LOCUS       sequence-376117-        6828 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fluorescent reporter driving amphid and phasmid nuclear GCaMP6s 
            expression (refer to NeuroPAL paper for expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6828)
  AUTHORS   Yemini E, Lin A, Nejatbakhsh A, Varol E, Sun R, Mena GE, Samuel ADT,
            Paninski L, Venkatachalam V, Hobert O
  TITLE     NeuroPAL: A Multicolor Atlas for Whole-Brain Neuronal Identification
            in C. elegans.
  JOURNAL   Cell. 2021 Jan 7;184(1):272-288.e11. doi: 
            10.1016/j.cell.2020.12.012. Epub 2020 Dec 29.
  PUBMED    33378642
REFERENCE   2  (bases 1 to 6828)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.012"; journalName: "Cell"; date: "2021-01-7-
            7"; volume: "184"; issue: "1"; pages: "272-288.e11"
COMMENT     Sequence Label: pEY13
FEATURES             Location/Qualifiers
     source          1..6828
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6..22
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1865..1885
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1910..1930
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1952..1972
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1997..2014
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             join(2054..2062,2063..2077)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             2105..2161
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     primer_bind     complement(4419..4441)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4541..4560
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4638..4656)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4724..4828
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4829..4897,4898..5689)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5047..5066)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5860..6448
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6349..6368
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6602..6619
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6736..6757
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6772..6777,6778..6795,6796..6802)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6810..6826
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(6815..6828,1..9)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcctgcaga atgggcagct
      181 caacttcacg gtaaaatctc aaacttccta atgactttac aaactttaca tttattttca
      241 ggttaccgcc ccatgtgttg cttttcgtcc gggacaaaat gtcgaattga agtttaacat
      301 agaaaaccac agctccacaa ctgtaaagaa aatcttcacc gagctgaaaa cagaagccca
      361 tcatcatcat ttcaatcaaa tttttccgtg tagggatgcg tattcctgtc ctgtggcaga
      421 gaagtataat aaatacaaga cagtaggtga aactaaaaca atgaaagttt gtgtggcacc
      481 gtattcaact ggaacattca cgatgccgtt tacgatccct gagaatttaa cgacaggttt
      541 cgcaactgga attatgactt tgagatatta tatggcattt ggagtggtaa ctaactcatg
      601 gctcacgatg gatccagtgg catttctctc agtctttatt ggaaaacggg aaattgacga
      661 gaagaatgat gaacacgtcg gaagacggaa gacagctttg tttgggacat tgatacaatt
      721 aaaaattaaa atagattttt cgaacttgat tcactaatat ttgtaacctc aattttcaaa
      781 ttctgtttta ctccaatgct atcacatcct aacaacaatt gttatgcttt tgtttttttc
      841 tcttaaagaa ataaagttgt tttttgttaa cttgatgagc cacggtcaaa tatgtactta
      901 aaggaaaagt ttacgaactt tgaacaaggt tgggcaataa gttgcagcaa actgccggtt
      961 ggctgaaacg acgaggtttg taaacgtaga catcagattg atctactgtt gaattttagg
     1021 aagtttcaaa gctgttgtct cgaagatgtt gttagaaagt gaatttaatg tgtgccactc
     1081 taacttaatg aaacgctgtt ttttcgagca ctatatctag tttgttttca taaatataac
     1141 ttcaaatggc atttcatata ttccacaaac ttcgaagcga tatttaaaat tgttgttcag
     1201 ttgttggagt aaacagcaac gccacttatc ctctaataca tcatgtgctc ctctcttatt
     1261 attctctctc gaactcaaat ttgattatag ttattactta attcgaggga gagaagacga
     1321 gagtgatgaa taaggctaga atggcttaga cattatagta gtcagagaga cacagagata
     1381 aagaagagtc ctaaacctaa atgagcagcg tgagaggcgg cgagacagct cttgtttgat
     1441 gagagggaag agtggtggtt gccattagtt acgccatcat tcacatgttt gtgctccgca
     1501 tttcagttct cgcatttgca gttgtttttg tttcttcatc gtcaagttgg aaacgcattt
     1561 ccgctctttt ctttgttttt tgttcttttg caaaaataat tggtttctag atatatgtat
     1621 ttctatgtat aacatttaac aataaattaa gagttatttt cttatcaaaa ttagcagaaa
     1681 attattttaa tatttttttt atatatttaa caaaatcact gaaaccccct ttcctcatga
     1741 gacctatttt ttcagtcccg ggattggcca aaggacccaa aggtatgttt cgaatgatac
     1801 taacataaca tagaacattt tcaggaggac ccttggaggg taccggtcag aaaaaatgac
     1861 tgctccaaaa aaaaaacgca aagttccatc atccgaaaag atgaccgccc caaagaagaa
     1921 gcgcaaggtc cctgtcgccg agaagactgc cccgaagaaa aagcgcaagg tcccatctgc
     1981 cgccgctatg ggatcccacc accaccatca tcacggaatg gcctccatga ctggaggaca
     2041 acaaatggga cgcgacctct acgacgacga cgacaaggac ctcgccacaa tggtcgactc
     2101 ctcccgtcgc aaatggaata agaccggaca cgccgtccgt gccatcggtc gtctctcctc
     2161 cctcgagaac gtctacatca aggccgacaa gcaaaagaac ggaatcaagg taagtttaaa
     2221 catatacata ctaactaacc ctgattattt aaattttcag gccaacttcc acatccgtca
     2281 caacatcgag gacggaggag tccaactcgc ctaccactac caacaaaaca ccccaatcgg
