LOCUS       sequence-376059-        9271 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For use in RBP tethering screen.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9271)
  AUTHORS   Luo EC, Nathanson JL, Tan FE, Schwartz JL, Schmok JC, Shankar A, 
            Markmiller S, Yee BA, Sathe S, Pratt GA, Scaletta DB, Ha Y, Hill DE,
            Aigner S, Yeo GW
  TITLE     Large-scale tethered function assays identify factors that regulate 
            mRNA stability and translation.
  JOURNAL   Nat Struct Mol Biol. 2020 Oct;27(10):989-1000. doi: 
            10.1038/s41594-020-0477-6. Epub 2020 Aug 17.
  PUBMED    32807991
REFERENCE   2  (bases 1 to 9271)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41594-020-0477-6"; journalName: "Nat Struct Mol Biol"; 
            date: "2020-10"; volume: "27"; issue: "10"; pages: "989-1000"
COMMENT     Sequence Label: DDX11
FEATURES             Location/Qualifiers
     source          1..9271
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..1234
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          287..1225
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1182..1202
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     1251..1270
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1251..1269
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1348..1372
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    2020..2031
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     protein_bind    complement(4283..4307)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4432..4473
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(4928..4945)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4934..5158
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5291..5310)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    5442..5489
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             5497..6519
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             5497..6519
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    6649..6770
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6686..6705)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6740..6759
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6819..6835)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6819..6835)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6832..6854)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6843..6859
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6867..6873,6874..6891,6892..6897))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6912..6933
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7050..7067)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7221..7809)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7301..7320)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7980..8771,8772..8840))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8603..8622
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8841..8945)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9020..9039)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 agagaggaat ctttgcagct aatggacctt ctaggtcttg aaaggagtgg gaattggctc
       61 cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg
      121 ggtcggcaat tgaaccggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt
      181 cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt
      241 cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg
      301 tggttcccgc gggcctggcc tctttacggg ttatggccct tgcgtgcctt gaattacttc
      361 cacctggctg cagtacgtga ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag
      421 ttcgaggcct tgcgcttaag gagccccttc gcctcgtgct tgagttgagg cctggcctgg
      481 gcgctggggc cgccgcgtgc gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga
      541 taagtctcta gccatttaaa atttttgatg acctgctgcg acgctttttt tctggcaaga
      601 tagtcttgta aatgcgggcc aagatctgca cactggtatt tcggtttttg gggccgcggg
      661 cggcgacggg gcccgtgcgt cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg
      721 gccaccgaga atcggacggg ggtagtctca agctggccgg cctgctctgg tgcctggcct
      781 cgcgccgccg tgtatcgccc cgccctgggc ggcaaggctg gcccggtcgg caccagttgc
      841 gtgagcggaa agatggccgc ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg
      901 gcgctcggga gagcgggcgg gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc
      961 agccgtcgct tcatgtgact ccacggagta ccgggcgccg tccaggcacc tcgattagtt
     1021 ctcgagcttt tggagtacgt cgtctttagg ttggggggag gggttttatg cgatggagtt
     1081 tccccacact gagtgggtgg agactgaagt taggccagct tggcacttga tgtaattctc
     1141 cttggaattt gccctttttg agtttggatc ttggttcatt ctcaagcctc agacagtggt
     1201 tcaaagtttt tttcttccat ttcaggtgtc gtgaggaatt agcttggtac taatacgact
