LOCUS       sequence-376050-        7017 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  cho-1(3.7-1.7k) fluorescent neural reporter driving nuclear CYOFP 
            expression (refer to NeuroPAL paper for expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7017)
  AUTHORS   Yemini E, Lin A, Nejatbakhsh A, Varol E, Sun R, Mena GE, Samuel ADT,
            Paninski L, Venkatachalam V, Hobert O
  TITLE     NeuroPAL: A Multicolor Atlas for Whole-Brain Neuronal Identification
            in C. elegans.
  JOURNAL   Cell. 2021 Jan 7;184(1):272-288.e11. doi: 
            10.1016/j.cell.2020.12.012. Epub 2020 Dec 29.
  PUBMED    33378642
REFERENCE   2  (bases 1 to 7017)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.012"; journalName: "Cell"; date: "2021-01-7-
            7"; volume: "184"; issue: "1"; pages: "272-288.e11"
COMMENT     Sequence Label: pEY73
FEATURES             Location/Qualifiers
     source          1..7017
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6..22
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2376..2396
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2418..2438
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(4608..4630)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4730..4749
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4827..4845)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4913..5017
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5018..5086,5087..5878)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5236..5255)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6049..6637
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6538..6557
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6791..6808
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6925..6946
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6961..6966,6967..6984,6985..6991)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6999..7015
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(7004..7017,1..9)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcctgcagt tcactctttc
      181 cgcttcgaaa agtcgtgtgc ttcttcttct tcttgctccg ccgctgctcc gaattggtta
      241 cggaaacttg aactttcttc gatgttttct tcttcgtcgt agtcttccca gtgacgtcgt
      301 cttcttttct ttttcttatt gcttgtgctg ctcaccacca aaatcattgc aaattcaatt
      361 gagaactcgc ggcggcgatg tctccaagtg ttcgaaacag acaaaaaact aaataaaaga
      421 aaaagaagaa gcatgacatg ttgcctttaa attcttcctc aatttttctt caaacttcca
      481 cccccacagt ggcaaaaaac cacatttgct ccgtggccgc gtgacaaaaa ttgatggctg
      541 agttttcatc tctcgcggcc acgtaggata ctagaaagaa gagtgccctc tagaaaactc
      601 ggccacgggt gcgcggaatt tcaaatttcc tctgggtttt tgaagattcc gagcaacttt
      661 cgttttagtc taggtccgag aaaattcgcg gccgccaatt ttttacggcc atgtaggaaa
      721 aaattcattt taggagcatt gaatgatatt gtaggtccta ccgaatttct aaagcgatta
      781 ccgtacccgt ttaaaggcgc atgccacttt tcaccaccct tcacgaccta taaatttatt
      841 ttcgatagtt gcaaaaaaag cgaagaaaac aagaaacgct tgacaaaaca tttccgcagt
      901 tgcaaatcct ttcttaaaac ttgtttgatt ggctcaatcc gttgctcttt tgctcatcct
      961 cctctctttc ccatacacac acatcgaaat atgaatcttc tcttaaaaag aaggttgtcc
     1021 aattagtttc ccctattcat ttttccatgt tttcttataa caactttttc ttttgaatac
     1081 tcctccagaa atgataaatc tttgtgagat gccaattaaa atttattttt gaattttatt
     1141 tgttgaagga ttttatttgt tgaatgacct aaatagaggc tccgcccata tcttggcttt
     1201 ctgcaatttg cataaaaggt tttccagttt tcgtaggtat tttttgaagg tcttccacgt
     1261 aggcgttgcc ggcgtgccta cgcatacctt ctgcatattg ccaaagtact tctgaatcct
     1321 ttcttccaac tatttgattg aatagagaaa accgaggaac tacactaggt aggcaggcag
     1381 gtcgacaggc agtggctacc ttagctgacc agtgaatttt ttttcaaatc gtgttattga
     1441 gacacttgtg cagaagttta aagtgcacag ttttaaaata ttaggcgtct tgttcaaatt
     1501 tgaattttaa tgcctaaata gataggtagg taggtaggca gcacggtagg caagaagtaa
     1561 gataggtagg aaggtatgtt agtaactatt ttataaacta tatacgttat cgaaaggatc
     1621 taaaattttt ctttggggtt caaaaaaggt cctagttttt tgcaataaaa aaaacacaat
     1681 caacgccaac gctctagcta ggtaggcaag ccggtaggta ggcacgcaga taaattggct
     1741 agtaggtagg taggtaggta ggtgggtagg taggtaggta agtaggtata tagctatagg
     1801 taagtgagta agtaagctgt atttttgtgg ttctgagagc ttactacaat tttttctgaa
     1861 aattcgtatt attttttaaa ataaaaaaag ctatcaaccc tcttctaagg tttcaaaaat
     1921 gaaatcattc tcagcgctcc cttttgtaat cagatatcct tcgaaaaaaa gaataccgtc
     1981 aatcttctta aatttcctca aatttcaagc cttttctgga accccggttc caaatttctg
     2041 acggcttctt acaaaagcat atgcacaaat cttttgctcg taaatcatgt gcattcgaaa
     2101 tgatgaggtg ggccctttcg gggtgagacc ggtctcttag cattccgccc ccaatttttc
     2161 caaacttttt ctgccattca ctcaaatatc cattcaattt tcgtttgaaa ataaactttt
     2221 tttttcattc attcaatttc aatttagcat agaacttcga gcccgggatt ggccaaagga
     2281 cccaaaggta tgtttcgaat gatactaaca taacatagaa cattttcagg aggacccttg
     2341 cttggagggt accgagctca gaaaaaatga ccgctccaaa gaagaagcgc aaggttccag
     2401 tcgagaagat gactgcccca aaaaagaaac gtaaggtccc gcgggtaagt ttaaacagtt
     2461 cggtactaac taaccataca tatttaaatt ttcagcgcgc tgtgtcaaaa ggagaagagc
     2521 ttattaagga gaatatgcgt tccaagctct acctcgaggg atccgtcaac ggacaccaat
