LOCUS       sequence-376114-        6304 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ggr-1 fluorescent neural reporter driving nuclear CYOFP expression 
            (refer to NeuroPAL paper for expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6304)
  AUTHORS   Yemini E, Lin A, Nejatbakhsh A, Varol E, Sun R, Mena GE, Samuel ADT,
            Paninski L, Venkatachalam V, Hobert O
  TITLE     NeuroPAL: A Multicolor Atlas for Whole-Brain Neuronal Identification
            in C. elegans.
  JOURNAL   Cell. 2021 Jan 7;184(1):272-288.e11. doi: 
            10.1016/j.cell.2020.12.012. Epub 2020 Dec 29.
  PUBMED    33378642
REFERENCE   2  (bases 1 to 6304)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.012"; journalName: "Cell"; date: "2021-01-7-
            7"; volume: "184"; issue: "1"; pages: "272-288.e11"
COMMENT     Sequence Label: pEY88
FEATURES             Location/Qualifiers
     source          1..6304
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6..22
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1663..1683
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1705..1725
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(3895..3917)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4017..4036
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4114..4132)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4200..4304
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4305..4373,4374..5165)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4523..4542)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5336..5924
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5825..5844
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6078..6095
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6212..6233
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6248..6253,6254..6271,6272..6278)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6286..6302
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(6291..6304,1..9)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcctgcaga cagcatgttt
      181 gtagcgcaca aaacgaaagt gaacaacgtg caaaaagaca gcccagtggt gttttgttat
      241 ctcgaaagat gcagaaatac gaagagacgc agcgagtgta gcaacgcgcg gtcattccgc
      301 aacaggactt tgacctcttc ctcgtcttat tgtactttgt attgtactct ctggaatttt
      361 tatgtaggca cactgtaggc aaccgtgtgc tctggcggaa atcaaaagtt cttgattaaa
      421 agaggcatga acaaaaatga acaaatttta taataacatt tcttttgaaa aaaaagtaaa
      481 aatttgaaat aggaaactac cgtatttcct ctaatagtga gagtatcgaa ctttaactga
      541 cgtgagatac aaggtgtcaa agttgaacac tggggtgaaa tactaataga ggaattatat
      601 cattaagaaa atatattgag tctatcacgg tggaaaattt tgaaccactc taaccctact
      661 attttttata gaaggaagtc tatactgaaa gttaatcaat caaataaata ctataaatat
      721 tttgggatcg aatttttgtt tggcaataac agccaagtcg agtaaattat gtaggtatta
      781 gataaaataa ttttagactg atagctaatc aaatttatag aaaatgaagt tcaaatcaag
      841 gcgggaaaat tgaaactttc tagcattggt tgaaaattct cgacaaggtc aacaatcagc
      901 ctgtttgaac tccctccaaa ataaaaactg caaaaccaac cagcgatttg ctccgctctt
      961 ttttcaacca atcaactgga gtttgtctat tttcgacgat actgattggt ttgaaactag
     1021 gcttctcatt ttggaaacaa atttcaacag acagggctat catttaaata cagctggaaa
     1081 atcgcggttc caaaagttgg atttttaatt tttaatttta atttttttct tttcgatttt
     1141 tacacgtcaa gaaaaataat ttcagtcagt agtatgatat cttcgactac actttccttt
     1201 aattctttca tccgtaataa tcatgtttcg aagcatgcgg gattattacc tttaatcaaa
     1261 taatatactt ttaaagtaca tccgatgctc cttccaacag tgtcctccaa aatcttccca
     1321 aaatccatcg atgagttcat tctcccattc tcccacttcc cacatttctt tcatcctttc
     1381 tgctgtcgcg cccctaaacc ccgcccattt tctgttatgc ccctcgcttt gagagcttct
     1441 gcttcttctc cttctgcccc agttccacca ctacacttct tctttcttag aggcttctgc
     1501 ttaattccaa ccaaaaattc atattcaact gcatacttta attattgacc cgggattggc
     1561 caaaggaccc aaaggtatgt ttcgaatgat actaacataa catagaacat tttcaggagg
     1621 acccttgctt ggagggtacc gagctcagaa aaaatgaccg ctccaaagaa gaagcgcaag
     1681 gttccagtcg agaagatgac tgccccaaaa aagaaacgta aggtcccgcg ggtaagttta
     1741 aacagttcgg tactaactaa ccatacatat ttaaattttc agcgcgctgt gtcaaaagga
     1801 gaagagctta ttaaggagaa tatgcgttcc aagctctacc tcgagggatc cgtcaacgga
     1861 caccaattca agtgcaccca cgagggagag ggaaagccat acgagggaaa gcaaaccaac
     1921 cgtatcaagg tcgtcgaggg aggaccactc ccattcgcct tcgacatcct cgccacccac
     1981 ttcatgtacg gatccaaggt aagtttaaac atatatatac taactaaccc tgattattta
     2041 aattttcagg tcttcatcaa gtacccagcc gacctcccag actacttcaa gcaatccttc
     2101 ccagagggat tcacctggga gcgtgtcatg gtcttcgagg acggaggagt cctcaccgcc
     2161 acccaagaca cctccctcca agacggagag ctcatctaca acgtcaaggt ccgtggagtc
