LOCUS       sequence-376038-       10814 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For use in RBP tethering screen.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10814)
  AUTHORS   Luo EC, Nathanson JL, Tan FE, Schwartz JL, Schmok JC, Shankar A, 
            Markmiller S, Yee BA, Sathe S, Pratt GA, Scaletta DB, Ha Y, Hill DE,
            Aigner S, Yeo GW
  TITLE     Large-scale tethered function assays identify factors that regulate 
            mRNA stability and translation.
  JOURNAL   Nat Struct Mol Biol. 2020 Oct;27(10):989-1000. doi: 
            10.1038/s41594-020-0477-6. Epub 2020 Aug 17.
  PUBMED    32807991
REFERENCE   2  (bases 1 to 10814)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41594-020-0477-6"; journalName: "Nat Struct Mol Biol"; 
            date: "2020-10"; volume: "27"; issue: "10"; pages: "989-1000"
COMMENT     Sequence Label: CRKRS
FEATURES             Location/Qualifiers
     source          1..10814
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        101..1279
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          332..1270
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1227..1247
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     1296..1315
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1296..1314
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1393..1417
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5871..5895)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6020..6061
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(6516..6533)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6522..6746
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6879..6898)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    7030..7077
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             7085..8107
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             7085..8107
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    8237..8358
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8274..8293)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8328..8347
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8407..8423)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8407..8423)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8420..8442)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8431..8447
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8455..8461,8462..8479,8480..8485))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8500..8521
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8638..8655)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8809..9397)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8889..8908)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9568..10359,10360..10428))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10191..10210
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10429..10533)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10608..10627)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 taggcttttg caaaaagctt tgcaaagatg gataaagttt taaacagaga ggaatctttg
       61 cagctaatgg accttctagg tcttgaaagg agtgggaatt ggctccggtg cccgtcagtg
      121 ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac
      181 cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg
      241 cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct
      301 ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt cccgcgggcc
      361 tggcctcttt acgggttatg gcccttgcgt gccttgaatt acttccacct ggctgcagta
      421 cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg gagagttcga ggccttgcgc
      481 ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct ggggccgccg
      541 cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt ctctagccat
      601 ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg caagatagtc ttgtaaatgc
      661 gggccaagat ctgcacactg gtatttcggt ttttggggcc gcgggcggcg acggggcccg
      721 tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac cgagaatcgg
