LOCUS       sequence-376084-       11648 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Reporter circuit for hijacking small RNA oxyS as CRISPR RNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11648)
  AUTHORS   Liu Y, Pinto F, Wan X, Yang Z, Peng S, Li M, Cooper JM, Xie Z, 
            French CE, Wang B
  TITLE     Reprogrammed tracrRNAs enable repurposing of RNAs as crRNAs and 
            sequence-specific RNA biosensors.
  JOURNAL   Nat Commun. 2022 Apr 11;13(1):1937. doi: 10.1038/s41467-022-29604-x.
  PUBMED    35410423
REFERENCE   2  (bases 1 to 11648)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-04-11"; volume: "13(1):1937. doi"; pages: " 
            10.1038/s41467-022-29604-x"
COMMENT     Sequence Label: pLY300
FEATURES             Location/Qualifiers
     source          1..11648
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(34..45)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             complement(60..686)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MMSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGT
                     RPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPT
                     TDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     591..610
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     promoter        702..757
                     /gene="tetR"
                     /label=tetR/tetA promoters
                     /note="overlapping promoters for bacterial tetR and tetA"
     protein_bind    738..756
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             775..786
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             join(793..819,820..822,823..3309,3310..3312,3313..4899)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     misc_feature    4963..4984
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      4987..5030
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(5034..5052)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5117..5221
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5222..5290,5291..6082)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5440..5459)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     terminator      6112..6206
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      6698..6920
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             6968..7918
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     primer_bind     8180..8197
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(8221..8278)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     misc_feature    8279..8299
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     CDS             complement(8406..9119)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     RBS             9126..9137
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     misc_RNA        complement(9170..9219)
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     CDS             complement(9422..9433)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        complement(10431..10549)
                     /label=rhaB promoter
                     /note="promoter of the E. coli rhaBAD operon, conferring 
                     tight induction with L-rhamnose and repression with 
                     D-glucose in the presence of RhaR and RhaS (Giacalone et 
                     al., 2006)"
     terminator      complement(10607..10634)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     promoter        10650..10684
                     /label=BBa_J23101
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.7"
     CDS             10702..11538
                     /codon_start=1
                     /gene="E. coli rhaS"
                     /product="positive regulator of the rhaB promoter
