LOCUS       sequence-376051-        9285 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  acc-2 fluorescent neural reporter driving nuclear mNeptune2.5 
            expression (refer to NeuroPAL paper for expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9285)
  AUTHORS   Yemini E, Lin A, Nejatbakhsh A, Varol E, Sun R, Mena GE, Samuel ADT,
            Paninski L, Venkatachalam V, Hobert O
  TITLE     NeuroPAL: A Multicolor Atlas for Whole-Brain Neuronal Identification
            in C. elegans.
  JOURNAL   Cell. 2021 Jan 7;184(1):272-288.e11. doi: 
            10.1016/j.cell.2020.12.012. Epub 2020 Dec 29.
  PUBMED    33378642
REFERENCE   2  (bases 1 to 9285)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.012"; journalName: "Cell"; date: "2021-01-7-
            7"; volume: "184"; issue: "1"; pages: "272-288.e11"
COMMENT     Sequence Label: pEY60
FEATURES             Location/Qualifiers
     source          1..9285
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6..22
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             4960..4980
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5005..5025
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5047..5067
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(6876..6898)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6998..7017
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7095..7113)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7181..7285
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7286..7354,7355..8146)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7504..7523)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8317..8905
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8806..8825
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    9193..9214
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9229..9234,9235..9252,9253..9259)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9267..9283
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(9272..9285,1..9)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcctgcagc gagaagctgt
      181 acgaaatcta tttagaaaat ggaaaattgt gaaaagtgga tctttgtgaa tttgttaaat
      241 tatagaaatt ttgtactcga atatggtttc aatttttttt tctttatgat ctcagggtaa
      301 tcgggtcatt tgacatcaat ttatacatat gtatatgttt cccttttcta tttaatgaaa
      361 gtattttgaa catcataaag ttactacatt tttatcaaaa atgatttccg aaaaaaacag
      421 tgttcctaat tgttcagaaa atgaaacaat aacttaaaat ttgtcagacc taaaaacatc
      481 tactcgtgag atcaactttg gtccgcataa ttatgggcca ccctgtaggc catttttgca
      541 tgtcttttta attaaatcta tcactgttaa cctgaaatta tgcgggaaca caaatatgta
      601 cgtgtacccg cagtatgtca tctctgtact ataatttctt gatccaccca cgcaattatg
      661 ggctgtttct gccccgaaat gcccattttc catttttttc acttctgtta gtaggaatgt
      721 ccaaatcgac gtaattgtaa gtgacatgct ctcggagcac ccaaaacttt tttttctttt
      781 tcaacgtttt cccaatatat actgggaaaa taaggtgccc ctccggaaat tcatcgaaag
      841 taaacgagag tgagaaaagg aaagagcaac tttgttacca catttttttt ccagttggtc
      901 gaccgacgtg gacaactcta aaaatgtgtt tattgaacac tgaaattgta ttttttattt
      961 tttatatcat gaaaagattt ttgatctttg gaatattgtt tgaactcaat tagagaacaa
     1021 ttaaaatttt cttgaagaaa ataacagttc tagtaaactt tagtaggaat ctatggattt
     1081 tgcggttttt ttttcaaatt acctacaaga aaactactga accgtcctaa aactgattga
     1141 gttatgagct tctttcgttg aatatgacag ctgttagatt tttatctcaa ttgcaccaat
     1201 tagttgacta tttggttttc aaatgtaggg ttttcacaat tttttttgat atacggcgca
     1261 caaatacaaa tttttgcaga gcagtctatt cttaaaatgc taagactatt tggaaaaacg
     1321 aaaaaaatcc atcaatttct aatggtttga atcggattag gaaaggatgc aaagaatgaa
     1381 ggaaaataaa ttccgattct ttatttcttt ttttcgactt tgttgttttt caatttgagt
     1441 atgattttag tatcaaaata taattttaat ttctttagta agcttgcatt aatattcttg
     1501 aatcttatga agttttcgtt ctttcgaata aataaaacat tatgtcaaaa gtagcgggga
     1561 ttttgccaaa acattcataa aatggagcag cacaaaaaat ttaaattaaa cactgaacaa
     1621 ttttttgatc tttcaaaatt ttaaataggt tttggcaaat accactgatt aaagcatcaa
     1681 taaaatgtaa aattcatata aaatattttg gttgcaatcc gaagttctga tagacgaaag
     1741 caatgcaaat gccactattt gaaaagtttt actatgatat tctgtcattt cagtaccatt
     1801 gaagtaactt taatcgttat aaatctgccg acttttcatc tttcaattag ttcaggtttt
     1861 tgagtcaaaa ttttataatg tagaaaacgt gttcatatct tgtttttttt attcttccaa
     1921 aaattgataa ccaacaaaaa aattgcattt attaaattac ttacttcttc atagggaaaa
