LOCUS       sequence-376046-        6514 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ocr-1 fluorescent neural reporter driving nuclear CYOFP expression 
            (refer to NeuroPAL paper for expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6514)
  AUTHORS   Yemini E, Lin A, Nejatbakhsh A, Varol E, Sun R, Mena GE, Samuel ADT,
            Paninski L, Venkatachalam V, Hobert O
  TITLE     NeuroPAL: A Multicolor Atlas for Whole-Brain Neuronal Identification
            in C. elegans.
  JOURNAL   Cell. 2021 Jan 7;184(1):272-288.e11. doi: 
            10.1016/j.cell.2020.12.012. Epub 2020 Dec 29.
  PUBMED    33378642
REFERENCE   2  (bases 1 to 6514)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.012"; journalName: "Cell"; date: "2021-01-7-
            7"; volume: "184"; issue: "1"; pages: "272-288.e11"
COMMENT     Sequence Label: pEY33
FEATURES             Location/Qualifiers
     source          1..6514
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6..22
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1873..1893
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1915..1935
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(4105..4127)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4227..4246
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4324..4342)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4410..4514
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4515..4583,4584..5375)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4733..4752)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5546..6134
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6035..6054
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6288..6305
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6422..6443
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6458..6463,6464..6481,6482..6488)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6496..6512
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(6501..6514,1..9)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcctgcagt ggaagaactc
      181 attacctgaa gaagtacaat taaattgaac ttgaaacaaa atataacggt tggttaaaaa
      241 aaaagaagaa atataatacc gtaattttat tttgcggaca tcgttacaaa aacagaaaaa
      301 ttaagacgcc cacaataatc aagatacaag attgcgagtg agaaaagaga gacctaattt
      361 tatgtaacat cacgagtttt cagccagcac ctggaatttt ttctgtgatt gaaaataaaa
      421 cacgtatata agcatcgcat tgtgataaca agaaaacctc ttcttcgcta ataatgggtc
      481 aacaagtcag agaaaagaga ctaaaaaccc gaaaaaagga atctacataa tatgcgttga
      541 cagcaacttt cggtcgaaat aatgaggtga aaggaatctg aagagatctg gagcacgcta
      601 cattcttccg cctactcgta ctttgagttc ttttgaatta ttatgcatgg acaagttcaa
      661 agggtccgag agactaaaaa gagaactgca gacaaaatta tgaataacat ttctcagata
      721 aagttcaatg aaaggttaga gtcataacat taggggtggg ctctaaacaa gaaaacttga
      781 agaaacaagg gaaagcaacg tccagaacgt gcttttctta aaaagaaaaa tgagatgagc
      841 tgaattttcc ggagagaatg ttttatgtgc acccaccaca cgtattttct acataaaaag
      901 aatgatttga tgtcatgatg aaggaaaata gaagtttcga agaaaacgta ccacagaaga
      961 atttgtttat ttcttcttaa gaaaaaagac aaaaaattag acaaagaaag acacaagaaa
     1021 cgttaaattt ggacccagta aaattttagt tttataattc ttgtgttctg tcgaaaatgt
     1081 atctattcac caaaaattat tattcttcct acttagcatt gtatgcagta aactattttt
     1141 gacacctaac tgtaaaatga gaaaaatata agttttcaaa ctagtattga gtgaagcaaa
     1201 accatactga aaaatgataa cttattgtga attaaattat atatatatgg acgcttttaa
     1261 tctgaaatta cccgttttcg tgcaactttt ctcaaatgcc gtggttgtgg agaaaaaata
     1321 ttaatggaat atttcgccaa aatctataaa aatagtaaat tctcaagttc tttttttctg
     1381 cagtttcacg acacaacacg ccataaaatt ctagcttaac taaaaaatat tgctggtttt
     1441 tctggatctc catgaacacc cttccgactt tttatcttat ttcactgcca atttccttat
     1501 tcaatccgaa aattgtgttt acatcttctg tcctctttcc aatctgtaat aaaatgtatt
     1561 cactggcgta ttcaattgta tttagaaaga caatccgaga atctggcgga gtataggtac
     1621 aaatcgagtg aggatggaga atacatttga gtcgagccag tcccttgtat ttgaaatcac
     1681 ggttttcttt atcagaagca catatttctc tgctttgata tcgttgtttt ttttttaatg
     1741 taaattcttg attcagaacc cgggattggc caaaggaccc aaaggtatgt ttcgaatgat
     1801 actaacataa catagaacat tttcaggagg acccttgctt ggagggtacc gagctcagaa
     1861 aaaatgaccg ctccaaagaa gaagcgcaag gttccagtcg agaagatgac tgccccaaaa
     1921 aagaaacgta aggtcccgcg ggtaagttta aacagttcgg tactaactaa ccatacatat
     1981 ttaaattttc agcgcgctgt gtcaaaagga gaagagctta ttaaggagaa tatgcgttcc
     2041 aagctctacc tcgagggatc cgtcaacgga caccaattca agtgcaccca cgagggagag
     2101 ggaaagccat acgagggaaa gcaaaccaac cgtatcaagg tcgtcgaggg aggaccactc
     2161 ccattcgcct tcgacatcct cgccacccac ttcatgtacg gatccaaggt aagtttaaac
     2221 atatatatac taactaaccc tgattattta aattttcagg tcttcatcaa gtacccagcc
     2281 gacctcccag actacttcaa gcaatccttc ccagagggat tcacctggga gcgtgtcatg