                     "
                     /label=rhaS
                     /note="activated by L-rhamnose"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHGT
                     GIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLLPQ
                     ELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKSSLQ
                     ENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQRYLNR
                     LRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGFLQ"
ORIGIN
        1 tagggagacc agaaacaaaa aaaggccgcg ttagcggcct tcaataattg gctctagtat
       61 taagacccac tttcacattt aagttgtttt tctaatccgc atatgatcaa ttcaaggccg
      121 aataagaagg ctggctctgc accttggtga tcaaataatt cgatagcttg tcgtaataat
      181 ggcggcatac tatcagtagt aggtgtttcc ctttcttctt tagcgacttg atgctcttga
      241 tcttccaata cgcaacctaa agtaaaatgc cccacagcgc tgagtgcata taatgcattc
      301 tctagtgaaa aaccttgttg gcataaaaag gctaattgat tttcgagagt ttcatactgt
      361 ttttctgtag gccgtgtacc taaatgtact tttgctccat cgcgatgact tagtaaagca
      421 catctaaaac ttttagcgtt attacgtaaa aaatcttgcc agctttcccc ttctaaaggg
      481 caaaagtgag tatggtgcct atctaacatc tcaatggcta aggcgtcgag caaagcccgc
      541 ttatttttta catgccaata caatgtaggc tgctctacac ctagcttctg ggcgagttta
      601 cgggttgtta aaccttcgat tccgacctca ttaagcagct ctaatgcgct gttaatcact
      661 ttacttttat ctaatcttga catcattaat tcctaatttt tgttgacact ctatcgttga
      721 tagagttatt ttaccactcc ctatcagtga tagagaaaag aattgaaaag atctaaagag
      781 gagaaaggat ctatggataa gaaatactca ataggcttag ctatcggcac aaatagcgtc
      841 ggatgggcgg tgatcactga tgaatataag gttccgtcta aaaagttcaa ggttctggga
      901 aatacagacc gccacagtat caaaaaaaat cttatagggg ctcttttatt tgacagtgga
      961 gagacagcgg aagcgactcg cctcaaacgg acagctcgta gaaggtatac acgtcggaag
     1021 aatcgtattt gttatctaca ggagattttt tcaaatgaga tggcgaaagt agatgatagt
     1081 ttctttcatc gacttgaaga gtcttttttg gtggaagaag acaagaagca tgaacgtcat
     1141 cctatttttg gaaatatagt agatgaagtt gcttatcatg agaaatatcc aactatctat
     1201 catctgcgaa aaaaattggt agattctact gataaagcgg atttgcgctt aatctatttg
     1261 gccttagcgc atatgattaa gtttcgtggt cattttttga ttgagggaga tttaaatcct
     1321 gataatagtg atgtggacaa actatttatc cagttggtac aaacctacaa tcaattattt
     1381 gaagaaaacc ctattaacgc aagtggagta gatgctaaag cgattctttc tgcacgattg
     1441 agtaaatcaa gacgattaga aaatctcatt gctcagctcc ccggtgagaa gaaaaatggc
     1501 ttatttggga atctcattgc tttgtcattg ggtttgaccc ctaattttaa atcaaatttt
     1561 gatttggcag aagatgctaa attacagctt tcaaaagata cttacgatga tgatttagat
     1621 aatttattgg cgcaaattgg agatcaatat gctgatttgt ttttggcagc taagaattta
     1681 tcagatgcta ttttactttc agatatccta agagtaaata ctgaaataac taaggctccc
     1741 ctatcagctt caatgattaa acgctacgat gaacatcatc aagacttgac tcttttaaaa
     1801 gctttagttc gacaacaact tccagaaaag tataaagaaa tcttttttga tcaatcaaaa
     1861 aacggatatg caggttatat tgatggggga gctagccaag aagaatttta taaatttatc
     1921 aaaccaattt tagaaaaaat ggatggtact gaggaattat tggtgaaact aaatcgtgaa
     1981 gatttgctgc gcaagcaacg gacctttgac aacggctcta ttccccatca aattcacttg
     2041 ggtgagctgc atgctatttt gagaagacaa gaagactttt atccattttt aaaagacaat
     2101 cgtgagaaga ttgaaaaaat cttgactttt cgtattcctt attatgttgg tccattggcg
     2161 cgtggcaata gtcgttttgc atggatgact cggaagtctg aagaaacaat taccccatgg
     2221 aattttgaag aagttgtcga taaaggtgct tcagctcaat catttattga acgcatgaca
     2281 aactttgata aaaatcttcc aaatgaaaaa gtactaccaa aacatagttt gctttatgag
     2341 tattttacgg tttataacga attgacaaag gtcaaatatg ttactgaagg aatgcgaaaa
     2401 ccagcatttc tttcaggtga acagaagaaa gccattgttg atttactctt caaaacaaat
     2461 cgaaaagtaa ccgttaagca attaaaagaa gattatttca aaaaaataga atgttttgat
     2521 agtgttgaaa tttcaggagt tgaagataga tttaatgctt cattaggtac ctaccatgat
