LOCUS       sequence-376113-        9092 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  flp-7 fluorescent neural reporter driving nuclear CYOFP expression 
            (refer to NeuroPAL paper for expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9092)
  AUTHORS   Yemini E, Lin A, Nejatbakhsh A, Varol E, Sun R, Mena GE, Samuel ADT,
            Paninski L, Venkatachalam V, Hobert O
  TITLE     NeuroPAL: A Multicolor Atlas for Whole-Brain Neuronal Identification
            in C. elegans.
  JOURNAL   Cell. 2021 Jan 7;184(1):272-288.e11. doi: 
            10.1016/j.cell.2020.12.012. Epub 2020 Dec 29.
  PUBMED    33378642
REFERENCE   2  (bases 1 to 9092)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.012"; journalName: "Cell"; date: "2021-01-7-
            7"; volume: "184"; issue: "1"; pages: "272-288.e11"
COMMENT     Sequence Label: pEY82
FEATURES             Location/Qualifiers
     source          1..9092
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6..22
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             4451..4471
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4493..4513
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(6683..6705)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6805..6824
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6902..6920)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6988..7092
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7093..7161,7162..7953)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7311..7330)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8124..8712
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8613..8632
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8866..8883
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9000..9021
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9036..9041,9042..9059,9060..9066)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9074..9090
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(9079..9092,1..9)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcctgcagt tctcggccaa
      181 atctatgtat ttcgtgtaaa taaacaaatg ttcatcagaa agtcacttca gcttcaagtc
      241 ttttatttat tttattgctt tacgtgaata ctgattttaa gacttttttc atatttttct
      301 atacgaatac gaaaatgttg aacgacttct gaaaaacaac atttctttag gaatttcaat
      361 agtttagtag tgatgcatta attagtagat gacacttttc taagtctttc ttttttaaaa
      421 gagtttcaca aagaaacgtt cgatctattt ttctactcat tcagttcgta aaaattggat
      481 tatttccatg ttttttgtta gaatttccta ttcaagtatc ttgtcactta taaaatgaaa
      541 gaaaacgtca gaaattaacg cgtattctaa gaaatattac cggaaggtga ttcagtctag
      601 cgtgaagctg gagaaattac acgttttctc atcaattaca ctgtaagaag cgtaaaagag
      661 cagcgagtga attttcataa tattttactt ttgtgtctct tgacctgaaa gacttgagga
      721 aagagatgga aataaatgaa tctactccgt atttttgcaa aattaacgtt cagactagat
      781 aactgttcca gaaaaccagc atgtcatttt tcaaataaaa taatcaggct aacttgaact
      841 ttctgattct tgttgcaata ccgtttttgg taaagttttg caaattggtc gataatctga
      901 aaattgcaga aaagtagagt tacccgtcgt ctctgacata actttttttt ttcatgaaga
      961 ttctgtaaaa ccagcagcgc ctgccttgcc taaaagatca attcgcgcct tctgtatagt
     1021 gcggcgcgga acctgtcggc tgttgcatat cgcactatgc ggtcattaca cgtcaaaatg
     1081 cagaattgat gcgatgaggc cgtcgtgagg cctacagctt ttaggaagtc gcctggccgt
     1141 aactgtgttt atctcacaag agcaacaatc gctcaagttt gcttttgaaa caaagcgaat
     1201 attttctaga cctttaaaca ttttcaaaaa tactccttct gatattctac aaatataatt
     1261 tcttaggatt agtaacattg tccctgtaaa ctttacgacc ttactatgta tcagcctgaa
     1321 aaaaaattga aaaataaaac atctgcgaag tccatgcttt cttgcacgtt ttattatgca
     1381 atttctcgtc attgtgattt tttgtttcta ttcccatctg cttgcaacta tttttttact
     1441 cataactctc cgctgattat tcctccccat tgtgactcta tgatcgcaga aactaataaa
     1501 aagataatga cgaatcccaa attttagatg actttttagg catcttccat gctcaatgag
     1561 cttctcacgt tctaatcaaa aactgcttct tcccccgcat cctcataaaa acaggctagt
     1621 attactcata tcggtgccca tttttgactt tattctattc aacatgaatt cccttgtcgc
     1681 agttgaatca aaaaataaaa taaacatgac gttatgattt caataaaaac tatgagtaga
     1741 tgacaactac tacatgttga tcagcagtac tctaagaagt gcacttcgga aagatatttc
     1801 aagtgccaaa cgtttttcta gaatttagaa agctttacgc acagaaaaat attttgattt
     1861 ctcactataa aaactaaaaa taaaatctaa aagcttgaca ttttgatggc gaactatgaa
