LOCUS       sequence-376110-        5770 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  gcy-27 fluorescent neural reporter driving nuclear mTagBFP2 
            expression (refer to NeuroPAL paper for expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5770)
  AUTHORS   Yemini E, Lin A, Nejatbakhsh A, Varol E, Sun R, Mena GE, Samuel ADT,
            Paninski L, Venkatachalam V, Hobert O
  TITLE     NeuroPAL: A Multicolor Atlas for Whole-Brain Neuronal Identification
            in C. elegans.
  JOURNAL   Cell. 2021 Jan 7;184(1):272-288.e11. doi: 
            10.1016/j.cell.2020.12.012. Epub 2020 Dec 29.
  PUBMED    33378642
REFERENCE   2  (bases 1 to 5770)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.012"; journalName: "Cell"; date: "2021-01-7-
            7"; volume: "184"; issue: "1"; pages: "272-288.e11"
COMMENT     Sequence Label: pEY87
FEATURES             Location/Qualifiers
     source          1..5770
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6..22
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1135..1155
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1177..1197
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(3361..3383)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3483..3502
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(3580..3598)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3666..3770
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3771..3839,3840..4631)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3989..4008)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4802..5390
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5291..5310
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5544..5561
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5678..5699
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5714..5719,5720..5737,5738..5744)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5752..5768
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(5757..5770,1..9)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcctgcaga ccgaatagac
      181 aaccaaactt cattgatgtt gaatgaaaag ttttagtcag aaaactgtcg gttttaggtg
      241 cagctttgat tgaggcgaac aaaacaaaat tcaaaggttt tattgatatg ataattttat
      301 ttgtccgcga tcagctaaag atgtaaatag ttttttcttg acaattgacg tagaagaaga
      361 gaaaaatatg aagaaactga aagatgcgaa caaatacttt aataattctg ggcaaaactt
      421 ggaaatttta tgaaacttta aaaaaataat atttgtgcat tgataccttt cgctgaaaca
      481 ctagtgtttg aaaaaatcag ataaaagttt ggcaaattat attctcatgc tcgtctgttt
      541 ttttcaaaat gtaattttga aagtaaatct tggggaacaa aacattatcc ttttcagctg
      601 gtacagtttt ttgtaggaat tacagttttc ttctcctgaa aactaaaacg atattaaaaa
      661 ttttataaac acttttttcg agtacaaata ggttttagtt acatgtttga taaactttca
      721 taaattaact cgcgaaatca taatgttgtt tgtttgaaat ctaggccaat accaaaacca
      781 aaattggcat gagacaagtt ttcaattaga acactttttt ctatactatc atcaaatgtt
      841 cctcaagttt taatgcccgt taggttgcac ttttattttg tctttgcctc ctcacaagtt
      901 tttcttgtta atgagtagtt gtgaacatac tcatcaagta tcggttgcca tggaaaaata
      961 actcacacat ttcagagtct ttctcgagat acaactttca ttttatttat tttctaccaa
     1021 cccgggattg gccaaaggac ccaaaggtat gtttcgaatg atactaacat aacatagaac
     1081 attttcagga ggacccttgc ttggagggta ccgagctcag aaaaaatgac cgctccaaag
     1141 aagaagcgca aggttccagt cgagaagatg actgccccaa aaaagaaacg taaggtcccg
     1201 cggatgtcag agcttattaa ggagaatatg catatgaaac tgtacatgga aggaaccgtg
     1261 gacaaccatc actttaagtg cacttcggag ggggaaggca aaccgtacga gggcacccag
     1321 accatgcgaa tcaaggtggt cgagggaggc ccactgccct tcgccttcga cattctggta
     1381 agtttaaaca tatatatact aactaaccct gattatttaa attttcaggc tactagcttc
     1441 ctgtacggca gcaagacttt tattaatcac acccagggca tcccggactt cttcaaacag
     1501 tcgttccctg agggcttcac atgggagcgt gtgaccacat acgaagacgg tggcgttctc
     1561 accgctaccc aggacaccag cctccaggac ggttgcctca tatataatgt ggtaagttta
     1621 aacagttcgg tactaactaa ccatacatat ttaaattttc agaagatccg gggagtaaac
     1681 ttcacatcca atggtccggt tatgcagaag aaaacactcg gctgggaggc cttcaccgag
     1741 acgctgtatc cggctgacgg aggcctggaa ggtcgaaacg acatggccct gaagctcgtc
     1801 ggcggtagcc atctcatcgc aaacgctaag accacatata gatccaagaa accggtaagt
     1861 ttaaacatga ttttactaac taactaatct gatttaaatt ttcaggctaa gaaccttaaa
     1921 atgccgggag tctactatgt tgactacaga ctggaacgga tcaaggaggc caacaacgag
