LOCUS       sequence-376058-        7980 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ceh-9 fluorescent neural reporter driving nuclear mTagBFP2 
            expression (refer to NeuroPAL paper for expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7980)
  AUTHORS   Yemini E, Lin A, Nejatbakhsh A, Varol E, Sun R, Mena GE, Samuel ADT,
            Paninski L, Venkatachalam V, Hobert O
  TITLE     NeuroPAL: A Multicolor Atlas for Whole-Brain Neuronal Identification
            in C. elegans.
  JOURNAL   Cell. 2021 Jan 7;184(1):272-288.e11. doi: 
            10.1016/j.cell.2020.12.012. Epub 2020 Dec 29.
  PUBMED    33378642
REFERENCE   2  (bases 1 to 7980)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.012"; journalName: "Cell"; date: "2021-01-7-
            7"; volume: "184"; issue: "1"; pages: "272-288.e11"
COMMENT     Sequence Label: pEY71
FEATURES             Location/Qualifiers
     source          1..7980
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6..22
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3345..3365
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3387..3407
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(5571..5593)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5693..5712
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(5790..5808)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5876..5980
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5981..6049,6050..6841)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6199..6218)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7012..7600
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7501..7520
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7754..7771
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7888..7909
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7924..7929,7930..7947,7948..7954)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7962..7978
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(7967..7980,1..9)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcctgcagc aactccgact
      181 agatcgcttt tgaaaaatct gggaatttcc ataatttcaa aatttgggaa attttagaga
      241 aggcgatttt ctaatagcgg taatttttcg agatacattt ttttcttgat ttttttttgg
      301 aatttttatt tcctgaactt tggaagtttt cggccacaag agtgttttat attttgaaaa
      361 ctctgtaatt tttgcgcatc ttacagtaat tttaaatttt ttgtaatttt gaaaaatttg
      421 acttttgaaa atcgttaatt ttggttataa agaaattaac aaagaaattt tatagtattt
      481 ggctttttaa aaattatatt tttgaaagtt tagccatttt tagaatttta aaaattaaga
      541 aaaattaaaa aattaaactg aattttcata gaagagtttt ctaacacata ggccaggtag
      601 agaaagccaa aacatgccat ttttcctccc aatttccact ttttcatata ccttcaaaca
      661 attatagcca aaatgagcac tttgagctgg tgccaagtgc caggcgtgag aatatcacaa
      721 gtgtataatc aaagcggaga actctttatt tttcaaatgt ttccttggaa gtatataata
      781 ctcatacggg tgtgtgtgtt ttaatgttca gcattttagg tattgataaa ataagttcaa
      841 gctagaaatt ttccttgttt tcccaaaaaa aaatttttaa ggtggggtac cgaaatctgg
      901 gaaatatttt tgaatgaatc ctaattttcc cctgattccg agtatctatg tgaaaaaatt
      961 caaaattcaa aacaagtttg ccagatttta tatttaagct tgaaatcgcc gaatttcatt
     1021 tgtatcccgg tctctttttt aaatacgcgc tcaaataaat tagcattgga gcgcgcttgc
     1081 attgtttgat tttttttcgt ttttttacat ttttttcaga aaaaaaaaac cgacaaaaat
     1141 gaatgaaact ggttttaaaa aaattgaatt cggctaaaat aaaaaaaact aaacgaaaaa
     1201 aagtcaaacg atgcacgcgc gcgctaattt atttgggcgc gagtttgaaa aaagtgatgc
     1261 gagtacaaat gaaatttaaa aaaaaaaaat ttttatgaaa gatttcaagc ttaaatataa
     1321 attcaggaaa ttttttttga attttttcac atagatattc ggaatcaggg gaaaatttag
     1381 ggtgaattaa aaatatttcc aagattcaag tttttttggt tagaccctta ttaatttttg
     1441 cgtgctgacg tcacaatttt tctggaaaaa tattcccgca tttttcgtag atcaaaccgc
     1501 aatgagacag cctgacacca cgtgtatcgg tttattttta agataccaca tggaaattga
