LOCUS       sequence-375997-        7570 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  flp-32 fluorescent neural reporter driving nuclear mTagBFP2 
            expression (refer to NeuroPAL paper for expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7570)
  AUTHORS   Yemini E, Lin A, Nejatbakhsh A, Varol E, Sun R, Mena GE, Samuel ADT,
            Paninski L, Venkatachalam V, Hobert O
  TITLE     NeuroPAL: A Multicolor Atlas for Whole-Brain Neuronal Identification
            in C. elegans.
  JOURNAL   Cell. 2021 Jan 7;184(1):272-288.e11. doi: 
            10.1016/j.cell.2020.12.012. Epub 2020 Dec 29.
  PUBMED    33378642
REFERENCE   2  (bases 1 to 7570)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.012"; journalName: "Cell"; date: "2021-01-7-
            7"; volume: "184"; issue: "1"; pages: "272-288.e11"
COMMENT     Sequence Label: pEY81
FEATURES             Location/Qualifiers
     source          1..7570
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6..22
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2935..2955
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2977..2997
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(5161..5183)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5283..5302
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(5380..5398)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5466..5570
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5571..5639,5640..6431)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5789..5808)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6602..7190
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7091..7110
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7344..7361
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7478..7499
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7514..7519,7520..7537,7538..7544)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7552..7568
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(7557..7570,1..9)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcctgcagg aatcgataat
      181 gtgcgaaggg ggacatagtg gtgaggggag ggtgagagag tgtggaggag tagagaaaaa
      241 gagaagtgta acgatggtgc atctttagac caaaatcata aaaggcaagt aacgatatta
      301 aaatatatga tcccgtttgt ctactattgc attttgtatc ttggttctct ctgattttat
      361 cgaaaaattg ttaattaaca aaatatgtgc aaaatatttt cttttacatt ttgatgttgg
      421 aacatttttt ggaaaatttt agacgaccga gatataacct gctaaagtag aaaaatggag
      481 gtacaagttg tataggaaaa ttacggttat ggaaatataa ctagaaattt ttattctcat
      541 ttaaaattca aagagcttac atataaacaa agtaacatga aaaagcaaag aacatggcaa
      601 aacaatcatg agaatgaggt tttaatgctt gcaaggagaa cccgtaaata tacattctac
      661 cctatatatt ttcccatagt tacattctca caaatagcgg gtttcccaac aagataccga
      721 atttttctaa ttagtagtag tgcatgcaat agtatatttc aattgatatt tttgacccta
      781 ataacagatt tttttgtcgg ttttctaatt taaaaatcgt aataataaaa catattcccc
      841 cccccccccc caatatataa gaactatcgt tccaaaatga actactccca taagctgttc
      901 tcatggtggt tttttttaat caagaagatt aacgcaaaaa tatagtcttc ttccaaatga
      961 ttgtcaaaac atttaaggct cgatcaaacg tcttatacca ataaatattc atataaaatt
     1021 attcctaaat gaataaaccc ctatgatttt ggtaaaaccg caaattgaaa attctcatta
     1081 ctttttgaac ctacccagaa cttggaaaaa tcactgagat gattcatgtt tttgtttgct
     1141 tttatcaaat atgtatagaa atcctctaac cttggttatg tgctcttgag agaatctagg
     1201 atttttttgc ctgcaagctt ctggtttttg gaacacaaat gatattcttt tttgcgaaaa
     1261 gttcactgtt tgtcaggcgt tcttcactta cttaatttat tttttcaaat aatttcataa
     1321 tttaataaat gttggaatgg aatgcgagta tttctcaatt ttgttgtgct tttttgttcc
     1381 tgatcataaa attctacgga aataaaattg gaacggttgt tgttttcttc caatttttga
     1441 gcgcttgaaa gaacacaaac tataagtccg aggcgcatca cagagttcaa gtttttaaaa
     1501 agattttgca tttcagtttt agaccaattt tgctggtttc caaaattgaa taattagact
     1561 tatccaatct gtagtaaaat tgtatttacg gaaaatatta caatgcaagg ccgtaagtat
     1621 attttaaaaa ttgagtacgc ctgtgggact ttttcaaaca tttagtaatt atttattttt
     1681 ttgacttgct cacagtaact catggaggcc aaaaccggtt gaaaaaattt atccatcgta
     1741 aggatgttcg acaaatttca gcagtttaga tcagcaaaca agaatgtttg aatgaaagtt
     1801 tgttggacgt gccaagcaat gcttagacct aggtaacttt cgtgaccatt tttgaagatc
     1861 acctatttta ttttattcac tataatttcc ttttcacata ctacttcgaa attcaaagca
     1921 ttatttttcg tgtggactct gaaccgatcg agaacattac agaagcttat aacacttaag
     1981 tgctcttaaa ataatgttgt ttaccttgca gaaaattcat cgaatacctc ccaacaagta
     2041 tacacctgaa atgtgctcca caattgtctt gttgcacaca aagttccccc gtttccaact
     2101 atcggcaatt cgttgttcct acctcgtcat tttgtcttta aattatcgga acaatggaac
     2161 aaaagaataa gtgagaagtg aaaaagagaa agattcgtct tttttttgcg ctgacactgt
     2221 tgaaaatgta tgagtggggt gtgagttggc ggcgtcgacg aacattgcca agacaaagaa
     2281 acgttggtct gtgtggttct ttggtaaaac attaactttt tggttagtag atagggttag
     2341 gtactcaacc acacaccaag aatgaggagt ttctcctttg ttcacgttca gatttttgat
     2401 cggcttcggc ttcttcgtcc cgcgccgcaa ttagtaatat tctgttctcg acagcttatc
     2461 ccatctaaaa cccggaaaaa gtcgggctca tcctccgagc cggagactcc gacatcactt
     2521 tttggaaaga aattacgtgt tcaaattcag gttttcgaaa tgtaatgcga ttgctgcttc
     2581 atttgttatt cgattatatc gttccgttct ctggctcaat tatggaagtg tcattttggt
     2641 aagtaaccct cccagtacta aacacacact gtcatcccta cgaaagcacg acaaaaacag
     2701 tgcggatgac acatgtgcca gtggcagcta aagtgggagg ggtcttcggg ttgggcaatg
     2761 ggaatctgaa agcagctcgc cgagtgagtg ccacgcccca cgaagccttc attcttacga
     2821 cccgggattg gccaaaggac ccaaaggtat gtttcgaatg atactaacat aacatagaac