      781 acgggggtag tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc cgccgtgtat
      841 cgccccgccc tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag cggaaagatg
      901 gccgcttccc ggccctgctg cagggagctc aaaatggagg acgcggcgct cgggagagcg
      961 ggcgggtgag tcacccacac aaaggaaaag ggcctttccg tcctcagccg tcgcttcatg
     1021 tgactccacg gagtaccggg cgccgtccag gcacctcgat tagttctcga gcttttggag
     1081 tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg gagtttcccc acactgagtg
     1141 ggtggagact gaagttaggc cagcttggca cttgatgtaa ttctccttgg aatttgccct
     1201 ttttgagttt ggatcttggt tcattctcaa gcctcagaca gtggttcaaa gtttttttct
     1261 tccatttcag gtgtcgtgag gaattagctt ggtactaata cgactcacta tagggagacc
     1321 caagctggct aggtaagctt ggtaccgagc tcggatccac tagtccagtg tggtggaatt
     1381 ctgcagatat caacaagttt gtacaaaaaa gcaggctgcc accatgccca attcagagag
     1441 acatgggggc aagaaggacg ggagtggagg agcttctgga actttgcagc cgtcatcggg
     1501 aggcggcagc tctaacagca gagagcgtca ccgcttggta tcgaagcaca agcggcataa
     1561 gtccaaacac tccaaagaca tggggttggt gacccccgaa gcagcatccc tgggcacagt
     1621 tatcaaacct ttggtggagt atgatgatat cagctctgat tccgacacct tctccgatga
     1681 catggccttc aaactagacc gaagggagaa cgacgaacgt cgtggatcag atcggagcga
     1741 tcgcctgcac aaacatcgtc accaccagca caggcgttcc cgggacttac taaaagctaa
     1801 acagaccgaa aaagaaaaaa gccaagaagt ctccagcaag tcgggatcga tgaaggaccg
     1861 gatatcggga agttcaaagc gttcgaatga ggagactgat gactatggga aggcgcaggt
     1921 agccaaaagc agcagcaagg aatccaggtc atccaagctc cacaaggaga agaccaggaa
     1981 agaacgggag ctgaagtctg ggcacaaaga ccggagtaaa agtcatcgaa aaagggaaac
     2041 acccaaaagt tacaaaacag tggacagccc aaaacggaga tccaggagcc cccacaggaa
     2101 gtggtctgac agctccaaac aagatgatag cccctcggga gcttcttatg gccaagatta
     2161 tgaccttagt ccctcacgat ctcatacctc gagcaattat gactcctaca agaaaagtcc
     2221 tggaagtacc tcgagaaggc agtcggtcag tcccccttac aaggagcctt cggcctacca
     2281 gtccagcacc cggtcaccga gcccctacag taggcgacag agatctgtca gtccctatag
     2341 caggagacgg tcgtccagct acgaaagaag tggctcttac agcgggcgat cgcccagtcc
     2401 ctatggtcga aggcggtcca gcagcccctt cctgagcaag cggtctctga gtcggagtcc
     2461 actccccagt aggaaatcca tgaagtccag aagtagaagt cctgcatatt caagacattc
     2521 atcttctcat agtaaaaaga agagatccag ttcacgcagt cgtcattcca gtatctcacc
     2581 tgtcaggctt ccacttaatt ccagtctggg agctgaactc agtaggaaaa agaaggaaag
     2641 agcagctgct gctgctgcag caaagatgga tggaaaggag tccaagggtt cacctgtatt
     2701 tttgcctaga aaagagaaca gttcagtaga ggctaaggat tcaggtttgg agtctaaaaa
     2761 gttacccaga agtgtaaaat tggaaaaatc tgccccagat actgaactgg tgaatgtaac
     2821 acatctaaac acagaggtaa aaaattcttc agatacaggg aaagtaaagt tggatgagaa
     2881 ctccgagaag catcttgtta aagatttgaa agcacaggga acaagagact ctaaacccat
     2941 agcactgaaa gaggagattg ttactccaaa ggagacagaa acatcagaaa aggagacccc
     3001 tccacctctt cccacaattg cttctccccc accccctcta ccaactacta cccctccacc
     3061 tcagacaccc cctttgccac ctttgcctcc aataccagct cttccacagc aaccacctct
     3121 gcctccttct cagccagcat ttagtcaggt tcctgcttcc agtacttcaa ctttgccccc
     3181 ttctactcac tcaaagacat ctgctgtgtc ctctcaggca aattctcagc cccctgtaca
     3241 ggtttctgtg aagactcaag tatctgtaac agctgctatt ccacacctga aaacttcaac
     3301 gttgcctcct ttgcccctcc cacccttatt acctggagat gatgacatgg atagtccaaa
     3361 agaaactctt ccttcaaaac ctgtgaagaa agagaaggaa cagaggacac gccacttact
     3421 cacagacctt cctctccctc cagagctccc tggtggagat ctgtctcccc cagactctcc
     3481 agaaccaaag gcaatcacac cacctcagca accatataaa aagagaccaa aaatttgttg
     3541 tcctcgttat ggagaaagaa gacaaacaga aagcgactgg gggaaacgct gtgtggacaa
     3601 gtttgacatt attgggatta ttggagaagg aacctatggc caagtatata aagccaagga
     3661 caaagacaca ggagaactag tggctctgaa gaaggtgaga ctagacaatg agaaagaggg
     3721 cttcccaatc acagccattc gtgaaatcaa aatccttcgt cagttaatcc accgaagtgt
     3781 tgttaacatg aaggaaattg tcacagataa acaagatgca ctggatttca agaaggacaa
     3841 aggtgccttt taccttgtat ttgagtatat ggaccatgac ttaatgggac tgctagaatc
     3901 tggtttggtg cacttttctg aggaccatat caagtcgttc atgaaacagc taatggaagg
