LOCUS       sequence-376061-        9070 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  acr-5 fluorescent neural reporter driving nuclear mTagBFP2 
            expression (refer to NeuroPAL paper for expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9070)
  AUTHORS   Yemini E, Lin A, Nejatbakhsh A, Varol E, Sun R, Mena GE, Samuel ADT,
            Paninski L, Venkatachalam V, Hobert O
  TITLE     NeuroPAL: A Multicolor Atlas for Whole-Brain Neuronal Identification
            in C. elegans.
  JOURNAL   Cell. 2021 Jan 7;184(1):272-288.e11. doi: 
            10.1016/j.cell.2020.12.012. Epub 2020 Dec 29.
  PUBMED    33378642
REFERENCE   2  (bases 1 to 9070)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.012"; journalName: "Cell"; date: "2021-01-7-
            7"; volume: "184"; issue: "1"; pages: "272-288.e11"
COMMENT     Sequence Label: pEY14
FEATURES             Location/Qualifiers
     source          1..9070
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6..22
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             4435..4455
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4477..4497
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(6661..6683)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6783..6802
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6880..6898)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6966..7070
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7071..7139,7140..7931)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7289..7308)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8102..8690
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8591..8610
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8844..8861
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8978..8999
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9014..9019,9020..9037,9038..9044)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9052..9068
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(9057..9070,1..9)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcactagtg atctgctcta
      181 ataatggctg gttcagcaca tcaaaacgag tacgactttt cgcaacgacg gcattcactt
      241 atcaacatgt tcaaggcgtt taaattgtaa acaatcttgt gatgcagcat tgctccaatg
      301 ataatttggg tatttcatag ggtgttattt tgcatctgtt tatctatgaa gaagctgaaa
      361 actggaatta tcattagagc aagttcgcaa gcagaccagc acacaaaata atgcaacaga
      421 atttggttcc caaagagctc aataaagttt acttttcaca aatgcttcaa tttccgtttc
      481 ttccatttaa attgttctga tgttatagct tctcgcactt tccctccatt tcaatttccc
      541 gcctattttc taccctcaaa agttcctctc atagttttct tattgatgag gaacttttgg
      601 gcattagcaa aattgtgccc tttgaattaa gttaaaatga attaaataat cttttattaa
      661 agcgaattcg catcatattt taaatgcgcg gtattaataa aaataggttc gtcgatcaat
      721 aggtgttttc atattatcca aatacacaat tgggcaattt gtaaaacttg aagaatgcgg
      781 caacgagttc tgtgcaagtc tttaccttta atgcttccgt catcgttagc aaaaactgtg
      841 tcatgaaaga caagtgtata agattattca aatacaattt tcttatggag tttttctagc
      901 ttttaaggat ttccaaatag aatgatcatt ccttgaataa taattctcta ttttatcaat
      961 attttgtttt tttttctatt ttagaaaggg cggagagtag tgtgtagtgg cggtttcacg
     1021 ctctgatgat ctcatgccat tgttcacctt ttccaaattg ttttaggttt ttcggagtac
     1081 ttcttttctt tcttttccct cttcattttc accctctgtt tgagcatgag tgctctttat
     1141 tctttggtga tggaataaaa agaggtgcca tcaaattcag aattttgtaa aatgttgaac
     1201 tttagagttt ttagtattcg gttagtgtga tgtttgtttt attttcaacc ctaacaaatc
     1261 tcagttttct ggaaaagttg tgcatttaca atgaatatat tgttttctgc atttcgtctg
     1321 ttcttagatt attcagtgac tttggaaatt taagctaatt cttgttgagc catttgacat
     1381 aatttgtata gaaaacaaaa atgtatactt tacaccgcga tttagatacg gcactgtgcg
     1441 gaaactagca aacaattcag agccgacttc cataaggttg ccaaaaaaat tttagtaatt
     1501 tatttaattc aataattaaa tgtatttatc acgaaaataa aatgaaaaca tttgttgttc
     1561 aaaattatat attttcagaa agaaaaaaca agtttttaaa atttaattac tacttctcat
     1621 tatgagtctc tcaaaaagct caacatttca gttagaataa aaattaaaaa atatcaatga
     1681 aagtgtcccg tctaattcca tttttcactc cccacactgt tcaacgtcaa aacacattgt
     1741 cattcagtca ttttggaaaa agcaccccga gcgcagaagc agtaggaaaa acgagtctcg
     1801 atgggggtag gttgaacgag caaaaaaaac caattcggct aattgaactc gactttttct
     1861 cccaatttta ctctcaccaa tcgtgatgcg tatctaaatg gatacgcaaa gaaaggaaaa
