LOCUS       sequence-375998-        6524 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  acr-2(2.1k) fluorescent neural reporter driving nuclear mNeptune2.5 
            expression (refer to NeuroPAL paper for expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6524)
  AUTHORS   Yemini E, Lin A, Nejatbakhsh A, Varol E, Sun R, Mena GE, Samuel ADT,
            Paninski L, Venkatachalam V, Hobert O
  TITLE     NeuroPAL: A Multicolor Atlas for Whole-Brain Neuronal Identification
            in C. elegans.
  JOURNAL   Cell. 2021 Jan 7;184(1):272-288.e11. doi: 
            10.1016/j.cell.2020.12.012. Epub 2020 Dec 29.
  PUBMED    33378642
REFERENCE   2  (bases 1 to 6524)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.012"; journalName: "Cell"; date: "2021-01-7-
            7"; volume: "184"; issue: "1"; pages: "272-288.e11"
COMMENT     Sequence Label: pEY39
FEATURES             Location/Qualifiers
     source          1..6524
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6..22
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2199..2219
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2244..2264
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2286..2306
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(4115..4137)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4237..4256
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4334..4352)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4420..4524
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4525..4593,4594..5385)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4743..4762)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5556..6144
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6045..6064
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    6432..6453
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6468..6473,6474..6491,6492..6498)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6506..6522
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(6511..6524,1..9)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcactagtt tcagctcacc
      181 accgactgat tttcatttca aaattaatgc agatttctac gtgttctttt tcaaataaat
      241 ttatatattt tttgttctct cacaaaataa ataatttcga gagttaagtg atcattctca
      301 gacatattct ataattgtcc gtcaggaaac agatagagga acctttgata aataaattat
      361 aaaaggccga cagggaaatg cttctccctc gactctgtta gaagcgaaga ggcaattttt
      421 aaaaatttcc catttttatg cgtttttctg atttccaaag tcacgcatca tttaccaacc
      481 gcaacgattc ataaggacgt aattttgatt ttctggacga tttttatata gttttgatgt
      541 gaaattcgct ttatttgtct gaaagtggaa tagatttagt gtggatttaa ccgaaaaatg
      601 cagatctttt aaaaatttaa accagtgttt tgttgcctta tttgcgaaaa aaaaattgtg
      661 atatttcgag tattgctgca atttgattag taaaatgtta ggatataagt atttaaaaag
      721 tataattggt tgaattttct ttttcatcac cgacttccaa aaatatagag atactgagtt
      781 ttcaccattt tttgaatgta aataaaaaca ttttgaacac ttgtgctggg attcaatcca
      841 aatacattca ctgtcattta atattattat atatcaaaat aaaacgcata actttttctg
      901 ttttttttta aattttttca aaatgtaggt ttggtaagtt aaggtaaggt taggcaatgt
      961 aggtaaacac gagatgatac tccacgttga aaattgtttt cataaataca gtttagattg
     1021 aactgaagga ttttatcgtc taatagaata taatttttta gttcaagatt taggatttgt
     1081 tttcactttt ttttagattc aagacatata tctcgcaaaa tgagttttgt ctttgagcca
     1141 tcttgtcatc atagcacatt gaattttgtt gttcgcaaga cggaatatat gttcccagct
     1201 gcaaattttg tgtcggatat ttcttcttta gcgaaaggcg ttaaaaacca aaaatagtcg
     1261 aagcgtttga tatgtgtccc gttggttttt cgtgtccatc tattctgttt cgagtacaca
     1321 tgttgcgacg cggcactgat gaaaaagtga aagagctagt gtcacacaca aacacaaagt
     1381 atgctcatgt gagtgtgtag atatgtgtag atgtatgtcc tgaaggaata aaaagacttt
     1441 ttgaagctac atttttaaac aaaaaaagac agaaacatga agcacaactc tctattctgt
     1501 cgaacggcgt gtgcaactca cgcacaccca aaaatatgaa atataacatt cgcaccaaca
     1561 aagcgcgaat atcaatgaat ctcgagggaa cgatggctgc ctgccggcgc gtgttgctct
     1621 tctgtcattg ggataactgg cgggtctgtt gggtccgtcc tttagaccat ttgactacac
     1681 tgccctgcat atgtgtgaaa cgtcgtcgtt gctgttggga gctgtctgtg tgtatgtgtg
     1741 tgtgtgtgag tatggagcag taaaaagctg ccgatacgag aagcgccgcc ccggacggac
     1801 ggcgatggcg catcgccaaa tgtgtcattt tagttgtctt cacgaaccag agggcccacg
     1861 acggacgggc ggccacgccc acctcaacca gaatatcctt ccttctccca cccacccttg
     1921 cggacgcgcg gatgcgctga acaaaaagac gacgaagaag aagaagaaga agctgaacga
     1981 agaaaaaagc atcaaaagaa gtgaagcggc tctaccaccc cgaccacctt ccttcacagg
     2041 aaggacgccg ttttcggatc cccgggattg gccaaaggac ccaaaggtat gtttcgaatg
     2101 atactaacat aacatagaac attttcagga ggacccttgg agggtaccgg ttttcccagt
     2161 cacgacgcgt ggtaccgagc tcagaaaaaa tgaccgctcc aaagaagaaa cgcaaagtac
     2221 cgagctcaga aaaaatgacg gcacctaaaa agaaacgcaa agtaccggtc gccgagaaga
     2281 cagccccaaa aaagaagcgt aaagttccac gcgtcatggg agtaagttta aacagttcgg
