LOCUS       sequence-376033-        9212 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mec-3 fluorescent neural reporter driving nuclear CyOFP1 and 
            mTagBFP2 expression (refer to NeuroPAL paper for expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9212)
  AUTHORS   Yemini E, Lin A, Nejatbakhsh A, Varol E, Sun R, Mena GE, Samuel ADT,
            Paninski L, Venkatachalam V, Hobert O
  TITLE     NeuroPAL: A Multicolor Atlas for Whole-Brain Neuronal Identification
            in C. elegans.
  JOURNAL   Cell. 2021 Jan 7;184(1):272-288.e11. doi: 
            10.1016/j.cell.2020.12.012. Epub 2020 Dec 29.
  PUBMED    33378642
REFERENCE   2  (bases 1 to 9212)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.012"; journalName: "Cell"; date: "2021-01-7-
            7"; volume: "184"; issue: "1"; pages: "272-288.e11"
COMMENT     Sequence Label: pEY51
FEATURES             Location/Qualifiers
     source          1..9212
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6..22
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3200..3220
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3242..3262
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4514..4567
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4577..4597
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4619..4639
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(6803..6825)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6925..6944
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7022..7040)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7108..7212
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7213..7281,7282..8073)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7431..7450)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8244..8832
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8733..8752
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8986..9003
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9120..9141
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9156..9161,9162..9179,9180..9186)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9194..9210
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(9199..9212,1..9)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcctgcagg aatgaacaaa
      181 ttgtgaaatt catttttgat tgaatctact aaaattaaag tcgttgcatg tatctttata
      241 cagtttctat tatctgaagt ttgtagtccg tctgataatg ggacttttat ttcacttttt
      301 accccacatt ttgctttttt ttttagacag atactactga taataattct cgccccataa
      361 ccaacattag tttcattgtt cttcaagaac caataaaata gcagctgtta tgtaataatg
      421 tattcacaga caactgatgg ctagtgttgt atgtgcacgt caactgccat gtaggtactg
      481 aacatataat ggtgtcgagg tcgtcaatct gaatttccaa tgagaatgta ttcgagaacg
      541 atgcaacaaa ttggcccgtt tatggtggtg tcagattttt aattttattt tcttcatttt
      601 ttcatgtgtt ttatgtctat tatcaaacag tagctttttg gctcttatca gtcaagcaaa
      661 aatataatta ttttttgtta aaccgtagat tgtagttatc ttatttgaaa tcacatggtt
      721 ttttgtgtca gtaggaatag ttttcaaatt gttttgtgac aacgaggaat gaaaaaagaa
      781 gaaattacat aaataagatt taaatctcaa tctaaaaaca tttttagaac tttttcaaag
      841 atatgttgaa ttctggcaac tttaggtagt tttaaatttt ccacgaaatt tcaaatcaaa
      901 tgttaaccag ttggtaatat ttgatctaaa gttcatacta atctgtaatt ttttggtgta
      961 atgcaaaata ttagaatttt aaaattttga aatcaattgt tagaaagcgt ctccctgttt
     1021 tgcatatttt tgtcgtttat tttgttgttt gtagatttgg gtagatcttc agtttatatg
     1081 atcttcgatt gtgctatttt cggattttta tggataaaaa ttacatctta aaactctcgg
     1141 aataaactat caaaatgagg agcgatgtgt ttttttttaa ttttgccctg caggtacccg
     1201 gagtagttgg cgaagataga aatggtgtta tcttatataa attcagactt tctctgttaa
     1261 aattttaacc accaaaaaat atagaattta taaaaagttt cattttgaaa ttttatcttt
     1321 tggggccatt tcactacctt atcaggaaga aatgtgtttc atcagaaatt gaatttttca
     1381 tcttttcaga aagtgtacct aataccgtat catgctcata ttcacgtgaa taaggcttct
     1441 gcccagttct gtgattcaag gaaaactacg aaaaatgatc tcattttctt tgttttatca
     1501 ccgagctggt gtgtcatcac acacagtctg tttcttgata aaaacgcgca gaagttagag
     1561 cattcattgt gattttgggt ctttaatttc tgtaaaacaa ataaagtgac aagtttatca
     1621 tctgcctatt ttatcagtta tatgcatggt tagttgcaaa taagggagta gaaattgact
     1681 aaaactcaaa atgagcactg aaacgagggg ttttgttagt tgcgaaataa caattaatac
     1741 aatctggttg attactttgt aaaaggataa taaaatatgc aaaacttggc gtattctgag
     1801 atttcattcc taaagacatt tatcaaaaca aatgttttct gcaattttct aaattcttgg
