LOCUS       sequence-376056-        5508 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  aqp-6 fluorescent neural reporter driving nuclear mNeptune2.5 
            expression (refer to NeuroPAL paper for expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5508)
  AUTHORS   Yemini E, Lin A, Nejatbakhsh A, Varol E, Sun R, Mena GE, Samuel ADT,
            Paninski L, Venkatachalam V, Hobert O
  TITLE     NeuroPAL: A Multicolor Atlas for Whole-Brain Neuronal Identification
            in C. elegans.
  JOURNAL   Cell. 2021 Jan 7;184(1):272-288.e11. doi: 
            10.1016/j.cell.2020.12.012. Epub 2020 Dec 29.
  PUBMED    33378642
REFERENCE   2  (bases 1 to 5508)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.012"; journalName: "Cell"; date: "2021-01-7-
            7"; volume: "184"; issue: "1"; pages: "272-288.e11"
COMMENT     Sequence Label: pEY64
FEATURES             Location/Qualifiers
     source          1..5508
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6..22
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1183..1203
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1228..1248
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1270..1290
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(3099..3121)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3221..3240
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(3318..3336)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3404..3508
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3509..3577,3578..4369)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3727..3746)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4540..5128
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5029..5048
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    5416..5437
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5452..5457,5458..5475,5476..5482)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5490..5506
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(5495..5508,1..9)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcctgcagt cagacacatt
      181 tggcgtttct atacggaaaa ctacaagttt acatagctgc acctatggcg acttatattt
      241 gttttgattt tttggtttcg gacttcaaat aatattactt ttgttgatca caaatactat
      301 tcttcaactt tcaaaaaacc gtccacaatt ttttaaaatt aaattttcat gtcagcttta
      361 acaacactct ttttcagttc tatttgaccc ttatttgacc tttgctttta tcaattttta
      421 taatcatatt tgcaaataaa atgattgcca aggcaaatat tttgtctgaa aaatattaaa
      481 agtcaacaaa gttatgaaac tttttctacc gaaaactatc aaaaagtctg agactgagac
      541 acggctatat ttttatttgg ctctcacgat tctggaaaag tacttttgta tgctgactac
      601 tgtactttct cacatacaaa gatatatatt tacatttctc gacatcattc ctttttttat
      661 tctgtaagca ttttccagaa agcttctgat agtactcatc aaaacttgaa agatggtcat
      721 aaaagtcaac agcgggggtc tttatggcgc cgatgtattt gaactgaaaa cattcggaaa
      781 atatcgagga gaaatgaaca ggaaacccga ctttgagcag gtaacgtttt ttatattttc
      841 ataatgtatt acagtggtca cttttctata attttctcta tgtacagttc atgacaaaaa
      901 atcaggtgct ggaggtgaaa acaagtcgat tactctcaat ctaatttatc tccgttcctc
      961 cttacattta cccgaaaata gtttctatag gtcattggga aaaacattac atttattttt
     1021 tcaaagttct attcttattt caaacccggg attggccaaa ggacccaaag gtatgtttcg
     1081 aatgatacta acataacata gaacattttc aggaggaccc ttggagggta ccggttttcc
     1141 cagtcacgac gcgtggtacc gagctcagaa aaaatgaccg ctccaaagaa gaaacgcaaa
     1201 gtaccgagct cagaaaaaat gacggcacct aaaaagaaac gcaaagtacc ggtcgccgag
     1261 aagacagccc caaaaaagaa gcgtaaagtt ccacgcgtca tgggagtaag tttaaacagt
     1321 tcggtactaa ctaaccatac atatttaaat tttcagcgcg ctgtctccaa gggagaggag
     1381 ctcatcaagg agaacatgca caccaagctc tacatggagg gaaccgtcaa caaccaccac
     1441 ttcaagtgca cccacgaagg ggagggcaag ccctacgagg gcacccagac caacaggatt
     1501 aaggtggtgg agggaggccc cctgccgttc gcattcgaca tcctggccac ctgctttatg
     1561 tacgggagca agaccttcat caaccacacc caaggaatcc cagacttctt caagcaatcc
     1621 ttcgtaagtt taaacatgat tttactaact aactaatctg atttaaattt tcagcctgag
     1681 ggcttcacat gggagagagt caccacatac gaagacgggg gcgtgcttac cgttacccag
     1741 gacaccagcc tccaggacgg ctgcttgatc tacaacgtca agctcagagg ggtgaacttc
     1801 ccatccaacg gccctgtgat gcagaagaaa acactcggct gggaggccag taccgagacc
     1861 ctgtaccccg ctgacggcgg cctggaaggc agatgcgaca tggccctgaa gctcgtgggc
     1921 gggggccacc tgcactgcaa cctgaagacc acatacagat ccaagaaacc cgctaagaac
     1981 ctcaagatgc caggagtaag tttaaacata tatatactaa ctaaccctga ttatttaaat
     2041 tttcaggtct actttgtgga ccgcagactg gaaagaatca aggaggccga caatgagacc
     2101 tacgtcgagc agcacgaggt ggctgtggcc agatactgcg acctccctag caaactgggg
     2161 cacaaactta atggcatgga cgagctgtac aagtaagcgc cggtcgctac cattaccaac
     2221 ttgtctggtg tcaaaaataa taggggccgc tgtcatcaga gtaagtttaa actgagttct
     2281 actaactaac gagtaatatt taaattttca gcatctcgcg cccgtgcctc tgacttctaa
     2341 gtccaattac tcttcaacat ccctacatgc tctttctccc tgtgctccca ccccctattt
     2401 ttgttattat caaaaaactt ctcttaattt ctttgttttt tagcttcttt taagtcacct
     2461 ctaacaatga aattgtgtag attcaaaaat agaattaatt cgtaataaaa agtcgaaaaa
     2521 aattgtgctc cctcccccca ttaataataa ttctatccca aaatctacac aatgttctgt
     2581 gtacacttct tatgtttttt acttctgata aatttttttg aaacatcata gaaaaaaccg
     2641 cacacaaaat accttatcat atgttacgtt tcagtttatg accgcaattt ttatttcttc
     2701 gcacgtctgg gcctctcatg acgtcaaatc atgctcatcg tgaaaaagtt ttggagtatt
     2761 tttggaattt ttcaatcaag tgaaagttta tgaaattaat tttcctgctt ttgctttttg
     2821 gggtttcccc tattgtttgt caagatttcg aggacggcgt ttttcttgct aaaatcacaa
     2881 gtattgatga gcacgatgca agaaagatcg gaagaaggtt tgggtttgag gctcagtgga
     2941 aggtgagtag aagttgataa tttgaaagtg gagtagtgtc tatggggttt ttgccttaaa
     3001 tgacagaata cattcccaat ataccaaaca taactgtttc ctactagtcg gccgtacggg
     3061 ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg
     3121 gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg
     3181 tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta
     3241 ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc
     3301 atcaggcggc cttaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
     3361 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     3421 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     3481 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     3541 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     3601 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     3661 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     3721 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     3781 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     3841 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     3901 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     3961 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     4021 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     4081 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     4141 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     4201 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     4261 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     4321 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     4381 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     4441 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     4501 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     4561 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     4621 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     4681 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     4741 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     4801 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     4861 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     4921 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     4981 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     5041 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     5101 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     5161 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     5221 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     5281 cagcgagtca gtgagcgatg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc
     5341 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
     5401 tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt
     5461 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatt
//
