LOCUS       sequence-376063-        7632 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  flp-12 fluorescent neural reporter driving nuclear T-Sapphire 
            expression (refer to NeuroPAL paper for expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7632)
  AUTHORS   Yemini E, Lin A, Nejatbakhsh A, Varol E, Sun R, Mena GE, Samuel ADT,
            Paninski L, Venkatachalam V, Hobert O
  TITLE     NeuroPAL: A Multicolor Atlas for Whole-Brain Neuronal Identification
            in C. elegans.
  JOURNAL   Cell. 2021 Jan 7;184(1):272-288.e11. doi: 
            10.1016/j.cell.2020.12.012. Epub 2020 Dec 29.
  PUBMED    33378642
REFERENCE   2  (bases 1 to 7632)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.012"; journalName: "Cell"; date: "2021-01-7-
            7"; volume: "184"; issue: "1"; pages: "272-288.e11"
COMMENT     Sequence Label: pEY79
FEATURES             Location/Qualifiers
     source          1..7632
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6..22
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2922..2942
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2967..2987
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3009..3029
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(5223..5245)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5345..5364
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(5442..5460)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5528..5632
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5633..5701,5702..6493)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5851..5870)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6664..7252
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7153..7172
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7406..7423
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7540..7561
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7576..7581,7582..7599,7600..7606)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7614..7630
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(7619..7632,1..9)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcctgcagc cataacgagt
      181 cggaactaca ttcgtaatga tacttttttt ctaaagcatc actccccaat ggctcgtttc
      241 aatgacgttt ttcaacaaaa tcgaaaacca ccctcgtact ttttcttctg tacaaaaggg
      301 cagtacgatt ctacgctgaa gtgtgaaatg tgtgatgggt atgggaaata gtattgaaaa
      361 aaactatgta actcaaacat tcaaaataca aaagtatcct tcaaaaacag ttgatttcat
      421 tttcaatgtt tcaaaatgac gttagttcta caaaaaaagt taactcaact cgacatgtgc
      481 tactcagcgg ttaaacgtta caacttttca aaatcactac gaaaaacgct aattcgtttt
      541 gcttgcacgt atggaagatt tcagagcaat cataaattat ggataacact gaaagtcttt
      601 aacaacaaaa gtatttttga cggaaatttt taaaaaacgg gaattagatt gacatagtaa
      661 caaaatgtgt ttatcaatta caattacatc aaaaataaaa gtatcatgcg acaaagacat
      721 tcttgattaa atgtcggaat tatcaatgcc gagagtgtgg atcaggccaa ttaaaccgga
      781 ttactcattt cagagtgcta aaatttttga agattagaat caaattcact tcattacgat
      841 gattgctctg aactagctgg gaagagaaac aaggaatatt tttggttttt ttctggaagg
      901 ggattgtttc actgtataat ttttttgatt gtagacaata ttcacccaaa tgctcacttt
      961 tccattttat actattgatt tcagtgtaat ggaatgtacg actgataaaa tcgttccaat
     1021 aaacacttaa gtcaccccac ttgagccaac atttgatatt tgaccaatcc acatactgaa
     1081 acttgagtaa atggttccac ttcctcttcc ttttttcttc aagcccttct gaaaaaagtt
     1141 cattggtttt gaacttgtct taaaacagaa cggaaactta tacgagtttc ctgaacgagg
     1201 aaaagttttg gagcagtttt ttcgaacttt aaagcttttc caaatctgat aaattcaaaa
     1261 gtgttcaaac tggtaaaaag ccagacgttt attaatgtga agacattaaa attgattatg
     1321 aacaaaaaaa gtaatttcga gttgaagtaa atttacaata ggagcatata ttctttttga