     2221 aacttcccag ccaacggacc agtcatgcaa aagaagaccc tcggatggga gccatccacc
     2281 gagaccatgt acccagccga cggaggactc gagggacgtt gcgacaaggt aagtttaaac
     2341 atgattttac taactaacta atctgattta aattttcagg ccctcaagct cgtcggagga
     2401 ggacacctcc acgtcaactt caagaccacc tacaagtcca agaagccagt caagatgcca
     2461 ggagtccact acgtcgaccg tcgtctcgag cgtatcaagg aggccgacaa cgagacctac
     2521 gtcgagcaat acgagcacgc cgtcgcccgt tactccaacc tcggaggagg aatggacgag
     2581 ctctacaagc ctaggatacc ggtgggtgaa gacccaccaa agccatctgc caagggagcc
     2641 aagaaggccg ccaagaccgt tacgaagcca aaggacggaa agaagagacg tcatgcccgt
     2701 aaggaatcat actccgtcta catctaccgt gtcctcaagc aagttcatcc agacactgga
     2761 gtttcctcca aagccatgtc tatcatgaac tcttttgtca acgatgtctt cgagcgtatt
     2821 gctgctgaag catcccgtct tgctcactac aacaagcgtt ccacaatctc atcccgcgaa
     2881 attcagaccg ctgtccgtct gatccttcca ggagagcttg ccaagcacgc cgtgtctgag
     2941 ggaaccaagg ccgttaccaa gtacacttcc agcaagtagg aattccaact gagcgccggt
     3001 cgctaccatt accaacttgt ctggtgtcaa aaataatagg ggccgctgtc atcagagtaa
     3061 gtttaaactg agttctacta actaacgagt aatatttaaa ttttcagcat ctcgcgcccg
     3121 tgcctctgac ttctaagtcc aattactctt caacatccct acatgctctt tctccctgtg
     3181 ctcccacccc ctatttttgt tattatcaaa aaacttctct taatttcttt gttttttagc
     3241 ttcttttaag tcacctctaa caatgaaatt gtgtagattc aaaaatagaa ttaattcgta
     3301 ataaaaagtc gaaaaaaatt gtgctccctc cccccattaa taataattct atcccaaaat
     3361 ctacacaatg ttctgtgtac acttcttatg ttttttactt ctgataaatt tttttgaaac
     3421 atcatagaaa aaaccgcaca caaaatacct tatcatatgt tacgtttcag tttatgaccg
     3481 caatttttat ttcttcgcac gtctgggcct ctcatgacgt caaatcatgc tcatcgtgaa
     3541 aaagttttgg agtatttttg gaatttttca atcaagtgaa agtttatgaa attaattttc
     3601 ctgcttttgc tttttggggt ttcccctatt gtttgtcaag atttcgagga cggcgttttt
     3661 cttgctaaaa tcacaagtat tgatgagcac gatgcaagaa agatcggaag aaggtttggg
     3721 tttgaggctc agtggaaggt gagtagaagt tgataatttg aaagtggagt agtgtctatg
     3781 gggtttttgc cttaaatgac agaatacatt cccaatatac caaacataac tgtttcctac
     3841 tagtcggccg tacgggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg
     3901 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca
     3961 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc
     4021 atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt
     4081 aaggagaaaa taccgcatca ggcggcctta agggcctcgt gatacgccta tttttatagg
     4141 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
     4201 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     4261 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     4321 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     4381 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     4441 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     4501 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     4561 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     4621 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     4681 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     4741 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     4801 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     4861 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     4921 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     4981 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     5041 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     5101 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     5161 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     5221 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     5281 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     5341 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     5401 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     5461 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     5521 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     5581 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     5641 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     5701 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     5761 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     5821 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     5881 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     5941 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     6001 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     6061 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     6121 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
     6181 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
     6241 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
     6301 aatt
//
