LOCUS       sequence-376105-        6005 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pdfr-1(2-1k) fluorescent neural reporter driving nuclear mTagBFP2 
            expression (refer to NeuroPAL paper for expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6005)
  AUTHORS   Yemini E, Lin A, Nejatbakhsh A, Varol E, Sun R, Mena GE, Samuel ADT,
            Paninski L, Venkatachalam V, Hobert O
  TITLE     NeuroPAL: A Multicolor Atlas for Whole-Brain Neuronal Identification
            in C. elegans.
  JOURNAL   Cell. 2021 Jan 7;184(1):272-288.e11. doi: 
            10.1016/j.cell.2020.12.012. Epub 2020 Dec 29.
  PUBMED    33378642
REFERENCE   2  (bases 1 to 6005)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.012"; journalName: "Cell"; date: "2021-01-7-
            7"; volume: "184"; issue: "1"; pages: "272-288.e11"
COMMENT     Sequence Label: pEY23
FEATURES             Location/Qualifiers
     source          1..6005
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6..22
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1370..1390
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1412..1432
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(3596..3618)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3718..3737
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(3815..3833)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3901..4005
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4006..4074,4075..4866)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4224..4243)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5037..5625
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5526..5545
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5779..5796
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5913..5934
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5949..5954,5955..5972,5973..5979)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5987..6003
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(5992..6005,1..9)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcctgcaga attacttcgg
      181 acgggtctag acgtttttaa gaaaaatagg gttaaaaaca ggccaagttt tggccatttg
      241 aaaaaagaaa tcgctcgagg caaaatttta aaaattcaaa cattatctac caacaatcaa
      301 gttacgtagg ttacagttga aatttatgga aatatagtaa actagctcta agatacattt
      361 tccataggat acatttttag aataggcttt ccaaaaaatc aagtcgtcta actaaaagtt
      421 taagaatttt taacatctat aaaatattaa cagcctcata tgagtatttt ttataatatt
      481 tccacgattg ctttttgttg tataactaag tgtttttttt tcttatatct tgtctttttt
      541 ctctttcacc aaatctctat gcttctgctt agggcacgcc ttcccatttt cttttcttta
      601 ttttcatttg tctcctccca ccctccaaat gtcttgaaac tgaaaacaaa ccaatgaata
      661 tatttataag tatctcttga gcttcaaaac ttttccattt tctgttctgt tttctttttc
      721 ttctacagaa tttttttgct aaattacgat ctttaggcac agcttctcaa aattaggaat
      781 ttcccttttt ttcaatattt gttcaccgtt tttgagtaaa atgagacgtt ttcgccttct
      841 tttctatgaa tatctaaata tgacctctta taacgtctcg ttttcttcaa aaaatgtgtt
      901 atttttaacg gtttcttcct cccagtggga ggttgttgcg tgggggcttc gtccattttt
      961 ttcaaacctt ttccatcaaa aagttcccac aattaacata aatatctcct acgcaaatgc
     1021 gtcatttttt gtttgggaaa cgcaaattat aatcagcggg ttattaaatt ttcggaacga
     1081 ctgagctctt aaatatccag actgctaaca ttttgacttg atttattagt tttcctattt
     1141 tttgatggag ttatagttgt aatttagatc taaccaggct tcaaaaaaaa aatagataaa
     1201 atatttgatt aacaacattc aggaacatgt cacgcaataa agctggttag ggaagcccgg
     1261 gattggccaa aggacccaaa ggtatgtttc gaatgatact aacataacat agaacatttt
     1321 caggaggacc cttgcttgga gggtaccgag ctcagaaaaa atgaccgctc caaagaagaa
     1381 gcgcaaggtt ccagtcgaga agatgactgc cccaaaaaag aaacgtaagg tcccgcggat
     1441 gtcagagctt attaaggaga atatgcatat gaaactgtac atggaaggaa ccgtggacaa
     1501 ccatcacttt aagtgcactt cggaggggga aggcaaaccg tacgagggca cccagaccat
     1561 gcgaatcaag gtggtcgagg gaggcccact gcccttcgcc ttcgacattc tggtaagttt
     1621 aaacatatat atactaacta accctgatta tttaaatttt caggctacta gcttcctgta
     1681 cggcagcaag acttttatta atcacaccca gggcatcccg gacttcttca aacagtcgtt
     1741 ccctgagggc ttcacatggg agcgtgtgac cacatacgaa gacggtggcg ttctcaccgc
     1801 tacccaggac accagcctcc aggacggttg cctcatatat aatgtggtaa gtttaaacag
     1861 ttcggtacta actaaccata catatttaaa ttttcagaag atccggggag taaacttcac
     1921 atccaatggt ccggttatgc agaagaaaac actcggctgg gaggccttca ccgagacgct
     1981 gtatccggct gacggaggcc tggaaggtcg aaacgacatg gccctgaagc tcgtcggcgg
     2041 tagccatctc atcgcaaacg ctaagaccac atatagatcc aagaaaccgg taagtttaaa
