LOCUS       sequence-376108-        5649 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  cho-1(3.3-2.6k) fluorescent neural reporter driving nuclear CYOFP 
            expression (refer to NeuroPAL paper for expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5649)
  AUTHORS   Yemini E, Lin A, Nejatbakhsh A, Varol E, Sun R, Mena GE, Samuel ADT,
            Paninski L, Venkatachalam V, Hobert O
  TITLE     NeuroPAL: A Multicolor Atlas for Whole-Brain Neuronal Identification
            in C. elegans.
  JOURNAL   Cell. 2021 Jan 7;184(1):272-288.e11. doi: 
            10.1016/j.cell.2020.12.012. Epub 2020 Dec 29.
  PUBMED    33378642
REFERENCE   2  (bases 1 to 5649)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.012"; journalName: "Cell"; date: "2021-01-7-
            7"; volume: "184"; issue: "1"; pages: "272-288.e11"
COMMENT     Sequence Label: pEY26
FEATURES             Location/Qualifiers
     source          1..5649
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6..22
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1008..1028
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1050..1070
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(3240..3262)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3362..3381
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(3459..3477)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3545..3649
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3650..3718,3719..4510)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3868..3887)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4681..5269
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5170..5189
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5423..5440
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5557..5578
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5593..5598,5599..5616,5617..5623)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5631..5647
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(5636..5649,1..9)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcctgcagt ttcatctctc
      181 gcggccacgt aggatactag aaagaagagt gccctctaga aaactcggcc acgggtgcgc
      241 ggaatttcaa atttcctctg ggtttttgaa gattccgagc aactttcgtt ttagtctagg
      301 tccgagaaaa ttcgcggccg ccaatttttt acggccatgt aggaaaaaat tcattttagg
      361 agcattgaat gatattgtag gtcctaccga atttctaaag cgattaccgt acccgtttaa
      421 aggcgcatgc cacttttcac cacccttcac gacctataaa tttattttcg atagttgcaa
      481 aaaaagcgaa gaaaacaaga aacgcttgac aaaacatttc cgcagttgca aatcctttct
      541 taaaacttgt ttgattggct caatccgttg ctcttttgct catcctcctc tctttcccat
      601 acacacacat cgaaatatga atcttctctt aaaaagaagg ttgtccaatt agtttcccct
      661 attcattttt ccatgttttc ttataacaac tttttctttt gaatactcct ccagaaatga
      721 taaatctttg tgagatgcca attaaaattt atttttgaat tttatttgtt gaaggatttt
      781 atttgttgaa tgacctaaat agaggctccg cccatatctt ggctttctgc aatttgcata
      841 aaaggttttc cagttttcgt aggtattttt tgaaggtctt ccacgtaggc gttcccggga
      901 ttggccaaag gacccaaagg tatgtttcga atgatactaa cataacatag aacattttca
      961 ggaggaccct tgcttggagg gtaccgagct cagaaaaaat gaccgctcca aagaagaagc
     1021 gcaaggttcc agtcgagaag atgactgccc caaaaaagaa acgtaaggtc ccgcgggtaa
     1081 gtttaaacag ttcggtacta actaaccata catatttaaa ttttcagcgc gctgtgtcaa
     1141 aaggagaaga gcttattaag gagaatatgc gttccaagct ctacctcgag ggatccgtca
     1201 acggacacca attcaagtgc acccacgagg gagagggaaa gccatacgag ggaaagcaaa
     1261 ccaaccgtat caaggtcgtc gagggaggac cactcccatt cgccttcgac atcctcgcca
     1321 cccacttcat gtacggatcc aaggtaagtt taaacatata tatactaact aaccctgatt
     1381 atttaaattt tcaggtcttc atcaagtacc cagccgacct cccagactac ttcaagcaat
     1441 ccttcccaga gggattcacc tgggagcgtg tcatggtctt cgaggacgga ggagtcctca
     1501 ccgccaccca agacacctcc ctccaagacg gagagctcat ctacaacgtc aaggtccgtg
     1561 gagtcaactt cccagccaac ggaccagtca tgcaaaagaa gaccctcgga tgggagccat
     1621 ccaccgagac catgtaccca gccgacggag gactcgaggg acgttgcgac aaggtaagtt
     1681 taaacatgat tttactaact aactaatctg atttaaattt tcaggccctc aagctcgtcg
     1741 gaggaggaca cctccacgtc aacttcaaga ccacctacaa gtccaagaag ccagtcaaga
     1801 tgccaggagt ccactacgtc gaccgtcgtc tcgagcgtat caaggaggcc gacaacgaga
     1861 cctacgtcga gcaatacgag cacgccgtcg cccgttactc caacctcgga ggaggaatgg
