LOCUS       sequence-376040-        6281 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C15C8.4 fluorescent neural reporter driving nuclear mTagBFP2 
            expression (refer to NeuroPAL paper for expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6281)
  AUTHORS   Yemini E, Lin A, Nejatbakhsh A, Varol E, Sun R, Mena GE, Samuel ADT,
            Paninski L, Venkatachalam V, Hobert O
  TITLE     NeuroPAL: A Multicolor Atlas for Whole-Brain Neuronal Identification
            in C. elegans.
  JOURNAL   Cell. 2021 Jan 7;184(1):272-288.e11. doi: 
            10.1016/j.cell.2020.12.012. Epub 2020 Dec 29.
  PUBMED    33378642
REFERENCE   2  (bases 1 to 6281)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.012"; journalName: "Cell"; date: "2021-01-7-
            7"; volume: "184"; issue: "1"; pages: "272-288.e11"
COMMENT     Sequence Label: pEY66
FEATURES             Location/Qualifiers
     source          1..6281
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6..22
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1646..1666
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1688..1708
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(3872..3894)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3994..4013
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4091..4109)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4177..4281
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4282..4350,4351..5142)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4500..4519)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5313..5901
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5802..5821
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6055..6072
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6189..6210
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6225..6230,6231..6248,6249..6255)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6263..6279
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(6268..6281,1..9)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcctgcagc atggctgttg
      181 gcatctgtaa ttcaaatgga atctttaaaa tctgatcttg taatagttct taattttttt
      241 tgtagatttt ctgactataa ctaacaataa gaaggttcaa aatcttgcca gtgtattttt
      301 ggcaacttct cttgaaaatc atggttatac attttcacga ttagttccaa taacaaaata
      361 gccgaaatac aactttctat cgtattaact gaattattta gtctttgggg atgcagagaa
      421 aatcattaat gtattttcca tattagtcat acacccccga ttctgtgaac tatttttgat
      481 gattgatggt gcaaggaaaa atatggtata atacgtctac cctgttaatt tattggtaat
      541 gaaagtagag taatgaatgt taaacggctt tcaaaactaa ttaatatcaa aaaataacct
      601 accgtaaagc gccttgaaag tcactctcgc agttaaaaca ggttattgtt cctacaatta
      661 cagcgttgaa agtcaaaatg accaacaagg ggacaaataa catgttcttt cttctgattc
      721 ttctccttct tctatatttc agtggtttgt taacaagaaa tagaaagaat aactaactga
      781 agaagcccgt ctttttcaag ttccacactc atcgagaagg aggagacggg aaaacgacga
      841 tgaagattcg attgtcttct tctcgtcttt tgagcttttc gttgatttca actcgatttt
      901 cggatattca ttgcacgcaa ccctcttttc gttgtccttc ccacgaaatc aaattttcta
      961 ttcagctctc tttctctgtg tctctttttg tgctagaatt ttgatatgag atgacacaaa
     1021 tagagagaca atcaagaatg gctcattgag cacatttcaa atttcatttc cttgaattat
     1081 gttgccaaat ttacagtagg tacccaaatc aaaaagattt ttgaacggca ttatcttctg
     1141 aattttaatt atattctaga tctcggctac tgtaatttct acagtagtcc ctgttaaaat
     1201 tggaacactc aaaaactttg ggaggcacaa gttttcaaac gtttctcatt gttcgaacag
     1261 tgattaatct attacgaatg caaatcaatt aatctatcga tttgacgatc tttccgatta
     1321 ccttaatcta ttgccagaaa tctctgtaac ctcttcccat ttctcttcat ctccactctc
     1381 ttacatgtga ctgtggggcg cgcttgcatt gtacaaattt ctccattttt tattttcaaa
     1441 ttttactctt tcgaaagtgt ttcttttaaa ttttccgtta aattttttat gtttaataag
     1501 ctttaaatgt ctcaataatt acaattttca gcccgggatt ggccaaagga cccaaaggta
     1561 tgtttcgaat gatactaaca taacatagaa cattttcagg aggacccttg cttggagggt
     1621 accgagctca gaaaaaatga ccgctccaaa gaagaagcgc aaggttccag tcgagaagat
     1681 gactgcccca aaaaagaaac gtaaggtccc gcggatgtca gagcttatta aggagaatat
     1741 gcatatgaaa ctgtacatgg aaggaaccgt ggacaaccat cactttaagt gcacttcgga
     1801 gggggaaggc aaaccgtacg agggcaccca gaccatgcga atcaaggtgg tcgagggagg
     1861 cccactgccc ttcgccttcg acattctggt aagtttaaac atatatatac taactaaccc
     1921 tgattattta aattttcagg ctactagctt cctgtacggc agcaagactt ttattaatca
     1981 cacccagggc atcccggact tcttcaaaca gtcgttccct gagggcttca catgggagcg
     2041 tgtgaccaca tacgaagacg gtggcgttct caccgctacc caggacacca gcctccagga
     2101 cggttgcctc atatataatg tggtaagttt aaacagttcg gtactaacta accatacata
     2161 tttaaatttt cagaagatcc ggggagtaaa cttcacatcc aatggtccgg ttatgcagaa
