LOCUS       sequence-376098-       11885 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Retroviral transgene expression of human ZNF335.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11885)
  AUTHORS   Funk L, Su KC, Ly J, Feldman D, Singh A, Moodie B, Blainey PC, 
            Cheeseman IM
  TITLE     The phenotypic landscape of essential human genes.
  JOURNAL   Cell. 2022 Nov 23;185(24):4634-4653.e22. doi: 
            10.1016/j.cell.2022.10.017. Epub 2022 Nov 7.
  PUBMED    36347254
REFERENCE   2  (bases 1 to 11885)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.10.017"; journalName: "Cell"; date: 
            "2022-11-23- 23"; volume: "185"; issue: "24"; pages: "4634-4653.e22"
COMMENT     Sequence Label: pKC530 pBABE EGFP-ZNF335
FEATURES             Location/Qualifiers
     source          1..11885
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..22
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(37..42,43..60,61..67)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    75..91
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     80..102
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     99..115
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     99..115
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     134..154
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        136..154
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        182..408
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             409..589
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    636..761
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1132..1365
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    1416..1533
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1564..2775
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     2612..2631
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      2782..2791
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2788..2790,2791..2793,2794..3504)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2833..2854)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3094..3113)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3441..3462
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             3550..3570
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             3583..3627
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     primer_bind     complement(7735..7752)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     promoter        7759..8269
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     8188..8206
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             8277..8876
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8277..8296)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8773..8793
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8885..9473
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8938..8958)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9356..9367)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9567..9800
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9872..9993
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9909..9928)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9963..9982
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     10095..10114
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        10143..10247
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10248..10316,10317..11108)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10466..10485)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11279..11867
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11768..11787
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
       61 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
      121 ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagctta
      181 atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag ttagcaacat
      241 gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat
      301 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg
      361 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg
      421 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
      481 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
      541 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
      601 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
      661 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
