LOCUS       sequence-376081-       15017 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression vector for an inducible 3xFLAG-CSE1L.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15017)
  AUTHORS   Wang L, Paudel BB, McKnight RA, Janes KA
  TITLE     Nucleocytoplasmic transport of active HER2 causes fractional escape 
            from the DCIS-like state.
  JOURNAL   Nat Commun. 2023 Apr 13;14(1):2110. doi: 10.1038/s41467-023-37914-x.
  PUBMED    37055441
REFERENCE   2  (bases 1 to 15017)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-04-13"; volume: "14(1):2110. doi"; pages: " 
            10.1038/s41467-023-37914-x"
COMMENT     Sequence Label: pSLIK 3xFLAG-CSE1L neo
FEATURES             Location/Qualifiers
     source          1..15017
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2614..2638
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        2670..2984
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    2678..2696
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2714..2732
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2749..2767
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2785..2803
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2821..2839
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2856..2874
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2892..2910
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2957..2981
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2994..3059
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    complement(6011..6035)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        6085..7296
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     7133..7152
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             join(7355..7972,7973..7978,7979..8014,8015..8017,8018..8053,
                     8054..8056,8057..8092,8093..8101)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    8154..8727
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(8321..8338)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     8548..8567
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             8743..9537
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             8743..9537
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8797..8816)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9407..9426
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    9705..10293
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9758..9778)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10176..10187)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(10296..10312)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10297..10313)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             10822..11002
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(11028..11045)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    11034..11258
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      11304..11732
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11391..11410)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11601..11622
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(11741..11761)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        11746..12075
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      11926..12061
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     11988..12007
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        12123..12170
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             12189..12563
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    12693..12814
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12730..12749)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12784..12803
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(12863..12879)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12863..12879)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12876..12898)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12887..12903
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12911..12917,12918..12935,12936..12941))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12956..12977
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(13094..13111)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(13265..13853)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13345..13364)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(14024..14815,14816..14884))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14647..14666
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(14885..14989)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaattc tagacagtta tcaacaagtt tgtacaaaaa agcaggcttt
     2641 aaaggaacca attcagtcga ctggatcccg agtttactcc ctatcagtga tagagaacgt
     2701 atgtcgagtt tactccctat cagtgataga gaacgatgtc gagtttactc cctatcagtg
