LOCUS       sequence-376001-        8341 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ceh-14 fluorescent neural reporter driving nuclear mNeptune2.5 
            expression (refer to NeuroPAL paper for expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8341)
  AUTHORS   Yemini E, Lin A, Nejatbakhsh A, Varol E, Sun R, Mena GE, Samuel ADT,
            Paninski L, Venkatachalam V, Hobert O
  TITLE     NeuroPAL: A Multicolor Atlas for Whole-Brain Neuronal Identification
            in C. elegans.
  JOURNAL   Cell. 2021 Jan 7;184(1):272-288.e11. doi: 
            10.1016/j.cell.2020.12.012. Epub 2020 Dec 29.
  PUBMED    33378642
REFERENCE   2  (bases 1 to 8341)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.012"; journalName: "Cell"; date: "2021-01-7-
            7"; volume: "184"; issue: "1"; pages: "272-288.e11"
COMMENT     Sequence Label: pEY68
FEATURES             Location/Qualifiers
     source          1..8341
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6..22
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             4016..4036
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4061..4081
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4103..4123
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(5932..5954)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6054..6073
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6151..6169)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6237..6341
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6342..6410,6411..7202)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6560..6579)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7373..7961
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7862..7881
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    8249..8270
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8285..8290,8291..8308,8309..8315)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8323..8339
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(8328..8341,1..9)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcctgcagt ggtggtggtt
      181 atggaaagaa ataaatccct ttctctcttt ttttttgaaa tgatttattg atgattggta
      241 gtcaccgaaa aaccaatatc aatgttaaag caactatcgt agtataattc aagccccgtt
      301 taattttgtt ttgccatttc aatcattcca ctcacgtcgt tacagattta ttaatgtgct
      361 tgcttgtgta ataaatcatt tcttcgaaaa ctaattttga gtatctgaca agaaataaag
      421 catacatgtc tcagactcgg tacaacccat acgcatagac acatgaagca aaagccaaaa
      481 ggtattgaat aggaaattca agggttaacg gccacggtcg taaactcgaa gacataaaac
      541 aaggagaaga aggcgactgg aaaggcagct ggctgtcagg gcagacaaca tccacatgac
      601 tctcgcgcac ataccacaga ggataaagat aggagatccc tgagaatatc gttctgacta
      661 ctgtaatgac ctgcagcaac gaggggtgtt aagtaaaatg agcttaatta tatgtttcga
      721 ccattagtgg gttctaccca tgaaaatagc ttatgatatg ggttatgact tctgtggagt
      781 tcttttacac tataaagaaa acaacattat ataacacaaa attaaaccat tctgatacat
      841 tttcaatgac aagatgtgat gctttcttgt cttttgacgc gtcccgcgtg tataattcag
      901 tttcctgaca agtcataagt tgtaaatatt ttatgattgt tcaccatgta ttccatttcc
      961 agaagctagg aaaaaacaca actcaagact gtttgtttcc attcttatct tgaaaaaata
     1021 acgttggtat gaaatttggg acatcgtaaa aaatggtata attttaaaaa aacggtcttt
     1081 taaatgctaa tatagtgtac gtgagaaaat aactttctga agtatctact caacgtgtta
     1141 ttttccaaaa aaattgcatt aagttaaagc tgattaatat ttagtttttt ttaatttggt
     1201 ttacatgttt acaaaagtca agagccaaaa aatccaaatt gtaaagaaag tcttttctgc
     1261 aaaactgtat acatcaatgt ctgagatgca gatataaaat agtataaaca aaaatggagt
     1321 aacgtcagta agtgatatta ttgaagctgg aaaactgagg taactttact tttcgagaaa
     1381 tcttaggcac ttccgaacgc ctgaaatgtt ggaatataaa tattttaaac atcattagaa
     1441 caaaagtttg aaagaaagct ttttgatcga tcattttcaa aaaaagtcgg aaagtgagtt
     1501 tcccccgttt tttgattaga aataaaatat acttacattt tttgaaaagt gttatcatga
     1561 tatgcagtca ttttggcgca aaggaaatct ttaatcattt cctccacatt tttacatttt
     1621 taagtaaaaa ttaaaattgg gaatttttaa atcatttgat gaaaactgta tcgcaaaata