     2581 ttgctaaaaa ttattaaaga taaagatttt ttggataatg aagaaaatga agatatctta
     2641 gaggatattg ttttaacatt gaccttattt gaagataggg agatgattga ggaaagactt
     2701 aaaacatatg ctcacctctt tgatgataag gtgatgaaac agcttaaacg tcgccgttat
     2761 actggttggg gacgtttgtc tcgaaaattg attaatggta ttagggataa gcaatctggc
     2821 aaaacaatat tagatttttt gaaatcagat ggttttgcca atcgcaattt tatgcagctg
     2881 atccatgatg atagtttgac atttaaagaa gacattcaaa aagcacaagt gtctggacaa
     2941 ggcgatagtt tacatgaaca tattgcaaat ttagctggta gccctgctat taaaaaaggt
     3001 attttacaga ctgtaaaagt tgttgatgaa ttggtcaaag taatggggcg gcataagcca
     3061 gaaaatatcg ttattgaaat ggcacgtgaa aatcagacaa ctcaaaaggg ccagaaaaat
     3121 tcgcgagagc gtatgaaacg aatcgaagaa ggtatcaaag aattaggaag tcagattctt
     3181 aaagagcatc ctgttgaaaa tactcaattg caaaatgaaa agctctatct ctattatctc
     3241 caaaatggaa gagacatgta tgtggaccaa gaattagata ttaatcgttt aagtgattat
     3301 gatgtcgatg ccattgttcc acaaagtttc cttaaagacg attcaataga caataaggtc
     3361 ttaacgcgtt ctgataaaaa tcgtggtaaa tcggataacg ttccaagtga agaagtagtc
     3421 aaaaagatga aaaactattg gagacaactt ctaaacgcca agttaatcac tcaacgtaag
     3481 tttgataatt taacgaaagc tgaacgtgga ggtttgagtg aacttgataa agctggtttt
     3541 atcaaacgcc aattggttga aactcgccaa atcactaagc atgtggcaca aattttggat
     3601 agtcgcatga atactaaata cgatgaaaat gataaactta ttcgagaggt taaagtgatt
     3661 accttaaaat ctaaattagt ttctgacttc cgaaaagatt tccaattcta taaagtacgt
     3721 gagattaaca attaccatca tgcccatgat gcgtatctaa atgccgtcgt tggaactgct
     3781 ttgattaaga aatatccaaa acttgaatcg gagtttgtct atggtgatta taaagtttat
     3841 gatgttcgta aaatgattgc taagtctgag caagaaatag gcaaagcaac cgcaaaatat
     3901 ttcttttact ctaatatcat gaacttcttc aaaacagaaa ttacacttgc aaatggagag
     3961 attcgcaaac gccctctaat cgaaactaat ggggaaactg gagaaattgt ctgggataaa
     4021 gggcgagatt ttgccacagt gcgcaaagta ttgtccatgc cccaagtcaa tattgtcaag
     4081 aaaacagaag tacagacagg cggattctcc aaggagtcaa ttttaccaaa aagaaattcg
     4141 gacaagctta ttgctcgtaa aaaagactgg gatccaaaaa aatatggtgg ttttgatagt
     4201 ccaacggtag cttattcagt cctagtggtt gctaaggtgg aaaaagggaa atcgaagaag
     4261 ttaaaatccg ttaaagagtt actagggatc acaattatgg aaagaagttc ctttgaaaaa
     4321 aatccgattg actttttaga agctaaagga tataaggaag ttaaaaaaga cttaatcatt
     4381 aaactaccta aatatagtct ttttgagtta gaaaacggtc gtaaacggat gctggctagt
     4441 gccggagaat tacaaaaagg aaatgagctg gctctgccaa gcaaatatgt gaatttttta
     4501 tatttagcta gtcattatga aaagttgaag ggtagtccag aagataacga acaaaaacaa
     4561 ttgtttgtgg agcagcataa gcattattta gatgagatta ttgagcaaat cagtgaattt
     4621 tctaagcgtg ttattttagc agatgccaat ttagataaag ttcttagtgc atataacaaa
     4681 catagagaca aaccaatacg tgaacaagca gaaaatatta ttcatttatt tacgttgacg
     4741 aatcttggag ctcccgctgc ttttaaatat tttgatacaa caattgatcg taaacgatat
     4801 acgtctacaa aagaagtttt agatgccact cttatccatc aatccatcac tggtctttat
     4861 gaaacacgca ttgatttgag tcagctagga ggtgactaat actagagcca attattgaag
     4921 gggagcggga aaccgctccc ctttttttgt ttctggtctc ccctctagaa gcggccgcga
     4981 attccagaaa tcatccttag cgaaagctaa ggattttttt tatctgaaat tctgcctcgt
     5041 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
     5101 caccatggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     5161 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     5221 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     5281 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     5341 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     5401 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     5461 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     5521 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     5581 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     5641 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     5701 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     5761 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     5821 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     5881 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     5941 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     6001 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     6061 ctcactgatt aagcattggt aactgtcaga ccaagtttac gagctcgctt ggactcctgt
