LOCUS       sequence-376004-        6853 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  unc-8 fluorescent neural reporter driving nuclear CYOFP expression 
            (refer to NeuroPAL paper for expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6853)
  AUTHORS   Yemini E, Lin A, Nejatbakhsh A, Varol E, Sun R, Mena GE, Samuel ADT,
            Paninski L, Venkatachalam V, Hobert O
  TITLE     NeuroPAL: A Multicolor Atlas for Whole-Brain Neuronal Identification
            in C. elegans.
  JOURNAL   Cell. 2021 Jan 7;184(1):272-288.e11. doi: 
            10.1016/j.cell.2020.12.012. Epub 2020 Dec 29.
  PUBMED    33378642
REFERENCE   2  (bases 1 to 6853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.012"; journalName: "Cell"; date: "2021-01-7-
            7"; volume: "184"; issue: "1"; pages: "272-288.e11"
COMMENT     Sequence Label: pEY38
FEATURES             Location/Qualifiers
     source          1..6853
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6..22
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2212..2232
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2254..2274
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(4444..4466)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4566..4585
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4663..4681)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4749..4853
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4854..4922,4923..5714)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5072..5091)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5885..6473
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6374..6393
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6627..6644
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6761..6782
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6797..6802,6803..6820,6821..6827)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6835..6851
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(6840..6853,1..9)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcctgcagc ttaaggagca
      181 tttccggtag tttgaacttc taaaaactac acttttcgat cttttatttt caaagtctct
      241 tctgaatttc tgtagtaaga agatgtccaa cagagacgtc aacaatatac atctaattgc
      301 ggatgggctc tcagagcaac aaaaagatag atgactctct tccaatcccc tcgtctactt
      361 ttcgtctctt gtcaaaattt tgaatccgtt agtctgtgca ctttatgatt ccaaaatata
      421 catttccacg tcgcaaggat gcaattcggc acaaaattcg aaaaaatgtc acctttgctg
      481 acgtggaact tgatatgtgt caggtaactt ctattttcag tcatactatt ggtaaatgta
      541 aattcttttt ccaaacaata accaaaaatt atcccaaaac caataaccac ttttaatcca
      601 aaaataggaa ccttggtaat aaatttccca attgggagtt ccccgaagtg aagctaatcg
      661 cagattcgtg taaaaccccc gtcttttgac aaaaaaccaa gaaggaaatc gcggcaagac
      721 caaaaaaaga atgaagagta atacgacatg caatgatccc aagagaggcc aattgtcttt
      781 cagttgatcc ctcaggaaga ggactttcct ttttcaaaat gtgtcccaag cttttgtttc
      841 atgtcacaca atttttgaag ttgacactat tttcagttca aggtggtaca caattttatg
      901 tttaaaaaat aaagttaagt tctggttagg aacagtatta atttttgtta tagaattgaa
      961 tttaaaacat ctttaaaatt atttaaattg tcgtttattt tgttttcaga gcaacacatt
     1021 tctacattta ttgaatctat ctgtttcaaa attttaataa ttcacactat tattttagag
     1081 taatagataa agtttaaacc ctgaatttat ttgactatta ttttttatag ctcccgcctt
     1141 tttttcgttt aatacataca attttattaa aatgaaccct gtttgatgaa ttcttctcta
     1201 ctcggtcctt tatgaaatta gcatatcctc ctgatccacc caaaagaatg tcaactgaat
     1261 aataggagaa aaagataaaa gagagtagat ttgaacattt gaaaccaaga ttcccattta
     1321 gataagctta tattgtgggg actccggggc cacacggcgt cctaatacaa gcttttcaag
     1381 agcttgctct cattttttct caacaatatt gtttagtatc ttcaaaagtt ttgcataagc
     1441 atgctacagt tgaagacatt ccagcttttc actatgaaat atttccttca tatttttatt
     1501 gcagagactc gtccatgaac cagaatcaat ggagagcagg tcaagtccat atattagacc
     1561 atctccatac gctggaggag ttcatccaca ctttgaagag gaagatgaca ggtcaaaagt
     1621 gagttttatt caaagctacg ttttcaaact tttgtttcat tttttcgttc ttaccgagat
     1681 ttttttgatt caaaattgcg ggttgtcatc gaaataagaa aggaaaatac aacagatatt
     1741 aagttttgct tcgcctattc tttaaaaaaa attatcgttg ttattttgag cattaggaag
     1801 atgttatgaa tcaaaagatg ttacattttt gagcattatc agaatgtcgt ccatttctca
     1861 ttttactagt ggaacacgac gcaattcgtt cttaaaagta taactttaca catatttttc
     1921 tattaaatta tttttgtcat gtttctaata tacgaagttg ccaaggtttt cagtggttta
     1981 tttagtaaat ggggccctaa taatttcgaa tcttcggtga tcacaataaa attgttttca
     2041 aaaatatagg atcaatgaaa attattttac tattgtatac tctttgcttg aatttgaatg
     2101 atgtttccaa aacatttcaa ctcatttttt cagcttcatg catcagctct ctactcggaa
     2161 cgccgaacgt catcccggaa atcggtaccg agctcagaaa aaatgaccgc tccaaagaag
     2221 aagcgcaagg ttccagtcga gaagatgact gccccaaaaa agaaacgtaa ggtcccgcgg
     2281 gtaagtttaa acagttcggt actaactaac catacatatt taaattttca gcgcgctgtg
     2341 tcaaaaggag aagagcttat taaggagaat atgcgttcca agctctacct cgagggatcc
     2401 gtcaacggac accaattcaa gtgcacccac gagggagagg gaaagccata cgagggaaag
     2461 caaaccaacc gtatcaaggt cgtcgaggga ggaccactcc cattcgcctt cgacatcctc