     2101 catgatttta ctaactaact aatctgattt aaattttcag gctaagaacc ttaaaatgcc
     2161 gggagtctac tatgttgact acagactgga acggatcaag gaggccaaca acgagaccta
     2221 cgtcgagcag cacgaggttg cagttgccag atactgcgac ctcccgagca aactgggtca
     2281 caagcttaat cctaggatac cggtgggtga agacccacca aagccatctg ccaagggagc
     2341 caagaaggcc gccaagaccg ttacgaagcc aaaggacgga aagaagagac gtcatgcccg
     2401 taaggaatca tactccgtct acatctaccg tgtcctcaag caagttcatc cagacactgg
     2461 agtttcctcc aaagccatgt ctatcatgaa ctcttttgtc aacgatgtct tcgagcgtat
     2521 tgctgctgaa gcatcccgtc ttgctcacta caacaagcgt tccacaatct catcccgcga
     2581 aattcagacc gctgtccgtc tgatccttcc aggagagctt gccaagcacg ccgtgtctga
     2641 gggaaccaag gccgttacca agtacacttc cagcaagtag gaattccaac tgagcgccgg
     2701 tcgctaccat taccaacttg tctggtgtca aaaataatag gggccgctgt catcagagta
     2761 agtttaaact gagttctact aactaacgag taatatttaa attttcagca tctcgcgccc
     2821 gtgcctctga cttctaagtc caattactct tcaacatccc tacatgctct ttctccctgt
     2881 gctcccaccc cctatttttg ttattatcaa aaaacttctc ttaatttctt tgttttttag
     2941 cttcttttaa gtcacctcta acaatgaaat tgtgtagatt caaaaataga attaattcgt
     3001 aataaaaagt cgaaaaaaat tgtgctccct ccccccatta ataataattc tatcccaaaa
     3061 tctacacaat gttctgtgta cacttcttat gttttttact tctgataaat ttttttgaaa
     3121 catcatagaa aaaaccgcac acaaaatacc ttatcatatg ttacgtttca gtttatgacc
     3181 gcaattttta tttcttcgca cgtctgggcc tctcatgacg tcaaatcatg ctcatcgtga
     3241 aaaagttttg gagtattttt ggaatttttc aatcaagtga aagtttatga aattaatttt
     3301 cctgcttttg ctttttgggg tttcccctat tgtttgtcaa gatttcgagg acggcgtttt
     3361 tcttgctaaa atcacaagta ttgatgagca cgatgcaaga aagatcggaa gaaggtttgg
     3421 gtttgaggct cagtggaagg tgagtagaag ttgataattt gaaagtggag tagtgtctat
     3481 ggggtttttg ccttaaatga cagaatacat tcccaatata ccaaacataa ctgtttccta
     3541 ctagtcggcc gtacgggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct
     3601 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac
     3661 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg
     3721 catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg
     3781 taaggagaaa ataccgcatc aggcggcctt aagggcctcg tgatacgcct atttttatag
     3841 gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg
     3901 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     3961 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     4021 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     4081 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     4141 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     4201 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     4261 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     4321 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     4381 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     4441 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     4501 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     4561 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     4621 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     4681 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     4741 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     4801 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     4861 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     4921 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     4981 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     5041 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     5101 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     5161 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     5221 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     5281 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     5341 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     5401 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     5461 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     5521 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     5581 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     5641 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     5701 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     5761 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     5821 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
     5881 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
     5941 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
     6001 caatt
//