     1381 aactgttacc agtattattt ggaaaatctt ttaatgctca ccgtgagacc agctctaata
     1441 ttttgaagct aaaaattcac aacgccacgc ttgcatatct tgctcaaaag tccggtggta
     1501 tcttcagatg gctccttaga aaatcttttg atcatgcagt cttccagcct ggcggccctt
     1561 ttaagcctta tatacgatag ggggattacg gttaatgcta aatggttgca aatgtgtgtt
     1621 ttatttacgt gatgatgttt gatgaatcag catatgaaaa tattttggag aaaaataatg
     1681 agataatcga gttacatgac acctagtttg gagttgataa tattagtatg tttgttgata
     1741 atattactat ttggtttcaa attgaaaaat actgaaactc aaaaattttg gtatacttct
     1801 agaattgcct tcctggaatt ggaaaacata attattgcag agatcaaaat agaagcctga
     1861 gataattctg gatggcttta aagtttcaaa aaccagtagt taaactttga ttcttattgt
     1921 gccattgaaa cgatcttgtt tttaatcaag atgctttggg gagtacttca tctctatgac
     1981 ttatgaattc aaagaaatac acacagtggg cgtcctggac ataagttaat tttggaagag
     2041 tgtacataga ataagaattt tgtttcaaat taatttgaca agtcatcgaa atagtatttt
     2101 acacatttat gggtaggttt tcttgtgtac tttgctgttt tcttgtataa aacagcaaca
     2161 gcgatttcaa aatctgtgaa tagatgcaat tggtggttgc actatataga gagggatagg
     2221 cttctcaaaa tagttccgtt tcgactctgt aaatttgtca tgcttccgga gattgcactc
     2281 caatttagac attctttcaa taatgttttg tacggaatct caaaagtgtc ttgcattgtt
     2341 atattgtgca caatatttct ggacccccac ggtcgcgcct tttgccttcc aatttgcgcc
     2401 ttttgtgcgt gtcacaccag catcactttt tcgccgcctt catccgggat tcctctatcc
     2461 ttcacaatca gtatggagga gaagaagcac tgccggcgga aagcgatgac gagttgtgtg
     2521 gtgagaggac ggattgccca attttccaac tattgtctac catcaatctt ctcccgaaaa
     2581 gacacatgat ttcatttttt cttttccact ccttctattt ttttgctgtc ttggtgacat
     2641 tgagagctca acttcatcca ccataatttc tcctcccaaa aggggaggac catcattagg
     2701 ccacgcccac ttggtcggca cataagctcg ccagattcag atcggcataa tatttgcgcc
     2761 acacaagctg aactttcagt aaacccggga ttggccaaag gacccaaagg tatgtttcga
     2821 atgatactaa cataacatag aacattttca ggaggaccct tggagggtac cggttttccc
     2881 agtcacgacg cgtggtaccg agctcagaaa aaatgaccgc tccaaagaag aaacgcaaag
     2941 taccgagctc agaaaaaatg acggcaccta aaaagaaacg caaagtaccg gtcgccgaga
     3001 agacagcccc aaaaaagaag cgtaaagttc cacgcgtcat gggagtaagt ttaaacagtt
     3061 cggtactaac taaccataca tatttaaatt ttcagcgcgc tgtaagtaag ggagaggagt
     3121 tgttcactgg tgtggtgcca atccttgtcg agcttgatgg agatgttaac ggacacaagt
     3181 tctctgtttc tggagaggga gagggagatg ctacctacgg aaagcttact cttaagttca
     3241 tctgcactac tggaaagctt ccagttccat ggccaaccct tgttaccacc ttctcttacg
     3301 gagttatggt tttcgctcgc tacccagatc atatgaagca acatgatttc ttcaagtctg
     3361 ctatgccaga aggatacgtt caagagcgca ctatcttctt caaggtaagt ttaaacatat
     3421 atatactaac taaccctgat tatttaaatt ttcaggatga tggaaactac aagacccgcg
     3481 ctgaggttaa gttcgaggga gatacccttg ttaaccgcat cgagcttaag ggaatcgact
     3541 tcaaggaaga tggaaacatc cttggacata agcttgaata caacttcaac tctcataacg
     3601 tctacatcat ggctgataag cagaaggtaa gtttaaacat gattttacta actaactaat
     3661 ctgatttaaa ttttcagaac ggaattaagg ctaacttcaa gatccgccat aacatcgagg
     3721 atggaggagt tcaacttgct gatcattacc agcaaaacac tccaatcgga gatggaccag
     3781 ttcttcttcc agataaccac tacctttcta tccaatctaa gctttctaag gatccaaacg
     3841 agaagcgcga tcacatggtc cttcttgagt tcgtcaccgc tgctggaatc acccttggaa
     3901 tggatgagct ctacaagcct aggataccgg tgggtgaaga cccaccaaag ccatctgcca
     3961 agggagccaa gaaggccgcc aagaccgtta cgaagccaaa ggacggaaag aagagacgtc
     4021 atgcccgtaa ggaatcatac tccgtctaca tctaccgtgt cctcaagcaa gttcatccag
     4081 acactggagt ttcctccaaa gccatgtcta tcatgaactc ttttgtcaac gatgtcttcg
     4141 agcgtattgc tgctgaagca tcccgtcttg ctcactacaa caagcgttcc acaatctcat
     4201 cccgcgaaat tcagaccgct gtccgtctga tccttccagg agagcttgcc aagcacgccg
     4261 tgtctgaggg aaccaaggcc gttaccaagt acacttccag caagtaggaa ttccaactga
