LOCUS       sequence-376023-       10108 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For use in RBP tethering screen.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10108)
  AUTHORS   Luo EC, Nathanson JL, Tan FE, Schwartz JL, Schmok JC, Shankar A, 
            Markmiller S, Yee BA, Sathe S, Pratt GA, Scaletta DB, Ha Y, Hill DE,
            Aigner S, Yeo GW
  TITLE     Large-scale tethered function assays identify factors that regulate 
            mRNA stability and translation.
  JOURNAL   Nat Struct Mol Biol. 2020 Oct;27(10):989-1000. doi: 
            10.1038/s41594-020-0477-6. Epub 2020 Aug 17.
  PUBMED    32807991
REFERENCE   2  (bases 1 to 10108)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41594-020-0477-6"; journalName: "Nat Struct Mol Biol"; 
            date: "2020-10"; volume: "27"; issue: "10"; pages: "989-1000"
COMMENT     Sequence Label: SKIV2L
FEATURES             Location/Qualifiers
     source          1..10108
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     1196..1215
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1196..1214
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1293..1317
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5065..5089)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5214..5255
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(5710..5727)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5716..5940
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6073..6092)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    6224..6271
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             6279..7301
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             6279..7301
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    7431..7552
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7468..7487)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7522..7541
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7601..7617)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7601..7617)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7614..7636)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7625..7641
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7649..7655,7656..7673,7674..7679))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7694..7715
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7832..7849)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8003..8591)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8083..8102)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8762..9553,9554..9622))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9385..9404
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9623..9727)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9802..9821)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gaattagctt ggtactaata
     1201 cgactcacta tagggagacc caagctggct aggtaagctt ggtaccgagc tcggatccac
     1261 tagtccagtg tggtggaatt ctgcagatat caacaagttt gtacaaaaaa gcaggctcca
     1321 ccatgatgga tacagagcga cttgtgctac cccctccaga tcccctggac ctaccccttc
     1381 gggccgtgga gctcggatgc acggggcact gggagctgct gaacttgcct ggagctccag
     1441 agagtagcct tccccatggc ctccctcctt gtgccccaga tctgcagcaa gaagcagaac
     1501 agttgtttct gtcatcccca gcctggctgc ctctgcatgg tgtggagcac tcagcccgaa
     1561 aatggcagag gaagacggat ccctggtctc ttttggctgt gctgggagcc ccagtcccat
     1621 ccgacctaca ggcccaaaga cacccaacca caggccagat actgggttac aaagaggtct
     1681 tgctggagaa cacaaatctc tcggctacaa cctccttgtc tcttcgccgg cctccagggc
     1741 cagcctccca gtccttatgg ggaaatccaa ctcggtatcc cttctggcca ggggggatgg
     1801 atgaacccac cataacagat ctgaacacac gggaggaggc tgaggaggag atagactttg
     1861 agaaagatct tcttactatt ccacctggtt tcaagaaagg catggacttt gcaccaaaag
     1921 attgtccaac tccagctcct ggactactaa gccttagctg tctgttggag cctctggatt
     1981 tgggtggggg tgacgaggat gagaatgagg cagtgggaca gccaggaggt cccagagggg
     2041 acactgtttc agcctctccc tgcagtgctc ccctggcccg agcaagcagc ttggaagacc
     2101 tagtgttgaa ggaagcgtcc acagctgtat ccaccccaga ggccccagag cctccatctc
     2161 aggagcagtg ggccatccct gtggacgcca cctcccctgt tggtgatttc tatcgcctca
     2221 ttccccagcc agccttccag tgggcatttg agccagatgt gtttcagaaa caggccatcc
     2281 tgcacttgga acggcatgac tctgtctttg tcgcagctca cacatctgca ggaaaaacag
     2341 ttgtggctga atatgccatt gccctggccc agaaacacat gacacgcacc atctacactt
     2401 cgcccatcaa ggccctgagc aaccagaagt tccgggactt ccgaaacaca ttcggggatg
     2461 tggggctgct caccggggat gtacagctgc atccggaggc ctcctgcctc atcatgacca