      721 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
      781 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
      841 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
      901 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
      961 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     1021 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     1081 agaccaccgc acagcaagcg gccgacagag agaaaaaaga gcagtgggaa taggagcttt
     1141 gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac
     1201 ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc
     1261 tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc
     1321 aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg
     1381 ctctggaaaa ctcatttgca ccactgtaac ctagctttta aaagaaaagg ggggattggg
     1441 gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa
     1501 ttacaaaaac aaattacaaa aattcaaaat tttcctaaaa cgctgtcgag caattgcaga
     1561 tctggcctcc gcgccgggtt ttggcgcctc ccgcgggcgc ccccctcctc acggcgagcg
     1621 ctgccacgtc agacgaaggg cgcagcgagc gtcctgatcc ttccgcccgg acgctcagga
     1681 cagcggcccg ctgctcataa gactcggcct tagaacccca gtatcagcag aaggacattt
     1741 taggacggga cttgggtgac tctagggcac tggttttctt tccagagagc ggaacaggcg
     1801 aggaaaagta gtcccttctc ggcgattctg cggagggatc tccgtggggc ggtgaacgcc
     1861 gatgattata taaggacgcg ccgggtgtgg cacagctagt tccgtcgcag ccgggatttg
     1921 ggtcgcggtt cttgtttgtg gatcgctgtg atcgtcactt ggtgagtagc gggctgctgg
     1981 gctggccggg gctttcgtgg ccgccgggcc gctcggtggg acggaagcgt gtggagagac
     2041 cgccaagggc tgtagtctgg gtccgcgagc aaggttgccc tgaactgggg gttgggggga
     2101 gcgcagcaaa atggcggctg ttcccgagtc ttgaatggaa gacgcttgtg aggcgggctg
     2161 tgaggtcgtt gaaacaaggt ggggggcatg gtgggcggca agaacccaag gtcttgaggc
     2221 cttcgctaat gcgggaaagc tcttattcgg gtgagatggg ctggggcacc atctggggac
     2281 cctgacgtga agtttgtcac tgactggaga actcggtttg tcgtctgttg cgggggcggc
     2341 agttatggcg gtgccgttgg gcagtgcacc cgtacctttg ggagcgcgcg ccctcgtcgt
     2401 gtcgtgacgt cacccgttct gttggcttat aatgcagggt ggggccacct gccggtaggt
     2461 gtgcggtagg cttttctccg tcgcaggacg cagggttcgg gcctagggta ggctctcctg
     2521 aatcgacagg cgccggacct ctggtgaggg gagggataag tgaggcgtca gtttctttgg
     2581 tcggttttat gtacctatct tcttaagtag ctgaagctcc ggttttgaac tatgcgctcg
     2641 gggttggcga gtgtgttttg tgaagttttt taggcacctt ttgaaatgta atcatttggg
     2701 tcaatatgta attttcagtg ttagactagt aaattgtccg ctaaattctg gccgtttttg
     2761 gcttttttgt tagacgtcga cgccaccatg gtgagcaagg gcgaggagct gttcaccggg
     2821 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
     2881 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
     2941 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc
     3001 ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
     3061 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
     3121 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
     3181 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc
     3241 tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac
     3301 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
     3361 ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccaagct gagcaaagac
     3421 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
     3481 ctcggcatgg acgagctgta caagtccgga ggggatcggt ggagctccac cggtggcggc
     3541 cgctctagag agaatcttta ttttcagggc gccgccaaat tcaaagaaac cgctgctgct
     3601 aaattcgaac gccagcacat ggacagcgga ggtggaggtc tcgacatgga ggagaacgag
     3661 gtggagagca gcagcgacgc ggcccctggg cctggccggc ccgaggagcc ctctgagagc
     3721 ggcctgggtg tgggcacctc agaagccgtg tccgccgaca gcagcgacgc cgcggccgcc
     3781 ccggggcagg cagaggccga tgactctggc gtggggcaaa gctcggaccg cggcagccgt
     3841 tctcaggagg aggtatctga gagcagctcg agcgcagacc ccctgcctaa tagctacctc
     3901 cctgattcat cgtctgtgtc tcatgggcca gtggcagggg tgacaggcgg tcccccagca
     3961 cttgtgcact ctagtgcact cccagacccc aacatgctgg tgtccgactg cacagcttcc
     4021 tcctcggacc tgggctcggc catcgacaag atcatcgagt ccaccatcgg gcccgacctc
     4081 atccagaact gcatcactgt gaccagtgct gaggatggcg gggccgagac cacacggtac
     4141 ctgatcctac agggcccaga tgatggagcc cccatgacat caccaatgtc cagttccacc
     4201 ttggcccaca gcctagcagc cattgaggcc ctggcagatg gccccacatc cacatccaca
     4261 tgcctggagg cacagggtgg gcccagctcc ccggtgcagc tgcccccagc ctccggtgcc
     4321 gaagagccgg acctgcagag cctggaggcc atgatggagg tggtggtggt gcagcagttc
