LOCUS       sequence-376106-        6876 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  gcy-36 fluorescent neural reporter driving nuclear CYOFP expression 
            (refer to NeuroPAL paper for expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6876)
  AUTHORS   Yemini E, Lin A, Nejatbakhsh A, Varol E, Sun R, Mena GE, Samuel ADT,
            Paninski L, Venkatachalam V, Hobert O
  TITLE     NeuroPAL: A Multicolor Atlas for Whole-Brain Neuronal Identification
            in C. elegans.
  JOURNAL   Cell. 2021 Jan 7;184(1):272-288.e11. doi: 
            10.1016/j.cell.2020.12.012. Epub 2020 Dec 29.
  PUBMED    33378642
REFERENCE   2  (bases 1 to 6876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.012"; journalName: "Cell"; date: "2021-01-7-
            7"; volume: "184"; issue: "1"; pages: "272-288.e11"
COMMENT     Sequence Label: pEY29
FEATURES             Location/Qualifiers
     source          1..6876
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6..22
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2235..2255
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2277..2297
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(4467..4489)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4589..4608
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4686..4704)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4772..4876
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4877..4945,4946..5737)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5095..5114)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5908..6496
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6397..6416
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6650..6667
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6784..6805
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6820..6825,6826..6843,6844..6850)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6858..6874
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(6863..6876,1..9)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcctgcagt aaatgggtgt
      181 cgggtgcgta attgtaatgt ttggtaggtc ggcgagcgtt tttgctgttg gaaacgcaac
      241 atggttttcc actttctcgt tttttgaggt tttgtgtccc atcctaaaaa attcaagttg
      301 aaatttgtta ttggcatgta ttctcggcat tttgcattag gttcttgctt cagtttagcc
      361 cgaaaatgtt taacattgat taaaagttgg caaaactagg gccaaccttg gcgaaagttg
      421 tgcaagatct ttgggaaaaa aattaggctg agcacaagtt taagccaact ttaaaccagc
      481 tcaagtttga aacgacacca gaaatgtttg cacaaaagtt tggtaaaagt tgggtcaaac
      541 tcggcgctac tcaaattaag ttttgccaca atctcgtcca agttgcgcca aatgtaaccc
      601 aaattttggc aacgtttgat aaaaattaac atttccggca gagcaaaact tggccaagaa
      661 gctagtgcca aacgccgagt tataaatttt catacaaaag aaaacgagtg gtgaaaattt
      721 tgaaaattta gaaagtagaa tattgaatat acatagaata tgtgaaacgc gaagaataca
      781 ataggacgaa tcaacgcact ccgttttaat cgcatagtct tactaaaaat tgagagattg
      841 gatgaaaagc ggagagaaaa gtggtttgca acaaacacgg aaaatcagtt atacagtatt
      901 tttctccgca tctttttttt cccgagttac tggcacactt tttatttcca taaacctgca
      961 ctttatgcaa attacggtga atcattccag ccgtctggtc tatcattgac ttttgtttta
     1021 acaagtttta gatatgatgt tggtagatgg ggtttggatt catcgtgaat taatctcatt
     1081 gaaaatttga agtagttcgg attctgaaaa ggccatctga aaatgatcct aattccattt
     1141 ggaaagttga aaactgtaaa aatactaatt tcaggctttt cgtcgttgga actcagaata
     1201 tcacagtttt acgcactttc agttattttt aatgaacaca atgcatattt attgcttatg
     1261 ctttttattt tccgataaaa cgacacggaa tatgtaaaaa aaacagtcaa ataataaaaa
     1321 taaaaagaat taattaaata taatagtgca agcgcgctca atcgaaaaat ttttttttta
     1381 attttaaaat tttttattta aaaatttaaa aatttttctg aattttttaa tcatgatatt
     1441 cggccatagt aatggaatgg gatgtcccca taggaatgtt ttaaagcaat ttccccactg
     1501 agcagaattc atggtgaaac atttttttgc caatgggaag gttttattgt aaggtaccgt
     1561 attttcagtc atatctgaag tactaaaatc tggttgtttt aacattaaaa ctgcattttc
     1621 tgtgtaaata taaaagttat ttgaattaaa caattatacc acaaaagaaa tacgaaaaaa
     1681 aaattctaaa tccaaatttt cagtagcaac ttttcaattt tgactaactt aaggcagact
     1741 accgtttttc ttctattagt cttgcatgca tatcattttt agttgcattc catagatacc
     1801 ttttgagttt tccatatatt tacaatcttc ctcactctcc catctgtcac catctcagca
     1861 accggtgagg tcaggcggat aataaaattt ttgatgacat cgacacacgg aatgacttcc
     1921 taaaacagac gactacagca ttgcgtgtct attaattatt caatgcacct gagcaatgag
     1981 aagttacaat ttccaattcc accttttcgt aatcataact tttcttccat ttcgtaactc
     2041 attttggtaa gaatttccgt ctttcggagc gagaatattt cttttctgac gtggtaaatt
     2101 caaacaaggg ctacccaaca cccgggattg gccaaaggac ccaaaggtat gtttcgaatg
     2161 atactaacat aacatagaac attttcagga ggacccttgc ttggagggta ccgagctcag
     2221 aaaaaatgac cgctccaaag aagaagcgca aggttccagt cgagaagatg actgccccaa
     2281 aaaagaaacg taaggtcccg cgggtaagtt taaacagttc ggtactaact aaccatacat
     2341 atttaaattt tcagcgcgct gtgtcaaaag gagaagagct tattaaggag aatatgcgtt
     2401 ccaagctcta cctcgaggga tccgtcaacg gacaccaatt caagtgcacc cacgagggag
     2461 agggaaagcc atacgaggga aagcaaacca accgtatcaa ggtcgtcgag ggaggaccac
