LOCUS       sequence-376111-        7246 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  glr-8 fluorescent neural reporter driving nuclear CYOFP expression 
            (refer to NeuroPAL paper for expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7246)
  AUTHORS   Yemini E, Lin A, Nejatbakhsh A, Varol E, Sun R, Mena GE, Samuel ADT,
            Paninski L, Venkatachalam V, Hobert O
  TITLE     NeuroPAL: A Multicolor Atlas for Whole-Brain Neuronal Identification
            in C. elegans.
  JOURNAL   Cell. 2021 Jan 7;184(1):272-288.e11. doi: 
            10.1016/j.cell.2020.12.012. Epub 2020 Dec 29.
  PUBMED    33378642
REFERENCE   2  (bases 1 to 7246)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.012"; journalName: "Cell"; date: "2021-01-7-
            7"; volume: "184"; issue: "1"; pages: "272-288.e11"
COMMENT     Sequence Label: pEY92
FEATURES             Location/Qualifiers
     source          1..7246
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6..22
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2605..2625
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2647..2667
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(4837..4859)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4959..4978
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(5056..5074)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5142..5246
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5247..5315,5316..6107)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5465..5484)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6278..6866
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6767..6786
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7020..7037
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7154..7175
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7190..7195,7196..7213,7214..7220)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7228..7244
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(7233..7246,1..9)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcctgcagc aaaccgtcgt
      181 caactgccgt cgaaggtgga gtctcaaaag tcaccggcat ctgaaaatat gatattagat
      241 tgaaaaaaat attgaactaa aagtgcatag gtaaagtaaa atcgaatcga aattgagcga
      301 gcaacgagaa gtgcattgca tattttatca attccaattc cttttcctgc gcgcgcgcca
      361 aatgtagagc tttttacgac ttttgcttta caaatagtca ttgcacgtca tgcaacgtaa
      421 acataaaaat cttttaaata ttcagaccaa ccccaatgtt tgatttgaat cattggtaca
      481 acaatgaaat tgaaattcga tgaaacattc gatgcaaaaa tagtcaaaaa tgatacctca
      541 tgatgaatga tgaaaatacg agacaggaaa caggaaaata tcagtgagta tttgtatttg
      601 tggcaatgca cggatatgag aaaatgacat ctcgttcagt atttattctg gtctctcgcc
      661 gacgacaaga tgttgttttt ggtttgaggg gcgaaaaata tcacaaaatg caaattgctg
      721 gagacggaag acaaagcagt agtcgatata aacatagtga cactcgcaaa taaggcctac
      781 tgcgcgaaca caagatgcgc aaaggaaacg gaatatgcgc aagcaacgct gttggcgtac
      841 atgacatttt tgaattgaaa ttagttataa aacattaaaa ccattatatt ttcgacatta
      901 ctggaaaaaa aaagcaaaaa agcagtgtta aagtaaaatt caacaaataa attaggatat
      961 gagagaaatc gataatgtgt ggcctgcaat tgagggggag aaccggtcga ggagggagaa
     1021 gagggagagg tccgtgtttg agcgggcata gacgcagtat cgaatgtcaa tagaaatgca
     1081 ggtaactaat taggttttta tagttacttg gtgacacaaa aacaacaaca acaagttact
     1141 acaagaatct gataaaaggc aatgaaaaat tgaaataaat aaaaaaattt ctccaaaact
     1201 caattccaga ttttgtctta agacatttag tttattcttg tcgattttat attaaatttt
     1261 gaatttaaat actgatttca tcacttaaag tttggttata atttctatat taataatttt
     1321 tcaaactcct agattttttt atggtaacaa attctaaact acttaaattt ttcaaaaagc
     1381 aatcaaatcg caatcaagtt caaaaaattt attaaaatgc acacatacga acacacgtgc
     1441 atttggtttc acaatacgaa acttttcaat cgaaagtcac ttgaagtccg cttgagaggg
     1501 aacagacaaa cggaggaggt ttagagacaa agtggtgaga cactattaag ttcgcagaga
     1561 gtggtcgtgg cggaaaactt taattttaag acttttccag tatttttgag acatttgaat
     1621 ttttaaattg caaagatata attacggaaa ataatgtaaa aatattttac tataacactt
     1681 aaacacctcc acttaactca agagcgccta ctatgttttc aagtgatcca atttctggaa
     1741 atattgaaac atgagctctc aacaacgggc ggtattcaaa aattcatcag gaggtcggac
     1801 acacacacat tctaagctca ccatgcatat tctatgtgtc attattcttg ttcatgtttc
     1861 acagatagac tctcataagg ccatgttctc gccagagttc gaaaatacta cagctgatcc
     1921 aaattctatt aggacattaa ataatattct agagtctata aatgaaagta agttttgaca
     1981 aaacatattt acactttgca gatgtttaat aatgggactt tttttgaagt aaacaataaa
     2041 ttaaatttta gcgatttgct aaacctttga cggaaaactt tcaaaaaaat attggctcac
     2101 gtttacgttt agtttttgtt gcaaagtttg agaaaaccct taaatacggc taaaatggaa
     2161 atttgctgta acattttaaa gctgtttttt ttagcttaat gcaatttttt caaaaagcat
     2221 tcctaacatt tatcacaatt ttataaaatg aaaccctgga ctacggaatt atgtatttct
     2281 ttgcggtttg aaaaaataat ttcaaaaatt tcaacgtaga ttcataattt gttcagaagt
     2341 tgctcatccg cctcaaactt ccctaacgag cgactatgga gatgcatata ctgtaagtat
     2401 gaatatattc atcttaaaat aatagttcac tgaaaattag tgatcaaatt tttccagctc
     2461 agcaatgaaa aacacttttc gctttccgaa cccgggattg gccaaaggac ccaaaggtat
     2521 gtttcgaatg atactaacat aacatagaac attttcagga ggacccttgc ttggagggta
     2581 ccgagctcag aaaaaatgac cgctccaaag aagaagcgca aggttccagt cgagaagatg
     2641 actgccccaa aaaagaaacg taaggtcccg cgggtaagtt taaacagttc ggtactaact
