LOCUS       sequence-376083-       11648 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Reporter circuit for hijacking mRNA of zraP as CRISPR RNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11648)
  AUTHORS   Liu Y, Pinto F, Wan X, Yang Z, Peng S, Li M, Cooper JM, Xie Z, 
            French CE, Wang B
  TITLE     Reprogrammed tracrRNAs enable repurposing of RNAs as crRNAs and 
            sequence-specific RNA biosensors.
  JOURNAL   Nat Commun. 2022 Apr 11;13(1):1937. doi: 10.1038/s41467-022-29604-x.
  PUBMED    35410423
REFERENCE   2  (bases 1 to 11648)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-04-11"; volume: "13(1):1937. doi"; pages: " 
            10.1038/s41467-022-29604-x"
COMMENT     Sequence Label: pLY305
FEATURES             Location/Qualifiers
     source          1..11648
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(45..56)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             complement(71..697)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MMSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGT
                     RPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPT
                     TDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     602..621
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     promoter        713..768
                     /gene="tetR"
                     /label=tetR/tetA promoters
                     /note="overlapping promoters for bacterial tetR and tetA"
     protein_bind    749..767
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             786..797
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             join(804..830,831..833,834..3320,3321..3323,3324..4910)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     misc_feature    4974..4995
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      4998..5041
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(5045..5063)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5128..5232
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5233..5301,5302..6093)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5451..5470)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     terminator      6123..6217
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      6709..6931
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             6979..7929
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     primer_bind     8191..8208
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(8232..8289)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     misc_feature    8290..8310
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     CDS             complement(8417..9130)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHNLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     RBS             9137..9148
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     misc_RNA        complement(9181..9230)
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     CDS             complement(9433..9444)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        complement(10442..10560)
                     /label=rhaB promoter
                     /note="promoter of the E. coli rhaBAD operon, conferring 
                     tight induction with L-rhamnose and repression with 
                     D-glucose in the presence of RhaR and RhaS (Giacalone et 
                     al., 2006)"
     terminator      complement(10618..10645)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     promoter        10661..10695
                     /label=BBa_J23101
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.7"
     CDS             10713..11549
                     /codon_start=1
                     /gene="E. coli rhaS"