     1861 aattctgccc agcagcctct aatatcgcgt tttacttaga aaatgacttt tggtttcatt
     1921 ttctcatttt ctggcttcta tttttcacaa atgcaaatat ttacatttaa attagtactt
     1981 tcatttctac aaagatcaaa tttaacatta ttgacaggtt tactgttatc ttgtgacagt
     2041 tccgtactgt caatcaaaac gaaaattaaa actcatgaaa tatcaatgaa cataaattga
     2101 aaaaaaaacc cagttgaaag gagaaaataa gcgaaacaca ccaacaactg agtagttacg
     2161 ctcacaattt ggaaatgatt ttgaaaactc tctggactgc atttcaattt ttaacaaaca
     2221 atgaatacat actgatagtt tttgtacttc tcggcattca gaggcaactt cctgccaaca
     2281 acctggcaac ttatttagtg ccactaaaat aagttgccaa attgttggca ggaagttgcc
     2341 tccaaatgcc gagttggagc aaatgattcg atatgatttt aaggatacct acgcatgcct
     2401 cctgccaaga cacgcaccca cctactgaaa tgtcgcggaa ttttacctcc caaactatag
     2461 attgggtgaa acttcactgt aaaagctttt tttaagtagt aggctggcag taatgaagac
     2521 gtccatacca taatttttaa tgttgtatag aaaatctgaa ttctcaagta taacattttc
     2581 caataattag catagtcaga aactctccac cgccgttccg ttcctttttt ttgtaactat
     2641 ggagtaatag aacaagatta ttcaaattca aatttttata aaactttaca acgtcctttc
     2701 catatattct tcacacaaca tttgaaaaaa caacaaattc attcgaaatg cattgcccat
     2761 aatgaatcga ccgaaaaaca caagtgaccg tcaggagatc gatagagaga gcccgttcca
     2821 actagacaac tttttagtgc ttatccttac acacactttc tagcttcata agaaatgcat
     2881 ctattatcgt caccatttgg agacaccagt tttagcgcac attaataatc gatcagggtc
     2941 tagagactcc tgttggattg gcatgccacg cctacatgac atctggctct tgacgggtga
     3001 tttgttacag tgagtagcca caatttcgcg cattttcttc taaatttcac tgttatttgt
     3061 tcttctgatt tatttcattt gaactcccgg gattggccaa aggacccaaa ggtatgtttc
     3121 gaatgatact aacataacat agaacatttt caggaggacc cttgcttgga gggtaccgag
     3181 ctcagaaaaa atgaccgctc caaagaagaa gcgcaaggtt ccagtcgaga agatgactgc
     3241 cccaaaaaag aaacgtaagg tcccgcgggt aagtttaaac agttcggtac taactaacca
     3301 tacatattta aattttcagc gcgctgtgtc aaaaggagaa gagcttatta aggagaatat
     3361 gcgttccaag ctctacctcg agggatccgt caacggacac caattcaagt gcacccacga
     3421 gggagaggga aagccatacg agggaaagca aaccaaccgt atcaaggtcg tcgagggagg
     3481 accactccca ttcgccttcg acatcctcgc cacccacttc atgtacggat ccaaggtaag
     3541 tttaaacata tatatactaa ctaaccctga ttatttaaat tttcaggtct tcatcaagta
     3601 cccagccgac ctcccagact acttcaagca atccttccca gagggattca cctgggagcg
     3661 tgtcatggtc ttcgaggacg gaggagtcct caccgccacc caagacacct ccctccaaga
     3721 cggagagctc atctacaacg tcaaggtccg tggagtcaac ttcccagcca acggaccagt
     3781 catgcaaaag aagaccctcg gatgggagcc atccaccgag accatgtacc cagccgacgg
     3841 aggactcgag ggacgttgcg acaaggtaag tttaaacatg attttactaa ctaactaatc
     3901 tgatttaaat tttcaggccc tcaagctcgt cggaggagga cacctccacg tcaacttcaa
     3961 gaccacctac aagtccaaga agccagtcaa gatgccagga gtccactacg tcgaccgtcg
     4021 tctcgagcgt atcaaggagg ccgacaacga gacctacgtc gagcaatacg agcacgccgt
     4081 cgcccgttac tccaacctcg gaggaggaat ggacgagctc tacaagccta ggataccggt
     4141 gggtgaagac ccaccaaagc catctgccaa gggagccaag aaggccgcca agaccgttac
     4201 gaagccaaag gacggaaaga agagacgtca tgcccgtaag gaatcatact ccgtctacat
     4261 ctaccgtgtc ctcaagcaag ttcatccaga cactggagtt tcctccaaag ccatgtctat
     4321 catgaactct tttgtcaacg atgtcttcga gcgtattgct gctgaagcat cccgtcttgc
     4381 tcactacaac aagcgttcca caatctcatc ccgcgaaatt cagaccgctg tccgtctgat
     4441 ccttccagga gagcttgcca agcacgccgt gtctgaggga accaaggccg ttaccaagta
     4501 cacttccagc aaggagggac gtggatccct cctcacctgc ggagacgtcg aggagaaccc
     4561 aggaccaatg accgctccaa agaagaagcg caaggttcca gtcgagaaga tgactgcccc
     4621 aaaaaagaaa cgtaaggtcc cgcggatgtc agagcttatt aaggagaata tgcatatgaa
     4681 actgtacatg gaaggaaccg tggacaacca tcactttaag tgcacttcgg agggggaagg
     4741 caaaccgtac gagggcaccc agaccatgcg aatcaaggtg gtcgagggag gcccactgcc
     4801 cttcgccttc gacattctgg taagtttaaa catatatata ctaactaacc ctgattattt
     4861 aaattttcag gctactagct tcctgtacgg cagcaagact tttattaatc acacccaggg
     4921 catcccggac ttcttcaaac agtcgttccc tgagggcttc acatgggagc gtgtgaccac
     4981 atacgaagac ggtggcgttc tcaccgctac ccaggacacc agcctccagg acggttgcct
     5041 catatataat gtggtaagtt taaacagttc ggtactaact aaccatacat atttaaattt
     5101 tcagaagatc cggggagtaa acttcacatc caatggtccg gttatgcaga agaaaacact
     5161 cggctgggag gccttcaccg agacgctgta tccggctgac ggaggcctgg aaggtcgaaa
     5221 cgacatggcc ctgaagctcg tcggcggtag ccatctcatc gcaaacgcta agaccacata
     5281 tagatccaag aaaccggtaa gtttaaacat gattttacta actaactaat ctgatttaaa