     1921 gaaagagctt tttattttca gatccaccca cggacacgtt tttggtagaa ctattacatc
     1981 actttttctt agttttttct gtgagcttga tgagaactgt ggtgtatttt ttgtagataa
     2041 aacaaaattt aaacctactt cataagttca cacacctacc ttatgtcagc ctagaaagtt
     2101 accacgatga tggcagaaag ttgcctcttt gcctctaaat gccgagtagt ttcaaaaaat
     2161 gttgacattt gatttggaat ttaagagtcc tctctgaaaa tgtgtaattc tgaaatttta
     2221 gtgcccattt caaaaaaata acatctgctc agcaaattgt atttcatcct ctctttattt
     2281 tttgttaccc cgtcttactt cctctctttt ttatttattg ttttttcttg tggtcctaat
     2341 aataaaaatg aaaagagaag ctgttgtgct cttccacaac aatgtgctct tttcaatatt
     2401 ctaaaatggc aatggaattg gcaattgttg gagggttaaa attgccagac aaaagtcatc
     2461 ttctctatgt ttgttcttca tcttctttta cctctattcc ttatccttct caataacatt
     2521 ctgaaactga cgatttaatg agtatacttc cgtttatgaa tgtatcatga taatttcatg
     2581 tctgaaaaag aacggagaaa tgagaatgca tattctcaac ttgaacgtat agccaagact
     2641 tatgcctatt ttcaatctgt ttagaccctg acaaacattt gaccacacag gagtgtttgg
     2701 gacaacaacc atatttttga aaacattttt gaatgagaaa tgctatcttc tgtgggaagt
     2761 tttaaagcaa gctgatttct ttatatagcc caaaataatt attttttaat agattatgca
     2821 aattaaaata tggagcttga tttgctgaag tgtctatttt gatttgattt gatttgattt
     2881 gatttattac gattggaatc gtgatggaat aaaagcagta tagtataaag acagaaaaaa
     2941 taaatacaca atacatagga agaataatca aaaagttacg aataaagtac aaacaagaaa
     3001 agcaattatc acctgcaaaa gttgattaat tggaacatta aagtgaacat gaaataggct
     3061 atttgggggt gtgaggagag tgggtgaatg atgggtaata agaggtgcca atataagatt
     3121 cgtcatgcta atttttcata gaactttaga ccctatttgt gaaaaagtgt cttcttagtt
     3181 tggttttgca tttcccccag atgaacttgg ttttggttct ggttttgttg tttttatcga
     3241 ttttgaaaaa ttggtccgtg tcaatgtcaa ctttatttaa ctttaagctt taaagaataa
     3301 acttatcaaa ataaatcaaa cggtactttt tttttgaaat ttttattcaa aattcaatct
     3361 tttcaagagc aaactgtaat tttttttgtc attcctatcg caaataaacg taaaattcag
     3421 ttaatttaaa atgtgagaaa tgaggtaaca actactatag aaaaataatg aaatgttcaa
     3481 gcgatgagca attaaaattt acgctcaaaa attaaccatg atgagaaaaa aattctgctc
     3541 ttcaagttat acaagactta tgagtgttaa ctttatactt caaaaaataa attgtattga
     3601 aacacctttt ttagagattt tagattatta gattgagaac tgtacaaaaa aacgagtgtt
     3661 ccaagataca tgagaatgat attttaaccc gaaacaatga aacattcaat catacatatt
     3721 ctcatccttt tcaactcgtc aacattccac gccttgtctc acttttttct atttcccgtc
     3781 cactgggatt ttgatttccc gcccgtttcg tctcggcatt ttttttttga aagaggttac
     3841 tctctcatct ttgacaccat cttccataaa acacgagaaa tgaataatgg aatcgaggga
     3901 ggttcttgtt tgtttttttt tgtgtgtttt tgttttgcat tttactcata ttttagaact
     3961 tcgaaatctg atcatgactc gttttgcgag aaccattgct ttaaaatatt tttattttct
     4021 tgaggataac tgtttttttg aaaaaaaaaa catggaatct ttggaagtat gctgtaaatt
     4081 gaggaagtct taaaatgttt caacttccat atgaaaaatt aaatataata cagttttgtt
     4141 tccgttacaa aatccaagac gtcttctcac aaaaaatcgc ctaaattttc atttaaaaat
     4201 gtgtctttgc aaaagttacc agtcctgtca aatactcgca acaaatatcc aagaataact
     4261 gactgatctg atttttgcag ttagtttttc ctcttcaaac tcaaaatctg tttttatttg
     4321 aaccattcaa tagtttaaac cccatttgaa tacgttttct catctttcct cgtcaccagt
     4381 ttcaatgctt taaaaacaat tttcagcatg ccgagctcag aaaaaatgac cgctccaaag
     4441 aagaagcgca aggttccagt cgagaagatg actgccccaa aaaagaaacg taaggtcccg
     4501 cggatgtcag agcttattaa ggagaatatg catatgaaac tgtacatgga aggaaccgtg
     4561 gacaaccatc actttaagtg cacttcggag ggggaaggca aaccgtacga gggcacccag
     4621 accatgcgaa tcaaggtggt cgagggaggc ccactgccct tcgccttcga cattctggta
     4681 agtttaaaca tatatatact aactaaccct gattatttaa attttcaggc tactagcttc
     4741 ctgtacggca gcaagacttt tattaatcac acccagggca tcccggactt cttcaaacag
     4801 tcgttccctg agggcttcac atgggagcgt gtgaccacat acgaagacgg tggcgttctc
     4861 accgctaccc aggacaccag cctccaggac ggttgcctca tatataatgt ggtaagttta
     4921 aacagttcgg tactaactaa ccatacatat ttaaattttc agaagatccg gggagtaaac
     4981 ttcacatcca atggtccggt tatgcagaag aaaacactcg gctgggaggc cttcaccgag
     5041 acgctgtatc cggctgacgg aggcctggaa ggtcgaaacg acatggccct gaagctcgtc
     5101 ggcggtagcc atctcatcgc aaacgctaag accacatata gatccaagaa accggtaagt
     5161 ttaaacatga ttttactaac taactaatct gatttaaatt ttcaggctaa gaaccttaaa
     5221 atgccgggag tctactatgt tgactacaga ctggaacgga tcaaggaggc caacaacgag
