LOCUS       sequence-376085-        8704 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For knocking p2a-tdTomato into CLSTN2 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8704)
  AUTHORS   Xu P, He H, Gao Q, Zhou Y, Wu Z, Zhang X, Sun L, Hu G, Guan Q, You 
            Z, Zhang X, Zheng W, Xiong M, Chen Y
  TITLE     Human midbrain dopaminergic neuronal differentiation markers predict
            cell therapy outcomes in a Parkinson's disease model.
  JOURNAL   J Clin Invest. 2022 Jul 15;132(14). pii: 156768. doi: 
            10.1172/JCI156768.
  PUBMED    35700056
REFERENCE   2  (bases 1 to 8704)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Clin 
            Invest."; date: "2022-07-15"; volume: "132(14). pii: 156768. doi"; 
            pages: " 10.1172/JCI156768"
COMMENT     Sequence Label: 
            PL752-CLSTN2-LA-p2a-tdTomato-hGHpA-PGK-puroR-CLSTN2-RA
FEATURES             Location/Qualifiers
     source          1..8704
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(16..444)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(126..147)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     338..357
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        471..575
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(576..644,645..1436)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(794..813)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1607..2195
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2096..2115
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2349..2366
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2483..2504
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2519..2524,2525..2542,2543..2549)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2557..2573
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2562..2584
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2581..2597
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2581..2597
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        2618..2636
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             3669..3725
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3726..5156
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     polyA_signal    5221..5697
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(5420..5439)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     promoter        5758..6245
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5793..5816)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6178..6197
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6285..6884
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APHNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6285..6304)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     complement(8510..8529)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8511..8529)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8536..8553)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8536..8552)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8545..8567)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat
       61 aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg
      121 ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc
      181 gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt
      241 tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag
      301 cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg
      361 gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc
      421 ttaatgcgcc gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
      481 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
      541 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
      601 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
      661 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
      721 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
      781 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
      841 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
      901 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