     5341 ttttcaggct aagaacctta aaatgccggg agtctactat gttgactaca gactggaacg
     5401 gatcaaggag gccaacaacg agacctacgt cgagcagcac gaggttgcag ttgccagata
     5461 ctgcgacctc ccgagcaaac tgggtcacaa gcttaatcct aggataccgg tgggtgaaga
     5521 cccaccaaag ccatctgcca agggagccaa gaaggccgcc aagaccgtta cgaagccaaa
     5581 ggacggaaag aagagacgtc atgcccgtaa ggaatcatac tccgtctaca tctaccgtgt
     5641 cctcaagcaa gttcatccag acactggagt ttcctccaaa gccatgtcta tcatgaactc
     5701 ttttgtcaac gatgtcttcg agcgtattgc tgctgaagca tcccgtcttg ctcactacaa
     5761 caagcgttcc acaatctcat cccgcgaaat tcagaccgct gtccgtctga tccttccagg
     5821 agagcttgcc aagcacgccg tgtctgaggg aaccaaggcc gttaccaagt acacttccag
     5881 caagtaggaa ttccaactga gcgccggtcg ctaccattac caacttgtct ggtgtcaaaa
     5941 ataatagggg ccgctgtcat cagagtaagt ttaaactgag ttctactaac taacgagtaa
     6001 tatttaaatt ttcagcatct cgcgcccgtg cctctgactt ctaagtccaa ttactcttca
     6061 acatccctac atgctctttc tccctgtgct cccaccccct atttttgtta ttatcaaaaa
     6121 acttctctta atttctttgt tttttagctt cttttaagtc acctctaaca atgaaattgt
     6181 gtagattcaa aaatagaatt aattcgtaat aaaaagtcga aaaaaattgt gctccctccc
     6241 cccattaata ataattctat cccaaaatct acacaatgtt ctgtgtacac ttcttatgtt
     6301 ttttacttct gataaatttt tttgaaacat catagaaaaa accgcacaca aaatacctta
     6361 tcatatgtta cgtttcagtt tatgaccgca atttttattt cttcgcacgt ctgggcctct
     6421 catgacgtca aatcatgctc atcgtgaaaa agttttggag tatttttgga atttttcaat
     6481 caagtgaaag tttatgaaat taattttcct gcttttgctt tttggggttt cccctattgt
     6541 ttgtcaagat ttcgaggacg gcgtttttct tgctaaaatc acaagtattg atgagcacga
     6601 tgcaagaaag atcggaagaa ggtttgggtt tgaggctcag tggaaggtga gtagaagttg
     6661 ataatttgaa agtggagtag tgtctatggg gtttttgcct taaatgacag aatacattcc
     6721 caatatacca aacataactg tttcctacta gtcggccgta cgggcccttt cgtctcgcgc
     6781 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
     6841 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
     6901 ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata
     6961 tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cggccttaag
     7021 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
     7081 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     7141 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     7201 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     7261 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     7321 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     7381 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     7441 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     7501 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     7561 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     7621 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     7681 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     7741 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     7801 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     7861 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     7921 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     7981 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     8041 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     8101 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     8161 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     8221 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     8281 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     8341 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     8401 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     8461 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     8521 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     8581 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     8641 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     8701 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     8761 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     8821 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     8881 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     8941 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     9001 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     9061 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
     9121 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
     9181 atgttgtgtg gaattgtgag cggataacaa tt
//