     1981 ctttatttta acttacaggg tgggccaaaa gtatggtaac acgtttgcag ctctataact
     2041 tcacgtgtgg agagagttat tacatcaatt tgtacaagtt ttgttctcca taaaatactg
     2101 accagatgaa cgttttgaat tttcaaaata ttcccatccg catcgctgca ggccttcaga
     2161 actttttaga tcacgacctt tatggcacgc aagtcggcat tctgcgggat ttctacattt
     2221 tctcgaggaa tccatcaagt gtcttacaag tttttatagt tcaaagaatg ctcttggtct
     2281 agagattctt taaaaacaaa aatccaaata atttctatca cggtattttt gtcatggact
     2341 gcggcagtag gttaagtctt gaatggtagt cgccagaaag atggatgtga gcggcgagta
     2401 gcagtatgca gaaaaattca ttttagtatt tttcgagacg ttttgacctg actttccaga
     2461 atttttttag aaaattgagc tcggcggggc agatagctca gtcggtagtg gtggccgcta
     2521 gcagactgga ggtcacgagt tcaagtccgg cctcaccccc taggttcacc cagcctctat
     2581 tgggaagtgg agcaatccac gactggatta tcggccacag tccccggcta ggacgtggct
     2641 taaattacag cccagaggga tcaccaccag gcagtgtacc tgaatcccag atccgcagtg
     2701 catagcactt gaagaacgga tcgtccttta atccttttta atcctttaat gattttgtgg
     2761 gtcgttacgc catgcgaaac caaaaaaact gagtaaattt cgcaacgttg aattcatgag
     2821 acattcaaag gttcttgcta ggcgccagtg tcgacacaga aaatttttat atgctacaaa
     2881 aacaattaaa aacgcttctt cacttcttta actttaaatt taacataaaa atcatacatc
     2941 tgaaaattat tggcgtgcgt gggaggtgtg tttatgtggg aagtgccgcg tgccaaatta
     3001 ccgttttttc gaaagggttc gccaaattgt cgaggtgata aagatcgcac cttattggga
     3061 aacattacaa attttgagtc aaattgaagg aaatttattc agctatcagt attcgataga
     3121 agaatgtacg tatgacgttg cagtcgcgcg aaaagctcat gtaaaagagc gcttcatgtg
     3181 ttaccatact tttggcccac cctgtaaaaa aaaacaactt caggctcagt tgaataaatt
     3241 gaatgtattt gacgctttgg tgtttagcct attcaagttg ctataccgct accatcgtag
     3301 ttttcctatt tcttgctaaa aatagctttg taaaacaaac caagaataga ttatccagtt
     3361 caaaatgttg aaagtataaa cataaaaatt cggtcctaaa aagtttacat tgtgaaaacg
     3421 tcattctctc atgtaaacat gtctgcacat tccttttctt cgtattcctg cacacaaaca
     3481 aaatatcatt tttctatccg ttcccctcag aaaaaagttc aaaaaattga aaagaggaaa
     3541 gtcccaggag ctcataaaaa gtgctcatgt gtcccacatg ttctttcaaa aagaacattc
     3601 catttggcac catgacacct tccggaagat attcaaatgc ttctcatgaa gataaagctc
     3661 tttcaattct tttgcttaca acagataatt gccctatgtg taattgtgcc gtagaagaat
     3721 atataaaggg cggaatatag gggaaagcat aggcggacgg aggggttgtg ccatctgaaa
     3781 atgcacaaag tgactccttt tttgcccgtc tctcattatt tggatagatg aagaggagga
     3841 cgaaggttga cttctcttcc tgtgaagaag atatactcat caataaatca ttcactcgta
     3901 aaaggatccc gcatgttttt ctttcagaat tttctcattc attctgattt tctatccgcc
     3961 tctgattttt ggttatgtct tttgacatca gtttcgaaag ttccttttta tttccagttt
     4021 ctacttttga atattcattt ttagatagtt ttcctaccgt aaccacgcta aaattgtgtt
     4081 acagtttttt ttcagcttta aaaatgtaaa ttagaaatat tagtttcact atagttgagt
     4141 atttctgttc accgtttttt tgacccactg tatgaaaatg tgactcagtt taatacggaa
     4201 attttttagt cgctcgccac ttcttttaac ttcaatttct ccaccaattt tgatataatt
     4261 tttttcaagc ctcagaatgg tttgaaactg ttgaaatgca tttattatac ataatgtaga
     4321 agaagaataa ttttgccttg tatgctttca aaatatacat ttcctggcga agaaaaatcc
     4381 ctttaaactc cctacaagta tcacacatct caaaactgaa aaatagtcaa attacttttt
     4441 catttaaatt cttcattttt catccattca tcttcaaaat cgagctctca ttctctcact
     4501 tccgctgacc tatattatga tgtgagcatt tatgagattg gacgaacttt attgaaatag
     4561 ccattctttc tgtaactcac aaagttttta tttacttctt tgttttcatt aaaaattgtt
     4621 taagaatgaa ataaaaaatt ttcagacctt ttagagatct tcgttcattt ctcaaagtcg
     4681 ttcctaattt ttctttggat acacaatttc attacgtttc gtacagagtt gacactttga
     4741 cacacgtctt tttttctgca aatgtcagca ttcaaacgaa gaacactgat agatgtttaa
     4801 tcttaaaatt taattttcag acccgggatt ggccaaagga cccaaaggta tgtttcgaat
     4861 gatactaaca taacatagaa cattttcagg aggacccttg gagggtaccg gttttcccag
     4921 tcacgacgcg tggtaccgag ctcagaaaaa atgaccgctc caaagaagaa acgcaaagta
     4981 ccgagctcag aaaaaatgac ggcacctaaa aagaaacgca aagtaccggt cgccgagaag
     5041 acagccccaa aaaagaagcg taaagttcca cgcgtcatgg gagtaagttt aaacagttcg
     5101 gtactaacta accatacata tttaaatttt cagcgcgctg tctccaaggg agaggagctc
     5161 atcaaggaga acatgcacac caagctctac atggagggaa ccgtcaacaa ccaccacttc
     5221 aagtgcaccc acgaagggga gggcaagccc tacgagggca cccagaccaa caggattaag
     5281 gtggtggagg gaggccccct gccgttcgca ttcgacatcc tggccacctg ctttatgtac
     5341 gggagcaaga ccttcatcaa ccacacccaa ggaatcccag acttcttcaa gcaatccttc
     5401 gtaagtttaa acatgatttt actaactaac taatctgatt taaattttca gcctgagggc