     2581 tcaagtgcac ccacgaggga gagggaaagc catacgaggg aaagcaaacc aaccgtatca
     2641 aggtcgtcga gggaggacca ctcccattcg ccttcgacat cctcgccacc cacttcatgt
     2701 acggatccaa ggtaagttta aacatatata tactaactaa ccctgattat ttaaattttc
     2761 aggtcttcat caagtaccca gccgacctcc cagactactt caagcaatcc ttcccagagg
     2821 gattcacctg ggagcgtgtc atggtcttcg aggacggagg agtcctcacc gccacccaag
     2881 acacctccct ccaagacgga gagctcatct acaacgtcaa ggtccgtgga gtcaacttcc
     2941 cagccaacgg accagtcatg caaaagaaga ccctcggatg ggagccatcc accgagacca
     3001 tgtacccagc cgacggagga ctcgagggac gttgcgacaa ggtaagttta aacatgattt
     3061 tactaactaa ctaatctgat ttaaattttc aggccctcaa gctcgtcgga ggaggacacc
     3121 tccacgtcaa cttcaagacc acctacaagt ccaagaagcc agtcaagatg ccaggagtcc
     3181 actacgtcga ccgtcgtctc gagcgtatca aggaggccga caacgagacc tacgtcgagc
     3241 aatacgagca cgccgtcgcc cgttactcca acctcggagg aggaatggac gagctctaca
     3301 agcctaggat accggtgggt gaagacccac caaagccatc tgccaaggga gccaagaagg
     3361 ccgccaagac cgttacgaag ccaaaggacg gaaagaagag acgtcatgcc cgtaaggaat
     3421 catactccgt ctacatctac cgtgtcctca agcaagttca tccagacact ggagtttcct
     3481 ccaaagccat gtctatcatg aactcttttg tcaacgatgt cttcgagcgt attgctgctg
     3541 aagcatcccg tcttgctcac tacaacaagc gttccacaat ctcatcccgc gaaattcaga
     3601 ccgctgtccg tctgatcctt ccaggagagc ttgccaagca cgccgtgtct gagggaacca
     3661 aggccgttac caagtacact tccagcaagt aggaattcca actgagcgcc ggtcgctacc
     3721 attaccaact tgtctggtgt caaaaataat aggggccgct gtcatcagag taagtttaaa
     3781 ctgagttcta ctaactaacg agtaatattt aaattttcag catctcgcgc ccgtgcctct
     3841 gacttctaag tccaattact cttcaacatc cctacatgct ctttctccct gtgctcccac
     3901 cccctatttt tgttattatc aaaaaacttc tcttaatttc tttgtttttt agcttctttt
     3961 aagtcacctc taacaatgaa attgtgtaga ttcaaaaata gaattaattc gtaataaaaa
     4021 gtcgaaaaaa attgtgctcc ctccccccat taataataat tctatcccaa aatctacaca
     4081 atgttctgtg tacacttctt atgtttttta cttctgataa atttttttga aacatcatag
     4141 aaaaaaccgc acacaaaata ccttatcata tgttacgttt cagtttatga ccgcaatttt
     4201 tatttcttcg cacgtctggg cctctcatga cgtcaaatca tgctcatcgt gaaaaagttt
     4261 tggagtattt ttggaatttt tcaatcaagt gaaagtttat gaaattaatt ttcctgcttt
     4321 tgctttttgg ggtttcccct attgtttgtc aagatttcga ggacggcgtt tttcttgcta
     4381 aaatcacaag tattgatgag cacgatgcaa gaaagatcgg aagaaggttt gggtttgagg
     4441 ctcagtggaa ggtgagtaga agttgataat ttgaaagtgg agtagtgtct atggggtttt
     4501 tgccttaaat gacagaatac attcccaata taccaaacat aactgtttcc tactagtcgg
     4561 ccgtacgggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg
     4621 cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt
     4681 cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag
     4741 cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga
     4801 aaataccgca tcaggcggcc ttaagggcct cgtgatacgc ctatttttat aggttaatgt
     4861 catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac
     4921 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     4981 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     5041 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     5101 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     5161 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     5221 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     5281 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     5341 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     5401 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     5461 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     5521 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     5581 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     5641 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     5701 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     5761 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     5821 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     5881 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     5941 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     6001 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     6061 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     6121 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     6181 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     6241 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     6301 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     6361 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     6421 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     6481 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     6541 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     6601 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     6661 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     6721 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     6781 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
     6841 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
     6901 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
     6961 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatt
//