     1981 acctacgtcg agcagcacga ggttgcagtt gccagatact gcgacctccc gagcaaactg
     2041 ggtcacaagc ttaatcctag gataccggtg ggtgaagacc caccaaagcc atctgccaag
     2101 ggagccaaga aggccgccaa gaccgttacg aagccaaagg acggaaagaa gagacgtcat
     2161 gcccgtaagg aatcatactc cgtctacatc taccgtgtcc tcaagcaagt tcatccagac
     2221 actggagttt cctccaaagc catgtctatc atgaactctt ttgtcaacga tgtcttcgag
     2281 cgtattgctg ctgaagcatc ccgtcttgct cactacaaca agcgttccac aatctcatcc
     2341 cgcgaaattc agaccgctgt ccgtctgatc cttccaggag agcttgccaa gcacgccgtg
     2401 tctgagggaa ccaaggccgt taccaagtac acttccagca agtaggaatt ccaactgagc
     2461 gccggtcgct accattacca acttgtctgg tgtcaaaaat aataggggcc gctgtcatca
     2521 gagtaagttt aaactgagtt ctactaacta acgagtaata tttaaatttt cagcatctcg
     2581 cgcccgtgcc tctgacttct aagtccaatt actcttcaac atccctacat gctctttctc
     2641 cctgtgctcc caccccctat ttttgttatt atcaaaaaac ttctcttaat ttctttgttt
     2701 tttagcttct tttaagtcac ctctaacaat gaaattgtgt agattcaaaa atagaattaa
     2761 ttcgtaataa aaagtcgaaa aaaattgtgc tccctccccc cattaataat aattctatcc
     2821 caaaatctac acaatgttct gtgtacactt cttatgtttt ttacttctga taaatttttt
     2881 tgaaacatca tagaaaaaac cgcacacaaa ataccttatc atatgttacg tttcagttta
     2941 tgaccgcaat ttttatttct tcgcacgtct gggcctctca tgacgtcaaa tcatgctcat
     3001 cgtgaaaaag ttttggagta tttttggaat ttttcaatca agtgaaagtt tatgaaatta
     3061 attttcctgc ttttgctttt tggggtttcc cctattgttt gtcaagattt cgaggacggc
     3121 gtttttcttg ctaaaatcac aagtattgat gagcacgatg caagaaagat cggaagaagg
     3181 tttgggtttg aggctcagtg gaaggtgagt agaagttgat aatttgaaag tggagtagtg
     3241 tctatggggt ttttgcctta aatgacagaa tacattccca atataccaaa cataactgtt
     3301 tcctactagt cggccgtacg ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa
     3361 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag
     3421 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta
     3481 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag
     3541 atgcgtaagg agaaaatacc gcatcaggcg gccttaaggg cctcgtgata cgcctatttt
     3601 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
     3661 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     3721 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     3781 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     3841 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     3901 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     3961 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     4021 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     4081 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     4141 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     4201 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     4261 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     4321 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     4381 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     4441 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     4501 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     4561 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     4621 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     4681 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     4741 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     4801 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     4861 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     4921 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     4981 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     5041 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     5101 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     5161 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     5221 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     5281 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     5341 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     5401 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     5461 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     5521 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
     5581 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
     5641 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
     5701 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
     5761 gataacaatt
//