     1681 ttttttgact ttttacacta agaaattttt atatttgtaa ctgttaaact gttcatgcta
     1741 gctgatgccg attgaaaaca aactgattca acacattggg tgccgggcac ggtcaatcgg
     1801 tttacaaaat attttacaat gcggaaatca aatgctcaca tctttatgct aaatggttca
     1861 gtcatgaacc atgaagaaaa agaatgaatc agaaaaaact taagttttac aacaagacca
     1921 acagttttcc ctcactttcc tgtctcctca cgaattgatt ctccttccgg ctgcccagtc
     1981 actctccata acttaattga tacattttag caccaatttt atgacgagca gaatttgttg
     2041 cgaacatgtt gtcagctgcc cttctaaaat acatccatgc ctcccctccc ctcaaccatt
     2101 accgatcatt gtccgtatac gcgcaccata ttggcgcgtt gatggacgaa ggcagagagc
     2161 agcagcacga cgacgtcatt atatcaagac tggtggcagc ttttcgggac gagagccgag
     2221 acccagctat tacctaattt tgaagcgctt ctatcctggc ctgcaagccg gataatggat
     2281 gtgctctcgc acgttgtctt gtctgctttt gagagcagca ttggtttata ttacttttaa
     2341 ttaactattt cgtaaacttc tgatcttttg ttaagcagac aaattatcaa tgttttctta
     2401 ggttggctta aagcttggga taggcaccat acatttccaa aaaagaagta ctaaagcagt
     2461 ctgcagatcg tgaacctata ataatttagt ctagacaaat acaagcaata tactatcaca
     2521 tattattgta atgatagaag tagtgaaaaa gaaactcaca cagaaattgc tataaaaatg
     2581 atcaaccgga acattaccaa cttacattgt ttcatgttta tttttgttat ttattaagta
     2641 aaataattta taagaacttt ttctagtagt cttgtaccac aattgttgaa cattgaaagc
     2701 tcaaagttgt tgctttgtcg gaacattttc agtcggaaca agtgccaaat atgtgtgctt
     2761 ttgatattcc aatagtttaa caaatttgaa tagaatccaa aactcaagat atgagttgcc
     2821 acataagagc agtaaagatt taagcttaat agaaacaata ctaatacggt aatacgtata
     2881 tttgacgata atttttgaat agacattttg aagctatttt tgtattttta aaacttccat
     2941 gaaattcaat catcgggtta ctttactaaa tagtttatat tttacttatt catagtttcg
     3001 actgttttgt attttaagat caatgtttac tactgttagg ctcctcaagt tgaatttctg
     3061 ttaattctat tgtttccaag atgatatttc attctgaaac tctactcctg aaaattcaac
     3121 aattgttttc atttaaaatg agcaactgta attttctatt cattaaagta ttttttttac
     3181 catttaaaag gaacccattc atgaaaagtt caatagtttc taataatgca ataatccaca
     3241 aaaacatgtt catgccgcaa tttaattttt tcttgaccta actgatcatt tcattttctc
     3301 atttcatttc aaaaagcacg caatattaat gaaactagga caccctctct taattgcttt
     3361 tgacatcaat taagttgaaa tgtttctttc ttttttcaaa ttttgcactt tttcaatcat
     3421 tttgaaagta gaatataaac aaagtttttt ggaaagatgc tattgctccg aaaatcacga
     3481 ggaacgagcg gatttgagag gatttctgaa tttatcgatg ataaaaacaa tcttcttatt
     3541 ttcgcggccg tttctgtaaa acatttgctt agatgtgata agtcatttga gcaacattca
     3601 attagtgaac tttaggtttg aatccattgt ttcgggagat gtatcgtatc ccagagctaa
     3661 gaaatgcaca agagagcatg gcttcttacc cgctcattgg cgtgagacaa gaaatgggcg
     3721 ggactttatt ttcgggacat tcatggctcg tggagcataa catacacgta gtctcaagaa
     3781 ccgactaacg acattttttc ttaactttcc atacgagttt gatcatgagt ctgaaacatt
     3841 tgactaggct ttttataaaa gttcattagc tattcaaccc gggattggcc aaaggaccca
     3901 aaggtatgtt tcgaatgata ctaacataac atagaacatt ttcaggagga cccttggagg
     3961 gtaccggttt tcccagtcac gacgcgtggt accgagctca gaaaaaatga ccgctccaaa
     4021 gaagaaacgc aaagtaccga gctcagaaaa aatgacggca cctaaaaaga aacgcaaagt
     4081 accggtcgcc gagaagacag ccccaaaaaa gaagcgtaaa gttccacgcg tcatgggagt
     4141 aagtttaaac agttcggtac taactaacca tacatattta aattttcagc gcgctgtctc
     4201 caagggagag gagctcatca aggagaacat gcacaccaag ctctacatgg agggaaccgt
     4261 caacaaccac cacttcaagt gcacccacga aggggagggc aagccctacg agggcaccca
     4321 gaccaacagg attaaggtgg tggagggagg ccccctgccg ttcgcattcg acatcctggc
     4381 cacctgcttt atgtacggga gcaagacctt catcaaccac acccaaggaa tcccagactt
     4441 cttcaagcaa tccttcgtaa gtttaaacat gattttacta actaactaat ctgatttaaa
     4501 ttttcagcct gagggcttca catgggagag agtcaccaca tacgaagacg ggggcgtgct
     4561 taccgttacc caggacacca gcctccagga cggctgcttg atctacaacg tcaagctcag
     4621 aggggtgaac ttcccatcca acggccctgt gatgcagaag aaaacactcg gctgggaggc
     4681 cagtaccgag accctgtacc ccgctgacgg cggcctggaa ggcagatgcg acatggccct
     4741 gaagctcgtg ggcgggggcc acctgcactg caacctgaag accacataca gatccaagaa