     2761 atagagaacg tatgtcgagt ttactcccta tcagtgatag agaacgtatg tcgagtttac
     2821 tccctatcag tgatagagaa cgtatgtcga gtttatccct atcagtgata gagaacgtat
     2881 gtcgagttta ctccctatca gtgatagaga acgtatgtcg aggtaggcgt gtacggtggg
     2941 aggcctatat aagcagagct cgtttagtga accgtcagat cgccactaga atggactaca
     3001 aagaccatga cggtgattat aaagatcatg acatcgatta caaggatgac gatgacaaga
     3061 tggaactcag cgatgcaaat ctgcaaacac taacagaata tttaaagaaa acacttgatc
     3121 ctgatcctgc catccgacgt ccagctgaga aatttcttga atctgttgaa ggaaatcaga
     3181 attatccact gttgcttttg acattactgg agaagtccca ggataatgtt atcaaagtat
     3241 gtgcttcagt aacattcaaa aactatatta aaaggaactg gagaattgtt gaagatgaac
     3301 caaacaaaat ttgtgaagcc gatcgagtgg ccattaaagc caacatagtg cacttgatgc
     3361 ttagcagccc agagcaaatt cagaagcagt taagtgatgc aattagcatt attggcagag
     3421 aagattttcc acagaaatgg cctgacttgc tgacagaaat ggtgaatcgc tttcagagtg
     3481 gagatttcca tgttattaat ggagtcctcc gtacagcaca ttcattattt aaaagatacc
     3541 gtcatgaatt taagtcaaac gagttatgga ctgaaattaa gcttgttctg gatgcctttg
     3601 ctttgccttt gactaatctt tttaaggcca ctattgaact ctgcagtacc catgcaaatg
     3661 atgcctctgc cctgaggatt ctgttttctt ccctgatcct gatctcaaaa ttgttctata
     3721 gtttaaactt tcaggatctc cctgaatttt ttgaagataa tatggaaact tggatgaata
     3781 attttcatac tctcttaaca ttggataata agcttttaca aactgatgat gaagaggaag
     3841 ccggcttatt ggagctctta aaatcccaga tttgtgataa tgccgcactc tatgcacaaa
     3901 agtacgatga agaattccag cgatacctgc ctcgttttgt tacagccatc tggaatttac
     3961 tagttacaac gggtcaagag gttaaatatg atttgttggt aagtaatgca attcaatttc
     4021 tggcttcagt ttgtgagaga cctcattata agaatctatt tgaggaccag aacacgctga
     4081 caagtatctg tgaaaaggtt attgtgccta acatggaatt tagagctgct gatgaagaag
     4141 catttgaaga taattctgag gagtacataa ggagagattt ggaaggatct gatattgata
     4201 ctagacgcag ggctgcttgt gatctggtac gaggattatg caagtttttt gagggacctg
     4261 tgacaggaat cttctctggt tatgttaatt ccatgctgca ggaatacgca aaaaatccat
     4321 ctgtcaactg gaaacacaaa gatgcagcca tctacctagt gacatctttg gcatcaaaag
     4381 cccaaacaca gaagcatgga attacacaag caaatgaact tgtaaaccta actgagttct
     4441 ttgtgaatca catcctccct gatttaaaat cagctaatgt gaatgaattt cctgtcctta
     4501 aagctgacgg tatcaaatat attatgattt ttagaaatca agtgccaaaa gaacatcttt
     4561 tagtctcgat tcctctcttg attaatcatc ttcaagctga aagtattgtt gttcatactt
     4621 acgcagctca tgctcttgaa cggctcttta ctatgcgagg gcctaacaat gccactctct
     4681 ttacagctgc agaaatcgca ccgtttgttg agattctgct aacaaacctt ttcaaagctc
     4741 tcacacttcc tggctcttca gaaaatgaat atattatgaa agctatcatg agaagttttt
     4801 ctctcctaca agaagccata atcccctaca tccctactct catcactcag cttacacaga
     4861 agctattagc tgttagtaag aacccaagca aacctcactt taatcactac atgtttgaag
     4921 caatatgttt atccataaga ataacttgca aagctaaccc tgctgctgtt gtaaattttg
     4981 aggaggcttt gtttttggtg tttactgaaa tcttacaaaa tgatgtgcaa gaatttattc
     5041 catacgtctt tcaagtgatg tctttgcttc tggaaacaca caaaaatgac atcccgtctt
     5101 cctatatggc cttatttcct catctccttc agccagtgct ttgggaaaga acaggaaata
     5161 ttcctgctct agtgaggctt cttcaagcat tcttagaacg cggttcaaac acaatagcaa
     5221 gtgctgcagc tgacaaaatt cctgggttac taggtgtctt tcagaagctg attgcatcca
     5281 aagcaaatga ccaccaaggt ttttatcttc taaacagtat aatagagcac atgcctcctg
     5341 aatcagttga ccaatatagg aaacaaatct tcattctgct attccagaga cttcagaatt
     5401 ccaaaacaac caagtttatc aagagttttt tagtctttat taatttgtat tgcataaaat
     5461 atggggcact agcactacaa gaaatatttg atggtataca accaaaaatg tttggaatgg
     5521 ttttggaaaa aattattatt cctgaaattc agaaggtatc tggaaatgta gagaaaaaga
     5581 tctgtgcggt tggcataacc aaattactaa cagaatgtcc cccaatgatg gacactgagt
     5641 ataccaaact gtggactcca ttattacagt ctttgattgg tctttttgag ttacccgaag
     5701 atgataccat tcctgatgag gaacatttta ttgacataga agatacacca ggatatcaga
     5761 ctgccttctc acagttggca tttgctggga aaaaagagca tgatcctgta ggtcaaatgg
     5821 tgaataaccc caaaattcac ctggcacagt cacttcacaa gttgtctacc gcctgtccag
     5881 gaagggttcc atcaatggtg agcaccagcc tgaatgcaga agcgctccag tatctccaag
     5941 ggtaccttca ggcagccagt gtgacactgc tttaacaatt ggaagactaa tgcgttttcg
     6001 agatatctag acccagcttt cttgtacaaa gtggttgata acatctcgag ccataaagat
     6061 ggttaattaa cccacccaag atctggcctc cgcgccgggt tttggcgcct cccgcgggcg
     6121 cccccctcct cacggcgagc gctgccacgt cagacgaagg gcgcagcgag cgtcctgatc
     6181 cttccgcccg gacgctcagg acagcggccc gctgctcata agactcggcc ttagaacccc
     6241 agtatcagca gaaggacatt ttaggacggg acttgggtga ctctagggca ctggttttct
     6301 ttccagagag cggaacaggc gaggaaaagt agtcccttct cggcgattct gcggagggat
     6361 ctccgtgggg cggtgaacgc cgatgattat ataaggacgc gccgggtgtg gcacagctag
     6421 ttccgtcgca gccgggattt gggtcgcggt tcttgtttgt ggatcgctgt gatcgtcact
     6481 tggtgagtag cgggctgctg ggctggccgg ggctttcgtg gccgccgggc cgctcggtgg
     6541 gacggaagcg tgtggagaga ccgccaaggg ctgtagtctg ggtccgcgag caaggttgcc