     3961 attggaatac tgtcacaaaa agaatttcct gcatcgggat attaagtgtt ctaacatttt
     4021 gctgaataac agtgggcaaa tcaaactagc agattttgga cttgctcggc tctataactc
     4081 tgaagagagt cgcccttaca caaacaaagt cattactttg tggtaccgac ctccagaact
     4141 actgctagga gaggaacgtt acacaccagc catagatgtt tggagctgtg gatgtattct
     4201 tggggaacta ttcacaaaga agcctatttt tcaagccaat ctggaactgg ctcagctaga
     4261 actgatcagc cgactttgtg gtagcccttg tccagctgtg tggcctgatg ttatcagact
     4321 gccctacttc aacaccatga aaccgaagaa gcaatatcga aggcgtctac gagaagaatt
     4381 ctctttcatt ccttctgcag cacttgattt attggaccac atgctgacac tagatcctag
     4441 taagcggtgc acagctgaac agaccctaca gagcgacttc cttaaagatg tcgaactcag
     4501 caaaatggct cctccagacc tcccccactg gcaggattgc catgagttgt ggagtaagaa
     4561 acggcgacgt cagcgacaaa gtggtgttgt agtcgaagag ccacctccat ccaaaacttc
     4621 tcgaaaagaa actacctcag ggacaagtac tgagcctgtg aagaacagca gcccagcacc
     4681 acctcagcct gctcctggca aggtggagtc tggggctggg gatgcaatag gccttgctga
     4741 catcacacaa cagctgaatc aaagtgaatt ggcagtgtta ttaaacctgc tgcagagcca
     4801 aaccgacctg agcatccctc aaatggcaca gctgcttaac atccactcca acccagagat
     4861 gcagcagcag ctggaagccc tgaaccaatc catcagtgcc ctgacggaag ctacttccca
     4921 gcagcaggac tcagagacca tggccccaga ggagtctttg aaggaagcac cctctgcccc
     4981 agtgatcctg ccttcagcag aacagacgac ccttgaagct tcaagcacac cagctgacat
     5041 gcagaatata ttggcagttc tcttgagtca gctgatgaaa acccaagagc cagcaggcag
     5101 tctggaggaa aacaacagtg acaagaacag tgggccacag gggccccgaa gaactcccac
     5161 aatgccacag gaggaggcag cagagaagag gccccctgag ccccccggac ctccaccgcc
     5221 gccacctcta ccccctctgg ttgaaggcga tctttccagc gccccccagg agttgaaccc
     5281 agccgtgaca gccgccttgc tgcaactttt atcccagcct gaagcagagc ctcctggcca
     5341 cctgccacat gagcaccagg ccttgagacc aatggagtac tccacccgac cccgtccaaa
     5401 caggacttat ggaaacactg atgggcctga aacagggttc agtgccattg acactgatga
     5461 acgaaactct ggtccagcct tgacagaatc cttggtccag accctggtga agaacaggac
     5521 cttctcaggc tctctgagcc accttgggga gtccagcagt taccagggca cagggtcagt
     5581 gcagtttcca ggggaccagg acctccgttt tgccagggtc cccttagcgt tacacccggt
     5641 ggtcgggcaa ccattcctga aggctgaggg aagcagcaat tctgtggtac atgcagagac
     5701 caaattgcaa aactatgggg agctggggcc aggaaccact ggggccagca gctcaggagc
     5761 aggccttcac tgggggggcc caactcagtc ttctgcttat ggaaaactct atcgggggcc
     5821 tacaagagtc ccaccaagag ggggaagagg gagaggagtt ccttacttgg acccagcttt
     5881 cttgtacaaa gtggttgata tccagcacag tggcggccgc tcgagtctag agggcccgcg
     5941 gttcgaaacc agcggaggcg gcggaagcgg gggaggaggc agcggcggag gcggcagcgg
     6001 gggcggcgga agcgctaccg gtaagcctat ccctaaccct ctcctcggtc tcgattctac
     6061 gcgtaccggt atggcttcta actttactca gttcgttctc gtcgacaatg gcggaactgg
     6121 cgacgtgact gtcgccccaa gcaacttcgc taacggggtc gctgaatgga tcagctctaa
     6181 ctcgcgatca caggcttaca aagtaacctg tagcgttcgt cagagctctg cgcagaatcg
     6241 caaatacacc atcaaagtcg aggtgcctaa agtggcaacc cagactgttg gtggtgaaga
     6301 gcttcctgta gccggatgga gatcttactt aaatatggaa ctaaccattc caattttcgc
     6361 cacgaattcc gactgcgagc ttattgttaa ggcaatgcaa ggtctcctaa aagatggaaa
     6421 cccgattccc tcggccatcg cggccaactc cggcatctac ggaggtggag gtggatctcg
     6481 tacgggttag taatgagttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca
     6541 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
     6601 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
     6661 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
     6721 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag
     6781 ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     6841 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     6901 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     6961 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     7021 acgtacctag aagttcctat tccgaagttc ctattctcta gaaagtatag gaacttcctt
     7081 ggccaaaaag cctgaactca ccgcgacgtc tgtcgagaag tttctgatcg aaaagttcga
     7141 cagcgtctcc gacctgatgc agctctcgga gggcgaagaa tctcgtgctt tcagcttcga
     7201 tgtaggaggg cgtggatatg tcctgcgggt aaatagctgc gccgatggtt tctacaaaga