      961 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     1021 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     1081 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     1141 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     1201 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     1261 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     1321 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     1381 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     1441 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     1501 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     1561 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     1621 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     1681 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     1741 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     1801 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     1861 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     1921 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     1981 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     2041 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     2101 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     2161 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     2221 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     2281 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     2341 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     2401 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     2461 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     2521 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     2581 caggaaacag ctatgaccat gattacgcca agctcgaaat taaccctcac taaagggaac
     2641 aaaagctggt accttggagg aggttggcct aggggagacc ttgaagagtg ggtaggattt
     2701 ggggtaaggt agaaggtagg aggtaaaaga caattcctaa gaagtgtaag gggaggggtt
     2761 gggtagccaa gcaaggagaa acaactttta tgacctggtt catcaatgca gaacataaat
     2821 ccagaaatga aattttgctg gaattatcca aaagctggag ggagggggaa ttggactgga
     2881 aaggaagggg tacagcctga ggacacaagt cagggggtga gcagcaagca tggaaagtga
     2941 ggaccaataa atttgtcctt tccaaaggaa tatttttgag gggaagcata cctgagtctt
     3001 tgcttagaat gccttctgct cccagggaca ttgcatgcac acttggaaaa gagacacaag
     3061 atcaaatgtc atcctctcac tgagaagcaa acactaaggc ttggacagct ccatttaaat
     3121 aagctccaga aatgcctgaa aacaagcctt ggcctgaaag gttcagtaaa tgatggatag
     3181 gattggctaa aatgtccagc ccatcattta tacccaacag tctaccttga gttcatgcct
     3241 tggaatttgc tatttcaggc ttttgccttg aaccttctag cttccttttt accagaagac
     3301 ttcccttcca ggaagtctgt ctccaggctg aagagttttg cactaaaaga agaaattcca
     3361 aggggccgta aatgtttcct taatggatgg agagacataa gctccaaaat ggcctctgtt
     3421 cagcccctga tgagcatttg ctttttctcc ttgatatcca gaaacatgaa ggaccagggc
     3481 atggggaaga tgagactgag ggagaagagg aggaagaagc cgaggaagaa atgagctcca
     3541 gcagtggctc tgacgacagc gaagaggagg aggaggagga agggatgggc agaggcagac
     3601 atgggcagaa tggagccagg caagcccagc tggagtggga tgactccacc ctcccctacg
     3661 gatccggcgc aacaaacttc tctctgctga aacaagccgg agatgtcgaa gagaatcctg
     3721 gaccgatggt gagcaagggc gaggaggtca tcaaagagtt catgcgcttc aaggtgcgca
     3781 tggagggctc catgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct
     3841 acgagggcac ccagaccgcc aagctgaagg tgaccaaggg cggccccctg cccttcgcct
     3901 gggacatcct gtccccccag ttcatgtacg gctccaaggc gtacgtgaag caccccgccg
     3961 acatccccga ttacaagaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga
     4021 acttcgagga cggcggtctg gtgaccgtga cccaggactc ctccctgcag gacggcacgc
     4081 tgatctacaa ggtgaagatg cgcggcacca acttcccccc cgacggcccc gtaatgcaga
     4141 agaagaccat gggctgggag gcctccaccg agcgcctgta cccccgcgac ggcgtgctga
     4201 agggcgagat ccaccaggcc ctgaagctga aggacggcgg ccactacctg gtggagttca
     4261 agaccatcta catggccaag aagcccgtgc aactgcccgg ctactactac gtggacacca
     4321 agctggacat cacctcccac aacgaggact acaccatcgt ggaacagtac gagcgctccg
     4381 agggccgcca ccacctgttc ctggggcatg gcaccggcag caccggcagc ggcagctccg
     4441 gcaccgcctc ctccgaggac aacaacatgg ccgtcatcaa agagttcatg cgcttcaagg
     4501 tgcgcatgga gggctccatg aacggccacg agttcgagat cgagggcgag ggcgagggcc
     4561 gcccctacga gggcacccag accgccaagc tgaaggtgac caagggcggc cccctgccct
     4621 tcgcctggga catcctgtcc ccccagttca tgtacggctc caaggcgtac gtgaagcacc
     4681 ccgccgacat ccccgattac aagaagctgt ccttccccga gggcttcaag tgggagcgcg
     4741 tgatgaactt cgaggacggc ggtctggtga ccgtgaccca ggactcctcc ctgcaggacg
     4801 gcacgctgat ctacaaggtg aagatgcgcg gcaccaactt cccccccgac ggccccgtaa
     4861 tgcagaagaa gaccatgggc tgggaggcct ccaccgagcg cctgtacccc cgcgacggcg
     4921 tgctgaaggg cgagatccac caggccctga agctgaagga cggcggccac tacctggtgg
     4981 agttcaagac catctacatg gccaagaagc ccgtgcaact gcccggctac tactacgtgg
     5041 acaccaagct ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgagc
     5101 gctccgaggg ccgccaccac ctgttcctgt acggcatgga cgagctgtac aagtaatgag
     5161 aattctcaat ttctgagaac tgtcattctc ggaaattgaa gcgctgctcg agagatctac
     5221 gggtggcatc cctgtgaccc ctccccagtg cctctcctgg ccctggaagt tgccactcca
     5281 gtgcccacca gccttgtcct aataaaatta agttgcatca ttttgtctga ctaggtgtcc
     5341 ttctataata ttatggggtg gaggggggtg gtatggagca aggggcaagt tgggaagaca
     5401 acctgtaggg cctgcggggt ctattgggaa ccaagctgga gtgcagtggc acaatcttgg
     5461 ctcactgcaa tctccgcctc ctgggttcaa gcgattctcc tgcctcagcc tcccgagttg
     5521 ttgggattcc aggcatgcat gaccaggctc agctaatttt tgtttttttg gtagagacgg
     5581 ggtttcacca tattggccag gctggtctcc aactcctaat ctcaggtgat ctacccacct
     5641 tggcctccca aattgctggg attacaggcg tgaaccactg ctcccttccc tgtcctttag
     5701 gtctgaagag gagtttacgt ccagccacgc gtaggatctc gacctcgaaa ttctaccggg
     5761 taggggaggc gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca
     5821 cttggcgcta cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc
     5881 aaccgactcc gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga
     5941 agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc
     6001 tcactagtct cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg
     6061 gcagcggcca atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg
     6121 ggtccggggg cgggctcagg ggcggggcgg gcgcccgaag gtcctccgga ggcccggcat
     6181 tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc ctcttcctca tctccgggcc
     6241 tttcgacctg catccatcta gatctcgagc agctgaagct taccatgacc gagtacaagc
     6301 ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg
     6361 cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg
     6421 tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg
     6481 tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg
     6541 cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc
     6601 agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg
     6661 ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc
     6721 ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc
     6781 acaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg
     6841 aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgacgcccg ccccacgacc
     6901 cgcagcgccc gaccgaaagg agcgcacgac cccatgcatc gatgatatca gatccccggg
     6961 atgcagaaat tgatgatcta ttaaacaata aagatgtcca ctaaaatgga agtttttcct
     7021 gtcatacttt gttaagaagg gtgagaacag agtacctaca ttttgaatgg aaggattgga
     7081 gctacggggg tgggggtggg gtgggattag ataaatgcct gctctttact gaaggctctt
     7141 tactattgct ttatgataat gtttcatagt tggatatcat aatttaaaca agcaaaacca
     7201 aattaagggc cagctcattc ctcccactca tgatctatag atctatagat ctctcgtggg
     7261 atcattgttt ttctcttgat tcccactttg tggttctaag tactgtggtt tccaaatgtg
     7321 tcagtttcat agcctgaaga acgagatcag cagcctctgt tccacataca cttcattctc
     7381 agtattgttt tgccaagttc taattccatc agaagctggt cgagatccgg aacccttaat
     7441 tcaatttctg agaactgtca ttctcggaaa ttgaggatcc tagtgcccag gggtctgctg
     7501 cctggcccac atgtcccttt tgtaaaccct gacccaacgt atgcccgcgt ctatcatacc
     7561 tcacctctga tgtctgtgac atgtctggac aggccttctc cagcttcctg gagcccaccc
     7621 tttaagcctt gggcactccc tgtgtttcat ccatggggaa gttccaagaa gcccagcatg
     7681 gccatcagtg aggacttcag ggtagacttt gtcctgtagc ctccacttct gccctaagtt
     7741 ccccagcatc ctgactacct gtctgcagag tttgcctttg ttttttcctg cagggaagaa
     7801 ggcccacctt tgtgtcactc acctccccag gctcagagtc cccaaggccc tggggttcca
     7861 actcactgtg cgtctcctcc acacagacca gtaggttctc ctatgctgac tccaggttgc
     7921 ttcatacaag gagggtggtt gaacttcaca cacgtaaggt cttagtgctt aacagtttaa
     7981 aggaaagtcc ttgttgaggc agaactaagt ttacagggaa aggtacacac attctctctc
     8041 tctctctctc tgtctatcta gttccccagc ttggagagcc tttccccttg cttctttctg
     8101 aggccatata agcttataag aaaagtccca aaccaagaat aggtccttgg ccacaagcag
     8161 ggtctgatcc cccatcagag ctatctgagc ctgcctgtct gggcacctgc tgcaaccatg
     8221 cagctaccct gccaggggca ctcagcaaac agaaccacag ggcccaggag gcattccaca
     8281 caggcactgc cccaggacaa cacaacaagg acagtcacaa caaggacaac aaggacacaa
     8341 cacaacacac aacaaggaca gtcacaacaa gcctagagcc agaaagcaga tggaaatgct
     8401 aatgaggtca aacgtaggct tcatggtggg tggagtgggg gtggctgggc tcccccagga
     8461 cagaggggac cctgaggtgc ggccgccacc gcggtggagc tccaattcgc cctatagtga
     8521 gtcgtattac aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
     8581 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     8641 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggaaattgta
     8701 agcg
//
