LOCUS       sequence-376041-        7318 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ceh-48 fluorescent neural reporter driving nuclear TagRFP-T 
            expression (refer to NeuroPAL paper for expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7318)
  AUTHORS   Yemini E, Lin A, Nejatbakhsh A, Varol E, Sun R, Mena GE, Samuel ADT,
            Paninski L, Venkatachalam V, Hobert O
  TITLE     NeuroPAL: A Multicolor Atlas for Whole-Brain Neuronal Identification
            in C. elegans.
  JOURNAL   Cell. 2021 Jan 7;184(1):272-288.e11. doi: 
            10.1016/j.cell.2020.12.012. Epub 2020 Dec 29.
  PUBMED    33378642
REFERENCE   2  (bases 1 to 7318)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.012"; journalName: "Cell"; date: "2021-01-7-
            7"; volume: "184"; issue: "1"; pages: "272-288.e11"
COMMENT     Sequence Label: pEY70
FEATURES             Location/Qualifiers
     source          1..7318
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6..22
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2922..2942
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2967..2987
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3009..3029
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(4909..4931)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5031..5050
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(5128..5146)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5214..5318
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5319..5387,5388..6179)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5537..5556)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6350..6938
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6839..6858
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7092..7109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7226..7247
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7262..7267,7268..7285,7286..7292)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7300..7316
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(7305..7318,1..9)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcctgcaga ggcagaagct
      181 cgaaagcatg tcgatgaaac tcaaaaagat ctgaacggtc tcgccgagtc gataattgaa
      241 actttgtgtg aagtctaggt cgaatgagca atgaacattt acattttgta taatatttac
      301 atgaccatct gtcctccatg gcttcgtgcc tggttcgcag gtcccttctg aatacaacta
      361 gggaaagagg aggaggaaaa ggaaaagcta catagaaccc tcgtcttttc aggcagtaag
      421 gccacaggaa aaatagtgag agaggcacgg agggagagac caatagaaga agaaataaga
      481 gatggggacc gccctttctc gggtggtacg tctctgcgtt tcctcgccac aaatggtatt
      541 tttatttcac agagagctct tcccgaagaa gggaaagcta gtagaaaagg agggtggctg
      601 tgcgtgtgtg caccagtttg tgcacagctt caaagcctaa atagacgcag gcagtaaaag
      661 gaaaaaagaa gacgaagaag gcgtcgtttg tatgtgtctc ttcatcccaa ataccccata
      721 caacaaccat tgccttcttc ccgtccagtt ccaacgcctt ttcacttttt ctctatatct
      781 ctagttcatg gcatttactt ttgcacattg gaggtctttg acgtctacag aaatttattt
      841 gtttcaatat gtctttagct aatttttaaa ctttaaagaa tttagtaaat aatattaatc
      901 cttgtcaaca agacaattgc cggcagacgg ctgggtctaa aatttagacc acggaattct
      961 atacagtgaa aaacatccac ttcaattatt ctggtcgttt tcttcagttc tcaatataaa
     1021 tttaagattt cgaagctagt tcagaatatg aatactttct aaactgctgt gattacagct
     1081 ttagaaaata caaaaattaa tttggaaatt gttctacata attctaaatc aagacattta
     1141 taatttaaaa tcaaagtttt cgcacagtga agttgagtac gcgaacaata attttaaatt
     1201 gtaacccaat attaaaacaa aaatgctttg ttaaaacttc aagaattagg ggtgcttgtg
     1261 aatcatagga cagaaagaat tcaatttttc aaatttaaat agtgcaattt tgttataagt
     1321 caaagtataa aataatttat aacaaaaaag acagtcaaat tggcttcttt aaatttatgt
     1381 ggcctgtagc aatcaacagg gaaaaaaatt taaaaaaaat actgtatgct gttgtttatt
     1441 tatttagagg tttttgaaat aatcttaatg atgatttcat atgtagtagt ttatatgtag
     1501 caaccgttcc agactacgca ttttctaaac tatcaaaaaa aaactaaatt aaaaaaatgc
     1561 ccaaataaag tttttagtag gcactccaag ttaccgtgat tgatggcaca aaacaaaaat
     1621 taatttttga tatcatgttc agtcaatcat aaacagaata ttcgtttttc ttccaatcaa
     1681 gaaagaaaag aaaaaaagaa ataggaaatc catcacctcc tttcgtttcc gttttctggg
     1741 aagagaaaaa ttaaaaaaga gatggaaacg aaaattgtca gttttccatt ccattttctg
     1801 cctatactat ttctcatttt tctttacaaa aaccaagtct tccatcaatc agtattttca
     1861 aaaaaaaaag tatgttattt caaaaggaaa aatacacttc gtaataccta cagttgactt
     1921 tatcattttt taatatttac gtgagcctag cttcagccta acggtagcca caagaatttt
     1981 acaattaaaa accacggatt ttttgcttaa aaaattactt ctagatttga gacctgctac
     2041 tgttctccgg gtaagtgaaa taaaattcta aattgaagtg ttcaatctga actgatcatg
     2101 aagtttcacg gtcatgataa aatttgtaaa attcatgtaa aatttgacac gtgtcgttat
     2161 ttcgtaaaag attatgtcgt aaaggtatat ttcaaaatgt cgtactaaat tgggaacttg
     2221 taaaattagc ggttagagaa gggataatgc gttttatttg ggtaaaactc ggcaattttt
     2281 ggcgtagttc ctctgccgct tgtaacgtca attttttcca aaaagaaagg cggtttcaac
     2341 atttttattt caattgataa ataaactaaa actgatggat attttgatca acgggaaatt
     2401 aaccagaaaa agcgtttttg atatccttta ggtagtcatt ggcaaccacg tagcttcaca
     2461 agcatgataa acaaactgat tcattttgtt ccaatattat ttctcccggc gacattatat
     2521 ttaggattgc taactagctg tagttattat aagtatggca gttttttgga aaagaacgat
     2581 ttttgtagca atttttgaaa tgatttttcc ttatacatga aaaaatcaga ctgtaggcaa
     2641 acttttcttc cttcaaaaaa aaaaacaatc aaaaaactgc tttcaattct caattcattc