     5461 ttcacatggg agagagtcac cacatacgaa gacgggggcg tgcttaccgt tacccaggac
     5521 accagcctcc aggacggctg cttgatctac aacgtcaagc tcagaggggt gaacttccca
     5581 tccaacggcc ctgtgatgca gaagaaaaca ctcggctggg aggccagtac cgagaccctg
     5641 taccccgctg acggcggcct ggaaggcaga tgcgacatgg ccctgaagct cgtgggcggg
     5701 ggccacctgc actgcaacct gaagaccaca tacagatcca agaaacccgc taagaacctc
     5761 aagatgccag gagtaagttt aaacatatat atactaacta accctgatta tttaaatttt
     5821 caggtctact ttgtggaccg cagactggaa agaatcaagg aggccgacaa tgagacctac
     5881 gtcgagcagc acgaggtggc tgtggccaga tactgcgacc tccctagcaa actggggcac
     5941 aaacttaatg gcatggacga gctgtacaag taagcgccgg tcgctaccat taccaacttg
     6001 tctggtgtca aaaataatag gggccgctgt catcagagta agtttaaact gagttctact
     6061 aactaacgag taatatttaa attttcagca tctcgcgccc gtgcctctga cttctaagtc
     6121 caattactct tcaacatccc tacatgctct ttctccctgt gctcccaccc cctatttttg
     6181 ttattatcaa aaaacttctc ttaatttctt tgttttttag cttcttttaa gtcacctcta
     6241 acaatgaaat tgtgtagatt caaaaataga attaattcgt aataaaaagt cgaaaaaaat
     6301 tgtgctccct ccccccatta ataataattc tatcccaaaa tctacacaat gttctgtgta
     6361 cacttcttat gttttttact tctgataaat ttttttgaaa catcatagaa aaaaccgcac
     6421 acaaaatacc ttatcatatg ttacgtttca gtttatgacc gcaattttta tttcttcgca
     6481 cgtctgggcc tctcatgacg tcaaatcatg ctcatcgtga aaaagttttg gagtattttt
     6541 ggaatttttc aatcaagtga aagtttatga aattaatttt cctgcttttg ctttttgggg
     6601 tttcccctat tgtttgtcaa gatttcgagg acggcgtttt tcttgctaaa atcacaagta
     6661 ttgatgagca cgatgcaaga aagatcggaa gaaggtttgg gtttgaggct cagtggaagg
     6721 tgagtagaag ttgataattt gaaagtggag tagtgtctat ggggtttttg ccttaaatga
     6781 cagaatacat tcccaatata ccaaacataa ctgtttccta ctagtcggcc gtacgggccc
     6841 tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag
     6901 acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca
     6961 gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg
     7021 agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc
     7081 aggcggcctt aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat
     7141 ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     7201 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     7261 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     7321 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     7381 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     7441 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     7501 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     7561 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     7621 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     7681 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     7741 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     7801 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     7861 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     7921 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     7981 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     8041 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     8101 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     8161 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     8221 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     8281 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     8341 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     8401 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     8461 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     8521 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     8581 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     8641 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     8701 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     8761 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     8821 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     8881 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     8941 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     9001 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     9061 cgagtcagtg agcgatgaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
     9121 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
     9181 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat
     9241 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatt
//
