LOCUS       sequence-376035-        5798 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  sri-1 fluorescent neural reporter driving nuclear CYOFP expression 
            (refer to NeuroPAL paper for expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5798)
  AUTHORS   Yemini E, Lin A, Nejatbakhsh A, Varol E, Sun R, Mena GE, Samuel ADT,
            Paninski L, Venkatachalam V, Hobert O
  TITLE     NeuroPAL: A Multicolor Atlas for Whole-Brain Neuronal Identification
            in C. elegans.
  JOURNAL   Cell. 2021 Jan 7;184(1):272-288.e11. doi: 
            10.1016/j.cell.2020.12.012. Epub 2020 Dec 29.
  PUBMED    33378642
REFERENCE   2  (bases 1 to 5798)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.012"; journalName: "Cell"; date: "2021-01-7-
            7"; volume: "184"; issue: "1"; pages: "272-288.e11"
COMMENT     Sequence Label: pEY36
FEATURES             Location/Qualifiers
     source          1..5798
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6..22
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1157..1177
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1199..1219
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(3389..3411)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3511..3530
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(3608..3626)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3694..3798
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3799..3867,3868..4659)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4017..4036)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4830..5418
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5319..5338
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5572..5589
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5706..5727
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5742..5747,5748..5765,5766..5772)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5780..5796
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(5785..5798,1..9)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcctgcaga agagaagcac
      181 aagcccggaa agtttaactt caagcccttg aaaattgcat tatattaatg ttgttcaagt
      241 tttagtaata aaaaattaga attggcagtt ttcaaaacat attagtttaa aggtggaatc
      301 gatttcagat tttgcttgaa atcgattgaa atggatcgtc accaagtata caaaactgta
      361 aaaaaattaa aaaccttttc taaattttat aattagttta aaaaatttga aatttaaaat
      421 tatttaattt tgcaaaataa aaacgatacg aattaaaatt caatttccct cgccaatgaa
      481 atactgcttt gacatttttg cgttttcagg caaaattcat cacttatttt cttccatatt
      541 taatttgatt gtcaattttt gtcagctttt ttctcctatc cccttcagta atgaagctat
      601 ttattatgtt ctaaataaac cttttcagca tttttcttca tttccttatt atgaatatct
      661 ttgcacacac tattggacaa atttaacatg ctatacttca atctttgttt ttgtcaatgg
      721 ttagtggata ttttaagaat tataagaagt caaacaagtt tttttggaaa actcgccgca
      781 atataaatca catattcaat tccctctttg cattttaagt atccttttct gaaaaatgtg
      841 tcagcaaatt gaacgttatt taaataaaaa caagaccgtc ttcgtagatg tctatggcaa
      901 aaactcatta aaactcaatg cattgttatt attttccttc ctctttcatt acgaattttt
      961 ttctttttca gcattgttca tttcagcttc actgcacaca gaaatcgtca tactctattc
     1021 agcgagttta gcatactaaa aacccgggat tggccaaagg acccaaaggt atgtttcgaa
     1081 tgatactaac ataacataga acattttcag gaggaccctt gcttggaggg taccgagctc
     1141 agaaaaaatg accgctccaa agaagaagcg caaggttcca gtcgagaaga tgactgcccc
     1201 aaaaaagaaa cgtaaggtcc cgcgggtaag tttaaacagt tcggtactaa ctaaccatac
     1261 atatttaaat tttcagcgcg ctgtgtcaaa aggagaagag cttattaagg agaatatgcg
     1321 ttccaagctc tacctcgagg gatccgtcaa cggacaccaa ttcaagtgca cccacgaggg
     1381 agagggaaag ccatacgagg gaaagcaaac caaccgtatc aaggtcgtcg agggaggacc
     1441 actcccattc gccttcgaca tcctcgccac ccacttcatg tacggatcca aggtaagttt
     1501 aaacatatat atactaacta accctgatta tttaaatttt caggtcttca tcaagtaccc
     1561 agccgacctc ccagactact tcaagcaatc cttcccagag ggattcacct gggagcgtgt
     1621 catggtcttc gaggacggag gagtcctcac cgccacccaa gacacctccc tccaagacgg
     1681 agagctcatc tacaacgtca aggtccgtgg agtcaacttc ccagccaacg gaccagtcat
     1741 gcaaaagaag accctcggat gggagccatc caccgagacc atgtacccag ccgacggagg
     1801 actcgaggga cgttgcgaca aggtaagttt aaacatgatt ttactaacta actaatctga
     1861 tttaaatttt caggccctca agctcgtcgg aggaggacac ctccacgtca acttcaagac
     1921 cacctacaag tccaagaagc cagtcaagat gccaggagtc cactacgtcg accgtcgtct