     2881 attttcagga ggacccttgc ttggagggta ccgagctcag aaaaaatgac cgctccaaag
     2941 aagaagcgca aggttccagt cgagaagatg actgccccaa aaaagaaacg taaggtcccg
     3001 cggatgtcag agcttattaa ggagaatatg catatgaaac tgtacatgga aggaaccgtg
     3061 gacaaccatc actttaagtg cacttcggag ggggaaggca aaccgtacga gggcacccag
     3121 accatgcgaa tcaaggtggt cgagggaggc ccactgccct tcgccttcga cattctggta
     3181 agtttaaaca tatatatact aactaaccct gattatttaa attttcaggc tactagcttc
     3241 ctgtacggca gcaagacttt tattaatcac acccagggca tcccggactt cttcaaacag
     3301 tcgttccctg agggcttcac atgggagcgt gtgaccacat acgaagacgg tggcgttctc
     3361 accgctaccc aggacaccag cctccaggac ggttgcctca tatataatgt ggtaagttta
     3421 aacagttcgg tactaactaa ccatacatat ttaaattttc agaagatccg gggagtaaac
     3481 ttcacatcca atggtccggt tatgcagaag aaaacactcg gctgggaggc cttcaccgag
     3541 acgctgtatc cggctgacgg aggcctggaa ggtcgaaacg acatggccct gaagctcgtc
     3601 ggcggtagcc atctcatcgc aaacgctaag accacatata gatccaagaa accggtaagt
     3661 ttaaacatga ttttactaac taactaatct gatttaaatt ttcaggctaa gaaccttaaa
     3721 atgccgggag tctactatgt tgactacaga ctggaacgga tcaaggaggc caacaacgag
     3781 acctacgtcg agcagcacga ggttgcagtt gccagatact gcgacctccc gagcaaactg
     3841 ggtcacaagc ttaatcctag gataccggtg ggtgaagacc caccaaagcc atctgccaag
     3901 ggagccaaga aggccgccaa gaccgttacg aagccaaagg acggaaagaa gagacgtcat
     3961 gcccgtaagg aatcatactc cgtctacatc taccgtgtcc tcaagcaagt tcatccagac
     4021 actggagttt cctccaaagc catgtctatc atgaactctt ttgtcaacga tgtcttcgag
     4081 cgtattgctg ctgaagcatc ccgtcttgct cactacaaca agcgttccac aatctcatcc
     4141 cgcgaaattc agaccgctgt ccgtctgatc cttccaggag agcttgccaa gcacgccgtg
     4201 tctgagggaa ccaaggccgt taccaagtac acttccagca agtaggaatt ccaactgagc
     4261 gccggtcgct accattacca acttgtctgg tgtcaaaaat aataggggcc gctgtcatca
     4321 gagtaagttt aaactgagtt ctactaacta acgagtaata tttaaatttt cagcatctcg
     4381 cgcccgtgcc tctgacttct aagtccaatt actcttcaac atccctacat gctctttctc
     4441 cctgtgctcc caccccctat ttttgttatt atcaaaaaac ttctcttaat ttctttgttt
     4501 tttagcttct tttaagtcac ctctaacaat gaaattgtgt agattcaaaa atagaattaa
     4561 ttcgtaataa aaagtcgaaa aaaattgtgc tccctccccc cattaataat aattctatcc
     4621 caaaatctac acaatgttct gtgtacactt cttatgtttt ttacttctga taaatttttt
     4681 tgaaacatca tagaaaaaac cgcacacaaa ataccttatc atatgttacg tttcagttta
     4741 tgaccgcaat ttttatttct tcgcacgtct gggcctctca tgacgtcaaa tcatgctcat
     4801 cgtgaaaaag ttttggagta tttttggaat ttttcaatca agtgaaagtt tatgaaatta
     4861 attttcctgc ttttgctttt tggggtttcc cctattgttt gtcaagattt cgaggacggc
     4921 gtttttcttg ctaaaatcac aagtattgat gagcacgatg caagaaagat cggaagaagg
     4981 tttgggtttg aggctcagtg gaaggtgagt agaagttgat aatttgaaag tggagtagtg
     5041 tctatggggt ttttgcctta aatgacagaa tacattccca atataccaaa cataactgtt
     5101 tcctactagt cggccgtacg ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa
     5161 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag
     5221 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta
     5281 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag
     5341 atgcgtaagg agaaaatacc gcatcaggcg gccttaaggg cctcgtgata cgcctatttt
     5401 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
     5461 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     5521 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     5581 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     5641 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     5701 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     5761 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     5821 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     5881 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     5941 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     6001 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     6061 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     6121 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     6181 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     6241 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     6301 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     6361 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     6421 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     6481 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     6541 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     6601 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     6661 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     6721 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     6781 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     6841 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     6901 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     6961 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     7021 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     7081 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     7141 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     7201 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     7261 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     7321 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
     7381 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
     7441 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
     7501 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
     7561 gataacaatt
//