     5281 acctacgtcg agcagcacga ggttgcagtt gccagatact gcgacctccc gagcaaactg
     5341 ggtcacaagc ttaatcctag gataccggtg ggtgaagacc caccaaagcc atctgccaag
     5401 ggagccaaga aggccgccaa gaccgttacg aagccaaagg acggaaagaa gagacgtcat
     5461 gcccgtaagg aatcatactc cgtctacatc taccgtgtcc tcaagcaagt tcatccagac
     5521 actggagttt cctccaaagc catgtctatc atgaactctt ttgtcaacga tgtcttcgag
     5581 cgtattgctg ctgaagcatc ccgtcttgct cactacaaca agcgttccac aatctcatcc
     5641 cgcgaaattc agaccgctgt ccgtctgatc cttccaggag agcttgccaa gcacgccgtg
     5701 tctgagggaa ccaaggccgt taccaagtac acttccagca agtaggaatt ccaactgagc
     5761 gccggtcgct accattacca acttgtctgg tgtcaaaaat aataggggcc gctgtcatca
     5821 gagtaagttt aaactgagtt ctactaacta acgagtaata tttaaatttt cagcatctcg
     5881 cgcccgtgcc tctgacttct aagtccaatt actcttcaac atccctacat gctctttctc
     5941 cctgtgctcc caccccctat ttttgttatt atcaaaaaac ttctcttaat ttctttgttt
     6001 tttagcttct tttaagtcac ctctaacaat gaaattgtgt agattcaaaa atagaattaa
     6061 ttcgtaataa aaagtcgaaa aaaattgtgc tccctccccc cattaataat aattctatcc
     6121 caaaatctac acaatgttct gtgtacactt cttatgtttt ttacttctga taaatttttt
     6181 tgaaacatca tagaaaaaac cgcacacaaa ataccttatc atatgttacg tttcagttta
     6241 tgaccgcaat ttttatttct tcgcacgtct gggcctctca tgacgtcaaa tcatgctcat
     6301 cgtgaaaaag ttttggagta tttttggaat ttttcaatca agtgaaagtt tatgaaatta
     6361 attttcctgc ttttgctttt tggggtttcc cctattgttt gtcaagattt cgaggacggc
     6421 gtttttcttg ctaaaatcac aagtattgat gagcacgatg caagaaagat cggaagaagg
     6481 tttgggtttg aggctcagtg gaaggtgagt agaagttgat aatttgaaag tggagtagtg
     6541 tctatggggt ttttgcctta aatgacagaa tacattccca atataccaaa cataactgtt
     6601 tcctactagt cggccgtacg ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa
     6661 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag
     6721 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta
     6781 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag
     6841 atgcgtaagg agaaaatacc gcatcaggcg gccttaaggg cctcgtgata cgcctatttt
     6901 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
     6961 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     7021 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     7081 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     7141 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     7201 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     7261 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     7321 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     7381 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     7441 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     7501 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     7561 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     7621 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     7681 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     7741 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     7801 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     7861 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     7921 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     7981 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     8041 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     8101 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     8161 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     8221 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     8281 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     8341 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     8401 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     8461 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     8521 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     8581 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     8641 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     8701 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     8761 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     8821 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
     8881 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
     8941 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
     9001 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
     9061 gataacaatt
//
