LOCUS       sequence-376015-       10780 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For use in RBP tethering screen.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10780)
  AUTHORS   Luo EC, Nathanson JL, Tan FE, Schwartz JL, Schmok JC, Shankar A, 
            Markmiller S, Yee BA, Sathe S, Pratt GA, Scaletta DB, Ha Y, Hill DE,
            Aigner S, Yeo GW
  TITLE     Large-scale tethered function assays identify factors that regulate 
            mRNA stability and translation.
  JOURNAL   Nat Struct Mol Biol. 2020 Oct;27(10):989-1000. doi: 
            10.1038/s41594-020-0477-6. Epub 2020 Aug 17.
  PUBMED    32807991
REFERENCE   2  (bases 1 to 10780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41594-020-0477-6"; journalName: "Nat Struct Mol Biol"; 
            date: "2020-10"; volume: "27"; issue: "10"; pages: "989-1000"
COMMENT     Sequence Label: SFRS2IP
FEATURES             Location/Qualifiers
     source          1..10780
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     1196..1215
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1196..1214
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1293..1317
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5737..5761)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5886..5927
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(6382..6399)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6388..6612
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6745..6764)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    6896..6943
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             6951..7973
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             6951..7973
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    8103..8224
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8140..8159)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8194..8213
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8273..8289)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8273..8289)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8286..8308)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8297..8313
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8321..8327,8328..8345,8346..8351))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8366..8387
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8504..8521)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8675..9263)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8755..8774)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9434..10225,10226..10294))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10057..10076
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10295..10399)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10474..10493)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gaattagctt ggtactaata
     1201 cgactcacta tagggagacc caagctggct aggtaagctt ggtaccgagc tcggatccac
     1261 tagtccagtg tggtggaatt ctgcagatat caacaagttt gtacaaaaaa gcagaagggc
     1321 cgtcaaggcc caccatgaag aagaaaactg tatgtaccct aaatatggga gataagaagt
     1381 atgaagacat ggaaggtgaa gaaaacggag ataatactat ttccactggt ctgttgtaca
     1441 gtgaggctga cagatgccca atatgtctta attgtctatt agaaaaggaa gttggttttc
     1501 cagaaagctg taatcatgtc ttctgtatga cttgtattct taaatgggca gagacactgg
     1561 cttcatgtcc tattgaccgt aaaccttttc aggcagtgtt taaattcagt gcattggaag
     1621 gttatgttaa ggttcaagta aaaaaacagc tgagagaaac aaaagacaag aaaaatgaaa
     1681 actcatttga gaaacaggtc tcctgtcatg aaaattctaa aagctgtata agaagaaaag
     1741 ccatcgtaag agaagatcta ttaagtgcaa aagtttgtga cttgaagtgg atacatagaa
     1801 actctttata cagtgaaaca ggaggaaaga aaaatgcagc aataaagata aataagcctc
     1861 agagatcaaa ttggagtaca aatcagtgct tcagaaattt tttctccaat atgttttctt
     1921 ctgttagcca ctctggagaa tcttccttta cctatagagc ttattgtaca gaatttatag
     1981 aagccagtga aatcagtgca ttgattaggc agaagagaca tgaactggaa ttgtcatggt
     2041 ttcctgatac attacctgga attggaagaa ttggttttat accctggaat gttgaaacag
     2101 aagtccttcc tctcatttct tctgtgttgc caagaactat ttttccaaca agtaccatat
     2161 ctttcgaaca ttttggtact tcttgcaagg gatatgcatt agcacatact caagaagggg
     2221 aagaaaagaa gcaaacttct ggtacatcaa ataccagagg atcaagacga aaacctgcaa
     2281 tgacaactcc tacaaggagg tctacacgta acacaagagc tgaaacagcc agtcagtctc
     2341 agagatcccc aatatcagac aattctgggt gtgatgcccc aggtaacagt aatccatctt
     2401 taagtgttcc ctcttcagct gagtcagaaa agcaaacaag acaggctcca aaacggaagt
     2461 ctgtaagaag aggaagaaaa ccacctttac tgaaaaagaa acttcggagc tctgtagctg
     2521 cccctgaaaa atcatcttcc aatgattcag tagatgaaga aacagcagaa tctgacacat
     2581 cacctgtgtt agaaaaagag caccaaccag atgtagacag tagtaacatt tgtactgtgc
     2641 agactcatgt agaaaaccag tctgctaatt gcttgaaaag ttgcaatgag caaatagaag
     2701 aaagtgagaa gcatactgca aattatgata cagaggaaag agtaggatct tcatcttctg