                     /product="positive regulator of the rhaB promoter
                     "
                     /label=rhaS
                     /note="activated by L-rhamnose"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHGT
                     GIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLLPQ
                     ELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKSSLQ
                     ENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQRYLNR
                     LRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGFLQ"
ORIGIN
        1 ctaggctcta gtagggagac cagaaacaaa aaaaggccgc gttagcggcc ttcaataatt
       61 ggctctagta ttaagaccca ctttcacatt taagttgttt ttctaatccg catatgatca
      121 attcaaggcc gaataagaag gctggctctg caccttggtg atcaaataat tcgatagctt
      181 gtcgtaataa tggcggcata ctatcagtag taggtgtttc cctttcttct ttagcgactt
      241 gatgctcttg atcttccaat acgcaaccta aagtaaaatg ccccacagcg ctgagtgcat
      301 ataatgcatt ctctagtgaa aaaccttgtt ggcataaaaa ggctaattga ttttcgagag
      361 tttcatactg tttttctgta ggccgtgtac ctaaatgtac ttttgctcca tcgcgatgac
      421 ttagtaaagc acatctaaaa cttttagcgt tattacgtaa aaaatcttgc cagctttccc
      481 cttctaaagg gcaaaagtga gtatggtgcc tatctaacat ctcaatggct aaggcgtcga
      541 gcaaagcccg cttatttttt acatgccaat acaatgtagg ctgctctaca cctagcttct
      601 gggcgagttt acgggttgtt aaaccttcga ttccgacctc attaagcagc tctaatgcgc
      661 tgttaatcac tttactttta tctaatcttg acatcattaa ttcctaattt ttgttgacac
      721 tctatcgttg atagagttat tttaccactc cctatcagtg atagagaaaa gaattgaaaa
      781 gatctaaaga ggagaaagga tctatggata agaaatactc aataggctta gctatcggca
      841 caaatagcgt cggatgggcg gtgatcactg atgaatataa ggttccgtct aaaaagttca
      901 aggttctggg aaatacagac cgccacagta tcaaaaaaaa tcttataggg gctcttttat
      961 ttgacagtgg agagacagcg gaagcgactc gcctcaaacg gacagctcgt agaaggtata
     1021 cacgtcggaa gaatcgtatt tgttatctac aggagatttt ttcaaatgag atggcgaaag
     1081 tagatgatag tttctttcat cgacttgaag agtctttttt ggtggaagaa gacaagaagc
     1141 atgaacgtca tcctattttt ggaaatatag tagatgaagt tgcttatcat gagaaatatc
     1201 caactatcta tcatctgcga aaaaaattgg tagattctac tgataaagcg gatttgcgct
     1261 taatctattt ggccttagcg catatgatta agtttcgtgg tcattttttg attgagggag
     1321 atttaaatcc tgataatagt gatgtggaca aactatttat ccagttggta caaacctaca
     1381 atcaattatt tgaagaaaac cctattaacg caagtggagt agatgctaaa gcgattcttt
     1441 ctgcacgatt gagtaaatca agacgattag aaaatctcat tgctcagctc cccggtgaga
     1501 agaaaaatgg cttatttggg aatctcattg ctttgtcatt gggtttgacc cctaatttta
     1561 aatcaaattt tgatttggca gaagatgcta aattacagct ttcaaaagat acttacgatg
     1621 atgatttaga taatttattg gcgcaaattg gagatcaata tgctgatttg tttttggcag
     1681 ctaagaattt atcagatgct attttacttt cagatatcct aagagtaaat actgaaataa
     1741 ctaaggctcc cctatcagct tcaatgatta aacgctacga tgaacatcat caagacttga
     1801 ctcttttaaa agctttagtt cgacaacaac ttccagaaaa gtataaagaa atcttttttg
     1861 atcaatcaaa aaacggatat gcaggttata ttgatggggg agctagccaa gaagaatttt
     1921 ataaatttat caaaccaatt ttagaaaaaa tggatggtac tgaggaatta ttggtgaaac
     1981 taaatcgtga agatttgctg cgcaagcaac ggacctttga caacggctct attccccatc
     2041 aaattcactt gggtgagctg catgctattt tgagaagaca agaagacttt tatccatttt
     2101 taaaagacaa tcgtgagaag attgaaaaaa tcttgacttt tcgtattcct tattatgttg
     2161 gtccattggc gcgtggcaat agtcgttttg catggatgac tcggaagtct gaagaaacaa
     2221 ttaccccatg gaattttgaa gaagttgtcg ataaaggtgc ttcagctcaa tcatttattg
     2281 aacgcatgac aaactttgat aaaaatcttc caaatgaaaa agtactacca aaacatagtt
     2341 tgctttatga gtattttacg gtttataacg aattgacaaa ggtcaaatat gttactgaag
     2401 gaatgcgaaa accagcattt ctttcaggtg aacagaagaa agccattgtt gatttactct
     2461 tcaaaacaaa tcgaaaagta accgttaagc aattaaaaga agattatttc aaaaaaatag
