LOCUS       sequence-376093-        8041 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For knocking p2a-EFGP into CLSTN2 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8041)
  AUTHORS   Xu P, He H, Gao Q, Zhou Y, Wu Z, Zhang X, Sun L, Hu G, Guan Q, You 
            Z, Zhang X, Zheng W, Xiong M, Chen Y
  TITLE     Human midbrain dopaminergic neuronal differentiation markers predict
            cell therapy outcomes in a Parkinson's disease model.
  JOURNAL   J Clin Invest. 2022 Jul 15;132(14). pii: 156768. doi: 
            10.1172/JCI156768.
  PUBMED    35700056
REFERENCE   2  (bases 1 to 8041)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Clin 
            Invest."; date: "2022-07-15"; volume: "132(14). pii: 156768. doi"; 
            pages: " 10.1172/JCI156768"
COMMENT     Sequence Label: PL452-CLSTN2-LA-p2a-EGFP-hGHpA-PGK-neoR-CLSTN2-RA
FEATURES             Location/Qualifiers
     source          1..8041
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1016..1072
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(1073..1075,1076..1078,1079..1792)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1073..1075,1076..1078,1079..1789)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1118..1139)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1379..1398)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1726..1747
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    1826..1859
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    1881..2357
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(2080..2099)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     promoter        2418..2917
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2453..2476)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     2850..2869
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     promoter        2929..2976
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             2995..3798
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3058..3077)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3668..3687
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(3830..3847)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3836..4060
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    4117..4150
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(5194..5213)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5195..5213)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5220..5237)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5220..5236)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5229..5251)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(5377..5832)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5514..5535)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5726..5745
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        5859..5963
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5964..6032,6033..6824)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6182..6201)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6995..7583
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7484..7503
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7737..7754
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7871..7892
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7907..7912,7913..7930,7931..7937)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7945..7961
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7950..7972
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7969..7985
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7969..7985
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        8006..8024
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttggaggagg ttggcctagg ggagaccttg aagagtgggt aggatttggg gtaaggtaga
       61 aggtaggagg taaaagacaa ttcctaagaa gtgtaagggg aggggttggg tagccaagca
      121 aggagaaaca acttttatga cctggttcat caatgcagaa cataaatcca gaaatgaaat
      181 tttgctggaa ttatccaaaa gctggaggga gggggaattg gactggaaag gaaggggtac
      241 agcctgagga cacaagtcag ggggtgagca gcaagcatgg aaagtgagga ccaataaatt
      301 tgtcctttcc aaaggaatat ttttgagggg aagcatacct gagtctttgc ttagaatgcc
      361 ttctgctccc agggacattg catgcacact tggaaaagag acacaagatc aaatgtcatc
      421 ctctcactga gaagcaaaca ctaaggcttg gacagctcca tttaaataag ctccagaaat
      481 gcctgaaaac aagccttggc ctgaaaggtt cagtaaatga tggataggat tggctaaaat
      541 gtccagccca tcatttatac ccaacagtct accttgagtt catgccttgg aatttgctat
      601 ttcaggcttt tgccttgaac cttctagctt cctttttacc agaagacttc ccttccagga
      661 agtctgtctc caggctgaag agttttgcac taaaagaaga aattccaagg ggccgtaaat
      721 gtttccttaa tggatggaga gacataagct ccaaaatggc ctctgttcag cccctgatga
      781 gcatttgctt tttctccttg atatccagaa acatgaagga ccagggcatg gggaagatga
      841 gactgaggga gaagaggagg aagaagccga ggaagaaatg agctccagca gtggctctga
      901 cgacagcgaa gaggaggagg aggaggaagg gatgggcaga ggcagacatg ggcagaatgg
      961 agccaggcaa gcccagctgg agtgggatga ctccaccctc ccctacggat ccggcgcaac
     1021 aaacttctct ctgctgaaac aagccggaga tgtcgaagag aatcctggac cgatggtgag
     1081 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
     1141 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct
     1201 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
     1261 caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga
     1321 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga
     1381 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg
     1441 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga
     1501 gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa
     1561 ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta
     1621 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
     1681 cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga
     1741 gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt aatgagaatt
     1801 ccgatcatat tcaataaccc ttaatataac ttcgtataat gtatgctata cgaagttata
     1861 gcgctgctcg agagatctac gggtggcatc cctgtgaccc ctccccagtg cctctcctgg
     1921 ccctggaagt tgccactcca gtgcccacca gccttgtcct aataaaatta agttgcatca
     1981 ttttgtctga ctaggtgtcc ttctataata ttatggggtg gaggggggtg gtatggagca
     2041 aggggcaagt tgggaagaca acctgtaggg cctgcggggt ctattgggaa ccaagctgga
     2101 gtgcagtggc acaatcttgg ctcactgcaa tctccgcctc ctgggttcaa gcgattctcc
     2161 tgcctcagcc tcccgagttg ttgggattcc aggcatgcat gaccaggctc agctaatttt
     2221 tgtttttttg gtagagacgg ggtttcacca tattggccag gctggtctcc aactcctaat
     2281 ctcaggtgat ctacccacct tggcctccca aattgctggg attacaggcg tgaaccactg