     2341 agacggacca gtcctcctcc cagacaacca ctacctctcc gtccaatcca agctctcaaa
     2401 ggacccaaac gagaaacgcg atcacatggt ccttcttgaa tttgtcactg ccgccggcat
     2461 tacactggga atggatgaac tctataaggg aggaaccgga ggatccatgg tctccaaagg
     2521 agaggagctc ttcaccggag tcgtcccaat cctcgtcgag ctcgacggag acgtcaacgg
     2581 tcacaagttc tccgtctccg gagagggaga gggagacgcc acctacggaa agctcaccct
     2641 caagttcatc tgcaccactg gaaagctccc agtcccatgg ccaaccctcg tcaccaccct
     2701 cacctacgga gtccaatgct tctcccgtta cccaggtaag tttaaagaga tcgatactaa
     2761 caaaccatac atatttaaat tttcagacca catgaagcaa cacgacttct tcaagtccgc
     2821 catgccagag ggatacatcc aagagcgtac catcttcttc aaggacgacg gaaactacaa
     2881 gacccgtgcc gaggtcaaat tcgagggaga caccctcgtc aaccgcatcg agctcaaggg
     2941 aatcgacttc aaggaggacg gaaacatcct cggacacaag ctcgaataca acctcccaga
     3001 ccaactcacc gaggagcaaa tcgctgaatt caaagaggcc ttctcacttt ttgacaagga
     3061 cggagacgga accatcacca ctaaggtaag tttaaagaag attttactaa ctaactaatc
     3121 tgatttaaat tttcaggagc tcggaaccgt catgcgttcc ctcggacaaa acccaaccga
     3181 ggccgagctc caagacatga tcaacgaggt cgacgccgac ggagacggaa ccatcgactt
     3241 cccagagttc ctcaccatga tggcccgcaa gatgaagtac cgcgacactg aagaggagat
     3301 tcgcgaagcc ttcggagtct tcgacaagga cggaaacgga tacatctccg ccgccgagct
     3361 ccgtcacgtc atgaccaacc tcggagagaa gctcaccgac gaggaggtcg acgagatgat
     3421 ccgcgaggcc gacatcgacg gagacggaca agtcaactac gaggaattcg tccaaatgat
     3481 gaccgccaag gcctaggata ccggaattcc aactgagcgc cggtcgctac cattaccaac
     3541 ttgtctggtg tcaaaaataa taggggccgc tgtcatcaga gtaagtttaa actgagttct
     3601 actaactaac gagtaatatt taaattttca gcatctcgcg cccgtgcctc tgacttctaa
     3661 gtccaattac tcttcaacat ccctacatgc tctttctccc tgtgctccca ccccctattt
     3721 ttgttattat caaaaaactt ctcttaattt ctttgttttt tagcttcttt taagtcacct
     3781 ctaacaatga aattgtgtag attcaaaaat agaattaatt cgtaataaaa agtcgaaaaa
     3841 aattgtgctc cctcccccca ttaataataa ttctatccca aaatctacac aatgttctgt
     3901 gtacacttct tatgtttttt acttctgata aatttttttg aaacatcata gaaaaaaccg
     3961 cacacaaaat accttatcat atgttacgtt tcagtttatg accgcaattt ttatttcttc
     4021 gcacgtctgg gcctctcatg acgtcaaatc atgctcatcg tgaaaaagtt ttggagtatt
     4081 tttggaattt ttcaatcaag tgaaagttta tgaaattaat tttcctgctt ttgctttttg
     4141 gggtttcccc tattgtttgt caagatttcg aggacggcgt ttttcttgct aaaatcacaa
     4201 gtattgatga gcacgatgca agaaagatcg gaagaaggtt tgggtttgag gctcagtgga
     4261 aggtgagtag aagttgataa tttgaaagtg gagtagtgtc tatggggttt ttgccttaaa
     4321 tgacagaata cattcccaat ataccaaaca taactgtttc ctactagtcg gccgtacggg
     4381 ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg
     4441 gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg
     4501 tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta
     4561 ctgagagtgc accatatgcg gtgcgaaata ccgcacagat gcgtaaggag aaaataccgc
     4621 atcaggcggc cttaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
     4681 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     4741 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     4801 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     4861 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     4921 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     4981 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     5041 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     5101 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     5161 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     5221 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     5281 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     5341 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     5401 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     5461 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     5521 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     5581 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     5641 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     5701 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     5761 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     5821 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     5881 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     5941 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     6001 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     6061 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     6121 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     6181 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     6241 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     6301 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     6361 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     6421 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     6481 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     6541 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     6601 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc
     6661 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
     6721 tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt
     6781 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatt
//