     2521 aatgttttga tagtgttgaa atttcaggag ttgaagatag atttaatgct tcattaggta
     2581 cctaccatga tttgctaaaa attattaaag ataaagattt tttggataat gaagaaaatg
     2641 aagatatctt agaggatatt gttttaacat tgaccttatt tgaagatagg gagatgattg
     2701 aggaaagact taaaacatat gctcacctct ttgatgataa ggtgatgaaa cagcttaaac
     2761 gtcgccgtta tactggttgg ggacgtttgt ctcgaaaatt gattaatggt attagggata
     2821 agcaatctgg caaaacaata ttagattttt tgaaatcaga tggttttgcc aatcgcaatt
     2881 ttatgcagct gatccatgat gatagtttga catttaaaga agacattcaa aaagcacaag
     2941 tgtctggaca aggcgatagt ttacatgaac atattgcaaa tttagctggt agccctgcta
     3001 ttaaaaaagg tattttacag actgtaaaag ttgttgatga attggtcaaa gtaatggggc
     3061 ggcataagcc agaaaatatc gttattgaaa tggcacgtga aaatcagaca actcaaaagg
     3121 gccagaaaaa ttcgcgagag cgtatgaaac gaatcgaaga aggtatcaaa gaattaggaa
     3181 gtcagattct taaagagcat cctgttgaaa atactcaatt gcaaaatgaa aagctctatc
     3241 tctattatct ccaaaatgga agagacatgt atgtggacca agaattagat attaatcgtt
     3301 taagtgatta tgatgtcgat gccattgttc cacaaagttt ccttaaagac gattcaatag
     3361 acaataaggt cttaacgcgt tctgataaaa atcgtggtaa atcggataac gttccaagtg
     3421 aagaagtagt caaaaagatg aaaaactatt ggagacaact tctaaacgcc aagttaatca
     3481 ctcaacgtaa gtttgataat ttaacgaaag ctgaacgtgg aggtttgagt gaacttgata
     3541 aagctggttt tatcaaacgc caattggttg aaactcgcca aatcactaag catgtggcac
     3601 aaattttgga tagtcgcatg aatactaaat acgatgaaaa tgataaactt attcgagagg
     3661 ttaaagtgat taccttaaaa tctaaattag tttctgactt ccgaaaagat ttccaattct
     3721 ataaagtacg tgagattaac aattaccatc atgcccatga tgcgtatcta aatgccgtcg
     3781 ttggaactgc tttgattaag aaatatccaa aacttgaatc ggagtttgtc tatggtgatt
     3841 ataaagttta tgatgttcgt aaaatgattg ctaagtctga gcaagaaata ggcaaagcaa
     3901 ccgcaaaata tttcttttac tctaatatca tgaacttctt caaaacagaa attacacttg
     3961 caaatggaga gattcgcaaa cgccctctaa tcgaaactaa tggggaaact ggagaaattg
     4021 tctgggataa agggcgagat tttgccacag tgcgcaaagt attgtccatg ccccaagtca
     4081 atattgtcaa gaaaacagaa gtacagacag gcggattctc caaggagtca attttaccaa
     4141 aaagaaattc ggacaagctt attgctcgta aaaaagactg ggatccaaaa aaatatggtg
     4201 gttttgatag tccaacggta gcttattcag tcctagtggt tgctaaggtg gaaaaaggga
     4261 aatcgaagaa gttaaaatcc gttaaagagt tactagggat cacaattatg gaaagaagtt
     4321 cctttgaaaa aaatccgatt gactttttag aagctaaagg atataaggaa gttaaaaaag
     4381 acttaatcat taaactacct aaatatagtc tttttgagtt agaaaacggt cgtaaacgga
     4441 tgctggctag tgccggagaa ttacaaaaag gaaatgagct ggctctgcca agcaaatatg
     4501 tgaatttttt atatttagct agtcattatg aaaagttgaa gggtagtcca gaagataacg
     4561 aacaaaaaca attgtttgtg gagcagcata agcattattt agatgagatt attgagcaaa
     4621 tcagtgaatt ttctaagcgt gttattttag cagatgccaa tttagataaa gttcttagtg
     4681 catataacaa acatagagac aaaccaatac gtgaacaagc agaaaatatt attcatttat
     4741 ttacgttgac gaatcttgga gctcccgctg cttttaaata ttttgataca acaattgatc
     4801 gtaaacgata tacgtctaca aaagaagttt tagatgccac tcttatccat caatccatca
     4861 ctggtcttta tgaaacacgc attgatttga gtcagctagg aggtgactaa tactagagcc
     4921 aattattgaa ggggagcggg aaaccgctcc cctttttttg tttctggtct cccctctaga
     4981 agcggccgcg aattccagaa atcatcctta gcgaaagcta aggatttttt ttatctgaaa
     5041 ttctgcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
