LOCUS       sequence-376060-        6413 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  aqp-5 fluorescent neural reporter driving nuclear mNeptune2.5 
            expression (refer to NeuroPAL paper for expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6413)
  AUTHORS   Yemini E, Lin A, Nejatbakhsh A, Varol E, Sun R, Mena GE, Samuel ADT,
            Paninski L, Venkatachalam V, Hobert O
  TITLE     NeuroPAL: A Multicolor Atlas for Whole-Brain Neuronal Identification
            in C. elegans.
  JOURNAL   Cell. 2021 Jan 7;184(1):272-288.e11. doi: 
            10.1016/j.cell.2020.12.012. Epub 2020 Dec 29.
  PUBMED    33378642
REFERENCE   2  (bases 1 to 6413)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.012"; journalName: "Cell"; date: "2021-01-7-
            7"; volume: "184"; issue: "1"; pages: "272-288.e11"
COMMENT     Sequence Label: pEY63
FEATURES             Location/Qualifiers
     source          1..6413
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6..22
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2088..2108
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2133..2153
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2175..2195
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(4004..4026)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4126..4145
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4223..4241)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4309..4413
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4414..4482,4483..5274)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4632..4651)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5445..6033
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5934..5953
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    6321..6342
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6357..6362,6363..6380,6381..6387)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6395..6411
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(6400..6413,1..9)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcctgcagt ttcggtctgc
      181 tccagtgagc gcagctcgac gatgaaagct gtgcatcttt tgaaatactc aactcacatt
      241 ccggatttgt gaagacattc cttaaatgtt taaaatatga tgaagaaaag gaaaaatgta
      301 ggttgaagtg gggaaaaaag caagatgctt ctacgataat tcaacagaat tcagagaaaa
      361 tggtgaaaga tttattgatt ttatgattga ttgttgttgg ttgaagcttt aacaaattgt
      421 agtaaagcgt gtaaagagaa gaagatggat tgaactagat cttatttgtt aactgaatga
      481 agagtttgaa ctctttccag acagcttcat catgctccta acacgtgtgg tgaaatattt
      541 cagaacaatt ttttttgagg gaagaatatt gatataagtc atggaaaagg aagaagagtt
      601 ttaagaagga ggtggataaa aagaagaaga aataggtgga cacgattgat atgatatgaa
      661 tattgcattt gaaaatgtgg ccacgacagg atgtgagcaa gcttgttgag gagaccgcag
      721 agccaaccgg atgacttctc gtctctcaac tgatatcttt ttttcgactc acccatttga
      781 agacgttgaa atcggctgaa aacttggtgg aattttaagg aaaacgtgtg agtcgagaaa
      841 actgccaaaa actctgagca ggagttatga acagcaagaa agattctgga aataaccaat
      901 gattctgaat aagcgggagc attgttagct catatccgag ctttcagatt gtttgaattt
      961 cagtttttaa caagccatgt tttctctaat ttgcattgta aacaatatca ctttcaattt
     1021 gatcatttta ttgaaattct aatgggttac tgtgtaaaat acggtaggtg tgtaaaaata
     1081 acacaaaagt attaggttat ccagcatcaa taaaaaatta tatttgcatt gaaactaggc
     1141 cgcataagca ttcatgctcg tttttttgaa tttcaaattt ctagacattt ttgaatcagg
     1201 atataaatgt tgaatatcta ttctgatgat gatacatttt taagctacta ctccaagcag
     1261 ttagagtgtt ttaatatctt gtgcagcttt aaatttattt taattttttt attggtagca
     1321 tcttgatccc acgcacattt acacattcat atataacaag atattctcac cgcttccgga
     1381 atcaaaattt cggaaattgc agttttaatg ttgatatgac attaatctta gaactcagat
     1441 cgtaagattt catgtttttt ttttcttcga ttagtgcatt attcaaatcg agttcaccaa
     1501 ttgaattaca attatctttg gcttcagaca tttctccgca tcgactattg tttttggcaa
     1561 aaaagctttc cttaaaacag agaaaatatg tatttctcaa tattgatatt ttgaactttt
     1621 tgcattcatt ccccaatttc aaacatccat ttatttttcc actcaaaatt ctttcaaatg
     1681 ttctttgttt tttccgtcgt taacagtatc atgttaagtc atgtttccgg tttttgaact
     1741 ctttttttct aggtattact ttacataatt tttcactgca gttatgaaaa aagaagcaaa
     1801 aagcaaaaat cctacaaaat cgctcaggtt acaggaaccc ttactttgtc ttgtaactta
     1861 acactcatca tcccgaacca aataaagtag tcaaaggact tttcatttct acttattcaa
     1921 cccctctctc ataattcttc ttttgaactc ccgggattgg ccaaaggacc caaaggtatg
     1981 tttcgaatga tactaacata acatagaaca ttttcaggag gacccttgga gggtaccggt
     2041 tttcccagtc acgacgcgtg gtaccgagct cagaaaaaat gaccgctcca aagaagaaac
     2101 gcaaagtacc gagctcagaa aaaatgacgg cacctaaaaa gaaacgcaaa gtaccggtcg
     2161 ccgagaagac agccccaaaa aagaagcgta aagttccacg cgtcatggga gtaagtttaa
     2221 acagttcggt actaactaac catacatatt taaattttca gcgcgctgtc tccaagggag