     2521 cagagatcct tcgctccatg ctgtacagtg gctcagatgt tattcgggac ctggagtggg
     2581 tcatctttga tgaggttcac tatatcaacg atgtcgagcg tggggtcgtg tgggaggagg
     2641 tgcttatcat gctacctgac cacgtttcta tcatccttct gagtgccacc gtccccaacg
     2701 cccttgagtt tgctgactgg attgggcggc tgaagcgtcg tcagatctat gtgattagca
     2761 ctgtaacccg ccccgtgccc ctggagcact atcttttcac agggaacagc tccaagaccc
     2821 agggggagct ctttttgttg ctggactccc gaggagcctt ccatacaaaa gggtactatg
     2881 cagctgtgga ggccaagaag gagagaatga gcaaacacgc ccagaccttt ggggccaagc
     2941 agcccacaca tcaggggggc cctgcacagg accgcggagt gtacctgtcc ctcctggcct
     3001 ccctccgcac acgtgcccag ttgcccgtgg tggtgttcac cttctcccgg ggccgctgtg
     3061 atgagcaggc ctcaggcctc acctcccttg acctcaccac cagttcggag aagagcgaga
     3121 tccacctctt cctgcagcgc tgccttgctc gcctccgtgg ctctgaccgc cagctgcccc
     3181 aggtcctgca catgtcagag ctcctgaatc gcggcctggg tgtgcaccat agcggcatcc
     3241 tgcccatcct caaggagatc gtggagatgc tcttcagccg tggcctggtc aaggtcttgt
     3301 ttgccacaga gacctttgcc atgggagtaa acatgcctgc tcgtacagta gtgtttgact
     3361 ccatgcgcaa acacgatggc tccaccttcc gggacctgct ccctggggag tatgtgcaga
     3421 tggcaggccg ggcagggcgg aggggcctgg accccacagg caccgttatc ctgctctgca
     3481 agggccgagt gcccgagatg gcagacctgc accgcatgat gatggggaag ccgtcccagc
     3541 tgcagtccca gttccgcctc acgtacacta tgatcctcaa cttgctgcga gtggatgccc
     3601 tcagggtgga ggacatgatg aagaggagct tctctgagtt tccctcccgc aaagacagca
     3661 aggcccatga acaggccctg gctgaactga ccaagaggct gggagctttg gaggagcctg
     3721 acatgactgg ccaactggtc gacctgcctg aatattacag ctggggggag gaactgacag
     3781 agacccagca catgatccag cgacgcatca tggagtctgt gaacgggctg aagtctctct
     3841 cagcaggaag ggtggtggtt gtgaagaatc aggagcatca caacgcattg ggagtgatcc
     3901 tacaggtctc ctcgaactcc accagcagag tattcacaac cctggtcttg tgtgataagc
     3961 ccttgtccca ggacccacag gacagggggc cagccactgc agaggtgccc tatccagatg
     4021 acctcgtggg attcaagctg ttcctgcctg aagggccttg tgaccacacc gtggtcaagc
     4081 tccagccagg agatatggct gccatcacca ccaaggtgct ccgggtgaat ggggagaaga
     4141 tcttggagga cttcagcaag aggcagcagc caaaattcaa gaaggatcct ccccttgcag
     4201 ccgtgaccac tgctgtccag gaactgctgc gtctggctca ggcccaccca gccggacctc
     4261 ccaccctcga ccctgtcaat gacctgcagc tcaaagatat gtcagttgta gagggtgggc
     4321 tccgggcccg gaagctggag gagctgatcc agggggctca gtgtgtacac agcccccgtt
     4381 ttcctgccca gtacctgaag ctgcgggagc gaatgcagat acagaaggag atggagcggc
     4441 tgcgcttcct actgtcggat cagtcattgc tgctgcttcc tgagtaccat cagcgagtag
     4501 aggtgctccg aaccctgggt tacgtggacg aggtgggcac tgtgaagctg gcagggcggg
     4561 tggcttgtgc catgagcagc catgagttgc tcctcactga gctcatgttt gacaatgcac
     4621 tgagcaccct gcggcctgag gagattgctg ccttgctctc tggcctggtc tgccagagcc
     4681 ctggggacgc tggggatcag ctcccaaaca ccctcaagca gggaatagaa cgtgtccggg
     4741 ctgtggccaa gcggattggt gaggtccagg tggcttgtgg cctgaaccag acggtggagg
     4801 aatttgtggg ggagctgaat tttgggctgg ttgaggttgt atatgagtgg gcccggggca
     4861 tgcccttctc cgagttggca gggctctcag ggacccctga gggcctggtg gtccgctgca
     4921 ttcagcgcct ggctgagatg tgtcgctcac tgcggggggc agcccgcctg gtaggagagc
     4981 ctgtgctggg tgccaagatg gagacagcgg ctaccttgct acggcgggac atcgtatttg
     5041 cggccagcct ctacacccag ttggacccag ctttcttgta caaagtggtt gatatccagc
     5101 acagtggcgg ccgctcgagt ctagagggcc cgcggttcga aaccagcgga ggcggcggaa
     5161 gcgggggagg aggcagcggc ggaggcggca gcgggggcgg cggaagcgct accggtaagc
     5221 ctatccctaa ccctctcctc ggtctcgatt ctacgcgtac cggtatggct tctaacttta
     5281 ctcagttcgt tctcgtcgac aatggcggaa ctggcgacgt gactgtcgcc ccaagcaact
     5341 tcgctaacgg ggtcgctgaa tggatcagct ctaactcgcg atcacaggct tacaaagtaa
     5401 cctgtagcgt tcgtcagagc tctgcgcaga atcgcaaata caccatcaaa gtcgaggtgc
     5461 ctaaagtggc aacccagact gttggtggtg aagagcttcc tgtagccgga tggagatctt
     5521 acttaaatat ggaactaacc attccaattt tcgccacgaa ttccgactgc gagcttattg
     5581 ttaaggcaat gcaaggtctc ctaaaagatg gaaacccgat tccctcggcc atcgcggcca
     5641 actccggcat ctacggaggt ggaggtggat ctcgtacggg ttagtaatga gtttaaaccc
     5701 gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg
     5761 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa
     5821 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca
     5881 gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg
     5941 cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg
     6001 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     6061 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     6121 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     6181 tcgaccccaa aaaacttgat tagggtgatg gttcacgtac ctagaagttc ctattccgaa