     1981 cgagcgtatc aaggaggccg acaacgagac ctacgtcgag caatacgagc acgccgtcgc
     2041 ccgttactcc aacctcggag gaggaatgga cgagctctac aagcctagga taccggtggg
     2101 tgaagaccca ccaaagccat ctgccaaggg agccaagaag gccgccaaga ccgttacgaa
     2161 gccaaaggac ggaaagaaga gacgtcatgc ccgtaaggaa tcatactccg tctacatcta
     2221 ccgtgtcctc aagcaagttc atccagacac tggagtttcc tccaaagcca tgtctatcat
     2281 gaactctttt gtcaacgatg tcttcgagcg tattgctgct gaagcatccc gtcttgctca
     2341 ctacaacaag cgttccacaa tctcatcccg cgaaattcag accgctgtcc gtctgatcct
     2401 tccaggagag cttgccaagc acgccgtgtc tgagggaacc aaggccgtta ccaagtacac
     2461 ttccagcaag taggaattcc aactgagcgc cggtcgctac cattaccaac ttgtctggtg
     2521 tcaaaaataa taggggccgc tgtcatcaga gtaagtttaa actgagttct actaactaac
     2581 gagtaatatt taaattttca gcatctcgcg cccgtgcctc tgacttctaa gtccaattac
     2641 tcttcaacat ccctacatgc tctttctccc tgtgctccca ccccctattt ttgttattat
     2701 caaaaaactt ctcttaattt ctttgttttt tagcttcttt taagtcacct ctaacaatga
     2761 aattgtgtag attcaaaaat agaattaatt cgtaataaaa agtcgaaaaa aattgtgctc
     2821 cctcccccca ttaataataa ttctatccca aaatctacac aatgttctgt gtacacttct
     2881 tatgtttttt acttctgata aatttttttg aaacatcata gaaaaaaccg cacacaaaat
     2941 accttatcat atgttacgtt tcagtttatg accgcaattt ttatttcttc gcacgtctgg
     3001 gcctctcatg acgtcaaatc atgctcatcg tgaaaaagtt ttggagtatt tttggaattt
     3061 ttcaatcaag tgaaagttta tgaaattaat tttcctgctt ttgctttttg gggtttcccc
     3121 tattgtttgt caagatttcg aggacggcgt ttttcttgct aaaatcacaa gtattgatga
     3181 gcacgatgca agaaagatcg gaagaaggtt tgggtttgag gctcagtgga aggtgagtag
     3241 aagttgataa tttgaaagtg gagtagtgtc tatggggttt ttgccttaaa tgacagaata
     3301 cattcccaat ataccaaaca taactgtttc ctactagtcg gccgtacggg ccctttcgtc
     3361 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
     3421 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
     3481 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
     3541 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcggc
     3601 cttaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
     3661 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     3721 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     3781 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     3841 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     3901 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     3961 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     4021 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     4081 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     4141 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     4201 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     4261 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     4321 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     4381 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     4441 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     4501 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     4561 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     4621 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     4681 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     4741 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     4801 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     4861 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     4921 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     4981 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     5041 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     5101 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     5161 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     5221 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     5281 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     5341 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     5401 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     5461 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     5521 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     5581 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     5641 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
     5701 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
     5761 gctcgtatgt tgtgtggaat tgtgagcgga taacaatt
//