     2341 tactaactaa ccatacatat ttaaattttc agcgcgctgt ctccaaggga gaggagctca
     2401 tcaaggagaa catgcacacc aagctctaca tggagggaac cgtcaacaac caccacttca
     2461 agtgcaccca cgaaggggag ggcaagccct acgagggcac ccagaccaac aggattaagg
     2521 tggtggaggg aggccccctg ccgttcgcat tcgacatcct ggccacctgc tttatgtacg
     2581 ggagcaagac cttcatcaac cacacccaag gaatcccaga cttcttcaag caatccttcg
     2641 taagtttaaa catgatttta ctaactaact aatctgattt aaattttcag cctgagggct
     2701 tcacatggga gagagtcacc acatacgaag acgggggcgt gcttaccgtt acccaggaca
     2761 ccagcctcca ggacggctgc ttgatctaca acgtcaagct cagaggggtg aacttcccat
     2821 ccaacggccc tgtgatgcag aagaaaacac tcggctggga ggccagtacc gagaccctgt
     2881 accccgctga cggcggcctg gaaggcagat gcgacatggc cctgaagctc gtgggcgggg
     2941 gccacctgca ctgcaacctg aagaccacat acagatccaa gaaacccgct aagaacctca
     3001 agatgccagg agtaagttta aacatatata tactaactaa ccctgattat ttaaattttc
     3061 aggtctactt tgtggaccgc agactggaaa gaatcaagga ggccgacaat gagacctacg
     3121 tcgagcagca cgaggtggct gtggccagat actgcgacct ccctagcaaa ctggggcaca
     3181 aacttaatgg catggacgag ctgtacaagt aagcgccggt cgctaccatt accaacttgt
     3241 ctggtgtcaa aaataatagg ggccgctgtc atcagagtaa gtttaaactg agttctacta
     3301 actaacgagt aatatttaaa ttttcagcat ctcgcgcccg tgcctctgac ttctaagtcc
     3361 aattactctt caacatccct acatgctctt tctccctgtg ctcccacccc ctatttttgt
     3421 tattatcaaa aaacttctct taatttcttt gttttttagc ttcttttaag tcacctctaa
     3481 caatgaaatt gtgtagattc aaaaatagaa ttaattcgta ataaaaagtc gaaaaaaatt
     3541 gtgctccctc cccccattaa taataattct atcccaaaat ctacacaatg ttctgtgtac
     3601 acttcttatg ttttttactt ctgataaatt tttttgaaac atcatagaaa aaaccgcaca
     3661 caaaatacct tatcatatgt tacgtttcag tttatgaccg caatttttat ttcttcgcac
     3721 gtctgggcct ctcatgacgt caaatcatgc tcatcgtgaa aaagttttgg agtatttttg
     3781 gaatttttca atcaagtgaa agtttatgaa attaattttc ctgcttttgc tttttggggt
     3841 ttcccctatt gtttgtcaag atttcgagga cggcgttttt cttgctaaaa tcacaagtat
     3901 tgatgagcac gatgcaagaa agatcggaag aaggtttggg tttgaggctc agtggaaggt
     3961 gagtagaagt tgataatttg aaagtggagt agtgtctatg gggtttttgc cttaaatgac
     4021 agaatacatt cccaatatac caaacataac tgtttcctac tagtcggccg tacgggccct
     4081 ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga
     4141 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
     4201 cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga
     4261 gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca
     4321 ggcggcctta agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
     4381 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     4441 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     4501 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     4561 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     4621 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     4681 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     4741 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     4801 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     4861 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     4921 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     4981 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     5041 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     5101 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     5161 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     5221 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     5281 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     5341 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     5401 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     5461 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     5521 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     5581 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     5641 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     5701 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     5761 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     5821 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     5881 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     5941 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     6001 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     6061 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     6121 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     6181 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     6241 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     6301 gagtcagtga gcgatgaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
     6361 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
     6421 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
     6481 cttccggctc gtatgttgtg tggaattgtg agcggataac aatt
//
