LOCUS       sequence-376053-        7289 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  srab-20 fluorescent neural reporter driving nuclear mNeptune2.5 and 
            mTagBFP2 expression (refer to NeuroPAL paper for expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7289)
  AUTHORS   Yemini E, Lin A, Nejatbakhsh A, Varol E, Sun R, Mena GE, Samuel ADT,
            Paninski L, Venkatachalam V, Hobert O
  TITLE     NeuroPAL: A Multicolor Atlas for Whole-Brain Neuronal Identification
            in C. elegans.
  JOURNAL   Cell. 2021 Jan 7;184(1):272-288.e11. doi: 
            10.1016/j.cell.2020.12.012. Epub 2020 Dec 29.
  PUBMED    33378642
REFERENCE   2  (bases 1 to 7289)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.012"; journalName: "Cell"; date: "2021-01-7-
            7"; volume: "184"; issue: "1"; pages: "272-288.e11"
COMMENT     Sequence Label: pEY53
FEATURES             Location/Qualifiers
     source          1..7289
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6..22
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1580..1600
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1625..1645
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1667..1687
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2591..2644
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             2654..2674
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2696..2716
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(4880..4902)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5002..5021
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(5099..5117)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5185..5289
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5290..5358,5359..6150)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5508..5527)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6321..6909
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6810..6829
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7063..7080
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7197..7218
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7233..7238,7239..7256,7257..7263)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7271..7287
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(7276..7289,1..9)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcctgcagg caatgagcaa
      181 agaatccttg aattgcttaa actcattctt aacattcata tcgggctcaa cttttttttg
      241 gcaactcgaa tgtatacagt ctaataaagt gaacactatt aaagaatgtt atacgcttaa
      301 atgtacataa ttcgcatacc acaacacagt ggcccacgta acccataatt ttaaccgaaa
      361 attttggaaa atcctaaata gtttgatttt tattgagaca aacttctact gacattttat
      421 ttttaaaaat ttgaaagaaa aggttgaaaa ataaattttc aagactgcaa aggttgcaat
      481 tatgtattat cagtcctgac tggaaaccag ttgttagaga attttaacaa ttatttttga
      541 agaaatgtgc ctcaaccgag gtgtgcaatt catttgttct ctcacttttg aatacatgtc
      601 tgaatttttc aaaaggagag cgtattagaa gtttatgatt tttataaaga tttttatatt
      661 tttcaaaatg tttaactatt attatttgcc acatttggtg atgtagaaac atgagaactt
      721 acataaaaat aaaaataacc tttatagtgg cagatgtaaa aatgaaaaat tccaaagggt
      781 aagaactaaa ttcgagtttt ctgttctggc caacttgatc tcacccctct ccctgctcct
      841 attttacatt cttctcgtta ttatcacccg ctaaaaagag ctaaatcctt acacgtttgt
      901 gttttagact tttttcaaca agtcctagaa aggtgctaat caccaaatat gcttacgttg
      961 tgttgtgtag attacatttt tgtttctagt aaccacctga aaaaacaatt acttctaaca
     1021 tgaaaaaatg ccgtgatagt tcattagttc aaccacactt caattattgt tcactgcatt
     1081 tcctcacgca gttagccagg ggcagtagtg ggaattaaat tttttgcaga atttgtctaa
     1141 aatctgagat tttgccacgt tttctctttt gggacacagt tttttttcaa agtataagca
     1201 tttcatgcat attttttcgg actttttatt tatttttttt tttttattat ttgcaaaatt
     1261 tggttctgtt tttttgaaac attaacctac cataaaaaaa aacgttttgg taattttttt
     1321 aggatatata tatcataagc aagataccca caaattttaa atttccactt tttaattcta
     1381 cgatatacca caacaacaaa atttttaaaa aattttaaga agcgattgct aattgttcca
     1441 gcccgggatt ggccaaagga cccaaaggta tgtttcgaat gatactaaca taacatagaa
     1501 cattttcagg aggacccttg gagggtaccg gttttcccag tcacgacgcg tggtaccgag
     1561 ctcagaaaaa atgaccgctc caaagaagaa acgcaaagta ccgagctcag aaaaaatgac
     1621 ggcacctaaa aagaaacgca aagtaccggt cgccgagaag acagccccaa aaaagaagcg
     1681 taaagttcca cgcgtcatgg gagtaagttt aaacagttcg gtactaacta accatacata
     1741 tttaaatttt cagcgcgctg tctccaaggg agaggagctc atcaaggaga acatgcacac
     1801 caagctctac atggagggaa ccgtcaacaa ccaccacttc aagtgcaccc acgaagggga
     1861 gggcaagccc tacgagggca cccagaccaa caggattaag gtggtggagg gaggccccct
     1921 gccgttcgca ttcgacatcc tggccacctg ctttatgtac gggagcaaga ccttcatcaa
     1981 ccacacccaa ggaatcccag acttcttcaa gcaatccttc gtaagtttaa acatgatttt
     2041 actaactaac taatctgatt taaattttca gcctgagggc ttcacatggg agagagtcac
     2101 cacatacgaa gacgggggcg tgcttaccgt tacccaggac accagcctcc aggacggctg
     2161 cttgatctac aacgtcaagc tcagaggggt gaacttccca tccaacggcc ctgtgatgca
     2221 gaagaaaaca ctcggctggg aggccagtac cgagaccctg taccccgctg acggcggcct
     2281 ggaaggcaga tgcgacatgg ccctgaagct cgtgggcggg ggccacctgc actgcaacct
     2341 gaagaccaca tacagatcca agaaacccgc taagaacctc aagatgccag gagtaagttt
     2401 aaacatatat atactaacta accctgatta tttaaatttt caggtctact ttgtggaccg
     2461 cagactggaa agaatcaagg aggccgacaa tgagacctac gtcgagcagc acgaggtggc
     2521 tgtggccaga tactgcgacc tccctagcaa actggggcac aaacttaatg gcatggacga