     2341 ctcccttccc tgtcctttag gtctgaagag gagtttacgt ccagccacgc gtaggatctc
     2401 gacctcgaaa ttctaccggg taggggaggc gcttttccca aggcagtctg gagcatgcgc
     2461 tttagcagcc ccgctgggca cttggcgcta cacaagtggc ctctggcctc gcacacattc
     2521 cacatccacc ggtaggcgcc aaccggctcc gttctttggt ggccccttcg cgccaccttc
     2581 tactcctccc ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg
     2641 acaaatggaa gtagcacgtc tcactagtct cgtgcagatg gacagcaccg ctgagcaatg
     2701 gaagcgggta ggcctttggg gcagcggcca atagcagctt tgctccttcg ctttctgggc
     2761 tcagaggctg ggaaggggtg ggtccggggg cgggctcagg ggcgggctca ggggcggggc
     2821 gggcgcccga aggtcctccg gaggcccggc attctgcacg cttcaaaagc gcacgtctgc
     2881 cgcgctgttc tcctcttcct catctccggg cctttcgacc tgcagcctgt tgacaattaa
     2941 tcatcggcat agtatatcgg catagtataa tacgacaagg tgaggaacta aaccatggga
     3001 tcggccattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta
     3061 ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg
     3121 tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa
     3181 ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct
     3241 gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg
     3301 caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca
     3361 atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat
     3421 cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac
     3481 gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc
     3541 gacggcgatg atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa
     3601 aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag
     3661 gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc
     3721 ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt
     3781 cttgacgagt tcttctgagg ggatcaattc tctagagctc gctgatcagc ctcgactgtg
     3841 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa
     3901 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt
     3961 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa
     4021 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc
     4081 agctggggct cgactagagc ttgcggaacc cttaatataa cttcgtataa tgtatgctat
     4141 acgaagttat taggtccctc gaggtagtgc ccaggggtct gctgcctggc ccacatgtcc
     4201 cttttgtaaa ccctgaccca acgtatgccc gcgtctatca tacctcacct ctgatgtctg
     4261 tgacatgtct ggacaggcct tctccagctt cctggagccc accctttaag ccttgggcac
     4321 tccctgtgtt tcatccatgg ggaagttcca agaagcccag catggccatc agtgaggact
     4381 tcagggtaga ctttgtcctg tagcctccac ttctgcccta agttccccag catcctgact
     4441 acctgtctgc agagtttgcc tttgtttttt cctgcaggga agaaggccca cctttgtgtc
     4501 actcacctcc ccaggctcag agtccccaag gccctggggt tccaactcac tgtgcgtctc
     4561 ctccacacag accagtaggt tctcctatgc tgactccagg ttgcttcata caaggagggt
     4621 ggttgaactt cacacacgta aggtcttagt gcttaacagt ttaaaggaaa gtccttgttg
     4681 aggcagaact aagtttacag ggaaaggtac acacattctc tctctctctc tctctgtcta
     4741 tctagttccc cagcttggag agcctttccc cttgcttctt tctgaggcca tataagctta
     4801 taagaaaagt cccaaaccaa gaataggtcc ttggccacaa gcagggtctg atcccccatc
     4861 agagctatct gagcctgcct gtctgggcac ctgctgcaac catgcagcta ccctgccagg
     4921 ggcactcagc aaacagaacc acagggccca ggaggcattc cacacaggca ctgccccagg
     4981 acaacacaac aaggacagtc acaacaagga caacaaggac acaacacaac acacaacaag
     5041 gacagtcaca acaagcctag agccagaaag cagatggaaa tgctaatgag gtcaaacgta
     5101 ggcttcatgg tgggtggagt gggggtggct gggctccccc aggacagagg ggaccctgag
     5161 gtgcggccgc caccgcggtg gagctccaat tcgccctata gtgagtcgta ttacaattca
     5221 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
     5281 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
     5341 ccttcccaac agttgcgcag cctgaatggc gaatggaaat tgtaagcgtt aatattttgt
     5401 taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg
     5461 gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt
     5521 ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct
     5581 atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt
     5641 gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa
     5701 agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc
     5761 tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc
     5821 tacagggcgc gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     5881 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     5941 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     6001 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     6061 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     6121 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     6181 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     6241 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     6301 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     6361 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     6421 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     6481 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     6541 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     6601 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     6661 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     6721 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     6781 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     6841 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     6901 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     6961 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     7021 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     7081 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     7141 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     7201 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     7261 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     7321 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     7381 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     7441 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     7501 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     7561 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     7621 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     7681 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     7741 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
     7801 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
     7861 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
     7921 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
     7981 atgaccatga ttacgccaag ctcgaaatta accctcacta aagggaacaa aagctggtac
     8041 c
//
