LOCUS       sequence-376045-        5035 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  dat-1 fluorescent neural reporter driving nuclear mNeptune2.5 
            expression (refer to NeuroPAL paper for expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5035)
  AUTHORS   Yemini E, Lin A, Nejatbakhsh A, Varol E, Sun R, Mena GE, Samuel ADT,
            Paninski L, Venkatachalam V, Hobert O
  TITLE     NeuroPAL: A Multicolor Atlas for Whole-Brain Neuronal Identification
            in C. elegans.
  JOURNAL   Cell. 2021 Jan 7;184(1):272-288.e11. doi: 
            10.1016/j.cell.2020.12.012. Epub 2020 Dec 29.
  PUBMED    33378642
REFERENCE   2  (bases 1 to 5035)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.012"; journalName: "Cell"; date: "2021-01-7-
            7"; volume: "184"; issue: "1"; pages: "272-288.e11"
COMMENT     Sequence Label: pEY41
FEATURES             Location/Qualifiers
     source          1..5035
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6..22
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             710..730
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             755..775
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             797..817
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(2626..2648)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2748..2767
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(2845..2863)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2931..3035
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3036..3104,3105..3896)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3254..3273)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4067..4655
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4556..4575
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    4943..4964
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4979..4984,4985..5002,5003..5009)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5017..5033
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(5022..5035,1..9)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcctgcagc ctattccagt
      181 atgacccctt tgaagcagat ataatcgcac aaacatatac acatagctcg gataaatgta
      241 gaaaaagaag aaaagaagta taagtagata gatgctttcc ggcaattatc caccgcaccg
      301 tagtcttcac caactgagac tgcgtcgtta ggagacgccg acatgattca gaagcagaat
      361 ttggaagaaa aacgacgatg atattgaggc tggcacacat acaccggaat attcgacatg
      421 ccaccacatc tagattccaa ggcaatctct acctcttccc attctttcgg tttttttgtt
      481 ctgacaagaa aagtggatag ctacgggctc aatgagctga ttttattttt aaatatctta
      541 aaactatact agattcatgt gttttcaggg acccgggatt ggccaaagga cccaaaggta
      601 tgtttcgaat gatactaaca taacatagaa cattttcagg aggacccttg gagggtaccg
      661 gttttcccag tcacgacgcg tggtaccgag ctcagaaaaa atgaccgctc caaagaagaa
      721 acgcaaagta ccgagctcag aaaaaatgac ggcacctaaa aagaaacgca aagtaccggt
      781 cgccgagaag acagccccaa aaaagaagcg taaagttcca cgcgtcatgg gagtaagttt
      841 aaacagttcg gtactaacta accatacata tttaaatttt cagcgcgctg tctccaaggg
      901 agaggagctc atcaaggaga acatgcacac caagctctac atggagggaa ccgtcaacaa
      961 ccaccacttc aagtgcaccc acgaagggga gggcaagccc tacgagggca cccagaccaa
     1021 caggattaag gtggtggagg gaggccccct gccgttcgca ttcgacatcc tggccacctg
     1081 ctttatgtac gggagcaaga ccttcatcaa ccacacccaa ggaatcccag acttcttcaa
     1141 gcaatccttc gtaagtttaa acatgatttt actaactaac taatctgatt taaattttca
     1201 gcctgagggc ttcacatggg agagagtcac cacatacgaa gacgggggcg tgcttaccgt
     1261 tacccaggac accagcctcc aggacggctg cttgatctac aacgtcaagc tcagaggggt
     1321 gaacttccca tccaacggcc ctgtgatgca gaagaaaaca ctcggctggg aggccagtac
     1381 cgagaccctg taccccgctg acggcggcct ggaaggcaga tgcgacatgg ccctgaagct
     1441 cgtgggcggg ggccacctgc actgcaacct gaagaccaca tacagatcca agaaacccgc
     1501 taagaacctc aagatgccag gagtaagttt aaacatatat atactaacta accctgatta
     1561 tttaaatttt caggtctact ttgtggaccg cagactggaa agaatcaagg aggccgacaa
     1621 tgagacctac gtcgagcagc acgaggtggc tgtggccaga tactgcgacc tccctagcaa
     1681 actggggcac aaacttaatg gcatggacga gctgtacaag taagcgccgg tcgctaccat
     1741 taccaacttg tctggtgtca aaaataatag gggccgctgt catcagagta agtttaaact
     1801 gagttctact aactaacgag taatatttaa attttcagca tctcgcgccc gtgcctctga
     1861 cttctaagtc caattactct tcaacatccc tacatgctct ttctccctgt gctcccaccc
     1921 cctatttttg ttattatcaa aaaacttctc ttaatttctt tgttttttag cttcttttaa
     1981 gtcacctcta acaatgaaat tgtgtagatt caaaaataga attaattcgt aataaaaagt
     2041 cgaaaaaaat tgtgctccct ccccccatta ataataattc tatcccaaaa tctacacaat
     2101 gttctgtgta cacttcttat gttttttact tctgataaat ttttttgaaa catcatagaa
     2161 aaaaccgcac acaaaatacc ttatcatatg ttacgtttca gtttatgacc gcaattttta
     2221 tttcttcgca cgtctgggcc tctcatgacg tcaaatcatg ctcatcgtga aaaagttttg
     2281 gagtattttt ggaatttttc aatcaagtga aagtttatga aattaatttt cctgcttttg
     2341 ctttttgggg tttcccctat tgtttgtcaa gatttcgagg acggcgtttt tcttgctaaa
     2401 atcacaagta ttgatgagca cgatgcaaga aagatcggaa gaaggtttgg gtttgaggct
     2461 cagtggaagg tgagtagaag ttgataattt gaaagtggag tagtgtctat ggggtttttg
     2521 ccttaaatga cagaatacat tcccaatata ccaaacataa ctgtttccta ctagtcggcc
     2581 gtacgggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca
     2641 gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca
     2701 gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca
     2761 gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa
     2821 ataccgcatc aggcggcctt aagggcctcg tgatacgcct atttttatag gttaatgtca
     2881 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     2941 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     3001 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     3061 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     3121 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     3181 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     3241 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     3301 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     3361 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     3421 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     3481 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     3541 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     3601 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     3661 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     3721 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     3781 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     3841 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     3901 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     3961 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     4021 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     4081 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     4141 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     4201 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     4261 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     4321 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     4381 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     4441 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     4501 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     4561 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     4621 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     4681 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     4741 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     4801 ccgagcgcag cgagtcagtg agcgatgaag cggaagagcg cccaatacgc aaaccgcctc
     4861 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     4921 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     4981 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatt
//