     1261 cactataggg agacccaagc tggctaggta agcttggtac cgagctcgga tccactagtc
     1321 cagtgtggtg gaattctgca gatatcaaca agtttgtaca aaaaagttgg catggctaat
     1381 gaaacacaga aggttggtgc catccatttt ccttttccct tcacacccta ttccatccag
     1441 gaagacttca tggcagagct gtaccgggtt ttggaggctg gcaagattgg gatatttgag
     1501 agtccaactg gcactgggaa gtccttaagt cttatttgtg gggccctctc ttggctccgt
     1561 gactttgaac agaagaagcg tgaagaagag gcacgactcc ttgaaactgg aactggcccc
     1621 ttacatgatg agaaagatga atccctgtgt ctgtcttctt cctgcgaagg ggctgcaggc
     1681 accccgaggc ctgctggaga accggcctgg gttactcagt ttgtgcagaa gaaagaagag
     1741 agggacctgg tggaccgact aaaggcggag caggccagga ggaagcagcg agaagaacgc
     1801 ctgcagcagc tgcagcacag ggtgcagctc aagtatgcag ccaagcgcct gaggcaggaa
     1861 gaagaagaaa gagagaatct cctccgcctc agcagggaga tgctagagac aggcccggag
     1921 gctgagcggc tggagcagct ggagtctggg gaggaggagc tggtcctcgc cgaatacgag
     1981 agtgatgagg agaaaaaggt ggcgagcaga gtggatgagg atgaggatga cctggaggaa
     2041 gaacacataa ctaagattta ttactgtagt cggacacact cccagctggc ccagtttgtg
     2101 catgaggtga agaagagccc ctttggcaag gatgttcggc tggtctccct tggctcccgg
     2161 cagaaccttt gtgtaaatga agacgtgaaa agcctaggtt ctgtgcagct tatcaacgac
     2221 cgctgtgtgg acatgcagag aagcaggcac gagaagaaga aaggagctga ggaggagaag
     2281 ccaaagagga ggaggcagga gaagcaggca gcctgcccct tctacaacca cgagcagatg
     2341 ggccttctcc gggatgaggc cctggcagag gtgaaggaca tggagcagct gctggccctt
     2401 gggaaggagg cccgggcctg tccctattac gggagccgcc ttgccatccc tgcagcccag
     2461 ctggtggtgc tgccctatca gatgctgctg catgcggcca ctcggcaggc cgcgggcatc
     2521 cggctgcagg accaggtggt gatcatcgac gaggcgcaca acctgatcga caccatcacg
     2581 ggcatgcaca gcgtggaggt cagcggctcc cagctctgcc aggcccattc ccagctgctg
     2641 cagtacgtgg agcgatacgg gaagcgtttg aaggccaaga acctgatgta cctgaagcag
     2701 atcctgtatt tgctggagaa attcgtggct gtgctagggg ggaacattaa gcaaaatccc
     2761 aatacacaga gtctgtcaca gacagggacg gagctgaaga ccatcaacga ctttctcttc
     2821 cagagccaga tcgacaacat caacctgttc aaggtgcagc gatactgtga gaagagcatg
     2881 atcagcagaa agctctttgg attcactgaa cggtacggag cagtgttctc atcccgggag
     2941 cagcccaaac tggctgggtt tcagcaattc ctgcagagcc tgcagcccag gacgactgaa
     3001 gctcttgcag cccctgcaga cgagagtcag gccagcaccc tgcgaccagc ttctccactg
     3061 atgcacatcc aaggcttcct ggcagctctc actacggcca accaggacgg cagggtcatc
     3121 ctgagccgcc aaggcagcct cagtcagagc accctgaagt ttttgctcct gaatccagct
     3181 gtgcactttg cccaagtggt gaaggaatgc cgggcagtgg tcattgcggg gggtaccatg
     3241 cagccggtgt ctgacttccg gcagcagctg ctggcctgtg ccggggtgga agctgagcgc
     3301 gtggtggagt tttcctgtgg tcacgtgatc cctccagaca acatcctgcc cctcgtcatc
     3361 tgcagcggga tctccaacca gccgctggaa ttcacgttcc agaaaagaga gctgcctcag
     3421 atgatggacg aggtgggtcg cattctctgt aacctgtgcg gtgtggttcc tggaggggtg
     3481 gtctgtttct tcccctccta cgagtacctg cgccaggtcc atgcccactg ggagaagggt
     3541 ggcctgctgg gccgtctggc tgccaggaag aagatattcc aggaacctaa gagcgcacac
     3601 caggtggagc aggtgctgct ggcatattcc aggtgcatcc aggcctgtgg ccaggagaga
     3661 ggccaggtga caggggccct gctcctctct gtggttggag gaaagatgag tgaagggatc
     3721 aacttctctg acaacctagg ccggtgtgtg gtgatggtgg gcatgccctt ccccaacatc
     3781 aggtctgcag agctgcagga gaagatggcc tacttggatc aaaccctcag cccccggcca
     3841 ggcaccccca gggaaggctc tggtggagaa cctgtgcatg aaggccgtca accagtccat
     3901 aggcagggcc atcaggcacc agaaggattt tgccagcgta gtgctcctgg accagcgata
     3961 tgcccggccc cctgtcctgg ccaagctgcc ggcctggatc cgagcccgtg tggaggtcaa
     4021 agctaccttt ggccccgcca ttgctgctgt gcagaagttt caccgggaga agtcggcctc
     4081 ttcctgatgg gcaaccacac cactgcctgg cgccgtgccc ttcctttgtc ctgcccgctg
     4141 gagacagtgt ttgtcgtggg cgtggtctgc ggggatcctg ttacaaaggt gaaacccagg
     4201 aggagagtgt ggagtccaga gtgctgccag gacccaggca caggcgttag ctcccgtagg
     4261 agaaaatggg ggaatcctga atgcccaact ttcttgtaca aagtggttga tatccagcac
     4321 agtggcggcc gctcgagtct agagggcccg cggttcgaaa ccagcggagg cggcggaagc
     4381 gggggaggag gcagcggcgg aggcggcagc gggggcggcg gaagcgctac cggtaagcct
     4441 atccctaacc ctctcctcgg tctcgattct acgcgtaccg gtatggcttc taactttact
     4501 cagttcgttc tcgtcgacaa tggcggaact ggcgacgtga ctgtcgcccc aagcaacttc
     4561 gctaacgggg tcgctgaatg gatcagctct aactcgcgat cacaggctta caaagtaacc
     4621 tgtagcgttc gtcagagctc tgcgcagaat cgcaaataca ccatcaaagt cgaggtgcct
     4681 aaagtggcaa cccagactgt tggtggtgaa gagcttcctg tagccggatg gagatcttac
     4741 ttaaatatgg aactaaccat tccaattttc gccacgaatt ccgactgcga gcttattgtt
     4801 aaggcaatgc aaggtctcct aaaagatgga aacccgattc cctcggccat cgcggccaac
     4861 tccggcatct acggaggtgg aggtggatct cgtacgggtt agtaatgagt ttaaacccgc
     4921 tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg
     4981 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt
     5041 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc
     5101 aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct
     5161 tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc
     5221 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     5281 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     5341 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     5401 gaccccaaaa aacttgatta gggtgatggt tcacgtacct agaagttcct attccgaagt
     5461 tcctattctc tagaaagtat aggaacttcc ttggccaaaa agcctgaact caccgcgacg
     5521 tctgtcgaga agtttctgat cgaaaagttc gacagcgtct ccgacctgat gcagctctcg
     5581 gagggcgaag aatctcgtgc tttcagcttc gatgtaggag ggcgtggata tgtcctgcgg
     5641 gtaaatagct gcgccgatgg tttctacaaa gatcgttatg tttatcggca ctttgcatcg
     5701 gccgcgctcc cgattccgga agtgcttgac attggggaat tcagcgagag cctgacctat
     5761 tgcatctccc gccgtgcaca gggtgtcacg ttgcaagacc tgcctgaaac cgaactgccc
     5821 gctgttctgc agccggtcgc ggaggccatg gatgcgatcg ctgcggccga tcttagccag
     5881 acgagcgggt tcggcccatt cggaccgcaa ggaatcggtc aatacactac atggcgtgat
     5941 ttcatatgcg cgattgctga tccccatgtg tatcactggc aaactgtgat ggacgacacc
     6001 gtcagtgcgt ccgtcgcgca ggctctcgat gagctgatgc tttgggccga ggactgcccc
     6061 gaagtccggc acctcgtgca cgcggatttc ggctccaaca atgtcctgac ggacaatggc
     6121 cgcataacag cggtcattga ctggagcgag gcgatgttcg gggattccca atacgaggtc
     6181 gccaacatct tcttctggag gccgtggttg gcttgtatgg agcagcagac gcgctacttc
     6241 gagcggaggc atccggagct tgcaggatcg ccgcggctcc gggcgtatat gctccgcatt
     6301 ggtcttgacc aactctatca gagcttggtt gacggcaatt tcgatgatgc agcttgggcg
     6361 cagggtcgat gcgacgcaat cgtccgatcc ggagccggga ctgtcgggcg tacacaaatc
     6421 gcccgcagaa gcgcggccgt ctggaccgat ggctgtgtag aagtactcgc cgatagtgga
     6481 aaccgacgcc ccagcactcg tccgagggca aaggaatagc acgtactacg agatttcgat
     6541 tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg
     6601 atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt
     6661 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     6721 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt
     6781 ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga
     6841 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     6901 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     6961 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     7021 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     7081 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     7141 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     7201 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     7261 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     7321 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     7381 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     7441 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     7501 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     7561 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     7621 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     7681 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     7741 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     7801 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     7861 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     7921 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     7981 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     8041 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     8101 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     8161 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     8221 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     8281 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     8341 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     8401 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     8461 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     8521 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     8581 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     8641 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     8701 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     8761 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     8821 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     8881 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     8941 ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tctcccgatc
     9001 ccctatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatctgc
     9061 tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa
     9121 ggcaaggctt gaccgacaat tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc
     9181 ttcgcgatgt acgggccaga tatacgcgtt gacattgatt attgactagg cttttgcaaa
     9241 aagctttgca aagatggata aagttttaaa c
//