     4321 gcgccggtcg ctaccattac caacttgtct ggtgtcaaaa ataatagggg ccgctgtcat
     4381 cagagtaagt ttaaactgag ttctactaac taacgagtaa tatttaaatt ttcagcatct
     4441 cgcgcccgtg cctctgactt ctaagtccaa ttactcttca acatccctac atgctctttc
     4501 tccctgtgct cccaccccct atttttgtta ttatcaaaaa acttctctta atttctttgt
     4561 tttttagctt cttttaagtc acctctaaca atgaaattgt gtagattcaa aaatagaatt
     4621 aattcgtaat aaaaagtcga aaaaaattgt gctccctccc cccattaata ataattctat
     4681 cccaaaatct acacaatgtt ctgtgtacac ttcttatgtt ttttacttct gataaatttt
     4741 tttgaaacat catagaaaaa accgcacaca aaatacctta tcatatgtta cgtttcagtt
     4801 tatgaccgca atttttattt cttcgcacgt ctgggcctct catgacgtca aatcatgctc
     4861 atcgtgaaaa agttttggag tatttttgga atttttcaat caagtgaaag tttatgaaat
     4921 taattttcct gcttttgctt tttggggttt cccctattgt ttgtcaagat ttcgaggacg
     4981 gcgtttttct tgctaaaatc acaagtattg atgagcacga tgcaagaaag atcggaagaa
     5041 ggtttgggtt tgaggctcag tggaaggtga gtagaagttg ataatttgaa agtggagtag
     5101 tgtctatggg gtttttgcct taaatgacag aatacattcc caatatacca aacataactg
     5161 tttcctacta gtcggccgta cgggcccttt cgtctcgcgc gtttcggtga tgacggtgaa
     5221 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg
     5281 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac
     5341 tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac
     5401 agatgcgtaa ggagaaaata ccgcatcagg cggccttaag ggcctcgtga tacgcctatt
     5461 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
     5521 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     5581 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     5641 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     5701 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     5761 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     5821 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     5881 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     5941 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     6001 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     6061 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     6121 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     6181 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     6241 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     6301 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     6361 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     6421 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     6481 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     6541 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     6601 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     6661 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     6721 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     6781 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     6841 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     6901 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     6961 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     7021 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     7081 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     7141 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     7201 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     7261 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     7321 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     7381 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
     7441 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
     7501 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
     7561 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
     7621 cggataacaa tt
//