     2281 aggagctcat caaggagaac atgcacacca agctctacat ggagggaacc gtcaacaacc
     2341 accacttcaa gtgcacccac gaaggggagg gcaagcccta cgagggcacc cagaccaaca
     2401 ggattaaggt ggtggaggga ggccccctgc cgttcgcatt cgacatcctg gccacctgct
     2461 ttatgtacgg gagcaagacc ttcatcaacc acacccaagg aatcccagac ttcttcaagc
     2521 aatccttcgt aagtttaaac atgattttac taactaacta atctgattta aattttcagc
     2581 ctgagggctt cacatgggag agagtcacca catacgaaga cgggggcgtg cttaccgtta
     2641 cccaggacac cagcctccag gacggctgct tgatctacaa cgtcaagctc agaggggtga
     2701 acttcccatc caacggccct gtgatgcaga agaaaacact cggctgggag gccagtaccg
     2761 agaccctgta ccccgctgac ggcggcctgg aaggcagatg cgacatggcc ctgaagctcg
     2821 tgggcggggg ccacctgcac tgcaacctga agaccacata cagatccaag aaacccgcta
     2881 agaacctcaa gatgccagga gtaagtttaa acatatatat actaactaac cctgattatt
     2941 taaattttca ggtctacttt gtggaccgca gactggaaag aatcaaggag gccgacaatg
     3001 agacctacgt cgagcagcac gaggtggctg tggccagata ctgcgacctc cctagcaaac
     3061 tggggcacaa acttaatggc atggacgagc tgtacaagta agcgccggtc gctaccatta
     3121 ccaacttgtc tggtgtcaaa aataataggg gccgctgtca tcagagtaag tttaaactga
     3181 gttctactaa ctaacgagta atatttaaat tttcagcatc tcgcgcccgt gcctctgact
     3241 tctaagtcca attactcttc aacatcccta catgctcttt ctccctgtgc tcccaccccc
     3301 tatttttgtt attatcaaaa aacttctctt aatttctttg ttttttagct tcttttaagt
     3361 cacctctaac aatgaaattg tgtagattca aaaatagaat taattcgtaa taaaaagtcg
     3421 aaaaaaattg tgctccctcc ccccattaat aataattcta tcccaaaatc tacacaatgt
     3481 tctgtgtaca cttcttatgt tttttacttc tgataaattt ttttgaaaca tcatagaaaa
     3541 aaccgcacac aaaatacctt atcatatgtt acgtttcagt ttatgaccgc aatttttatt
     3601 tcttcgcacg tctgggcctc tcatgacgtc aaatcatgct catcgtgaaa aagttttgga
     3661 gtatttttgg aatttttcaa tcaagtgaaa gtttatgaaa ttaattttcc tgcttttgct
     3721 ttttggggtt tcccctattg tttgtcaaga tttcgaggac ggcgtttttc ttgctaaaat
     3781 cacaagtatt gatgagcacg atgcaagaaa gatcggaaga aggtttgggt ttgaggctca
     3841 gtggaaggtg agtagaagtt gataatttga aagtggagta gtgtctatgg ggtttttgcc
     3901 ttaaatgaca gaatacattc ccaatatacc aaacataact gtttcctact agtcggccgt
     3961 acgggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc
     4021 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg
     4081 gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga
     4141 ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat
     4201 accgcatcag gcggccttaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
     4261 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     4321 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     4381 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     4441 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     4501 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     4561 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     4621 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     4681 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     4741 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     4801 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     4861 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     4921 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     4981 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     5041 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     5101 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     5161 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     5221 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     5281 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     5341 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     5401 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     5461 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     5521 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     5581 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     5641 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     5701 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     5761 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     5821 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     5881 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     5941 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     6001 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     6061 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     6121 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     6181 gagcgcagcg agtcagtgag cgatgaagcg gaagagcgcc caatacgcaa accgcctctc
     6241 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
     6301 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
     6361 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca att
//