     2701 attttgtctt catattccaa ttttttttca acttaatgtt tcaactcaaa caaaattaat
     2761 ttcagagcta cttcaaacga caacccggga ttggccaaag gacccaaagg tatgtttcga
     2821 atgatactaa cataacatag aacattttca ggaggaccct tggagggtac cggttttccc
     2881 agtcacgacg cgtggtaccg agctcagaaa aaatgaccgc tccaaagaag aaacgcaaag
     2941 taccgagctc agaaaaaatg acggcaccta aaaagaaacg caaagtaccg gtcgccgaga
     3001 agacagcccc aaaaaagaag cgtaaagttc cacgcgtcat gggagtaagt ttaaacagtt
     3061 cggtactaac taaccataca tatttaaatt ttcagcgcgc ttccaaggga gaggagctca
     3121 tcaaggagaa catgcacatg aagctctaca tggagggaac cgtcaacaac caccacttca
     3181 agtgcacctc cgagggagag ggaaagccat acgagggaac ccaaaccatg cgtatcaagg
     3241 taagtttaaa catatatata ctaactaacc ctgattattt aaattttcag gtcgtcgagg
     3301 gaggaccact cccattcgcc ttcgacatcc tcgccacctc cttcatgtac ggatcccgta
     3361 ccttcatcaa ccacacccaa ggaatcccag acttcttcaa gcaatccttc ccagagggat
     3421 tcacctggga gcgtgtcacc acctacgagg acggaggagt cctcaccgcc acccaagaca
     3481 cctccctcca agacggatgc ctcatctaca acgtcaaggt aagtttaaac agttcggtac
     3541 taactaacca tacatattta aattttcaga tccgtggagt caacttccca tccaacggac
     3601 cagtcatgca aaagaagacc ctcggatggg aggccaacac cgagatgctc tacccagccg
     3661 acggaggact cgagggacgt accgacatgg ccctcaagct cgtcggagga ggacacctca
     3721 tctgcaactt caagaccacc taccgttcca aggtaagttt aaacatgatt ttactaacta
     3781 actaatctga tttaaatttt cagaagccag ccaagaacct caagatgcca ggagtctact
     3841 acgtcgacca ccgtctcgag cgtatcaagg aggccgacaa ggagacctac gtcgagcaac
     3901 acgaggtcgc cgtcgcccgt tactgcgacc tcccatccaa gctcggacac aagctcaacg
     3961 gaatggacga gctctacaag gcctaggata ccggaattcc aactgagcgc cggtcgctac
     4021 cattaccaac ttgtctggtg tcaaaaataa taggggccgc tgtcatcaga gtaagtttaa
     4081 actgagttct actaactaac gagtaatatt taaattttca gcatctcgcg cccgtgcctc
     4141 tgacttctaa gtccaattac tcttcaacat ccctacatgc tctttctccc tgtgctccca
     4201 ccccctattt ttgttattat caaaaaactt ctcttaattt ctttgttttt tagcttcttt
     4261 taagtcacct ctaacaatga aattgtgtag attcaaaaat agaattaatt cgtaataaaa
     4321 agtcgaaaaa aattgtgctc cctcccccca ttaataataa ttctatccca aaatctacac
     4381 aatgttctgt gtacacttct tatgtttttt acttctgata aatttttttg aaacatcata
     4441 gaaaaaaccg cacacaaaat accttatcat atgttacgtt tcagtttatg accgcaattt
     4501 ttatttcttc gcacgtctgg gcctctcatg acgtcaaatc atgctcatcg tgaaaaagtt
     4561 ttggagtatt tttggaattt ttcaatcaag tgaaagttta tgaaattaat tttcctgctt
     4621 ttgctttttg gggtttcccc tattgtttgt caagatttcg aggacggcgt ttttcttgct
     4681 aaaatcacaa gtattgatga gcacgatgca agaaagatcg gaagaaggtt tgggtttgag
     4741 gctcagtgga aggtgagtag aagttgataa tttgaaagtg gagtagtgtc tatggggttt
     4801 ttgccttaaa tgacagaata cattcccaat ataccaaaca taactgtttc ctactagtcg
     4861 gccgtacggg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat
     4921 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg
     4981 tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga
     5041 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag
     5101 aaaataccgc atcaggcggc cttaagggcc tcgtgatacg cctattttta taggttaatg
     5161 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
     5221 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     5281 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     5341 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     5401 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     5461 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     5521 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     5581 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     5641 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     5701 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     5761 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     5821 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     5881 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     5941 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     6001 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     6061 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     6121 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     6181 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     6241 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     6301 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     6361 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     6421 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     6481 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     6541 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     6601 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     6661 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     6721 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     6781 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     6841 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     6901 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     6961 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     7021 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     7081 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     7141 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     7201 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
     7261 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatt
//