     1921 acgagctcta caagcctagg ataccggtgg gtgaagaccc accaaagcca tctgccaagg
     1981 gagccaagaa ggccgccaag accgttacga agccaaagga cggaaagaag agacgtcatg
     2041 cccgtaagga atcatactcc gtctacatct accgtgtcct caagcaagtt catccagaca
     2101 ctggagtttc ctccaaagcc atgtctatca tgaactcttt tgtcaacgat gtcttcgagc
     2161 gtattgctgc tgaagcatcc cgtcttgctc actacaacaa gcgttccaca atctcatccc
     2221 gcgaaattca gaccgctgtc cgtctgatcc ttccaggaga gcttgccaag cacgccgtgt
     2281 ctgagggaac caaggccgtt accaagtaca cttccagcaa gtaggaattc caactgagcg
     2341 ccggtcgcta ccattaccaa cttgtctggt gtcaaaaata ataggggccg ctgtcatcag
     2401 agtaagttta aactgagttc tactaactaa cgagtaatat ttaaattttc agcatctcgc
     2461 gcccgtgcct ctgacttcta agtccaatta ctcttcaaca tccctacatg ctctttctcc
     2521 ctgtgctccc accccctatt tttgttatta tcaaaaaact tctcttaatt tctttgtttt
     2581 ttagcttctt ttaagtcacc tctaacaatg aaattgtgta gattcaaaaa tagaattaat
     2641 tcgtaataaa aagtcgaaaa aaattgtgct ccctcccccc attaataata attctatccc
     2701 aaaatctaca caatgttctg tgtacacttc ttatgttttt tacttctgat aaattttttt
     2761 gaaacatcat agaaaaaacc gcacacaaaa taccttatca tatgttacgt ttcagtttat
     2821 gaccgcaatt tttatttctt cgcacgtctg ggcctctcat gacgtcaaat catgctcatc
     2881 gtgaaaaagt tttggagtat ttttggaatt tttcaatcaa gtgaaagttt atgaaattaa
     2941 ttttcctgct tttgcttttt ggggtttccc ctattgtttg tcaagatttc gaggacggcg
     3001 tttttcttgc taaaatcaca agtattgatg agcacgatgc aagaaagatc ggaagaaggt
     3061 ttgggtttga ggctcagtgg aaggtgagta gaagttgata atttgaaagt ggagtagtgt
     3121 ctatggggtt tttgccttaa atgacagaat acattcccaa tataccaaac ataactgttt
     3181 cctactagtc ggccgtacgg gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac
     3241 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
     3301 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat
     3361 gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga
     3421 tgcgtaagga gaaaataccg catcaggcgg ccttaagggc ctcgtgatac gcctattttt
     3481 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa
     3541 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     3601 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     3661 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     3721 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     3781 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     3841 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     3901 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     3961 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     4021 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     4081 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     4141 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     4201 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     4261 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     4321 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     4381 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     4441 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     4501 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     4561 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     4621 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     4681 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     4741 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     4801 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     4861 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     4921 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     4981 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     5041 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     5101 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     5161 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     5221 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     5281 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     5341 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     5401 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
     5461 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
     5521 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
     5581 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
     5641 ataacaatt
//