     4381 aaatgcaaga tgtgccagta ccggagcagc accaaggcca cactgctgcg ccacatgcgg
     4441 gaacgccact tccgtccagt agcagcagcc gcagcagcag ctggtaaaaa aggacgtcta
     4501 cggaagtgga gcacctccac caagagccaa gaggaagagg gaccagagga ggaggacgat
     4561 gatgacattg tagacgctgg agccattgat gacctggagg aggatagcga ctataatcca
     4621 gctgaggatg agccccgagg ccggcagctt cggctccagc gccccacccc cagtacccca
     4681 aggccccgaa ggagacctgg ccggccccgg aagctgcccc gcctggagat ctcagacctc
     4741 ccagatggtg tggaaggaga gcctctagtg agttcccaga gtggacagag ccctccagag
     4801 ccacaggatc ccgaggctcc cagctcctca ggcccaggac acctggtggc catgggcaag
     4861 gtgagcagga cccctgtgga agctggtgtg agccagtcag atgcagagaa cgcagccccc
     4921 tcctgcccgg atgagcatga cactctgccc cggcgccgag gtcgaccttc caggcgcttc
     4981 ctaggcaaga aataccgcaa gtactattac aagtcgccca aaccactttt gaggcccttc
     5041 ctgtgccgca tctgtggttc tcgctttctg tcccacgagg acctgcgctt ccacgtcaac
     5101 tcccatgagg ctggcgatcc ccagctcttc aagtgcctgc agtgcagcta tcgttcccgc
     5161 cgctggtcct cgctcaagga gcacatgttc aaccacgtgg gcagcaagcc ctacaagtgt
     5221 gacgagtgca gctacaccag tgtctaccgg aaggacgtca ttcggcacgc cgctgtgcac
     5281 agccgggacc ggaagaagag gccagatccg actccaaagc tgagctcttt cccctgccct
     5341 gtgtgtggcc gtgtgtaccc catgcagaaa agactcacgc agcacatgaa gacgcacagc
     5401 actgagaagc cccacatgtg tgacaagtgt ggaaagtcct ttaagaagcg ctacaccttc
     5461 aaaatgcacc tgctcacgca catccaggct gttgccaacc gcaggttcaa gtgtgagttc
     5521 tgtgagtttg tttgtgaaga caagaaggca ctgctgaacc accagttgtc ccacgtcagt
     5581 gacaagccct tcaaatgcag cttttgtccc taccgcacct tccgagagga cttcttgctg
     5641 tcccatgtgg ctgtcaagca cacaggggcc aagcccttcg cctgtgagta ctgccacttc
     5701 agcacacggc acaagaagaa cctgcgcctg cacgtacggt gccgacacgc aagcagcttc
     5761 gaggaatggg ggaggcgcca ccctgaggag cccccctccc gccgtcgccc cttcttctct
     5821 ctgcagcaga ttgaggagct gaagcagcag cacagtgcgg cccctggacc acctcccagt
     5881 tccccaggac ctcctgagat acccccagag gcgacaactt tccagtcatc tgaggctccc
     5941 tcattgctct gttctgacac cctgggcggc gccaccatca tctaccagca aggagctgag
     6001 gagtcgacag cgatggccac gcagacagcc ttggatcttc tgctgaacat gagtgctcag
     6061 cgggaactgg ggggcacagc cctgcaggtg gctgtggtga agtcggaaga tgtggaagca
     6121 gggttagcat cccctggtgg gcagccctcc cctgaaggtg ccactccaca ggtggtcacc
     6181 ctccacgtgg cagagccagg gggcggtgca gcagccgaga gccagctagg ccctcctgac
     6241 ctaccgcaga tcaccctggc acctggtcca tttggtggga ctggctacag tgtcatcaca
     6301 gcacccccta tggaggaggg aacatcagct cctggcacac cttacagcga ggagcccgca
     6361 ggagaggcag cccaggctgt ggttgtgagt gacaccctaa aagaagctgg cacccactac
     6421 atcatggcta ctgatggtac ccagttgcac cacattgagc tcaccgcaga tggctccatc
     6481 tccttcccaa gtccagatgc tctggcctct ggtgccaaat ggcccctgct gcagtgtggg
     6541 ggactgccca gagacggccc tgagccccca tctccagcca agacccactg cgtaggggac
     6601 tcccagagct ctgcctcctc acctcctgca accagcaaag ccctgggcct ggcagtgccc
     6661 ccgtcaccgc catctgcagc cactgctgca tcaaagaagt tttcctgcaa gatctgtgcc
     6721 gaggccttcc ctggccgagc tgagatggag agtcacaagc gggcccacgc tgggcctggt
     6781 gccttcaagt gccccgactg ccccttcagt gcccgccagt ggcccgaggt ccgggcgcac
     6841 atggcacagc actcaagcct acggccccac cagtgtagcc agtgcagctt tgcctccaag
     6901 aacaagaagg acctgcgtcg gcacatgctg actcacacaa aggagaagcc ttttgcatgc
     6961 cacctctgcg ggcagcgttt caaccgtaac gggcacctca agttccacat ccagcggctg
     7021 cacagtcctg atgggaggaa gtcaggaacc cctacagccc gggcccctac ccagacccca
     7081 acccagacca tcatcctgaa cagtgatgac gaaacactgg ccaccctgca cactgcactc
     7141 cagtccagtc acggggtcct gggcccagag cggctacagc aggcactgag ccaggaacac
     7201 atcatcgttg cccaggaaca gacagtgacc aatcaggagg aagccgccta catccaagag
     7261 atcaccacgg cagatggcca gaccgtacag cacctggtga cctccgacaa ccaggtgctg
     7321 tatatcatct cccaggatgg tgtccagcac ctgctccccc aggaatatgt tgtggtccct
     7381 gaaggccatc acatccaggt acaggagggc cagatcacac acatccagta tgaacaagga
     7441 gccccgttcc ttcaggagtc ccagatccag tatgtgcctg tgtccccagg ccagcagctt
     7501 gtcacacagg ctcaacttga ggctgcagca cactcagctg tcacagcagt ggctgatgct
     7561 gccatggccc aagcccaggg cctgtttggt acagacgaga cagtgcccga acacattcaa
     7621 cagctgcagc accagggcat cgagtacgac gtcatcaccc tggccgatga ctaggaattc
     7681 cttaagtacg taccgcgggc tagcggatcc ctgcagacat gtgatatcac gcgtcctcga
     7741 ctgtgccttc taatgcatgg ggttggggtt gcgccttttc caaggcagcc ctgggtttgc
     7801 gcagggacgc ggctgctctg ggcgtggttc cgggaaacgc agcggcgccg accctgggtc
     7861 tcgcacattc ttcacgtccg ttcgcagcgt cacccggatc ttcgccgcta cccttgtggg
