LOCUS       sequence-376049-        5846 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ceh-2 fluorescent neural reporter driving nuclear mNeptune2.5 
            expression (refer to NeuroPAL paper for expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5846)
  AUTHORS   Yemini E, Lin A, Nejatbakhsh A, Varol E, Sun R, Mena GE, Samuel ADT,
            Paninski L, Venkatachalam V, Hobert O
  TITLE     NeuroPAL: A Multicolor Atlas for Whole-Brain Neuronal Identification
            in C. elegans.
  JOURNAL   Cell. 2021 Jan 7;184(1):272-288.e11. doi: 
            10.1016/j.cell.2020.12.012. Epub 2020 Dec 29.
  PUBMED    33378642
REFERENCE   2  (bases 1 to 5846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.012"; journalName: "Cell"; date: "2021-01-7-
            7"; volume: "184"; issue: "1"; pages: "272-288.e11"
COMMENT     Sequence Label: pEY40
FEATURES             Location/Qualifiers
     source          1..5846
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6..22
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1521..1541
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1566..1586
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1608..1628
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(3437..3459)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3559..3578
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(3656..3674)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3742..3846
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3847..3915,3916..4707)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4065..4084)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4878..5466
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5367..5386
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    5754..5775
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5790..5795,5796..5813,5814..5820)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5828..5844
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(5833..5846,1..9)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcctgcagg gtaaacccac
      181 ccattcgttt ctgttcaaaa attctcgcat attcaacaat aacaacttct ttgttaccct
      241 tctaatgacc tatttattat aacaataata atgaataaat atgggcgcta gctcagctca
      301 cggcgagtca ggttacctcc gccgagtctg gtacacaatt acataatctc gatgtcgtca
      361 gtgtctatcc aacgtagagg ttgaatctat tatataataa caatcttgta tgctgttcgg
      421 aaattattgc tctctgaaaa gttgatttaa gcctttttga tttgaaaacc ccaaagagtg
      481 acatttgata ttccacctgc tgctctacac acttcgctcc tgttgctgtc gtcggagaga
      541 tggccgagcg gtccaaggcg ctggtttaag gcaccagtcc cttcgggggc gtgggttcga
      601 atcccactct cttcagtttc tttttgaaca attttataaa ttttgtgctc atctcgtgct
      661 cacttcacct cgattactaa cataatatac ctatttctga gcccaacgtc cgctgctttc
      721 gggcgcacaa aagatgtaat ccgagatctg cgatttgtac atatttcgga tacattttca
      781 atctccattt ccaactcatc ttctaattcg ggacaatgag catcatcatg agcatgtgta
      841 cacaaggtag ttcggtcgcc cagttgccga ttacataggc acccggttgc cgagtagacg
      901 agttgtaaaa acagggcacc acgagatatt gccgatttcc acataaacta cttattaaag
      961 tctggagatt taattcatat gtcaagaaaa atgttcgtga aagcaacaat atatgtacta
     1021 gaaatcccta agacgacgac tatcgctgac gttacattgc tccaaaagaa tgcaatgaat
     1081 tgtgattaca ccatgagagt tttcaaaact ttaaacctca aattaatatt tcaacttatt
     1141 aacctacttc ctattctttt caattcttta tttgtctatc gtctctttat tcccaatgat
     1201 ctctcttcta agagcttcta ccttatcggc tactgtgtcc aattacccat cgacgctcct
     1261 ccctcttttg cgcgaaactg ggcggagcct aagatgagat tcagtaaaat aggttctcat
     1321 ttgggaaatc aacttaccct ttttggctag tttacttatt ttttctaata ttcggagtga
     1381 aacccgggat tggccaaagg acccaaaggt atgtttcgaa tgatactaac ataacataga
     1441 acattttcag gaggaccctt ggagggtacc ggttttccca gtcacgacgc gtggtaccga
     1501 gctcagaaaa aatgaccgct ccaaagaaga aacgcaaagt accgagctca gaaaaaatga
     1561 cggcacctaa aaagaaacgc aaagtaccgg tcgccgagaa gacagcccca aaaaagaagc
     1621 gtaaagttcc acgcgtcatg ggagtaagtt taaacagttc ggtactaact aaccatacat
     1681 atttaaattt tcagcgcgct gtctccaagg gagaggagct catcaaggag aacatgcaca
     1741 ccaagctcta catggaggga accgtcaaca accaccactt caagtgcacc cacgaagggg
     1801 agggcaagcc ctacgagggc acccagacca acaggattaa ggtggtggag ggaggccccc
     1861 tgccgttcgc attcgacatc ctggccacct gctttatgta cgggagcaag accttcatca
     1921 accacaccca aggaatccca gacttcttca agcaatcctt cgtaagttta aacatgattt