     2701 aaccatacat atttaaattt tcagcgcgct gtgtcaaaag gagaagagct tattaaggag
     2761 aatatgcgtt ccaagctcta cctcgaggga tccgtcaacg gacaccaatt caagtgcacc
     2821 cacgagggag agggaaagcc atacgaggga aagcaaacca accgtatcaa ggtcgtcgag
     2881 ggaggaccac tcccattcgc cttcgacatc ctcgccaccc acttcatgta cggatccaag
     2941 gtaagtttaa acatatatat actaactaac cctgattatt taaattttca ggtcttcatc
     3001 aagtacccag ccgacctccc agactacttc aagcaatcct tcccagaggg attcacctgg
     3061 gagcgtgtca tggtcttcga ggacggagga gtcctcaccg ccacccaaga cacctccctc
     3121 caagacggag agctcatcta caacgtcaag gtccgtggag tcaacttccc agccaacgga
     3181 ccagtcatgc aaaagaagac cctcggatgg gagccatcca ccgagaccat gtacccagcc
     3241 gacggaggac tcgagggacg ttgcgacaag gtaagtttaa acatgatttt actaactaac
     3301 taatctgatt taaattttca ggccctcaag ctcgtcggag gaggacacct ccacgtcaac
     3361 ttcaagacca cctacaagtc caagaagcca gtcaagatgc caggagtcca ctacgtcgac
     3421 cgtcgtctcg agcgtatcaa ggaggccgac aacgagacct acgtcgagca atacgagcac
     3481 gccgtcgccc gttactccaa cctcggagga ggaatggacg agctctacaa gcctaggata
     3541 ccggtgggtg aagacccacc aaagccatct gccaagggag ccaagaaggc cgccaagacc
     3601 gttacgaagc caaaggacgg aaagaagaga cgtcatgccc gtaaggaatc atactccgtc
     3661 tacatctacc gtgtcctcaa gcaagttcat ccagacactg gagtttcctc caaagccatg
     3721 tctatcatga actcttttgt caacgatgtc ttcgagcgta ttgctgctga agcatcccgt
     3781 cttgctcact acaacaagcg ttccacaatc tcatcccgcg aaattcagac cgctgtccgt
     3841 ctgatccttc caggagagct tgccaagcac gccgtgtctg agggaaccaa ggccgttacc
     3901 aagtacactt ccagcaagta ggaattccaa ctgagcgccg gtcgctacca ttaccaactt
     3961 gtctggtgtc aaaaataata ggggccgctg tcatcagagt aagtttaaac tgagttctac
     4021 taactaacga gtaatattta aattttcagc atctcgcgcc cgtgcctctg acttctaagt
     4081 ccaattactc ttcaacatcc ctacatgctc tttctccctg tgctcccacc ccctattttt
     4141 gttattatca aaaaacttct cttaatttct ttgtttttta gcttctttta agtcacctct
     4201 aacaatgaaa ttgtgtagat tcaaaaatag aattaattcg taataaaaag tcgaaaaaaa
     4261 ttgtgctccc tccccccatt aataataatt ctatcccaaa atctacacaa tgttctgtgt
     4321 acacttctta tgttttttac ttctgataaa tttttttgaa acatcataga aaaaaccgca
     4381 cacaaaatac cttatcatat gttacgtttc agtttatgac cgcaattttt atttcttcgc
     4441 acgtctgggc ctctcatgac gtcaaatcat gctcatcgtg aaaaagtttt ggagtatttt
     4501 tggaattttt caatcaagtg aaagtttatg aaattaattt tcctgctttt gctttttggg
     4561 gtttccccta ttgtttgtca agatttcgag gacggcgttt ttcttgctaa aatcacaagt
     4621 attgatgagc acgatgcaag aaagatcgga agaaggtttg ggtttgaggc tcagtggaag
     4681 gtgagtagaa gttgataatt tgaaagtgga gtagtgtcta tggggttttt gccttaaatg
     4741 acagaataca ttcccaatat accaaacata actgtttcct actagtcggc cgtacgggcc
     4801 ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga
     4861 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc
     4921 agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact
     4981 gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat
     5041 caggcggcct taagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
     5101 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     5161 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     5221 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     5281 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     5341 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     5401 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     5461 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     5521 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     5581 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     5641 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     5701 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     5761 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     5821 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     5881 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     5941 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     6001 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     6061 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     6121 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     6181 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     6241 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     6301 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     6361 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     6421 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     6481 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     6541 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     6601 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     6661 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     6721 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     6781 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     6841 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     6901 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     6961 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     7021 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     7081 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     7141 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
     7201 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatt
//