     6121 tgatagatcc agtaatgacc tcagaactcc atctggattt gttcagaacg ctcggttgcc
     6181 gccgggcgtt ttttattggt gagaatccaa gcactaggga cagtaagacg ggtaagcctg
     6241 ttgatgatac cgctgcctta ctgggtgcat tagccagtct gaatgacctg tcacgggata
     6301 atccgaagtg gtcagactgg aaaatcagag ggcaggaact gctgaacagc aaaaagtcag
     6361 atagcaccac atagcagacc cgccataaaa cgccctgaga agcccgtgac gggcttttct
     6421 tgtattatgg gtagtttcct tgcatgaatc cataaaaggc gcctgtagtg ccatttaccc
     6481 ccattcactg ccagagccgt gagcgcagcg aactgaatgt cacgaaaaag acagcgactc
     6541 aggtgcctga tggtcggaga caaaaggaat attcagcgat ttgcccgagc ttgcgagggt
     6601 gctacttaag cctttagggt tttaaggtct gttttgtaga ggagcaaaca gcgtttgcga
     6661 catccttttg taatactgcg gaactgacta aagtagtgag ttatacacag ggctgggatc
     6721 tattcttttt atcttttttt attctttctt tattctataa attataacca cttgaatata
     6781 aacaaaaaaa acacacaaag gtctagcgga atttacagag ggtctagcag aatttacaag
     6841 ttttccagca aaggtctagc agaatttaca gatacccaca actcaaagga aaaggacatg
     6901 taattatcat tgactagccc atctcaattg gtatagtgat taaaatcacc tagaccaatt
     6961 gagatgtatg tctgaattag ttgttttcaa agcaaatgaa ctagcgatta gtcgctatga
     7021 cttaacggag catgaaacca agctaatttt atgctgtgtg gcactactca accccacgat
     7081 tgaaaaccct acaaggaaag aacggacggt atcgttcact tataaccaat acgctcagat
     7141 gatgaacatc agtagggaaa atgcttatgg tgtattagct aaagcaacca gagagctgat
     7201 gacgagaact gtggaaatca ggaatccttt ggttaaaggc tttgagattt tccagtggac
     7261 aaactatgcc aagttctcaa gcgaaaaatt agaattagtt tttagtgaag agatattgcc
     7321 ttatcttttc cagttaaaaa aattcataaa atataatctg gaacatgtta agtcttttga
     7381 aaacaaatac tctatgagga tttatgagtg gttattaaaa gaactaacac aaaagaaaac
     7441 tcacaaggca aatatagaga ttagccttga tgaatttaag ttcatgttaa tgcttgaaaa
     7501 taactaccat gagtttaaaa ggcttaacca atgggttttg aaaccaataa gtaaagattt
     7561 aaacacttac agcaatatga aattggtggt tgataagcga ggccgcccga ctgatacgtt
     7621 gattttccaa gttgaactag atagacaaat ggatctcgta accgaacttg agaacaacca
     7681 gataaaaatg aatggtgaca aaataccaac aaccattaca tcagattcct acctacataa
     7741 cggactaaga aaaacactac acgatgcttt aactgcaaaa attcagctca ccagttttga
     7801 ggcaaaattt ttgagtgaca tgcaaagtaa gtatgatctc aatggttcgt tctcatggct
     7861 cacgcaaaaa caacgaacca cactagagaa catactggct aaatacggaa ggatctgagg
     7921 ttcttatggc tcttgtatct atcagtgaag catcaagact aacaaacaaa agtagaacaa
     7981 ctgttcaccg ttacatatca aagggaaaac tgtccatatg cacagatgaa aacggtgtaa
     8041 aaaagataga tacatcagag cttttacgag tttttggtgc attcaaagct gttcaccatg
     8101 aacagatcga caatgtaacc tcgagattac cgcctttgag tgagctgata ccgctcgccg
     8161 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcctgcataa cgcgaagtaa
     8221 tcttttcggt tttaaagaaa aagggcaggg tggtgacacc ttgccctttt ttgccggact
     8281 gcagcggccg ctactagtag ggagaccaga aacaaaaaaa ggccccccgt tagggaggcc
     8341 ttcaataatt ggctctagta ttattaaact gatgcagcgt agttttcgtc gtttgcagca
     8401 ggccttttgt acagttcatc cataccatgc gtgatgcccg ctgcggttac gaactccagc
     8461 agaaccatat gatcgcgttt ctcgttcgga tctttagaca gaacgctttg cgtgctcaga
     8521 tagtgattgt ctggcagcag aacaggacca tcaccgattg gagtgttttg ctggtagtga
     8581 tcagccagct gcacgctgcc atcctccacg ttgtggcgaa ttttaaaatt cgctttaatg
     8641 ccattttttt gtttatcggc ggtgatgtaa acattgtggc tgttaaaatt gtattccagc