     6601 ctgaactggg ggttgggggg agcgcagcaa aatggcggct gttcccgagt cttgaatgga
     6661 agacgcttgt gaggcgggct gtgaggtcgt tgaaacaagg tggggggcat ggtgggcggc
     6721 aagaacccaa ggtcttgagg ccttcgctaa tgcgggaaag ctcttattcg ggtgagatgg
     6781 gctggggcac catctgggga ccctgacgtg aagtttgtca ctgactggag aactcggttt
     6841 gtcgtctgtt gcgggggcgg cagttatggc ggtgccgttg ggcagtgcac ccgtaccttt
     6901 gggagcgcgc gccctcgtcg tgtcgtgacg tcacccgttc tgttggctta taatgcaggg
     6961 tggggccacc tgccggtagg tgtgcggtag gcttttctcc gtcgcaggac gcagggttcg
     7021 ggcctagggt aggctctcct gaatcgacag gcgccggacc tctggtgagg ggagggataa
     7081 gtgaggcgtc agtttctttg gtcggtttta tgtacctatc ttcttaagta gctgaagctc
     7141 cggttttgaa ctatgcgctc ggggttggcg agtgtgtttt gtgaagtttt ttaggcacct
     7201 tttgaaatgt aatcatttgg gtcaatatgt aattttcagt gttagactag taaattgtcc
     7261 gctaaattct ggccgttttt ggcttttttg ttagacgaag cttggtaccg agctcggatc
     7321 tccaccccgt accggtcctg cagtcgaatt caccatgtct agactggaca agagcaaagt
     7381 cataaacgga gctctggaat tactcaatgg tgtcggtatc gaaggcctga cgacaaggaa
     7441 actcgctcaa aagctgggag ttgagcagcc taccctgtac tggcacgtga agaacaagcg
     7501 ggccctgctc gatgccctgc caatcgagat gctggacagg catcataccc acttctgccc
     7561 cctggaaggc gagtcatggc aagactttct gcggaacaac gccaagtcat accgctgtgc
     7621 tctcctctca catcgcgacg gggctaaagt gcatctcggc acccgcccaa cagagaaaca
     7681 gtacgaaacc ctggaaaatc agctcgcgtt cctgtgtcag caaggcttct ccctggagaa
     7741 cgcactgtac gctctgtccg ccgtgggcca ctttacactg ggctgcgtat tggaggaaca
     7801 ggagcatcaa gtagcaaaag aggaaagaga gacacctacc accgattcta tgcccccact
     7861 tctgagacaa gcaattgagc tgttcgaccg gcagggagcc gaacctgcct tccttttcgg
     7921 cctggaacta atcatatgtg gcctggagaa acagctaaag tgcgaaagcg gcgggccgac
     7981 cgacgccctt gacgattttg acttagacat gctcccagcc gatgcccttg acgactttga
     8041 ccttgatatg ctgcctgctg acgctcttga cgattttgac cttgacatgc tccccgggta
     8101 actaagtaag gatccgcggc cgcactagag gaattccgcc cctctccccc ccccccctct
     8161 ccctcccccc cccctaacgt tactggccga agccgcttgg aataaggccg gtgtgcgttt
     8221 gtctatatgt tattttccac catattgccg tcttttggca atgtgagggc ccggaaacct
     8281 ggccctgtct tcttgacgag cattcctagg ggtctttccc ctctcgccaa aggaatgcaa
     8341 ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag acaaacaacg
     8401 tctgtagcga ccctttgcag gcagcggaac cccccacctg gcgacaggtg cctctgcggc
     8461 caaaagccac gtgtataaga tacacctgca aaggcggcac aaccccagtg ccacgttgtg
     8521 agttggatag ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg
     8581 aaggatgccc agaaggtacc ccattgtatg ggatctgatc tggggcctcg gtgcacatgc
     8641 tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg
     8701 gttttccttt gaaaaacacg atgataagct taccggtcca ccatgattga acaagatgga
     8761 ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa
     8821 cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt
     8881 ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaagacga ggcagcgcgg
     8941 ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa
     9001 gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac
     9061 cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt
     9121 gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact
     9181 cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg
     9241 ccagccgaac tgttcgccag gctcaaggcg agcatgcccg acggcgagga tctcgtcgtg
     9301 acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc
     9361 atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt
     9421 gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc
     9481 gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgatgt
     9541 acaagtaaag cggccgcgac tctagatcat aatcagccat accacatttg tagaggtttt
     9601 acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat
     9661 tgttgttgtt tagtccctcc caattcgata tcaagcttat cgataatcaa cctctggatt
     9721 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
     9781 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
     9841 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
     9901 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
     9961 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
    10021 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
    10081 ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct
    10141 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
    10201 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga
    10261 cgagtcggat ctccctttgg gccgcctccc cgcatcgata ccgtcgacct cgatcgagac
    10321 ctagaaaaac atggagcaat cacaagtagc aatacagcag ctaccaatgc tgattgtgcc
    10381 tggctagaag cacaagagga ggaggaggtg ggttttccag tcacacctca ggtaccttta
    10441 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
    10501 ctggaagggc taattcactc ccaacgaaga caagatatcc ttgatctgtg gatctaccac
    10561 acacaaggct acttccctga ttggcagaac tacacaccag ggccagggat cagatatcca