     5101 acgtcaggtg gcaccatggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     5161 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     5221 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     5281 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     5341 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     5401 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     5461 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     5521 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     5581 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     5641 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     5701 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     5761 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     5821 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     5881 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     5941 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     6001 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     6061 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta cgagctcgct
     6121 tggactcctg ttgatagatc cagtaatgac ctcagaactc catctggatt tgttcagaac
     6181 gctcggttgc cgccgggcgt tttttattgg tgagaatcca agcactaggg acagtaagac
     6241 gggtaagcct gttgatgata ccgctgcctt actgggtgca ttagccagtc tgaatgacct
     6301 gtcacgggat aatccgaagt ggtcagactg gaaaatcaga gggcaggaac tgctgaacag
     6361 caaaaagtca gatagcacca catagcagac ccgccataaa acgccctgag aagcccgtga
     6421 cgggcttttc ttgtattatg ggtagtttcc ttgcatgaat ccataaaagg cgcctgtagt
     6481 gccatttacc cccattcact gccagagccg tgagcgcagc gaactgaatg tcacgaaaaa
     6541 gacagcgact caggtgcctg atggtcggag acaaaaggaa tattcagcga tttgcccgag
     6601 cttgcgaggg tgctacttaa gcctttaggg ttttaaggtc tgttttgtag aggagcaaac
     6661 agcgtttgcg acatcctttt gtaatactgc ggaactgact aaagtagtga gttatacaca
     6721 gggctgggat ctattctttt tatctttttt tattctttct ttattctata aattataacc
     6781 acttgaatat aaacaaaaaa aacacacaaa ggtctagcgg aatttacaga gggtctagca
     6841 gaatttacaa gttttccagc aaaggtctag cagaatttac agatacccac aactcaaagg
     6901 aaaaggacat gtaattatca ttgactagcc catctcaatt ggtatagtga ttaaaatcac
     6961 ctagaccaat tgagatgtat gtctgaatta gttgttttca aagcaaatga actagcgatt
     7021 agtcgctatg acttaacgga gcatgaaacc aagctaattt tatgctgtgt ggcactactc
     7081 aaccccacga ttgaaaaccc tacaaggaaa gaacggacgg tatcgttcac ttataaccaa
     7141 tacgctcaga tgatgaacat cagtagggaa aatgcttatg gtgtattagc taaagcaacc
     7201 agagagctga tgacgagaac tgtggaaatc aggaatcctt tggttaaagg ctttgagatt
     7261 ttccagtgga caaactatgc caagttctca agcgaaaaat tagaattagt ttttagtgaa
     7321 gagatattgc cttatctttt ccagttaaaa aaattcataa aatataatct ggaacatgtt
     7381 aagtcttttg aaaacaaata ctctatgagg atttatgagt ggttattaaa agaactaaca
     7441 caaaagaaaa ctcacaaggc aaatatagag attagccttg atgaatttaa gttcatgtta
     7501 atgcttgaaa ataactacca tgagtttaaa aggcttaacc aatgggtttt gaaaccaata
     7561 agtaaagatt taaacactta cagcaatatg aaattggtgg ttgataagcg aggccgcccg
     7621 actgatacgt tgattttcca agttgaacta gatagacaaa tggatctcgt aaccgaactt
     7681 gagaacaacc agataaaaat gaatggtgac aaaataccaa caaccattac atcagattcc
     7741 tacctacata acggactaag aaaaacacta cacgatgctt taactgcaaa aattcagctc
     7801 accagttttg aggcaaaatt tttgagtgac atgcaaagta agtatgatct caatggttcg
     7861 ttctcatggc tcacgcaaaa acaacgaacc acactagaga acatactggc taaatacgga
     7921 aggatctgag gttcttatgg ctcttgtatc tatcagtgaa gcatcaagac taacaaacaa
     7981 aagtagaaca actgttcacc gttacatatc aaagggaaaa ctgtccatat gcacagatga
     8041 aaacggtgta aaaaagatag atacatcaga gcttttacga gtttttggtg cattcaaagc
     8101 tgttcaccat gaacagatcg acaatgtaac ctcgagatta ccgcctttga gtgagctgat
     8161 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcctgcata
     8221 acgcgaagta atcttttcgg ttttaaagaa aaagggcagg gtggtgacac cttgcccttt
     8281 tttgccggac tgcagcggcc gctactagta gggagaccag aaacaaaaaa aggccccccg
     8341 ttagggaggc cttcaataat tggctctagt attattaaac tgatgcagcg tagttttcgt
     8401 cgtttgcagc aggccttttg tacagttcat ccataccatg cgtgatgccc gctgcggtta
     8461 cgaactccag cagaaccata tgatcgcgtt tctcgttcgg atctttagac agaacgcttt
     8521 gcgtgctcag atagtgattg tctggcagca gaacaggacc atcaccgatt ggagtgtttt
     8581 gctggtagtg atcagccagc tgcacgctgc catcctccac gttgtggcga attttaaaat
     8641 tcgctttaat gccatttttt tgtttatcgg cggtgatgta aacattgtgg ctgttaaaat