     1561 caaattctcg attcaccatg actaaaacta gaatattaat aaaattttag ctataagtat
     1621 ttgtctgttg cttctcgaca aagctgtaga aaaagtttcg aaaaattgaa tttttgaatt
     1681 tgaatttttg atttttttga tcccaagttt ttttccagtt cgttccagta ataattatat
     1741 atatatctag gcaaataaat ctcccctcgc ttgaaataaa aaaaaataaa ctcaagacat
     1801 atcaaataag caatcaactt tcatgtccgc tatataaggt ctgaaacatt tatggaaaaa
     1861 gtttgtttgc cacaaagatt tattcgttga cattttttaa gaatgttccc aagatggaag
     1921 tcatgaattt atactttctt ggaataattt tattaccaac tgttttctga tttttttttg
     1981 gttaaaaatg tccccccgtc tcatttcatt ttcccgctag aatatatttt ttctcctgca
     2041 acctataaac ttccgataat tgatgatatt taaataaaag tgttcagtga tattacggaa
     2101 aaaatgcaaa aacctcaaac tttccaaaat aacgtacgac tttttaaaag taagtaattt
     2161 acatttaaca tgcgtagtat tatgtttctc tagttttaca ttttcctcaa gttctaatag
     2221 gaatatatca aacttcaaaa gaaatttaga gcatttggga gcaatttggg ctcggaaaat
     2281 ttttttggtt tttttattgt atttttaaat taaaaaaaaa agagccgtga aattgctcaa
     2341 aaaaaaaaag gtttttttgc gtgcgggttg catatcctca ttttaaaagt aattttttag
     2401 gctataaaaa tataaacttg tgaaagatat gtttttttct ggattttgtg aatttctgga
     2461 aatttctctt tctttttttt ctacccttcc ctcacccccg cctgagctta ttctgaagcc
     2521 aacacctaag cctaagccta agcctaagcg taagcctaag cctaagtctg agcataagcc
     2581 taagcctcag cctaagcctc agcctaagcc taagcctaag cctaagccta ggcctaaatt
     2641 taagtgtagc cttaagtcta gacctaagcc taagcctaac aataagctta tactcagtag
     2701 acatccttac aaaattaatt cgatttccat gaaaaattgt agatcaacct tgctggtcta
     2761 ttcaaaaggt ttttttcaaa taattgaggt tatatttgaa agtccaagta ttacaaatta
     2821 gcacaataat atcacatgtg cctccgctac tccaaaattt caattcaaat atttaagttc
     2881 aagaaaagta tttaatagtc aagtatgaga aactcattcc ctcaattgca tcaagtatca
     2941 tcccttacca ccaatcctcc tctccccctc ctccttctcg aaatccggtc tcttattcaa
     3001 tttgctggct catgctcttt ttgtcatcat ctcagaactc acccatcttc ttcttctttc
     3061 cacaccaatt gcgcaatcat tgtcgaccac tttggcaaat tgtcgtcgtc gtcaaccgtc
     3121 aaaagtcccc cgagagtcca gaatttcaat tatttgattg aagagaaggc ggggacctcg
     3181 agacctactg tatagagaag tctctcagaa gttcttttca ttcgtgcaaa cccgggattg
     3241 gccaaaggac ccaaaggtat gtttcgaatg atactaacat aacatagaac attttcagga
     3301 ggacccttgc ttggagggta ccgagctcag aaaaaatgac cgctccaaag aagaagcgca
     3361 aggttccagt cgagaagatg actgccccaa aaaagaaacg taaggtcccg cggatgtcag
     3421 agcttattaa ggagaatatg catatgaaac tgtacatgga aggaaccgtg gacaaccatc
     3481 actttaagtg cacttcggag ggggaaggca aaccgtacga gggcacccag accatgcgaa
     3541 tcaaggtggt cgagggaggc ccactgccct tcgccttcga cattctggta agtttaaaca
     3601 tatatatact aactaaccct gattatttaa attttcaggc tactagcttc ctgtacggca
     3661 gcaagacttt tattaatcac acccagggca tcccggactt cttcaaacag tcgttccctg
     3721 agggcttcac atgggagcgt gtgaccacat acgaagacgg tggcgttctc accgctaccc
     3781 aggacaccag cctccaggac ggttgcctca tatataatgt ggtaagttta aacagttcgg
     3841 tactaactaa ccatacatat ttaaattttc agaagatccg gggagtaaac ttcacatcca
     3901 atggtccggt tatgcagaag aaaacactcg gctgggaggc cttcaccgag acgctgtatc
     3961 cggctgacgg aggcctggaa ggtcgaaacg acatggccct gaagctcgtc ggcggtagcc
     4021 atctcatcgc aaacgctaag accacatata gatccaagaa accggtaagt ttaaacatga
     4081 ttttactaac taactaatct gatttaaatt ttcaggctaa gaaccttaaa atgccgggag
     4141 tctactatgt tgactacaga ctggaacgga tcaaggaggc caacaacgag acctacgtcg
     4201 agcagcacga ggttgcagtt gccagatact gcgacctccc gagcaaactg ggtcacaagc
     4261 ttaatcctag gataccggtg ggtgaagacc caccaaagcc atctgccaag ggagccaaga
     4321 aggccgccaa gaccgttacg aagccaaagg acggaaagaa gagacgtcat gcccgtaagg
     4381 aatcatactc cgtctacatc taccgtgtcc tcaagcaagt tcatccagac actggagttt
     4441 cctccaaagc catgtctatc atgaactctt ttgtcaacga tgtcttcgag cgtattgctg
     4501 ctgaagcatc ccgtcttgct cactacaaca agcgttccac aatctcatcc cgcgaaattc