     2521 tcccattcgc cttcgacatc ctcgccaccc acttcatgta cggatccaag gtaagtttaa
     2581 acatatatat actaactaac cctgattatt taaattttca ggtcttcatc aagtacccag
     2641 ccgacctccc agactacttc aagcaatcct tcccagaggg attcacctgg gagcgtgtca
     2701 tggtcttcga ggacggagga gtcctcaccg ccacccaaga cacctccctc caagacggag
     2761 agctcatcta caacgtcaag gtccgtggag tcaacttccc agccaacgga ccagtcatgc
     2821 aaaagaagac cctcggatgg gagccatcca ccgagaccat gtacccagcc gacggaggac
     2881 tcgagggacg ttgcgacaag gtaagtttaa acatgatttt actaactaac taatctgatt
     2941 taaattttca ggccctcaag ctcgtcggag gaggacacct ccacgtcaac ttcaagacca
     3001 cctacaagtc caagaagcca gtcaagatgc caggagtcca ctacgtcgac cgtcgtctcg
     3061 agcgtatcaa ggaggccgac aacgagacct acgtcgagca atacgagcac gccgtcgccc
     3121 gttactccaa cctcggagga ggaatggacg agctctacaa gcctaggata ccggtgggtg
     3181 aagacccacc aaagccatct gccaagggag ccaagaaggc cgccaagacc gttacgaagc
     3241 caaaggacgg aaagaagaga cgtcatgccc gtaaggaatc atactccgtc tacatctacc
     3301 gtgtcctcaa gcaagttcat ccagacactg gagtttcctc caaagccatg tctatcatga
     3361 actcttttgt caacgatgtc ttcgagcgta ttgctgctga agcatcccgt cttgctcact
     3421 acaacaagcg ttccacaatc tcatcccgcg aaattcagac cgctgtccgt ctgatccttc
     3481 caggagagct tgccaagcac gccgtgtctg agggaaccaa ggccgttacc aagtacactt
     3541 ccagcaagta ggaattccaa ctgagcgccg gtcgctacca ttaccaactt gtctggtgtc
     3601 aaaaataata ggggccgctg tcatcagagt aagtttaaac tgagttctac taactaacga
     3661 gtaatattta aattttcagc atctcgcgcc cgtgcctctg acttctaagt ccaattactc
     3721 ttcaacatcc ctacatgctc tttctccctg tgctcccacc ccctattttt gttattatca
     3781 aaaaacttct cttaatttct ttgtttttta gcttctttta agtcacctct aacaatgaaa
     3841 ttgtgtagat tcaaaaatag aattaattcg taataaaaag tcgaaaaaaa ttgtgctccc
     3901 tccccccatt aataataatt ctatcccaaa atctacacaa tgttctgtgt acacttctta
     3961 tgttttttac ttctgataaa tttttttgaa acatcataga aaaaaccgca cacaaaatac
     4021 cttatcatat gttacgtttc agtttatgac cgcaattttt atttcttcgc acgtctgggc
     4081 ctctcatgac gtcaaatcat gctcatcgtg aaaaagtttt ggagtatttt tggaattttt
     4141 caatcaagtg aaagtttatg aaattaattt tcctgctttt gctttttggg gtttccccta
     4201 ttgtttgtca agatttcgag gacggcgttt ttcttgctaa aatcacaagt attgatgagc
     4261 acgatgcaag aaagatcgga agaaggtttg ggtttgaggc tcagtggaag gtgagtagaa
     4321 gttgataatt tgaaagtgga gtagtgtcta tggggttttt gccttaaatg acagaataca
     4381 ttcccaatat accaaacata actgtttcct actagtcggc cgtacgggcc ctttcgtctc
     4441 gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca
     4501 gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt
     4561 ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac
     4621 catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcggcct
     4681 taagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
     4741 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     4801 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     4861 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     4921 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     4981 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     5041 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     5101 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     5161 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     5221 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     5281 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     5341 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     5401 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     5461 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     5521 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     5581 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     5641 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     5701 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     5761 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     5821 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     5881 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     5941 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     6001 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     6061 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     6121 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     6181 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     6241 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     6301 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     6361 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     6421 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     6481 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     6541 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     6601 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     6661 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
     6721 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
     6781 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc
     6841 tcgtatgttg tgtggaattg tgagcggata acaatt
//