     2581 gctgtacaag gagggacgtg gatccctcct cacctgcgga gacgtcgagg agaacccagg
     2641 accaatgacc gctccaaaga agaagcgcaa ggttccagtc gagaagatga ctgccccaaa
     2701 aaagaaacgt aaggtcccgc ggatgtcaga gcttattaag gagaatatgc atatgaaact
     2761 gtacatggaa ggaaccgtgg acaaccatca ctttaagtgc acttcggagg gggaaggcaa
     2821 accgtacgag ggcacccaga ccatgcgaat caaggtggtc gagggaggcc cactgccctt
     2881 cgccttcgac attctggtaa gtttaaacat atatatacta actaaccctg attatttaaa
     2941 ttttcaggct actagcttcc tgtacggcag caagactttt attaatcaca cccagggcat
     3001 cccggacttc ttcaaacagt cgttccctga gggcttcaca tgggagcgtg tgaccacata
     3061 cgaagacggt ggcgttctca ccgctaccca ggacaccagc ctccaggacg gttgcctcat
     3121 atataatgtg gtaagtttaa acagttcggt actaactaac catacatatt taaattttca
     3181 gaagatccgg ggagtaaact tcacatccaa tggtccggtt atgcagaaga aaacactcgg
     3241 ctgggaggcc ttcaccgaga cgctgtatcc ggctgacgga ggcctggaag gtcgaaacga
     3301 catggccctg aagctcgtcg gcggtagcca tctcatcgca aacgctaaga ccacatatag
     3361 atccaagaaa ccggtaagtt taaacatgat tttactaact aactaatctg atttaaattt
     3421 tcaggctaag aaccttaaaa tgccgggagt ctactatgtt gactacagac tggaacggat
     3481 caaggaggcc aacaacgaga cctacgtcga gcagcacgag gttgcagttg ccagatactg
     3541 cgacctcccg agcaaactgg gtcacaagct taatcctagg ataccggtgg gtgaagaccc
     3601 accaaagcca tctgccaagg gagccaagaa ggccgccaag accgttacga agccaaagga
     3661 cggaaagaag agacgtcatg cccgtaagga atcatactcc gtctacatct accgtgtcct
     3721 caagcaagtt catccagaca ctggagtttc ctccaaagcc atgtctatca tgaactcttt
     3781 tgtcaacgat gtcttcgagc gtattgctgc tgaagcatcc cgtcttgctc actacaacaa
     3841 gcgttccaca atctcatccc gcgaaattca gaccgctgtc cgtctgatcc ttccaggaga
     3901 gcttgccaag cacgccgtgt ctgagggaac caaggccgtt accaagtaca cttccagcaa
     3961 gtaggaattc caactgagcg ccggtcgcta ccattaccaa cttgtctggt gtcaaaaata
     4021 ataggggccg ctgtcatcag agtaagttta aactgagttc tactaactaa cgagtaatat
     4081 ttaaattttc agcatctcgc gcccgtgcct ctgacttcta agtccaatta ctcttcaaca
     4141 tccctacatg ctctttctcc ctgtgctccc accccctatt tttgttatta tcaaaaaact
     4201 tctcttaatt tctttgtttt ttagcttctt ttaagtcacc tctaacaatg aaattgtgta
     4261 gattcaaaaa tagaattaat tcgtaataaa aagtcgaaaa aaattgtgct ccctcccccc
     4321 attaataata attctatccc aaaatctaca caatgttctg tgtacacttc ttatgttttt
     4381 tacttctgat aaattttttt gaaacatcat agaaaaaacc gcacacaaaa taccttatca
     4441 tatgttacgt ttcagtttat gaccgcaatt tttatttctt cgcacgtctg ggcctctcat
     4501 gacgtcaaat catgctcatc gtgaaaaagt tttggagtat ttttggaatt tttcaatcaa
     4561 gtgaaagttt atgaaattaa ttttcctgct tttgcttttt ggggtttccc ctattgtttg
     4621 tcaagatttc gaggacggcg tttttcttgc taaaatcaca agtattgatg agcacgatgc
     4681 aagaaagatc ggaagaaggt ttgggtttga ggctcagtgg aaggtgagta gaagttgata
     4741 atttgaaagt ggagtagtgt ctatggggtt tttgccttaa atgacagaat acattcccaa
     4801 tataccaaac ataactgttt cctactagtc ggccgtacgg gccctttcgt ctcgcgcgtt
     4861 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc
     4921 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt
     4981 gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc
     5041 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgg ccttaagggc
     5101 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
     5161 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     5221 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     5281 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     5341 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     5401 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     5461 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     5521 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     5581 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     5641 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     5701 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     5761 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     5821 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     5881 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     5941 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     6001 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     6061 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     6121 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     6181 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     6241 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     6301 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     6361 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     6421 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     6481 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     6541 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     6601 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     6661 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     6721 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     6781 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     6841 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     6901 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     6961 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     7021 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     7081 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     7141 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
     7201 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg
     7261 ttgtgtggaa ttgtgagcgg ataacaatt
//
