LOCUS       sequence-376014-        7751 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  flp-18(AVA = 4.2-1k) fluorescent neural reporter driving nuclear 
            mNeptune2.5 expression (refer to NeuroPAL paper for expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7751)
  AUTHORS   Yemini E, Lin A, Nejatbakhsh A, Varol E, Sun R, Mena GE, Samuel ADT,
            Paninski L, Venkatachalam V, Hobert O
  TITLE     NeuroPAL: A Multicolor Atlas for Whole-Brain Neuronal Identification
            in C. elegans.
  JOURNAL   Cell. 2021 Jan 7;184(1):272-288.e11. doi: 
            10.1016/j.cell.2020.12.012. Epub 2020 Dec 29.
  PUBMED    33378642
REFERENCE   2  (bases 1 to 7751)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.012"; journalName: "Cell"; date: "2021-01-7-
            7"; volume: "184"; issue: "1"; pages: "272-288.e11"
COMMENT     Sequence Label: pEY80
FEATURES             Location/Qualifiers
     source          1..7751
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6..22
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             complement(3207..3218)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             3426..3446
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3471..3491
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3513..3533
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(5342..5364)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5464..5483
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(5561..5579)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5647..5751
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5752..5820,5821..6612)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5970..5989)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6783..7371
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7272..7291
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    7659..7680
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7695..7700,7701..7718,7719..7725)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7733..7749
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(7738..7751,1..9)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcctgcaga aatctgtcac
      181 atactgctcg aatcggaggt aggtttgaaa aacatgcaat tcatttccaa aatatgacga
      241 attttgaaaa aaaaaaatag aaaagaaact caattaaatt accttatttt ttctagtacg
      301 tcaattttga cttgcactta aaatctgcat tttaaaaatg aaaattcagt gcagctgcag
      361 tgcagtgcgg ctattatttt cggcattttt gcaaaactta cacaccaccg ttataagaaa
      421 atatttgtct ttttttttaa atttttattc agtcgttatt cgaaaaagac ttacagtacc
      481 ttgctgaatt ccttccgcat tgcgctaaaa tttaccgatt gcaaaataat cttacatgct
      541 atagtaataa aaaatttaca tacttgccat ttcatttcat ttaattattt catccaattt
      601 atcttgatca gtattactct ttgttaaaaa ttccactgtt caatttttac atttcagttg
      661 ttttaatatc gaagtgcaca agaagcgcga acataatgga caccatcgct caaaaattat
      721 gcttctagtt gtagttcctc gagcattttt gatctcaaaa gacatttcca agagcaataa
      781 ttgccatttt tgagctacaa gtaccatatc ctgctcataa ttcatgttcc accactccct
      841 aaagtttttt ctttggaatt ccccagactt tgcgcctaaa attctcgtta ttctctccaa
      901 ttcaattggc tcaattcaac tgactagatt tcagtgatgt aggtagcgcg gaactcagta
      961 ttaagagagg actcaactca gtttctaatc caatttcaac aaattctaag acaccgaaac
     1021 gctgcaggtc tcgtagagcc tacgtgttga caggttcttc cttttcaatc tatttccgtc
     1081 gatattccgg cgtcatttag ttgaaaatga gtgaaaaaat gcgatctgac gcgaattcac
     1141 gtgaactcga aaattcgaga atgtaatcat taagtttcca aacaccaagt tctctttcga
     1201 aaccaagtca atagaaaaat gagacaccca cgagaagaag gttggtccga aaaaagaaac
     1261 taaagaagtt atttatttct ctttttgatg tttgttgagc agaatatgat ttagttaagc
     1321 acatctgatt tcaacgccga tgaatgaggt cgtaggcgtc acgttcaatt tgatctgatt
     1381 tgttcagtgc ttacgagtag aaattctaaa tatgatgtta ctttttggtt ctgtaagatg