     6241 gttcctattc tctagaaagt ataggaactt ccttggccaa aaagcctgaa ctcaccgcga
     6301 cgtctgtcga gaagtttctg atcgaaaagt tcgacagcgt ctccgacctg atgcagctct
     6361 cggagggcga agaatctcgt gctttcagct tcgatgtagg agggcgtgga tatgtcctgc
     6421 gggtaaatag ctgcgccgat ggtttctaca aagatcgtta tgtttatcgg cactttgcat
     6481 cggccgcgct cccgattccg gaagtgcttg acattgggga attcagcgag agcctgacct
     6541 attgcatctc ccgccgtgca cagggtgtca cgttgcaaga cctgcctgaa accgaactgc
     6601 ccgctgttct gcagccggtc gcggaggcca tggatgcgat cgctgcggcc gatcttagcc
     6661 agacgagcgg gttcggccca ttcggaccgc aaggaatcgg tcaatacact acatggcgtg
     6721 atttcatatg cgcgattgct gatccccatg tgtatcactg gcaaactgtg atggacgaca
     6781 ccgtcagtgc gtccgtcgcg caggctctcg atgagctgat gctttgggcc gaggactgcc
     6841 ccgaagtccg gcacctcgtg cacgcggatt tcggctccaa caatgtcctg acggacaatg
     6901 gccgcataac agcggtcatt gactggagcg aggcgatgtt cggggattcc caatacgagg
     6961 tcgccaacat cttcttctgg aggccgtggt tggcttgtat ggagcagcag acgcgctact
     7021 tcgagcggag gcatccggag cttgcaggat cgccgcggct ccgggcgtat atgctccgca
     7081 ttggtcttga ccaactctat cagagcttgg ttgacggcaa tttcgatgat gcagcttggg
     7141 cgcagggtcg atgcgacgca atcgtccgat ccggagccgg gactgtcggg cgtacacaaa
     7201 tcgcccgcag aagcgcggcc gtctggaccg atggctgtgt agaagtactc gccgatagtg
     7261 gaaaccgacg ccccagcact cgtccgaggg caaaggaata gcacgtacta cgagatttcg
     7321 attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct
     7381 ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta
     7441 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     7501 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     7561 gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt
     7621 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     7681 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     7741 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     7801 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     7861 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     7921 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     7981 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     8041 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     8101 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     8161 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     8221 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     8281 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     8341 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     8401 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     8461 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     8521 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     8581 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     8641 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     8701 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     8761 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     8821 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     8881 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     8941 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     9001 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     9061 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     9121 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     9181 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     9241 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     9301 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     9361 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     9421 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     9481 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     9541 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     9601 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     9661 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     9721 ttccgcgcac atttccccga aaagtgccac ctgacgtcga cggatcggga gatctcccga
     9781 tcccctatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatct
     9841 gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt aagctacaac
     9901 aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt agggttaggc gttttgcgct
     9961 gcttcgcgat gtacgggcca gatatacgcg ttgacattga ttattgacta ggcttttgca
    10021 aaaagctttg caaagatgga taaagtttta aacagagagg aatctttgca gctaatggac
    10081 cttctaggtc ttgaaaggag tgggaatt
//