     4561 agaccgctgt ccgtctgatc cttccaggag agcttgccaa gcacgccgtg tctgagggaa
     4621 ccaaggccgt taccaagtac acttccagca agtaggaatt ccaactgagc gccggtcgct
     4681 accattacca acttgtctgg tgtcaaaaat aataggggcc gctgtcatca gagtaagttt
     4741 aaactgagtt ctactaacta acgagtaata tttaaatttt cagcatctcg cgcccgtgcc
     4801 tctgacttct aagtccaatt actcttcaac atccctacat gctctttctc cctgtgctcc
     4861 caccccctat ttttgttatt atcaaaaaac ttctcttaat ttctttgttt tttagcttct
     4921 tttaagtcac ctctaacaat gaaattgtgt agattcaaaa atagaattaa ttcgtaataa
     4981 aaagtcgaaa aaaattgtgc tccctccccc cattaataat aattctatcc caaaatctac
     5041 acaatgttct gtgtacactt cttatgtttt ttacttctga taaatttttt tgaaacatca
     5101 tagaaaaaac cgcacacaaa ataccttatc atatgttacg tttcagttta tgaccgcaat
     5161 ttttatttct tcgcacgtct gggcctctca tgacgtcaaa tcatgctcat cgtgaaaaag
     5221 ttttggagta tttttggaat ttttcaatca agtgaaagtt tatgaaatta attttcctgc
     5281 ttttgctttt tggggtttcc cctattgttt gtcaagattt cgaggacggc gtttttcttg
     5341 ctaaaatcac aagtattgat gagcacgatg caagaaagat cggaagaagg tttgggtttg
     5401 aggctcagtg gaaggtgagt agaagttgat aatttgaaag tggagtagtg tctatggggt
     5461 ttttgcctta aatgacagaa tacattccca atataccaaa cataactgtt tcctactagt
     5521 cggccgtacg ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
     5581 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
     5641 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca
     5701 gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg
     5761 agaaaatacc gcatcaggcg gccttaaggg cctcgtgata cgcctatttt tataggttaa
     5821 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
     5881 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     5941 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     6001 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     6061 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     6121 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     6181 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     6241 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     6301 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     6361 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     6421 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     6481 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     6541 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     6601 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     6661 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     6721 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     6781 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     6841 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     6901 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     6961 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     7021 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     7081 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     7141 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     7201 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     7261 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     7321 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     7381 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     7441 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     7501 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     7561 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     7621 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     7681 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     7741 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
     7801 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
     7861 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
     7921 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
//