     8701 ttatggccca ggatattgcc gtcttcttta aagtcaatgc ctttcagctc aatgcggttt
     8761 accagggtat cgccttcaaa tttcacttcc gcacgcgttt tgtacgtgcc gtcatcctta
     8821 aaggaaatcg tgcgttcctg cacatagcct tccggcatgg cggacttgaa gaagtcatgc
     8881 tgcttcatat ggtccggata acgagcaaag cactgaacac cataagtcag cgtcgttacc
     8941 agagtcggcc aaggtaccgg cagtttacca gtagtacaga tgaacttcag cgtcagttta
     9001 ccattagttg cgtcaccttc accctcgcca cgcacggaaa acttatgacc gttgacatca
     9061 ccatccagtt ccaccagaat agggacgaca ccagtgaaca gctcttcgcc tttacgcata
     9121 tggtatttct cctctttaat ctctagttaa acaaaattat ttgtagaggc tgtttcgtcc
     9181 tcacggactc atcagaccgg aaagcacatc cggtgacagc tctctagtat ttgcgttcag
     9241 gattgtcctg ctgaactgtt aatacaattt gcgtgccaat tttttatctt tttgatttat
     9301 aaagatctga ttgaagaatc aacagcaaca tgccaggatg agttagcgaa ttacactaac
     9361 cgtcacgttg gctttagtta ttctctagta gggagaccag aaacaaaaaa aggccgcgtt
     9421 agcggccttc aataattggc tctagtatta atttgctgct ttccattgag cctgtttctc
     9481 tgcgcgacgt tcgcggcggc gtgtgcgtgc attcatttgc aaactgagtt gcagcaactc
     9541 tttttcctgc tgcatctgaa actcacgtaa atccagcggc agtgttggaa gcgaggtggt
     9601 ttctgaaacg gcgatagcgt cttcaggcgg acgccgttta aagggatcaa taatgatgtc
     9661 atcaagcgga taatcgctgg tgccgtggcg atacactgaa cgttccacca cgtttttcaa
     9721 ttcacgaata tttcccggcc aacgataatt cagcaatgtt tctctggcgc gctccgtaaa
     9781 ccccgggaac agaggcagct tgatttcccg acacatctgg atggcaaagt attctgccat
     9841 caacattatg tcgctttcgc gctcgcgcag tggtggcagt tgtacaacat caaaagccag
     9901 tcggtcgagc aggtcagcgc gaaaagtgcc ttcattgacc atcgccggga gatcggcatt
     9961 cgtcgcgcat accaaccgca cattcacctg caatggttgg ctgccgccaa cgcgctccag
    10021 ttcaccgtac tcaatcacgc gcaataattt ctcctgcacc atcatcggtg ccgtagcgag
    10081 ttcatcaaga aatagcgtac cgccgtcggc acgttcaaat ctccctggat gacgtttttg
    10141 cgcaccggta aacgcccccg cttcgtgacc aaacagttcg gaatccagca gattttcatt
    10201 taacgccgcg cagttaaggg aaataaacgg cccttgccaa cgggaggaga gataatgcag
    10261 gcggctggca atcagctctt taccggtgcc gcgttcgccg atgatgagca ccggtttgtc
    10321 cagcggtgcg agatgcgaaa cctgttccag cacttcgaga aagctgttcg cctcaccaag
    10381 taaattatct ttgtattctg ccatctagta ctttcctgtg tgactctagt tacgaccagt
    10441 ctaaaaagcg cctcaattcg cgaccttctc gttactgaca ggaaaatggg ccattggcaa
    10501 ccagggaaag atgaacgtga tgatgttcac aatttgctga attgtggtga tgtgatgctc
    10561 accgcattcc tgctccagcg gcgtaaaggt ctctctagta tataaacgca gaaaggccca
    10621 cccgaaggtg agccagtgtg atactagagt ttacagctag ctcagtccta ggtattatgc
    10681 tagctactag agggagccca aatgaccgta ttacatagtg tggatttttt tccgtccggt
    10741 aacgcgtccg tggcgataga accccggctc ccgcaggcgg attttcctga acatcatcat
    10801 gattttcatg aaattgtgat tgtcgaacat ggcacgggta ttcatgtgtt taatgggcag
    10861 ccctatacca tcaccggtgg cacggtctgt ttcgtacgcg atcatgatcg gcatctgtat
    10921 gaacataccg ataatctgtg tctgaccaat gtgctgtatc gctcgccgga tcgatttcag
    10981 tttctcgccg ggctgaatca gttgctgcca caagagctgg atgggcagta tccgtctcac
    11041 tggcgcgtta accacagcgt attgcagcag gtgcgacagc tggttgcaca gatggaacag
    11101 caggaagggg aaaatgattt accctcgacc gccagtcgcg agatcttgtt tatgcaatta
    11161 ctgctcttgc tgcgtaaaag cagtttgcag gagaacctgg aaaacagcgc atcacgtctc
    11221 aacttgcttc tggcctggct ggaggaccat tttgccgatg aggtgaattg ggatgccgtg
    11281 gcggatcaat tttctctttc actgcgtacg ctacatcggc agcttaagca gcaaacggga
    11341 ctgacgcctc agcgatacct gaaccgcctg cgactgatga aagcccgaca tctgctacgc
    11401 cacagcgagg ccagcgttac tgacatcgcc tatcgctgtg gattcagcga cagtaaccac
    11461 ttttcgacgc tttttcgccg agagtttaac tggtcaccgc gtgatattcg ccagggacgg
    11521 gatggctttc tgcaataata ctagctcggt accaaagacg aacaataaga cgctgaaaag
    11581 cgtctttttt cgttttggtc cctctagtat taattttgtc ttcatgcatg aagacaacta
    11641 ggctctag
//