     4801 acccgctaag aacctcaaga tgccaggagt aagtttaaac atatatatac taactaaccc
     4861 tgattattta aattttcagg tctactttgt ggaccgcaga ctggaaagaa tcaaggaggc
     4921 cgacaatgag acctacgtcg agcagcacga ggtggctgtg gccagatact gcgacctccc
     4981 tagcaaactg gggcacaaac ttaatggcat ggacgagctg tacaagtaag cgccggtcgc
     5041 taccattacc aacttgtctg gtgtcaaaaa taataggggc cgctgtcatc agagtaagtt
     5101 taaactgagt tctactaact aacgagtaat atttaaattt tcagcatctc gcgcccgtgc
     5161 ctctgacttc taagtccaat tactcttcaa catccctaca tgctctttct ccctgtgctc
     5221 ccacccccta tttttgttat tatcaaaaaa cttctcttaa tttctttgtt ttttagcttc
     5281 ttttaagtca cctctaacaa tgaaattgtg tagattcaaa aatagaatta attcgtaata
     5341 aaaagtcgaa aaaaattgtg ctccctcccc ccattaataa taattctatc ccaaaatcta
     5401 cacaatgttc tgtgtacact tcttatgttt tttacttctg ataaattttt ttgaaacatc
     5461 atagaaaaaa ccgcacacaa aataccttat catatgttac gtttcagttt atgaccgcaa
     5521 tttttatttc ttcgcacgtc tgggcctctc atgacgtcaa atcatgctca tcgtgaaaaa
     5581 gttttggagt atttttggaa tttttcaatc aagtgaaagt ttatgaaatt aattttcctg
     5641 cttttgcttt ttggggtttc ccctattgtt tgtcaagatt tcgaggacgg cgtttttctt
     5701 gctaaaatca caagtattga tgagcacgat gcaagaaaga tcggaagaag gtttgggttt
     5761 gaggctcagt ggaaggtgag tagaagttga taatttgaaa gtggagtagt gtctatgggg
     5821 tttttgcctt aaatgacaga atacattccc aatataccaa acataactgt ttcctactag
     5881 tcggccgtac gggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca
     5941 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc
     6001 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc
     6061 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag
     6121 gagaaaatac cgcatcaggc ggccttaagg gcctcgtgat acgcctattt ttataggtta
     6181 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg
     6241 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     6301 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     6361 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     6421 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     6481 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     6541 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     6601 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     6661 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     6721 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     6781 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     6841 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     6901 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     6961 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     7021 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     7081 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     7141 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     7201 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     7261 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     7321 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     7381 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     7441 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     7501 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     7561 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     7621 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     7681 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     7741 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     7801 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     7861 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     7921 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     7981 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     8041 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     8101 gaacgaccga gcgcagcgag tcagtgagcg atgaagcgga agagcgccca atacgcaaac
     8161 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
     8221 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
     8281 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
     8341 t
//