     2221 gaaaacactc ggctgggagg ccttcaccga gacgctgtat ccggctgacg gaggcctgga
     2281 aggtcgaaac gacatggccc tgaagctcgt cggcggtagc catctcatcg caaacgctaa
     2341 gaccacatat agatccaaga aaccggtaag tttaaacatg attttactaa ctaactaatc
     2401 tgatttaaat tttcaggcta agaaccttaa aatgccggga gtctactatg ttgactacag
     2461 actggaacgg atcaaggagg ccaacaacga gacctacgtc gagcagcacg aggttgcagt
     2521 tgccagatac tgcgacctcc cgagcaaact gggtcacaag cttaatccta ggataccggt
     2581 gggtgaagac ccaccaaagc catctgccaa gggagccaag aaggccgcca agaccgttac
     2641 gaagccaaag gacggaaaga agagacgtca tgcccgtaag gaatcatact ccgtctacat
     2701 ctaccgtgtc ctcaagcaag ttcatccaga cactggagtt tcctccaaag ccatgtctat
     2761 catgaactct tttgtcaacg atgtcttcga gcgtattgct gctgaagcat cccgtcttgc
     2821 tcactacaac aagcgttcca caatctcatc ccgcgaaatt cagaccgctg tccgtctgat
     2881 ccttccagga gagcttgcca agcacgccgt gtctgaggga accaaggccg ttaccaagta
     2941 cacttccagc aagtaggaat tccaactgag cgccggtcgc taccattacc aacttgtctg
     3001 gtgtcaaaaa taataggggc cgctgtcatc agagtaagtt taaactgagt tctactaact
     3061 aacgagtaat atttaaattt tcagcatctc gcgcccgtgc ctctgacttc taagtccaat
     3121 tactcttcaa catccctaca tgctctttct ccctgtgctc ccacccccta tttttgttat
     3181 tatcaaaaaa cttctcttaa tttctttgtt ttttagcttc ttttaagtca cctctaacaa
     3241 tgaaattgtg tagattcaaa aatagaatta attcgtaata aaaagtcgaa aaaaattgtg
     3301 ctccctcccc ccattaataa taattctatc ccaaaatcta cacaatgttc tgtgtacact
     3361 tcttatgttt tttacttctg ataaattttt ttgaaacatc atagaaaaaa ccgcacacaa
     3421 aataccttat catatgttac gtttcagttt atgaccgcaa tttttatttc ttcgcacgtc
     3481 tgggcctctc atgacgtcaa atcatgctca tcgtgaaaaa gttttggagt atttttggaa
     3541 tttttcaatc aagtgaaagt ttatgaaatt aattttcctg cttttgcttt ttggggtttc
     3601 ccctattgtt tgtcaagatt tcgaggacgg cgtttttctt gctaaaatca caagtattga
     3661 tgagcacgat gcaagaaaga tcggaagaag gtttgggttt gaggctcagt ggaaggtgag
     3721 tagaagttga taatttgaaa gtggagtagt gtctatgggg tttttgcctt aaatgacaga
     3781 atacattccc aatataccaa acataactgt ttcctactag tcggccgtac gggccctttc
     3841 gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg
     3901 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg
     3961 gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag
     4021 tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc
     4081 ggccttaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
     4141 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     4201 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     4261 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     4321 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     4381 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     4441 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     4501 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     4561 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     4621 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     4681 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     4741 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     4801 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     4861 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     4921 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     4981 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     5041 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     5101 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     5161 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     5221 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     5281 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     5341 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     5401 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     5461 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     5521 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     5581 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     5641 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     5701 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     5761 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     5821 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     5881 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     5941 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     6001 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     6061 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
     6121 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
     6181 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt
     6241 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat t
//
