LOCUS       sequence-376039-        5561 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  dhc-3 fluorescent neural reporter driving nuclear mNeptune2.5 
            expression (refer to NeuroPAL paper for expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5561)
  AUTHORS   Yemini E, Lin A, Nejatbakhsh A, Varol E, Sun R, Mena GE, Samuel ADT,
            Paninski L, Venkatachalam V, Hobert O
  TITLE     NeuroPAL: A Multicolor Atlas for Whole-Brain Neuronal Identification
            in C. elegans.
  JOURNAL   Cell. 2021 Jan 7;184(1):272-288.e11. doi: 
            10.1016/j.cell.2020.12.012. Epub 2020 Dec 29.
  PUBMED    33378642
REFERENCE   2  (bases 1 to 5561)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.012"; journalName: "Cell"; date: "2021-01-7-
            7"; volume: "184"; issue: "1"; pages: "272-288.e11"
COMMENT     Sequence Label: pEY42
FEATURES             Location/Qualifiers
     source          1..5561
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6..22
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1236..1256
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1281..1301
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1323..1343
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(3152..3174)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3274..3293
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(3371..3389)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3457..3561
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3562..3630,3631..4422)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3780..3799)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4593..5181
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5082..5101
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    5469..5490
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5505..5510,5511..5528,5529..5535)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5543..5559
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(5548..5561,1..9)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcctgcaga ccacatatcg
      181 catcgtcgga aatgattact tttgcaatca tttaaataga atgtgcgacg cccactgaaa
      241 tatggattat ttgacactct tatcttaata attggaaaaa aaagtgttat cagtaattga
      301 tgatgatacg acttaaagat gacgaatggg aaaatcacac tttaagctgt ttaatattca
      361 aataaatata acattattgt gattaaaacg tgaatttccg gaggttaaat ttcataagaa
      421 gagacgaata atattcagag ctagggctgt tataggggac gaggggggac gtccccctcg
      481 attattgaat gctttttccg gcgtcccttt tgtcggaaac tgaagcccca aaatcaaatc
      541 gaagtaatat ccataatatt aatccaaatt gattttgggg ctccagtttc cgacaaaagg
      601 gacgcgggaa aaagccctca aaaatccagg gggacgacca ccccctattc agagcacaag
      661 tgttaaagct ccacggcgac gcctcatccc cgcccaaact ttgctgcgta tttttgtgaa
      721 ttgtcttttt aatctcattt tttcagctgc gtgttccgag agagaggata aaattttcct
      781 gtggaatctc tgcagagatt gatacttttt aatcgacaaa aatgcatcat tttgcatttt
      841 aaataaattt ctttccattt tgcatacatt tgtctatgta gagttagaaa agatttcttc
      901 aactttcagc ctatgtcact taacaagagt ctatatttta aatgtttaac acacattgga
      961 agttcgacca aacattgttg cacctccaca ttttcagtgg cccactattt caaatcggaa
     1021 gtgaaacatc atgcttcaca acacatgcca tctatggcac ctatgaacac atttgatttc
     1081 tcgcccagca ccatcagccc gggattggcc aaaggaccca aaggtatgtt tcgaatgata
     1141 ctaacataac atagaacatt ttcaggagga cccttggagg gtaccggttt tcccagtcac
     1201 gacgcgtggt accgagctca gaaaaaatga ccgctccaaa gaagaaacgc aaagtaccga
     1261 gctcagaaaa aatgacggca cctaaaaaga aacgcaaagt accggtcgcc gagaagacag
     1321 ccccaaaaaa gaagcgtaaa gttccacgcg tcatgggagt aagtttaaac agttcggtac
     1381 taactaacca tacatattta aattttcagc gcgctgtctc caagggagag gagctcatca
     1441 aggagaacat gcacaccaag ctctacatgg agggaaccgt caacaaccac cacttcaagt
     1501 gcacccacga aggggagggc aagccctacg agggcaccca gaccaacagg attaaggtgg
     1561 tggagggagg ccccctgccg ttcgcattcg acatcctggc cacctgcttt atgtacggga
     1621 gcaagacctt catcaaccac acccaaggaa tcccagactt cttcaagcaa tccttcgtaa
     1681 gtttaaacat gattttacta actaactaat ctgatttaaa ttttcagcct gagggcttca
     1741 catgggagag agtcaccaca tacgaagacg ggggcgtgct taccgttacc caggacacca
     1801 gcctccagga cggctgcttg atctacaacg tcaagctcag aggggtgaac ttcccatcca
     1861 acggccctgt gatgcagaag aaaacactcg gctgggaggc cagtaccgag accctgtacc
     1921 ccgctgacgg cggcctggaa ggcagatgcg acatggccct gaagctcgtg ggcgggggcc
     1981 acctgcactg caacctgaag accacataca gatccaagaa acccgctaag aacctcaaga
     2041 tgccaggagt aagtttaaac atatatatac taactaaccc tgattattta aattttcagg
     2101 tctactttgt ggaccgcaga ctggaaagaa tcaaggaggc cgacaatgag acctacgtcg
     2161 agcagcacga ggtggctgtg gccagatact gcgacctccc tagcaaactg gggcacaaac
     2221 ttaatggcat ggacgagctg tacaagtaag cgccggtcgc taccattacc aacttgtctg
     2281 gtgtcaaaaa taataggggc cgctgtcatc agagtaagtt taaactgagt tctactaact
     2341 aacgagtaat atttaaattt tcagcatctc gcgcccgtgc ctctgacttc taagtccaat
     2401 tactcttcaa catccctaca tgctctttct ccctgtgctc ccacccccta tttttgttat
     2461 tatcaaaaaa cttctcttaa tttctttgtt ttttagcttc ttttaagtca cctctaacaa
     2521 tgaaattgtg tagattcaaa aatagaatta attcgtaata aaaagtcgaa aaaaattgtg
     2581 ctccctcccc ccattaataa taattctatc ccaaaatcta cacaatgttc tgtgtacact
     2641 tcttatgttt tttacttctg ataaattttt ttgaaacatc atagaaaaaa ccgcacacaa
     2701 aataccttat catatgttac gtttcagttt atgaccgcaa tttttatttc ttcgcacgtc
     2761 tgggcctctc atgacgtcaa atcatgctca tcgtgaaaaa gttttggagt atttttggaa
     2821 tttttcaatc aagtgaaagt ttatgaaatt aattttcctg cttttgcttt ttggggtttc
     2881 ccctattgtt tgtcaagatt tcgaggacgg cgtttttctt gctaaaatca caagtattga
     2941 tgagcacgat gcaagaaaga tcggaagaag gtttgggttt gaggctcagt ggaaggtgag
     3001 tagaagttga taatttgaaa gtggagtagt gtctatgggg tttttgcctt aaatgacaga
     3061 atacattccc aatataccaa acataactgt ttcctactag tcggccgtac gggccctttc
     3121 gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg
     3181 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg
     3241 gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag
     3301 tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc
     3361 ggccttaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
     3421 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     3481 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     3541 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     3601 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     3661 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     3721 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     3781 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     3841 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     3901 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     3961 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     4021 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     4081 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     4141 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     4201 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     4261 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     4321 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     4381 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     4441 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     4501 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     4561 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     4621 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     4681 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     4741 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     4801 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     4861 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     4921 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     4981 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     5041 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     5101 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     5161 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     5221 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     5281 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     5341 tcagtgagcg atgaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
     5401 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
     5461 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt
     5521 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat t
//