     7261 tcgttatgtt tatcggcact ttgcatcggc cgcgctcccg attccggaag tgcttgacat
     7321 tggggaattc agcgagagcc tgacctattg catctcccgc cgtgcacagg gtgtcacgtt
     7381 gcaagacctg cctgaaaccg aactgcccgc tgttctgcag ccggtcgcgg aggccatgga
     7441 tgcgatcgct gcggccgatc ttagccagac gagcgggttc ggcccattcg gaccgcaagg
     7501 aatcggtcaa tacactacat ggcgtgattt catatgcgcg attgctgatc cccatgtgta
     7561 tcactggcaa actgtgatgg acgacaccgt cagtgcgtcc gtcgcgcagg ctctcgatga
     7621 gctgatgctt tgggccgagg actgccccga agtccggcac ctcgtgcacg cggatttcgg
     7681 ctccaacaat gtcctgacgg acaatggccg cataacagcg gtcattgact ggagcgaggc
     7741 gatgttcggg gattcccaat acgaggtcgc caacatcttc ttctggaggc cgtggttggc
     7801 ttgtatggag cagcagacgc gctacttcga gcggaggcat ccggagcttg caggatcgcc
     7861 gcggctccgg gcgtatatgc tccgcattgg tcttgaccaa ctctatcaga gcttggttga
     7921 cggcaatttc gatgatgcag cttgggcgca gggtcgatgc gacgcaatcg tccgatccgg
     7981 agccgggact gtcgggcgta cacaaatcgc ccgcagaagc gcggccgtct ggaccgatgg
     8041 ctgtgtagaa gtactcgccg atagtggaaa ccgacgcccc agcactcgtc cgagggcaaa
     8101 ggaatagcac gtactacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt
     8161 cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga
     8221 gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     8281 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     8341 actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta
     8401 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     8461 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     8521 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     8581 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     8641 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     8701 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     8761 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     8821 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     8881 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     8941 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     9001 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     9061 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     9121 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     9181 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     9241 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     9301 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     9361 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     9421 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     9481 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     9541 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     9601 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     9661 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     9721 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     9781 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     9841 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     9901 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     9961 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
    10021 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
    10081 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
    10141 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
    10201 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
    10261 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
    10321 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
    10381 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
    10441 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
    10501 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
    10561 cgtcgacgga tcgggagatc tcccgatccc ctatggtgca ctctcagtac aatctgctct
    10621 gatgccgcat agttaagcca gtatctgctc cctgcttgtg tgttggaggt cgctgagtag
    10681 tgcgcgagca aaatttaagc tacaacaagg caaggcttga ccgacaattg catgaagaat
    10741 ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac gggccagata tacgcgttga
    10801 cattgattat tgac
//