     1921 aatatgaaaa tcaaatctcc aacatctcaa aacaattttt caactgtcaa gtgttcatct
     1981 tcattctttt cttttttatt ttttttctga tttttgttgc tcatttttct ttaacccgct
     2041 tttccaggag ttctccaaaa aaccctcgct gttagctata aatcaattta ttttgggagt
     2101 acatgttgcc acgaaaaata aaacaaagta attttctaag aaatcggaat aaatttttta
     2161 cccttattaa aaactactac gtcgattaat gttaaacaaa tttaatcata catgtttcta
     2221 gcgaaaaaaa aaccttgaat acggtcaaag aacagcgaat tctgataagg catattttct
     2281 atgtttattt attagtgttg cttgaataaa ttcatgatca tttatagtga tgcgagagct
     2341 gaaagtttct tggagcgaaa caaaaagcaa aattggagcc tatataacca tgtgatcaaa
     2401 gtgtcaaaat gatttaacaa ttgtagtttc ataaactaga aattacgtgt ggaaactgac
     2461 aaaaactttc aacttttcaa cttttccgcc ttcaattttt ctagaattct taatttcatt
     2521 gcagcagcga cttaatttcc gttttgacgt agacttaaga ttatatcggt aacattagtt
     2581 gtaagttttc ttcgtcattt tacaaagact tacaactcgc cgtataaatt ttcaattttt
     2641 tcggaattgt tggcccacgt gttttcattt attgtgaaca aagttaatag cttggttgtg
     2701 tctggaaaag ttacttaaag tagttcaaaa acttagagtt ctatgaaata gcagcttctc
     2761 ttctggcatc tctcacatct tttgctcgag ttacgataat tgaggaaacg tttgaaatta
     2821 aatgcacagg tgattctata aaatacttat ggaatgtttt tgaaagggtt ttgcaaaaat
     2881 actcaaacgc atctacaata agtgtacata cgtaatatga catatcaccc agattcacaa
     2941 aaccacatgt tttatagata tggtacttgg tttcaacaat ttttaacata ctttgaaaaa
     3001 gagcaaaaag tatgttttac attatttatt gaaaactcgt gtcaagacct gttattgtac
     3061 ttaagttttt tatctgaaac tattttaaac tctacatttc acaatcaatg atcaaaagaa
     3121 aaactgaccg aaatttcttt ttttttattt taaactagtt tttgaacaaa attgaactta
     3181 aaaaacttca gtatccacaa ttgaaaaaaa acgtcatttt ttgattttct taatcttgaa
     3241 gctgtattgt aatgtactta aaattatttt tgttatgcga ctcttataat attttacata
     3301 aaaaggacta gataaatatg tgtttgtgca tcacaacccc cgtttaaaaa taaattgaac
     3361 tttcattcat ttattcattt caattaaaca taatatattt tataattttt tttcaggctc
     3421 caaaatgact tttttatagg ttgaagagcc tgataatagt tttaataata cctaaattta
     3481 taaattgaaa tttcaacgta catcagtacg gataactttg tgtctaagta atacatacaa
     3541 aatttttgga aacttatttg ttgaattaat ttttattttt gccttcttct aacacttcct
     3601 cctaaattct caactgaccg ctcaaaaggt ttcaaacatc tttctctttg ctcttctgga
     3661 gagtgcttgc tagtatccta aaatacccga cacaatcaaa cagagatgga ccgcgtgaat
     3721 aatgaagtgt cccaagatcc gtataattca ctttgattgc tctgtgaact tggatttttt
     3781 taatcgggct aatgcgtctt tagcaaaaat agccaaccat ctcaatggat tccaacctaa
     3841 ttttgatttt tttttttttt ggtgttattt tttctccttg ttccgatttc cagtttttgt
     3901 ttggacgaag gcggagtcat agcatcagac aaggttagaa ggcatgagaa gaagggggtg
     3961 gagcatgagc ctttctcgag ctcaggaata tgaaagcgta tataagtatg gatagcagct
     4021 tctattgcgt ttctgattgt gattattttg ccccgtcatc agttttcttt tcagtaaatt
     4081 gtcaaataca aattttttga ttactgttgc ttggtcttgt gcaatccact caatcttgaa
     4141 tccaatttgc agttcaagtt acaaattgaa tttccctctg ccaacactca aagaagggtt
     4201 cttttttttg acttttcaac ttttctcctc attttttctc gattgaaatt tcaattttta
     4261 aaccactccg cgtcatgttt ggaaacatga cgtcatcgaa aaataccatc tcgttttcat
     4321 tcaaagactt ttagaacccg ggattggcca aaggacccaa aggtatgttt cgaatgatac
     4381 taacataaca tagaacattt tcaggaggac ccttgcttgg agggtaccga gctcagaaaa
     4441 aatgaccgct ccaaagaaga agcgcaaggt tccagtcgag aagatgactg ccccaaaaaa
     4501 gaaacgtaag gtcccgcggg taagtttaaa cagttcggta ctaactaacc atacatattt
     4561 aaattttcag cgcgctgtgt caaaaggaga agagcttatt aaggagaata tgcgttccaa
     4621 gctctacctc gagggatccg tcaacggaca ccaattcaag tgcacccacg agggagaggg
     4681 aaagccatac gagggaaagc aaaccaaccg tatcaaggtc gtcgagggag gaccactccc
     4741 attcgccttc gacatcctcg ccacccactt catgtacgga tccaaggtaa gtttaaacat
     4801 atatatacta actaaccctg attatttaaa ttttcaggtc ttcatcaagt acccagccga
     4861 cctcccagac tacttcaagc aatccttccc agagggattc acctgggagc gtgtcatggt
     4921 cttcgaggac ggaggagtcc tcaccgccac ccaagacacc tccctccaag acggagagct
     4981 catctacaac gtcaaggtcc gtggagtcaa cttcccagcc aacggaccag tcatgcaaaa
     5041 gaagaccctc ggatgggagc catccaccga gaccatgtac ccagccgacg gaggactcga
     5101 gggacgttgc gacaaggtaa gtttaaacat gattttacta actaactaat ctgatttaaa
     5161 ttttcaggcc ctcaagctcg tcggaggagg acacctccac gtcaacttca agaccaccta
     5221 caagtccaag aagccagtca agatgccagg agtccactac gtcgaccgtc gtctcgagcg
     5281 tatcaaggag gccgacaacg agacctacgt cgagcaatac gagcacgccg tcgcccgtta