     2521 gccacccact tcatgtacgg atccaaggta agtttaaaca tatatatact aactaaccct
     2581 gattatttaa attttcaggt cttcatcaag tacccagccg acctcccaga ctacttcaag
     2641 caatccttcc cagagggatt cacctgggag cgtgtcatgg tcttcgagga cggaggagtc
     2701 ctcaccgcca cccaagacac ctccctccaa gacggagagc tcatctacaa cgtcaaggtc
     2761 cgtggagtca acttcccagc caacggacca gtcatgcaaa agaagaccct cggatgggag
     2821 ccatccaccg agaccatgta cccagccgac ggaggactcg agggacgttg cgacaaggta
     2881 agtttaaaca tgattttact aactaactaa tctgatttaa attttcaggc cctcaagctc
     2941 gtcggaggag gacacctcca cgtcaacttc aagaccacct acaagtccaa gaagccagtc
     3001 aagatgccag gagtccacta cgtcgaccgt cgtctcgagc gtatcaagga ggccgacaac
     3061 gagacctacg tcgagcaata cgagcacgcc gtcgcccgtt actccaacct cggaggagga
     3121 atggacgagc tctacaagcc taggataccg gtgggtgaag acccaccaaa gccatctgcc
     3181 aagggagcca agaaggccgc caagaccgtt acgaagccaa aggacggaaa gaagagacgt
     3241 catgcccgta aggaatcata ctccgtctac atctaccgtg tcctcaagca agttcatcca
     3301 gacactggag tttcctccaa agccatgtct atcatgaact cttttgtcaa cgatgtcttc
     3361 gagcgtattg ctgctgaagc atcccgtctt gctcactaca acaagcgttc cacaatctca
     3421 tcccgcgaaa ttcagaccgc tgtccgtctg atccttccag gagagcttgc caagcacgcc
     3481 gtgtctgagg gaaccaaggc cgttaccaag tacacttcca gcaagtagga attccaactg
     3541 agcgccggtc gctaccatta ccaacttgtc tggtgtcaaa aataataggg gccgctgtca
     3601 tcagagtaag tttaaactga gttctactaa ctaacgagta atatttaaat tttcagcatc
     3661 tcgcgcccgt gcctctgact tctaagtcca attactcttc aacatcccta catgctcttt
     3721 ctccctgtgc tcccaccccc tatttttgtt attatcaaaa aacttctctt aatttctttg
     3781 ttttttagct tcttttaagt cacctctaac aatgaaattg tgtagattca aaaatagaat
     3841 taattcgtaa taaaaagtcg aaaaaaattg tgctccctcc ccccattaat aataattcta
     3901 tcccaaaatc tacacaatgt tctgtgtaca cttcttatgt tttttacttc tgataaattt
     3961 ttttgaaaca tcatagaaaa aaccgcacac aaaatacctt atcatatgtt acgtttcagt
     4021 ttatgaccgc aatttttatt tcttcgcacg tctgggcctc tcatgacgtc aaatcatgct
     4081 catcgtgaaa aagttttgga gtatttttgg aatttttcaa tcaagtgaaa gtttatgaaa
     4141 ttaattttcc tgcttttgct ttttggggtt tcccctattg tttgtcaaga tttcgaggac
     4201 ggcgtttttc ttgctaaaat cacaagtatt gatgagcacg atgcaagaaa gatcggaaga
     4261 aggtttgggt ttgaggctca gtggaaggtg agtagaagtt gataatttga aagtggagta
     4321 gtgtctatgg ggtttttgcc ttaaatgaca gaatacattc ccaatatacc aaacataact
     4381 gtttcctact agtcggccgt acgggccctt tcgtctcgcg cgtttcggtg atgacggtga
     4441 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
     4501 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa
     4561 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca
     4621 cagatgcgta aggagaaaat accgcatcag gcggccttaa gggcctcgtg atacgcctat
     4681 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
     4741 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     4801 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     4861 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     4921 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     4981 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     5041 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     5101 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     5161 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     5221 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     5281 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     5341 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     5401 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     5461 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     5521 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     5581 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     5641 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     5701 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     5761 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     5821 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     5881 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     5941 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     6001 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     6061 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     6121 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     6181 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     6241 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     6301 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     6361 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     6421 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     6481 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     6541 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     6601 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     6661 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
     6721 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
     6781 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
     6841 gcggataaca att
//