     1981 tactaactaa ctaatctgat ttaaattttc agcctgaggg cttcacatgg gagagagtca
     2041 ccacatacga agacgggggc gtgcttaccg ttacccagga caccagcctc caggacggct
     2101 gcttgatcta caacgtcaag ctcagagggg tgaacttccc atccaacggc cctgtgatgc
     2161 agaagaaaac actcggctgg gaggccagta ccgagaccct gtaccccgct gacggcggcc
     2221 tggaaggcag atgcgacatg gccctgaagc tcgtgggcgg gggccacctg cactgcaacc
     2281 tgaagaccac atacagatcc aagaaacccg ctaagaacct caagatgcca ggagtaagtt
     2341 taaacatata tatactaact aaccctgatt atttaaattt tcaggtctac tttgtggacc
     2401 gcagactgga aagaatcaag gaggccgaca atgagaccta cgtcgagcag cacgaggtgg
     2461 ctgtggccag atactgcgac ctccctagca aactggggca caaacttaat ggcatggacg
     2521 agctgtacaa gtaagcgccg gtcgctacca ttaccaactt gtctggtgtc aaaaataata
     2581 ggggccgctg tcatcagagt aagtttaaac tgagttctac taactaacga gtaatattta
     2641 aattttcagc atctcgcgcc cgtgcctctg acttctaagt ccaattactc ttcaacatcc
     2701 ctacatgctc tttctccctg tgctcccacc ccctattttt gttattatca aaaaacttct
     2761 cttaatttct ttgtttttta gcttctttta agtcacctct aacaatgaaa ttgtgtagat
     2821 tcaaaaatag aattaattcg taataaaaag tcgaaaaaaa ttgtgctccc tccccccatt
     2881 aataataatt ctatcccaaa atctacacaa tgttctgtgt acacttctta tgttttttac
     2941 ttctgataaa tttttttgaa acatcataga aaaaaccgca cacaaaatac cttatcatat
     3001 gttacgtttc agtttatgac cgcaattttt atttcttcgc acgtctgggc ctctcatgac
     3061 gtcaaatcat gctcatcgtg aaaaagtttt ggagtatttt tggaattttt caatcaagtg
     3121 aaagtttatg aaattaattt tcctgctttt gctttttggg gtttccccta ttgtttgtca
     3181 agatttcgag gacggcgttt ttcttgctaa aatcacaagt attgatgagc acgatgcaag
     3241 aaagatcgga agaaggtttg ggtttgaggc tcagtggaag gtgagtagaa gttgataatt
     3301 tgaaagtgga gtagtgtcta tggggttttt gccttaaatg acagaataca ttcccaatat
     3361 accaaacata actgtttcct actagtcggc cgtacgggcc ctttcgtctc gcgcgtttcg
     3421 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt
     3481 aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc
     3541 ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt
     3601 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcggcct taagggcctc
     3661 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
     3721 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     3781 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     3841 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     3901 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     3961 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     4021 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     4081 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     4141 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     4201 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     4261 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     4321 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     4381 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     4441 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     4501 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     4561 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     4621 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     4681 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     4741 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     4801 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     4861 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     4921 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     4981 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     5041 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     5101 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     5161 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     5221 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     5281 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     5341 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     5401 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     5461 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     5521 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     5581 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgatgaa
     5641 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     5701 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg
     5761 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg
     5821 tgtggaattg tgagcggata acaatt
//