     5341 ctccaacctc ggaggaggaa tggacgagct ctacaagcct aggataccgg tgggtgaaga
     5401 cccaccaaag ccatctgcca agggagccaa gaaggccgcc aagaccgtta cgaagccaaa
     5461 ggacggaaag aagagacgtc atgcccgtaa ggaatcatac tccgtctaca tctaccgtgt
     5521 cctcaagcaa gttcatccag acactggagt ttcctccaaa gccatgtcta tcatgaactc
     5581 ttttgtcaac gatgtcttcg agcgtattgc tgctgaagca tcccgtcttg ctcactacaa
     5641 caagcgttcc acaatctcat cccgcgaaat tcagaccgct gtccgtctga tccttccagg
     5701 agagcttgcc aagcacgccg tgtctgaggg aaccaaggcc gttaccaagt acacttccag
     5761 caagtaggaa ttccaactga gcgccggtcg ctaccattac caacttgtct ggtgtcaaaa
     5821 ataatagggg ccgctgtcat cagagtaagt ttaaactgag ttctactaac taacgagtaa
     5881 tatttaaatt ttcagcatct cgcgcccgtg cctctgactt ctaagtccaa ttactcttca
     5941 acatccctac atgctctttc tccctgtgct cccaccccct atttttgtta ttatcaaaaa
     6001 acttctctta atttctttgt tttttagctt cttttaagtc acctctaaca atgaaattgt
     6061 gtagattcaa aaatagaatt aattcgtaat aaaaagtcga aaaaaattgt gctccctccc
     6121 cccattaata ataattctat cccaaaatct acacaatgtt ctgtgtacac ttcttatgtt
     6181 ttttacttct gataaatttt tttgaaacat catagaaaaa accgcacaca aaatacctta
     6241 tcatatgtta cgtttcagtt tatgaccgca atttttattt cttcgcacgt ctgggcctct
     6301 catgacgtca aatcatgctc atcgtgaaaa agttttggag tatttttgga atttttcaat
     6361 caagtgaaag tttatgaaat taattttcct gcttttgctt tttggggttt cccctattgt
     6421 ttgtcaagat ttcgaggacg gcgtttttct tgctaaaatc acaagtattg atgagcacga
     6481 tgcaagaaag atcggaagaa ggtttgggtt tgaggctcag tggaaggtga gtagaagttg
     6541 ataatttgaa agtggagtag tgtctatggg gtttttgcct taaatgacag aatacattcc
     6601 caatatacca aacataactg tttcctacta gtcggccgta cgggcccttt cgtctcgcgc
     6661 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
     6721 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
     6781 ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata
     6841 tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cggccttaag
     6901 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
     6961 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     7021 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     7081 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     7141 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     7201 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     7261 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     7321 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     7381 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     7441 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     7501 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     7561 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     7621 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     7681 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     7741 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     7801 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     7861 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     7921 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     7981 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     8041 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     8101 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     8161 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     8221 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     8281 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     8341 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     8401 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     8461 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     8521 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     8581 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     8641 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     8701 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     8761 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     8821 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     8881 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     8941 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
     9001 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
     9061 atgttgtgtg gaattgtgag cggataacaa tt
//