     2341 gtcttcgagg acggaggagt cctcaccgcc acccaagaca cctccctcca agacggagag
     2401 ctcatctaca acgtcaaggt ccgtggagtc aacttcccag ccaacggacc agtcatgcaa
     2461 aagaagaccc tcggatggga gccatccacc gagaccatgt acccagccga cggaggactc
     2521 gagggacgtt gcgacaaggt aagtttaaac atgattttac taactaacta atctgattta
     2581 aattttcagg ccctcaagct cgtcggagga ggacacctcc acgtcaactt caagaccacc
     2641 tacaagtcca agaagccagt caagatgcca ggagtccact acgtcgaccg tcgtctcgag
     2701 cgtatcaagg aggccgacaa cgagacctac gtcgagcaat acgagcacgc cgtcgcccgt
     2761 tactccaacc tcggaggagg aatggacgag ctctacaagc ctaggatacc ggtgggtgaa
     2821 gacccaccaa agccatctgc caagggagcc aagaaggccg ccaagaccgt tacgaagcca
     2881 aaggacggaa agaagagacg tcatgcccgt aaggaatcat actccgtcta catctaccgt
     2941 gtcctcaagc aagttcatcc agacactgga gtttcctcca aagccatgtc tatcatgaac
     3001 tcttttgtca acgatgtctt cgagcgtatt gctgctgaag catcccgtct tgctcactac
     3061 aacaagcgtt ccacaatctc atcccgcgaa attcagaccg ctgtccgtct gatccttcca
     3121 ggagagcttg ccaagcacgc cgtgtctgag ggaaccaagg ccgttaccaa gtacacttcc
     3181 agcaagtagg aattccaact gagcgccggt cgctaccatt accaacttgt ctggtgtcaa
     3241 aaataatagg ggccgctgtc atcagagtaa gtttaaactg agttctacta actaacgagt
     3301 aatatttaaa ttttcagcat ctcgcgcccg tgcctctgac ttctaagtcc aattactctt
     3361 caacatccct acatgctctt tctccctgtg ctcccacccc ctatttttgt tattatcaaa
     3421 aaacttctct taatttcttt gttttttagc ttcttttaag tcacctctaa caatgaaatt
     3481 gtgtagattc aaaaatagaa ttaattcgta ataaaaagtc gaaaaaaatt gtgctccctc
     3541 cccccattaa taataattct atcccaaaat ctacacaatg ttctgtgtac acttcttatg
     3601 ttttttactt ctgataaatt tttttgaaac atcatagaaa aaaccgcaca caaaatacct
     3661 tatcatatgt tacgtttcag tttatgaccg caatttttat ttcttcgcac gtctgggcct
     3721 ctcatgacgt caaatcatgc tcatcgtgaa aaagttttgg agtatttttg gaatttttca
     3781 atcaagtgaa agtttatgaa attaattttc ctgcttttgc tttttggggt ttcccctatt
     3841 gtttgtcaag atttcgagga cggcgttttt cttgctaaaa tcacaagtat tgatgagcac
     3901 gatgcaagaa agatcggaag aaggtttggg tttgaggctc agtggaaggt gagtagaagt
     3961 tgataatttg aaagtggagt agtgtctatg gggtttttgc cttaaatgac agaatacatt
     4021 cccaatatac caaacataac tgtttcctac tagtcggccg tacgggccct ttcgtctcgc
     4081 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc
     4141 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
     4201 cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca
     4261 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcggcctta
     4321 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
     4381 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     4441 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     4501 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     4561 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     4621 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     4681 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     4741 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     4801 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     4861 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     4921 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     4981 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     5041 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     5101 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     5161 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     5221 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     5281 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     5341 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     5401 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     5461 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     5521 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     5581 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     5641 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     5701 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     5761 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     5821 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     5881 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     5941 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     6001 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     6061 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     6121 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     6181 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     6241 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     6301 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     6361 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     6421 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
     6481 gtatgttgtg tggaattgtg agcggataac aatt
//