    10621 ctgacctttg gatggtgcta caagctagta ccagttgagc aagagaaggt agaagaagcc
    10681 aatgaaggag agaacacccg cttgttacac cctgtgagcc tgcatgggat ggatgacccg
    10741 gagagagaag tattagagtg gaggtttgac agccgcctag catttcatca catggcccga
    10801 gagctgcatc cggactgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
    10861 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
    10921 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
    10981 tcagtgtgga aaatctctag cagggcccgt ttaaacccgc tgatcagcct cgactgtgcc
    11041 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
    11101 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
    11161 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga
    11221 caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag
    11281 ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
    11341 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
    11401 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
    11461 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
    11521 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
    11581 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
    11641 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
    11701 tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg
    11761 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
    11821 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
    11881 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
    11941 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
    12001 gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
    12061 ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcagcac
    12121 gtgttgacaa ttaatcatcg gcatagtata tcggcatagt ataatacgac aaggtgagga
    12181 actaaaccat ggccaagttg accagtgccg ttccggtgct caccgcgcgc gacgtcgccg
    12241 gagcggtcga gttctggacc gaccggctcg ggttctcccg ggacttcgtg gaggacgact
    12301 tcgccggtgt ggtccgggac gacgtgaccc tgttcatcag cgcggtccag gaccaggtgg
    12361 tgccggacaa caccctggcc tgggtgtggg tgcgcggcct ggacgagctg tacgccgagt
    12421 ggtcggaggt cgtgtccacg aacttccggg acgcctccgg gccggccatg accgagatcg
    12481 gcgagcagcc gtgggggcgg gagttcgccc tgcgcgaccc ggccggcaac tgcgtgcact
    12541 tcgtggccga ggagcaggac tgacacgtgc tacgagattt cgattccacc gccgccttct
    12601 atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg
    12661 gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt
    12721 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
    12781 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta
    12841 gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
    12901 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
    12961 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
    13021 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
    13081 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
    13141 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
    13201 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
    13261 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
    13321 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
    13381 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
    13441 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
    13501 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
    13561 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
    13621 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
    13681 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
    13741 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
    13801 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
    13861 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
    13921 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
    13981 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
    14041 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
    14101 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
    14161 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
    14221 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
    14281 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
    14341 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
    14401 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
    14461 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
    14521 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
    14581 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
    14641 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
    14701 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
    14761 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
    14821 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
    14881 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
    14941 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
    15001 gaaaagtgcc acctgac
//