     2761 agtcttgtgc tcaagatctt cctgtgctag ttggtgagga aggggaagtt aaaaaactcg
     2821 agaatacagg tatagaggct aatgttttgt gtttggaaag tgagatttct gaaaatattc
     2881 ttgaaaaagg aggtgatcca ttggaaaagc aagaccagat atctggactt tcacaatcag
     2941 aggtaaagac agatgtatgt acagttcatc ttccaaatga ttttcctaca tgtttaacat
     3001 ctgaaagcaa agtgtaccaa cctgtatctt gtcccctaag tgacttatct gagaatgtag
     3061 agtcagtggt taatgaagaa aaaataacag agagttccct agtagaaatt actgaacata
     3121 aagattttac actaaaaaca gaggagctta tagagagccc caagttagaa tcttctgagg
     3181 gtgaaattat acagacagtg gacagacaat ctgttaagag cccagaggtt caattgcttg
     3241 ggcatgttga aactgaagat gtagaaataa ttgcaacatg tgatactttt gggaatgaag
     3301 atttcaataa tattcaagac tctgaaaata acttactaaa aaataatctt ctgaacacca
     3361 aattggaaaa atctttagaa gaaaagaatg aatcgctgac cgaacatcct agatctacag
     3421 agttgcctaa aacacacatt gaacagattc agaagcattt tagtgaggac aacaatgaaa
     3481 tgatacctat ggagtgtgat tcattttgca gtgaccaaaa tgaatctgaa gttgaaccat
     3541 ctgtaaatgc tgatcttaaa caaatgaatg aaaattctgt gacacactgt tctgaaaata
     3601 atatgccgtc ttctgatctt gcggatgaaa aggttgaaac tgtttctcaa ccatctgaaa
     3661 gcccaaaaga taccatagat aaaaccaaaa agcctcgtac tcgaagatct agatttcatt
     3721 ctccatctac aacttggtca cccaacaaag acactccaca agaaaagaag cggccccagt
     3781 ctccatctcc cagaagagaa actgggaaag aaagcaggaa gtctcaatca ccatctccta
     3841 agaatgagtc agccagaggc cggaaaaaat cccgttctca gtccccaaaa aaggatattg
     3901 caagagaaag gaggcaatct cagtctcggt ctccaaaaag ggatactact agggaaagca
     3961 gaagatctga atcactgtcc ccaagaagag aaacttctag agagaacaaa agatctcagc
     4021 caagagtgaa agattcttcc ccaggagaaa aatccaggtc ccagagcaga gaacgagaaa
     4081 gtgatagaga tgggcagagg agagagagag aaaggagaac cagaaagtgg tctaggtcca
     4141 gatctcattc taggtccccc tcaagatgta gaacaaaaag taagagttca tcatttggta
     4201 gaattgacag agatagttac tctccccggt ggaagggaag atgggcaaat gatggttgga
     4261 gatgtccacg aggaaatgat cggtacagaa agaatgaccc agagaaacag aatgaaaata
     4321 caagaaaaga aaaaaatgac atccatctag atgctgatga tccaaattct gctgacaaac
     4381 atagaaatga ctgtcccaat tggataacag aaaaaataaa ctctgggcct gatccaagaa
     4441 ccagaaatcc agaaaagttg aaagagtctc attgggaaga aaatagaaat gaaaattcag
     4501 gaaattcttg gaataaaaac tttggttctg gttgggtatc taaccgtggt agaggcagag
     4561 gcaaccgtgg cagaggcact tacagaagta gttttgccta taaagatcag aatgaaaatc
     4621 ggtggcaaaa tcgaaaaccc ctctcaggga attcaaacag ttcagggagt gaatctttca
     4681 agtttgtgga acagcaatcc tataagcgaa aaagtgaaca ggagttctca tttgatacac
     4741 cagcagatag atctggatgg acatctgcat ccagctgggc cgtgagaaag actttgccag
     4801 cagatgtaca aaactactac tcacgacgag gcagaaattc ttcaggtcca cagtctggat
     4861 ggatgaaaca agaggaggaa acatctggac aggattctag cctaaaagac caaacaaacc
     4921 agcaagttga tggttctcag ctacctataa atatgatgca accgcaaatg aatgtaatgc
     4981 agcaacaaat gaatgcacaa caccagccta tgaatatctt cccatatcca gtgggtgttc
     5041 atgctccttt gatgaacatc caacgcaatc catttaacat tcatcctcag ctacccttgc
     5101 atctccacac aggagtgccc ctcatgcagg tagccactcc taccagtgta tctcagggac
     5161 taccaccacc accaccccct cccccaccat cccaacaagt caactacatt gcttcacaac
     5221 cagatggaaa gcaattgcag ggtattccta gttcttctca tgtaagtaat aacatgagta
     5281 caccagtttt gcctgctccg acagcagccc caggaaatac gggaatggtt cagggaccaa
     5341 gttctggtaa tacttcgtca tcaagtcaca gcaaagcctc taatgctgct gtaaaattgg
     5401 cagaaagcaa agtaagtgtt gcagtggaag ccagcgcaga tagctcgaag acagacaaga
     5461 aattgcaaat tcaagaaaaa gcagcacaag aggtaaaatt ggccatcaag ccattttacc
     5521 aaaataaaga tatcaccaag gaagaatata aagaaattgt acggaaagca gtagataaag
     5581 tttgtcatag taagagtgga gaagtaaatt ctactaaagt ggcaaatctg gttaaagcct
     5641 atgtagacaa atacaaatat tcacggaagg ggagccaaaa gaaaactctg gaagaacctg
     5701 tgtctactga aaaaaacata ggctcaggcc tcatgggccc agctttcttg tacaaagtgg
     5761 ttgatatcca gcacagtggc ggccgctcga gtctagaggg cccgcggttc gaaaccagcg
     5821 gaggcggcgg aagcggggga ggaggcagcg gcggaggcgg cagcgggggc ggcggaagcg
     5881 ctaccggtaa gcctatccct aaccctctcc tcggtctcga ttctacgcgt accggtatgg
     5941 cttctaactt tactcagttc gttctcgtcg acaatggcgg aactggcgac gtgactgtcg
     6001 ccccaagcaa cttcgctaac ggggtcgctg aatggatcag ctctaactcg cgatcacagg
     6061 cttacaaagt aacctgtagc gttcgtcaga gctctgcgca gaatcgcaaa tacaccatca
     6121 aagtcgaggt gcctaaagtg gcaacccaga ctgttggtgg tgaagagctt cctgtagccg
     6181 gatggagatc ttacttaaat atggaactaa ccattccaat tttcgccacg aattccgact
     6241 gcgagcttat tgttaaggca atgcaaggtc tcctaaaaga tggaaacccg attccctcgg
     6301 ccatcgcggc caactccggc atctacggag gtggaggtgg atctcgtacg ggttagtaat
     6361 gagtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt
     6421 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
     6481 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg
     6541 tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg
     6601 tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg
     6661 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     6721 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     6781 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     6841 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt acctagaagt
     6901 tcctattccg aagttcctat tctctagaaa gtataggaac ttccttggcc aaaaagcctg
     6961 aactcaccgc gacgtctgtc gagaagtttc tgatcgaaaa gttcgacagc gtctccgacc
     7021 tgatgcagct ctcggagggc gaagaatctc gtgctttcag cttcgatgta ggagggcgtg
     7081 gatatgtcct gcgggtaaat agctgcgccg atggtttcta caaagatcgt tatgtttatc
     7141 ggcactttgc atcggccgcg ctcccgattc cggaagtgct tgacattggg gaattcagcg
     7201 agagcctgac ctattgcatc tcccgccgtg cacagggtgt cacgttgcaa gacctgcctg
     7261 aaaccgaact gcccgctgtt ctgcagccgg tcgcggaggc catggatgcg atcgctgcgg
     7321 ccgatcttag ccagacgagc gggttcggcc cattcggacc gcaaggaatc ggtcaataca
     7381 ctacatggcg tgatttcata tgcgcgattg ctgatcccca tgtgtatcac tggcaaactg
     7441 tgatggacga caccgtcagt gcgtccgtcg cgcaggctct cgatgagctg atgctttggg
     7501 ccgaggactg ccccgaagtc cggcacctcg tgcacgcgga tttcggctcc aacaatgtcc
     7561 tgacggacaa tggccgcata acagcggtca ttgactggag cgaggcgatg ttcggggatt
     7621 cccaatacga ggtcgccaac atcttcttct ggaggccgtg gttggcttgt atggagcagc
     7681 agacgcgcta cttcgagcgg aggcatccgg agcttgcagg atcgccgcgg ctccgggcgt
     7741 atatgctccg cattggtctt gaccaactct atcagagctt ggttgacggc aatttcgatg
     7801 atgcagcttg ggcgcagggt cgatgcgacg caatcgtccg atccggagcc gggactgtcg
     7861 ggcgtacaca aatcgcccgc agaagcgcgg ccgtctggac cgatggctgt gtagaagtac
     7921 tcgccgatag tggaaaccga cgccccagca ctcgtccgag ggcaaaggaa tagcacgtac
     7981 tacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc
     8041 gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc
     8101 ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     8161 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     8221 cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc
     8281 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     8341 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     8401 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     8461 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     8521 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     8581 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     8641 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     8701 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     8761 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     8821 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     8881 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     8941 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     9001 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     9061 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     9121 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     9181 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     9241 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     9301 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     9361 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     9421 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     9481 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     9541 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     9601 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     9661 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     9721 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     9781 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     9841 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     9901 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     9961 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
    10021 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
    10081 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
    10141 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
    10201 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
    10261 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
    10321 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
    10381 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg
    10441 gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
    10501 aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat
    10561 ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc ttagggttag
    10621 gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt gattattgac
    10681 taggcttttg caaaaagctt tgcaaagatg gataaagttt taaacagaga ggaatctttg
    10741 cagctaatgg accttctagg tcttgaaagg agtgggaatt
//