     7921 ccccccggcg acgcttcctg ctccgcccct aagtcgggaa ggttccttgc ggttcgcggc
     7981 gtgccggacg tgacaaacgg aagccgcacg tctcactagt accctcgcag acggacagcg
     8041 ccagggagca atggcagcgc gccgaccgcg atgggctgtg gccaatagcg gctgctcagc
     8101 agggcgcgcc gagagcagcg gccgggaagg ggcggtgcgg gaggcggggt gtggggcggt
     8161 agtgtgggcc ctgttcctgc ccgcgcggtg ttccgcattc tgcaagcctc cggagcgcac
     8221 gtcggcagtc ggctccctcg ttgaccgaat caccgacctc tctccccagc atcgatatga
     8281 ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca
     8341 ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc
     8401 acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg
     8461 gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg
     8521 tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc
     8581 ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg
     8641 cgtggttcct ggccaccgtc ggagtctcgc ccgaccacca gggcaagggt ctgggcagcg
     8701 ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga
     8761 cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg
     8821 tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgattaa
     8881 ttaaaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
     8941 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
     9001 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
     9061 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
     9121 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
     9181 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
     9241 gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct
     9301 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
     9361 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
     9421 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcccgctga
     9481 gcaataacta gcggcgcgtg ctttaagacc aatgacttac aaggcagctg tagatcttag
     9541 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga
     9601 tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     9661 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     9721 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     9781 agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt atttataact
     9841 tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta
     9901 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     9961 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc
    10021 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
    10081 tgactaattt tttttattta tgcagaggcc gaggccgcct gtgcactttt cggggaaatg
    10141 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
    10201 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
    10261 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
    10321 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
    10381 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
    10441 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
    10501 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
    10561 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
    10621 taaccatgag tgataacact gcggccaact tacttctgac aacgattgga ggaccgaagg
    10681 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
    10741 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
    10801 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
    10861 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
    10921 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
    10981 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
    11041 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
    11101 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
    11161 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
    11221 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
    11281 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
    11341 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
    11401 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
    11461 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
    11521 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
    11581 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
    11641 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
    11701 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
    11761 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
    11821 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaaca
    11881 tatgt
//