     8701 tgtattccag gttatggccc aggatattgc cgtcttcttt aaagtcaatg cctttcagct
     8761 caatgcggtt taccagggta tcgccttcaa atttcacttc cgcacgcgtt ttgtacgtgc
     8821 cgtcatcctt aaaggaaatc gtgcgttcct gcacatagcc ttccggcatg gcggacttga
     8881 agaagtcatg ctgcttcata tggtccggat aacgagcaaa gcactgaaca ccataagtca
     8941 gcgtcgttac cagagtcggc caaggtaccg gcagtttacc agtagtacag atgaacttca
     9001 gcgtcagttt accattagtt gcgtcacctt caccctcgcc acgcacggaa aacttatgac
     9061 cgttgacatc accatccagt tccaccagaa tagggacgac accagtgaac agctcttcgc
     9121 ctttacgcat atggtatttc tcctctttaa tctctagtta aacaaaatta tttgtagagg
     9181 ctgtttcgtc ctcacggact catcagaccg gaaagcacat ccggtgacag ctctctagta
     9241 tttgcgttca ggattgtcct gctgaactgt taatacaatt tgcgtgccaa ttttttatct
     9301 ttttgattta taaagatctg attgaagaat caacagcaac atgccaggat gagttagcga
     9361 attacactaa ccgaatcctc cagtggttgt cttctctagt agggagacca gaaacaaaaa
     9421 aaggccgcgt tagcggcctt caataattgg ctctagtatt aatttgctgc tttccattga
     9481 gcctgtttct ctgcgcgacg ttcgcggcgg cgtgtgcgtg cattcatttg caaactgagt
     9541 tgcagcaact ctttttcctg ctgcatctga aactcacgta aatccagcgg cagtgttgga
     9601 agcgaggtgg tttctgaaac ggcgatagcg tcttcaggcg gacgccgttt aaagggatca
     9661 ataatgatgt catcaagcgg ataatcgctg gtgccgtggc gatacactga acgttccacc
     9721 acgtttttca attcacgaat atttcccggc caacgataat tcagcaatgt ttctctggcg
     9781 cgctccgtaa accccgggaa cagaggcagc ttgatttccc gacacatctg gatggcaaag
     9841 tattctgcca tcaacattat gtcgctttcg cgctcgcgca gtggtggcag ttgtacaaca
     9901 tcaaaagcca gtcggtcgag caggtcagcg cgaaaagtgc cttcattgac catcgccggg
     9961 agatcggcat tcgtcgcgca taccaaccgc acattcacct gcaatggttg gctgccgcca
    10021 acgcgctcca gttcaccgta ctcaatcacg cgcaataatt tctcctgcac catcatcggt
    10081 gccgtagcga gttcatcaag aaatagcgta ccgccgtcgg cacgttcaaa tctccctgga
    10141 tgacgttttt gcgcaccggt aaacgccccc gcttcgtgac caaacagttc ggaatccagc
    10201 agattttcat ttaacgccgc gcagttaagg gaaataaacg gcccttgcca acgggaggag
    10261 agataatgca ggcggctggc aatcagctct ttaccggtgc cgcgttcgcc gatgatgagc
    10321 accggtttgt ccagcggtgc gagatgcgaa acctgttcca gcacttcgag aaagctgttc
    10381 gcctcaccaa gtaaattatc tttgtattct gccatctagt actttcctgt gtgactctag
    10441 ttacgaccag tctaaaaagc gcctcaattc gcgaccttct cgttactgac aggaaaatgg
    10501 gccattggca accagggaaa gatgaacgtg atgatgttca caatttgctg aattgtggtg
    10561 atgtgatgct caccgcattc ctgctccagc ggcgtaaagg tctctctagt atataaacgc
    10621 agaaaggccc acccgaaggt gagccagtgt gatactagag tttacagcta gctcagtcct
    10681 aggtattatg ctagctacta gagggagccc aaatgaccgt attacatagt gtggattttt
    10741 ttccgtccgg taacgcgtcc gtggcgatag aaccccggct cccgcaggcg gattttcctg
    10801 aacatcatca tgattttcat gaaattgtga ttgtcgaaca tggcacgggt attcatgtgt
    10861 ttaatgggca gccctatacc atcaccggtg gcacggtctg tttcgtacgc gatcatgatc
    10921 ggcatctgta tgaacatacc gataatctgt gtctgaccaa tgtgctgtat cgctcgccgg
    10981 atcgatttca gtttctcgcc gggctgaatc agttgctgcc acaagagctg gatgggcagt
    11041 atccgtctca ctggcgcgtt aaccacagcg tattgcagca ggtgcgacag ctggttgcac
    11101 agatggaaca gcaggaaggg gaaaatgatt taccctcgac cgccagtcgc gagatcttgt
    11161 ttatgcaatt actgctcttg ctgcgtaaaa gcagtttgca ggagaacctg gaaaacagcg
    11221 catcacgtct caacttgctt ctggcctggc tggaggacca ttttgccgat gaggtgaatt
    11281 gggatgccgt ggcggatcaa ttttctcttt cactgcgtac gctacatcgg cagcttaagc
    11341 agcaaacggg actgacgcct cagcgatacc tgaaccgcct gcgactgatg aaagcccgac
    11401 atctgctacg ccacagcgag gccagcgtta ctgacatcgc ctatcgctgt ggattcagcg
    11461 acagtaacca cttttcgacg ctttttcgcc gagagtttaa ctggtcaccg cgtgatattc
    11521 gccagggacg ggatggcttt ctgcaataat actagctcgg taccaaagac gaacaataag
    11581 acgctgaaaa gcgtcttttt tcgttttggt ccctctagta ttaattttgt cttcatgcat
    11641 gaagacaa
//