     1441 gagctgggta tacatgtggt gcacggcaat cgctatttgg caatttcagt catttcggca
     1501 aattcggcaa ttttggcgaa ttctcatatt ttggaaattt tggcaaatgc ggaaaatttc
     1561 aaaaacaagc aattaccaaa tttgccaaac gccgaaaaat ttaaaaaaaa aaccgacatt
     1621 tgctggtatt tttttttaat caggatgcta attatgtaag gttaatctat aaattcttga
     1681 aacacactta actttgaaat tgaaatcact gacatttcgc gcattcgccg ataaaatctc
     1741 taaataaaat aatgtaaact gtgtccagtt ttggtacttg tttagtatac ctggttcaaa
     1801 atattttcca atgcgttttc catttattta ttttagaaat gatcatttaa ttaaaacgta
     1861 aaattcgcat tttcaagctt agaaacatta atcctaagtc caatatggac aaaaagagta
     1921 gtgtattagt accaagtaag aaccctaaaa aaaaaatatt ctaattaaat ttttggcaaa
     1981 atgccacgtt tttctttttt ttctcaacgg ggaaaagttg tgtttcttcc ctttttatta
     2041 aattttttgt tttccttagg ctaagtacaa cttgaaatgg aacagaaatt acataaattt
     2101 attgctttca cattttgctc ttcttcactc aaatcttgga ctcttgcttt tcacaaaaaa
     2161 aaccttataa acaaaaataa gacatctgaa aaaatgatga acttcctgtc agtttgttcc
     2221 agtatccttc ttaaatttcc ttgttttcca catcacaaaa atcctacatc acgcttcgac
     2281 tcatttcctg acagtcatca catcaccaaa tgaatttcta tttttacaat atagttgtag
     2341 tatttttgtc gaacatatat tgttctaact aacttcttaa aaaattgtgc tcttgactga
     2401 acacaaattt ctgcgtcgtt tcacgtttac ttttctcaat tttttaaaac caaaatttaa
     2461 ctgcaaactt ctgcattgtt ctaaaaactc acaggttatc tcaaaaagat aaatttcaaa
     2521 gtgagatttt tttaaaaaaa ttccggactg ttttttgatt ttgatgtttt taatgaattt
     2581 ttaaaattct ttattagtgc ctgaaattaa caataatttt aaagtttgtc acactgcgaa
     2641 taaattaatc tcacatttcg tatgtttaga aagatgcatg aaactccgtc gttcttatga
     2701 agttaattaa gacatggcta attgaaagcc cacatggtca tgaaaaagta aaacttacaa
     2761 ctttttactg aatattaacc agtctctata aatatcccag cttagccgga atagggtcac
     2821 gaaaacatga aaataaagct tgaacctatt gttatgattt tcaatgccac cttttgcagt
     2881 ttaatataca ctattactac ttctcagttc aagttcactt ctgagacgga acctttattt
     2941 tctaactttc tttaatccta ctttttatga gccgaatgtt ctattttgta aaatgatcag
     3001 atacaagccc aaagctaatt ttgccaataa gtgttagtag atcaattgaa ttacaatata
     3061 atgacctggg cggaatagat ttttgcactg atttttttcc aagcttactc tatgaatatt
     3121 catagatcat cttcaaaggt agcgtgtgtt gtagttgtgt tttggcgggt tcgtgttatt
     3181 tgactcagct caagtagatg ttgtagtctt ccctcaatca acgcgtgggc ttgaccctct
     3241 ttaaattgag tggaaaccag ccaatctatt tgaaatctac ccccgaaccc gggattggcc
     3301 aaaggaccca aaggtatgtt tcgaatgata ctaacataac atagaacatt ttcaggagga
     3361 cccttggagg gtaccggttt tcccagtcac gacgcgtggt accgagctca gaaaaaatga
     3421 ccgctccaaa gaagaaacgc aaagtaccga gctcagaaaa aatgacggca cctaaaaaga
     3481 aacgcaaagt accggtcgcc gagaagacag ccccaaaaaa gaagcgtaaa gttccacgcg
     3541 tcatgggagt aagtttaaac agttcggtac taactaacca tacatattta aattttcagc
     3601 gcgctgtctc caagggagag gagctcatca aggagaacat gcacaccaag ctctacatgg
     3661 agggaaccgt caacaaccac cacttcaagt gcacccacga aggggagggc aagccctacg
     3721 agggcaccca gaccaacagg attaaggtgg tggagggagg ccccctgccg ttcgcattcg
     3781 acatcctggc cacctgcttt atgtacggga gcaagacctt catcaaccac acccaaggaa
     3841 tcccagactt cttcaagcaa tccttcgtaa gtttaaacat gattttacta actaactaat
     3901 ctgatttaaa ttttcagcct gagggcttca catgggagag agtcaccaca tacgaagacg
     3961 ggggcgtgct taccgttacc caggacacca gcctccagga cggctgcttg atctacaacg
     4021 tcaagctcag aggggtgaac ttcccatcca acggccctgt gatgcagaag aaaacactcg
     4081 gctgggaggc cagtaccgag accctgtacc ccgctgacgg cggcctggaa ggcagatgcg
     4141 acatggccct gaagctcgtg ggcgggggcc acctgcactg caacctgaag accacataca
     4201 gatccaagaa acccgctaag aacctcaaga tgccaggagt aagtttaaac atatatatac
     4261 taactaaccc tgattattta aattttcagg tctactttgt ggaccgcaga ctggaaagaa
     4321 tcaaggaggc cgacaatgag acctacgtcg agcagcacga ggtggctgtg gccagatact
