcaaaaggag aagagcttat taaggagaat
     2461 atgcgttcca agctctacct cgagggatcc gtcaacggac accaattcaa gtgcacccac
     2521 gagggagagg gaaagccata cgagggaaag caaaccaacc gtatcaaggt cgtcgaggga
     2581 ggaccactcc cattcgcctt cgacatcctc gccacccact tcatgtacgg atccaaggta
     2641 agtttaaaca tatatatact aactaaccct gattatttaa attttcaggt cttcatcaag
     2701 tacccagccg acctcccaga ctacttcaag caatccttcc cagagggatt cacctgggag
     2761 cgtgtcatgg tcttcgagga cggaggagtc ctcaccgcca cccaagacac ctccctccaa
     2821 gacggagagc tcatctacaa cgtcaaggtc cgtggagtca acttcccagc caacggacca
     2881 gtcatgcaaa agaagaccct cggatgggag ccatccaccg agaccatgta cccagccgac
     2941 ggaggactcg agggacgttg cgacaaggta agtttaaaca tgattttact aactaactaa
     3001 tctgatttaa attttcaggc cctcaagctc gtcggaggag gacacctcca cgtcaacttc
     3061 aagaccacct acaagtccaa gaagccagtc aagatgccag gagtccacta cgtcgaccgt
     3121 cgtctcgagc gtatcaagga ggccgacaac gagacctacg tcgagcaata cgagcacgcc
     3181 gtcgcccgtt actccaacct cggaggagga atggacgagc tctacaagcc taggataccg
     3241 gtgggtgaag acccaccaaa gccatctgcc aagggagcca agaaggccgc caagaccgtt
     3301 acgaagccaa aggacggaaa gaagagacgt catgcccgta aggaatcata ctccgtctac
     3361 atctaccgtg tcctcaagca agttcatcca gacactggag tttcctccaa agccatgtct
     3421 atcatgaact cttttgtcaa cgatgtcttc gagcgtattg ctgctgaagc atcccgtctt
     3481 gctcactaca acaagcgttc cacaatctca tcccgcgaaa ttcagaccgc tgtccgtctg
     3541 atccttccag gagagcttgc caagcacgcc gtgtctgagg gaaccaaggc cgttaccaag
     3601 tacacttcca gcaagtagga attccaactg agcgccggtc gctaccatta ccaacttgtc
     3661 tggtgtcaaa aataataggg gccgctgtca tcagagtaag tttaaactga gttctactaa
     3721 ctaacgagta atatttaaat tttcagcatc tcgcgcccgt gcctctgact tctaagtcca
     3781 attactcttc aacatcccta catgctcttt ctccctgtgc tcccaccccc tatttttgtt
     3841 attatcaaaa aacttctctt aatttctttg ttttttagct tcttttaagt cacctctaac
     3901 aatgaaattg tgtagattca aaaatagaat taattcgtaa taaaaagtcg aaaaaaattg
     3961 tgctccctcc ccccattaat aataattcta tcccaaaatc tacacaatgt tctgtgtaca
     4021 cttcttatgt tttttacttc tgataaattt ttttgaaaca tcatagaaaa aaccgcacac
     4081 aaaatacctt atcatatgtt acgtttcagt ttatgaccgc aatttttatt tcttcgcacg
     4141 tctgggcctc tcatgacgtc aaatcatgct catcgtgaaa aagttttgga gtatttttgg
     4201 aatttttcaa tcaagtgaaa gtttatgaaa ttaattttcc tgcttttgct ttttggggtt
     4261 tcccctattg tttgtcaaga tttcgaggac ggcgtttttc ttgctaaaat cacaagtatt
     4321 gatgagcacg atgcaagaaa gatcggaaga aggtttgggt ttgaggctca gtggaaggtg
     4381 agtagaagtt gataatttga aagtggagta gtgtctatgg ggtttttgcc ttaaatgaca
     4441 gaatacattc ccaatatacc aaacataact gtttcctact agtcggccgt acgggccctt
     4501 tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
     4561 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
     4621 gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag
     4681 agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag
     4741 gcggccttaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
     4801 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     4861 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     4921 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     4981 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     5041 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     5101 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     5161 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     5221 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     5281 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     5341 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     5401 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     5461 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     5521 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     5581 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     5641 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     5701 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     5761 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     5821 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     5881 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     5941 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     6001 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     6061 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     6121 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     6181 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     6241 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     6301 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     6361 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     6421 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     6481 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     6541 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     6601 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     6661 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     6721 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
     6781 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
     6841 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
     6901 ttccggctcg tatgttgtgt ggaattgtga gcggataaca att
//