     4381 gcgacctccc tagcaaactg gggcacaaac ttaatggcat ggacgagctg tacaagtaag
     4441 cgccggtcgc taccattacc aacttgtctg gtgtcaaaaa taataggggc cgctgtcatc
     4501 agagtaagtt taaactgagt tctactaact aacgagtaat atttaaattt tcagcatctc
     4561 gcgcccgtgc ctctgacttc taagtccaat tactcttcaa catccctaca tgctctttct
     4621 ccctgtgctc ccacccccta tttttgttat tatcaaaaaa cttctcttaa tttctttgtt
     4681 ttttagcttc ttttaagtca cctctaacaa tgaaattgtg tagattcaaa aatagaatta
     4741 attcgtaata aaaagtcgaa aaaaattgtg ctccctcccc ccattaataa taattctatc
     4801 ccaaaatcta cacaatgttc tgtgtacact tcttatgttt tttacttctg ataaattttt
     4861 ttgaaacatc atagaaaaaa ccgcacacaa aataccttat catatgttac gtttcagttt
     4921 atgaccgcaa tttttatttc ttcgcacgtc tgggcctctc atgacgtcaa atcatgctca
     4981 tcgtgaaaaa gttttggagt atttttggaa tttttcaatc aagtgaaagt ttatgaaatt
     5041 aattttcctg cttttgcttt ttggggtttc ccctattgtt tgtcaagatt tcgaggacgg
     5101 cgtttttctt gctaaaatca caagtattga tgagcacgat gcaagaaaga tcggaagaag
     5161 gtttgggttt gaggctcagt ggaaggtgag tagaagttga taatttgaaa gtggagtagt
     5221 gtctatgggg tttttgcctt aaatgacaga atacattccc aatataccaa acataactgt
     5281 ttcctactag tcggccgtac gggccctttc gtctcgcgcg tttcggtgat gacggtgaaa
     5341 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
     5401 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact
     5461 atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca
     5521 gatgcgtaag gagaaaatac cgcatcaggc ggccttaagg gcctcgtgat acgcctattt
     5581 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
     5641 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     5701 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     5761 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     5821 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     5881 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     5941 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     6001 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     6061 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     6121 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     6181 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     6241 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     6301 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     6361 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     6421 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     6481 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     6541 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     6601 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     6661 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     6721 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     6781 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     6841 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     6901 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     6961 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     7021 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     7081 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     7141 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     7201 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     7261 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     7321 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     7381 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     7441 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     7501 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg atgaagcgga agagcgccca
     7561 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
     7621 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat
     7681 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
     7741 ggataacaat t
//
